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II B.Tech II Semester Regular Examinations, Apr/May 2008
INSTRUMENTAL METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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Code No: R05222302 Set No. 1
(a) matrix technique
(b) gyromagnetic ratio
(c) limitations of ESR. [16]
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Code No: R05222302 Set No. 2
II B.Tech II Semester Regular Examinations, Apr/May 2008
INSTRUMENTAL METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆
2. (a) Briefly describe the scanning probe microscope & its most popular versions
i.e the scanning tunneling microscope & the atomic force microscope.
(b) What are these microscopes used for? [8 × 2 = 16]
3. (a) In an experiment carried out at 200 , the following data was obtained when
congo red was subjected to sedimentation equilibrium :
X2 X1 C2 C1
5.87 5.84 53.6 50.46
The initial concentration of the dye was 0.1g per litre dissolved in 0.1 M Nacl,
the density of the resulting solution being 1.0023. The speed of rotation of
the centrifuge was 17,976 rpm. The partial specific volume of congo red is 0.6.
From the above data calculate the molecular weight of congo red.
(b) Explain the principle involved in above method. [8 × 2 = 16]
4. (a) Why does a deuterium lamp produces a continuous rather than a line spectrum
in the ultraviolet?
(b) What are the differences between a photon detector & a heat detector. [8×2=16]
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Code No: R05222302 Set No. 2
7. Define & explain the following:
8. (a) Why electron spin resonance is also called electron para magnetic resonance?
(b) Calculate the ESR frequency of an unpaired electron in a magnetic field 0.33τ .
Given for free electron g = 2&β = 9.273 × 10−24 JT −1 . [8 × 2 = 16]
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Code No: R05222302 Set No. 3
II B.Tech II Semester Regular Examinations, Apr/May 2008
INSTRUMENTAL METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆
3. (a) How are density gradiants solutions are prepared for centrifugation?
(b) Write a note on following materials importance:
i. Caesium chloride
ii. Sucrose
iii. Ficoll. [8 × 2 = 16]
5. (a) What are the differences between atomic absorption & flame emission.
(b) Describe the methods used to exite atoms. [8 × 2 = 16]
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Code No: R05222302 Set No. 4
II B.Tech II Semester Regular Examinations, Apr/May 2008
INSTRUMENTAL METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆
2. Who discovered the instrument Microscope? Give the principle on which this in-
strument works. [16]
3. Calculate the molecular mass of the protein where the sedimentation coefficient of
the protein is 7.75 ×10−13 s. In subsequent analysis the protein was found to have
an average diffusion coefficient 0f 4.0 × 10−11 m2 s−1 & the a partial specific volume
of 0. 734 ×10−3 m3 kg −1 .
7. (a) Calculate the NMR frequency of 19F ina magnetic field of 2.3487τ . Given
gn = 5.255&βn = 5.05 × 10−27 JT −1 .
(b) Describe NMR spectrum of ethanol & explain the position of each band.
[8 × 2 = 16]
8. Why ESR spectroscopy is widely used in the study of chemical, photochemical &
electrochemical reactions? Justify your answer. [16]
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