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1353 Effect of different doses of a -based probiotic on the in vitro digestibility of

concentrates and forages.

Article  in  Journal of Animal Science · January 2016

DOI: 10.2527/jam2016-1353

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 Effect of different doses of a Bacillus-based probiotic on the in vitro digestibility of
concentrates and forages.

C. A. Oliveira1 , D. O. Sousa1 , J. F. Penso1 , P. F. Menegucci2 , and L. F. P. Silva*1, 1

University of Sao Paulo, Pirassununga, Brazil, 2 Chr. Hansen, Valinhos, Brazil.

The objective was to evaluate the effect of different doses of probiotic containing 1.6x109
CFU/g of Bacillus licheniformis and 1.6x109 UFC/g of Bacillus subtilis (BioPlus® PS,
Chr. Hansen) on in vitro digestibility of concentrates and forages. The feedstuffs analyzed
included 2 types of concentrates: corn and sorghum, and 2 types of forages: sugarcane
silage and Megathirsus maximus cv. Mombaça (mombaça-grass). The probiotic was added
to the ruminal fuid simulating doses of 0, 1, 2, and 3 g head-1 d-1 . The doses of probiotic
added to the rumen fuid was calculated supposing a volume of L of rumen fuid per
animalDry matter, NDF, and starch in vitro digestibility of feedstuffs were determined in
0.5 g of sample, in triplicate, after incubation for 6 and 12 h (concentrates); or for 24 and 48
h (forages), using the Daisy Incubator (ANKOM®Technology Corp., Fairpoint, NY, USA).
The residues after fermentation were analyzed for NDF in the case of forages, and for
starch in the case of concentrates. The experiment was repeated three times. Statistical
model included the fxed effect of probiotic, feedstuff, and interaction between both factors,
with the experiment repetitions as the random effect. Addition of probiotic improved 24-h
NDF digestibility of both roughages (P = 0, 05), without a Dose × Feedstuff interaction (P
= 0.36). There was a cubic effect of dose of probiotic on 24-h NDF digestibility, with the
dose of 1 g head-1 d-1 promoting higher NDFD than the control (20.2 vs. 16.3%, P = 0.01).
When analyzed after 48 h of incubation, there was a signifcant Dose î Feedstuff interaction
(P < 0.01). Addition of 1 g head-1 d-1 of probiotic increased (P < 0.01) 48-h digestibility of
mombaça-grass, but not of sugarcane silage (P = 0.73). Considering the effects on starch
digestibility of concentrates, there was no effect of probiotic after 6 h of incubation (P >
0.05). However, after 12 h of incubation, there was a positive linear effect of addition of
probiotic on starch digestibility of both concentrates (P < 0.01), with no Dose × Feedstuff
interaction (P = 0.50). The addition of 3 g head-1 d-1 of probiotic increased by 10.9%
starch digestibility after 12 h of incubation. In conclusion, addition of increasing doses of a
bacillus based probiotic promoted a cubical increase in NDF digestibility of roughages, and
a linear increased in starch digestibility of concentrates. Key Words: fber digestibility,
starch digestibility, probiotic

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