Service Manual

for
DS Series Fully Automatic Biochemistry
Analyzer

（VER. 4.2）

1
 Contents

Contents..................................................................................................................................... 2
Chapter 1 Brief Introduction of the Instrument......................................................................... 1
1.1 Basic theory of the instrument.............................................................................................1
1.2 Operating environment........................................................................................................ 1
1.3 Safety precautions................................................................................................................1
Chapter 2 Maintenance Personnel Requirements and Tools Required......................................2
2.1Maintenance personnel requirements........................................................................... 2
2.2 Knowledge that maintenance personnel need to know:.............................................. 2
2.3 Service tools.................................................................................................................2
Chapter 3 Instrument Maintenance....................................................................................2
3.1 Daily maintenance conditions......................................................................................2
3.1.1 Startup maintenance..................................................................................................2
3.1.2 Shutdown maintenance.............................................................................................3
3.2 Weekly maintenance.................................................................................................... 3
3.3 Monthly maintenance.................................................................................................. 3
3.4 Annual maintenance.....................................................................................................4
Chapter 4 Basic Structure and Main Parts of the Instrument.................................................... 5
4.1 Main control board...................................................................................................... 5
4.1.1 Basic functions..........................................................................................................5
4.1.2 Basic structure.......................................................................................................... 5
4.2 Motor drive module..................................................................................................... 6
4.2.1 Function.................................................................................................................... 6
4.2.2 Basic structure.......................................................................................................... 6
4.3 Power system............................................................................................................. 12
4.4 Analysis system.................................................................................................14
4.4.1 Optical system............................................................................................... 14
4.4.2 Heating system.............................................................................................. 14
4.4.3 Detection system............................................................................................17
4.4.4 Analysis of the points for attention in system maintenance and
replacement of parts.............................................................................................. 18
4.4.4.1 Adjustment of the reaction disk coded disc............................................. 18
4.4.4.2 Adjustment of optical fiber....................................................................... 19

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 4.4.4.3 Sensor replacement....................................................................................20
4.4.4.4 Replacement of optical filters................................................................... 21
4.5.1 Function......................................................................................................... 22
4.5.2 Installation methods of reagent and sample trays..................................... 23
4.5.2.1 Replacement of motors..............................................................................23
4.5.2.2 Belt adjustment and replacement.............................................................24
4.5.2.3 Replacement of cooling sheets.................................................................. 24
4.6 Liquid road system.......................................................................................... 25
4.6.1The function of the liquid road system........................................................ 25
4.6.2 The structure of the liquid road system......................................................25
Chapter 5 Correction and Replacement of Vulnerable Parts................................................... 27
5.1 Replacement of bulbs................................................................................................ 27
5.3 Replacement of reaction cups.................................................................................... 29
5.4 Replacement of power fuses...................................................................................... 29
5.5 Adjustment of the signal value GAIN and background value OFFSET....................29
5.5.1 Adjustment of the background value OFFSET.......................................................29
5.5.2 Adjustment of the signal value GAIN.................................................................... 31
Chapter 6 Common Faults and Troubleshooting..................................................................... 31
Appendix 1: Wiring Diagram of FA Series Instruments..........................................................44
1. Wiring Diagram of DS401 Motor Drive System......................................................... 44
2. Wiring Diagram of DS301 Motor Drive System......................................................... 45
Appendix II. Maintenance Manual for Electrolytes........................................................ 46
1. Fundamentals of the Electrolyte Analysis Module...................................................... 46
2. Maintenance of the Electrolyte Analysis Module........................................................46
3. Main Parts....................................................................................................................47
3.1. Main control board.........................................................................................47
3.1.1 Function......................................................................................................... 47
3.1.2 Basic structure(see F2.1).............................................................................. 47
3.3 Assembly........................................................................................................... 48
3.3.1 Function......................................................................................................... 48
3.3.2 For the basic structure, see F2.4.................................................................. 48
3.3.3 For the interior wiring sequence of serial ports, refer to F2.5..................49
4. Setting of Electrolyte Parameters................................................................................ 50
5. Upper Computer Software Operation..................................................................... 55

3
6. Replacement of instrument parts................................................................................. 58
7. Common Faults and Troubleshooting..........................................................................59

4
Chapter 1 Brief Introduction of the Instrument

1.1 Basic theory of the instrument

The test principle of the biochemical analyzer is based on the Lambert-Beer Law.

1.2 Operating environment

Normal operating environment shall meet the following conditions：

Ambient
10℃～30℃
temperature
Relative humidity 30％～70％
Atmospheric
860hPa～1060hPa
pressure
Power Equipped with regulated power supply, 220V～±10% ,50Hz±1
requirements Hz
External
No strong electromagnetic interference and mechanical
environmental
vibration, no corrosive gas, and no direct strong light exposure
requirements
Well-grounded

1.3 Safety precautions

The following safety precautions shall be noted during the installation and maintenance
of the instrument：
 There is 220V voltage in the machine. To operate it, firstly disconnect its power
supply to prevent electric shock.
 Do not let the liquid spill on the circuit board and power supply when maintaining
the liquid path part, to prevent burning the circuit.
 When the circuit section is maintained, the power must be cut off. Hot-line work is
strictly forbidden.
 Beware of biological contamination when maintaining the instrument.
 The instrument must be well grounded.
 Any liquid is forbidden in the heating ring.

1
Chapter 2 Maintenance Personnel Requirements and Tools
Required

2.1Maintenance personnel requirements

Biochemical instruments belong to precision instruments. Instrument maintenance personnel

must be after-sales engineers of our company or qualified professional personnel trained by
our company.

2.2 Knowledge that maintenance personnel need to know:

1. Basic knowledge of analog and digital circuits

2. Mastery of the basic performance and test methods of basic components
3. Relevant computer knowledge
4. Certain knowledge of maintenance safety

2.3 Service tools

Tool list: 1 medium size cross head screwdriver, 1 small size cross head screwdriver, 1
medium size flat head screwdriver, 1 small size flat head screwdriver, 1 pair of nipper pliers,
1 150×19 wrench, 1 set of Allen wrench, 1 150W electric soldering iron, 1 coil of soldering
tin, and 1 digital multimeter.

Chapter 3 Instrument Maintenance

3.1 Daily maintenance conditions

Daily maintenance is a daily process that must be carried out in the use of the instrument, and
is divided into startup maintenance and shutdown maintenance.

3.1.1 Startup maintenance

Turn on the power switch of the main machine, turn on the computer check whether the
deionized water is enough. Empty the waste liquid container, to make the waste liquid tube
droop naturally.
Open the operating interface of the instrument preheat it for 30 minutes before operating
the instrument carry out daily maintenance clean all reaction cups enter the
blank test interface of the cup conduct water injection test (3 times, save each time)

2
 directly pump water or perform the preset startup process
The following items need to be emphatically checked：
1) There is enough water in the bucket for the test
2）Whether the water in the reaction cup reaches a half of the cup to 60%; in case of the water
level below the half, the water injection rate needs to be adjusted
3）If more than 30% of the cups show blue, it is recommended to replace all the reaction cups
Note: For the first use of the installed instrument, the probe shall be cleaned 4 times and for 3
cycles to ensure that the bubble release box is filled with water.

3.1.2 Shutdown maintenance

Clean all reaction cups click on the daily maintenance icon clean the probe four
times infuse the reaction disc with water shut down the instrument or execute the
default shutdown maintenance process.

3.2 Weekly maintenance

1. Probe maintenance shall be carried out once a week. Adding sample probe tips and
cleaning head probes can be cleaned with alcohol gauze.
2. Clean all reagent bottles and purified water buckets to avoid crystallization.
3. Wipe away any dust or dirt on the surface of the instrument.

3.3 Monthly maintenance

Check the use of the reaction cup, soak and clean the reaction cup with the cleaning liquid,
take out the reaction cup, wipe the outer surface of the reaction cup manually with the lens
paper, and at the same time, avoid scratching the detection surface during the cleaning
process, and replace the reaction cup if necessary.
Check the circulating pump coolant: unscrew the screw on the circulating pump
counterclockwise. If the coolant in the circulating pump is less than one third of the vessel, it
is necessary to add coolant into the circulating pump with the vessel until the circulating
pump is filled with the coolant, without overflowing. See the following Figure 1-1:

3
Screw

Figure 1-1

3.4 Annual maintenance

Annual maintenance must be carried out by the company's professional engineers or

qualified personnel trained by the company。
1. Clean up dust attached to various parts of the instrument such as fan, sensor, etc..
2. Check the running condition of the belt of each part, and adjust the tightness of the belt if
necessary.
3. Check the use of instrument piping, replace the aged piping, and replace all piping after
2 years.
4. Check the AD voltage value of the main control board, adjust the channel below 45,000
to 55000, and set OFFSET value is between 20 and 50.
5. Back up the database ABAD files; after the backup, empty all tables beginning with the
letter S in the database, compress and repair them.
6. After two years of use, the bulb needs to be replaced.

4
Chapter 4 Basic Structure and Main Parts of the Instrument

4.1 Main control board

4.1.1 Basic functions

The main control board is the core component of the instrument, which mainly
communicates with the PC to execute various instructions issued by the software. Its basic
functions are to control the work of various moving parts, collect amplification and detection
signals, and carry out AD conversion and send it to the PC for processing and analysis by the
software.

4.1.2 Basic structure

The main chips and interfaces on the main board are the DSP processor, CPLD
programmable logic chip, memory chip, AD conversion chip, AD amplifier chip, RS232
interface, parallel interface of reaction board control, and RS485 parallel bus, as shown in
Figure 1: on the V2.4 main board, OFFSET is near the plugs of each wavelength and GAIN
in the middle; on the V2.0 and V2.2 main boards GAIN is near the plugs of each wavelength
and OFFSET in the middle. 2 rows of potentiometers are close to each other. It is necessary
to pay special attention to the differences between the two versions.

5
 Offset regulator

potentiometer

GAIN regulator

potentiometer lamp

Reset optocoupler

indicator
+12

G
Count optocoupler
G
indicator
-12
图1

± 12V power DSP chip RS232 CPLD RS485 Reaction disc

socket control interface

Figure 1-2

4.2 Motor drive module

4.2.1 Function

Receive the command of the main control board, collect the signal of the sensor, drive and
control the motor and solenoid valve action.

4.2.2 Basic structure

Motor drive module, motor cage, bus plate, common motor board, reaction disc motor board,
5V power board and other components. The instrument has used the bipolar motor and
bipolar motor board, as shown in Figure 1-7.
The instrument is divided into the monopolar motor and bipolar motor with the monopolar
motor board and bipolar motor plate respectively. During their use, special attention must be
paid to: the bipolar motor is 4-wire and the monopolar motor 6-wire.

6
4.2.2.1 Motor Cage
As a bracket mechanical part for installing the motor boards with various functions, the
motor cage is equipped with cooling fans.

Figure 1-3 DS161 Motor Cage

Figure 1-4 DS261Motor Cage

7
Figure 1-5-DS301 Motor Cage

programming
switches from
A2 to A5 are
8421 code
numbers
respectively

图 1-6-DS401 速电机笼

A1 liquid level and

Figure 1-6-DS401 Motor Cage
anti-collision separation switch
ON on A2 is address code +16,
ON=16+ the above code

8
Common motor

board

Figure 1-7
Warning: For all arms, the motor code addresses with different functions may not be replaced
at will! The motor code addresses of the same attribute (such as horizontal) can be exchanged,
and those of different attributes (horizontal and vertical) may not be exchanged, or otherwise
the instrument can not work properly.

4.2.2.2 Bus board

There are the motor board slot, motor line and optical coupling line connector on the bus
board for connecting the motor, sensor and solenoid valve, etc. See Figure 1-8.

Power RS485 Optocoupler Liquid level Motor wire Pump and

interface interface interface interface valve
interface

Figure 1-8

9
4.2.2.3 Common motor board
The common motor board drives the operation of the motors of sample loading arms and
reagent sample trays, as well as the switches of solenoid valves. The address code of this
motor board can be set as required (between 1-15), as shown in Figure 1-9, while the
software setting requires an address code plus 16. Address setting method:
The four rows of pins at the bottom of the MCU chip of the motor board are 8421 codes,
1,2,4,8 counting from the outside to the inside respectively, and can be connected with a
short-circuit plug. The address of the motor board shown in Figure 4 is 6, and the software
setting is 6+16=22
1 2 4 8
ON=0
OFF=1

1 2 4 8 or
Figure 1-9

4.2.2.4 Liquid level integrated motor board

The liquid-level integrated motor board is used to control the operation of the plunger pump
and the switch of the solenoid valve. Figure 1-10 shows the liquid level integrated board.
Liquid level Jumper
line interface code

Figure 1-10

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4.2.2.5 Reaction disc motor plate
The reaction disc motor board is used to control the operation of the reaction disc. There is
no MCU on the motor board directly controlled by the main board through the parallel serial
port. The address code software is set as 0, as shown in Figure 1-11. The motor board A3 is
set as on, version V5.1

Figure 1-11
4.2.2.6 5V power board
The 5V power board is used to power the sensor and motor board modules. If the motor is
not locked after the instrument is reset, please check the working status of the 5V power
board. Bipolar instrument: 5V shall be converted from 24V, and the monopolar motor
instrument is powered by 33V power supply, as shown in Figure 1-12:

11
 Figure 1-12

4.3 Power system

The power system is mainly composed of the power switch, filter, isolating transformer,
switching power supply, wire and other components. The main function of the system is to
provide the working voltage of the instrument, and to control the startup and shutdown of the
instrument. The circuit structure is shown in Figure 1-13:

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 DS301/DS261 circuit diagram 2019.4.23

~220V Input
+5V connect alarm
8A power socket board

Waste alarm board

8A fuse

Water shortage
alarm board
switch

Main
O
I

switch

Cool/on off

Multiple power board

Power switch Power lamp

+-9V 12Vlamp 12VFan 12VFan
220v 220v
Cooling fan
+15V10A +24V12A Heati
Gnd Gnd ng
1 2

Temperature Sensor
Lamp
cool Contro
l
board

Multiple TM4Connect the main

power board board TM4
Red
Cool 1 Cool 2 1 Black 1
2 2
1 2

Fan Black
1 2

1 2

TM2 3 3
TM2 4 Green 4
Fan
1 2

Fan TM6 upward Black Temperature

TM6 upward
Red Red controll
1 2 3 4 5 6

1 2 3 4 5 6
1 2

purple purple meter

Fan orange orange
Black Black
1 2

Fan Black Black

Red Red

Power cool waste water Bus board

DS261 doesn't have that part

Water Sens
Waste Motor
pump or
pump

Pump pump motor sensor

Figure 1-13

13
Power USES :(separated power supply):
Power source
specification 24V 10A 15V 10A
±9V
Motherboard and 24V
Application Refrigeration
temperature +12V 12V Motor and
control board Bulb Fan heating
When the instrument fails to work, it is necessary to check whether the corresponding power
supply is energized and damaged

4.4 Analysis system

The analysis system is the core of the instrument, and the component for chemical reaction
and analysis of reaction absorbance change. All the components work around the analysis
system.

4.4.1 Optical system

The optical system is a post-dispersive system, which is composed of the halogen lamp,
optical fiber and optical filter, etc. The light source used by the filter instrument and grating
instrument is 12V20W.
The structure is shown in Figure 1-14:

Light source Cuvette Optical filter Optical receiver

Figure 1-14

4.4.2 Heating system

The heating system is composed of the heating ring, temperature control board, temperature
sensor, power control board (for use in temperature meter), etc.. The circuit structure is
shown in Figure 1-15:

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 Temperature meter power control board
① 8888 ②

In
Out
8888
⑥
1 2

③
24V or 33V
⑤
Temp. sensor Heating ring
④

Figure 1-15
If the heating ring has no temperature, it is necessary to check：
1 ） Whether the heating ring has resistance of approximately 7.2 ohms, as shown in the
position ④.
2）Whether 24V input power supply at the power conversion board input end is normal, as
shown in the location③.
3）Whether the heating ring terminal has 24V voltage, as shown in the position ④
4）Whether the input IN at the control end of the power control board has 12V voltage, as
shown in the position ②.
If an alarm is sent due to out-of-control heating, it is necessary to check the power control
board, and the chip may be broken.

Temperature control board adjustment instructions

15
 Tens Units
J5 J4 Functionalposition 点位 Point position

J3

J13

S1 S2 S3
J8 J14 J6

Figure1-16

Name Function description

Function P PT100 Channel temperature display: the actual
al temperature of the reaction disc
position S PT100 Channel control temperature setting: the set
（ Press the temperature of the reaction disc (usually 43 ℃)
mode key A PT100 Channel alarm temperature setting: the upper
S1） limit of alarm temperature of the reaction disc（usually
50℃）
F AD590 Channel control temperature setting: the setting
temperature of water heating（usually 45℃）
D AD590 Channel temperature display: the actual
temperature of water heating
Tens The tens place shown by the numerical value

16
 Units The units indicated by the numeric value
Point position The first digit after the decimal point shown in the
numeric value

The functions of three keys S1, S2 and S3 are shown in the table below.
No. Key Function description
1 S1 Mode selection key: switching the functional positions P,
S, A, F and D
2 S2 Decrease button: short press to reduce 0.1; long press to
decrease 1.0 per second
3 S3 Increase key: short press to increase 0.1; long press to
increase 1.0 per second
Interface definition
No. Corresponding interface
J5 AD590 Sensor (water heating temperature monitoring)
J4 PT100 Sensor (heating ring temperature monitoring)
J3 MCU simulation interface
J13 Relay control interface
J8 Temperature control board power supply
J14 Heating ring power supply
J6 33V power input

4.4.3 Detection system

The detection system is mainly composed of the detection board, signal wire and main
control board. The detection board circuit board is shown in Figure 1-17.

List of List of
resistance resistance
340:4.7M 578:510K
405:3M 620:300K
450:1.5M 670:200K
505:510K
图 1-17
546:510K、
The resistance of the detection board for each channel is shown in the table on the right.

17
Note: During the assembly of the detection board, there may be some individual light rays
that cannot be located in the center of the cup, so a few eccentric optical fiber sleeves will be
used for adjustment.

4.4.4 Analysis of the points for attention in system maintenance and

replacement of parts

4.4.4.1 Adjustment of the reaction disk coded disc

1. After adjusting the jackscrew position of the reaction disc coded disc, tighten the coded
disc. The reaction disc coded disc, shaft disc and bearing collar must be pressed in place, and
the shaft disc may not move up and down. See Figure 1-18：

Rotation axis

Jackscrew

Coded disc

Figure 1-18

2. After adjusting the heating ring, be sure to tighten the screw and nut; There shall be no
friction between the heating ring and the reaction disc when the reaction disc is rotated.

18
 Lock screw

Heating ring

PT100 temperature sensor

Reaction Tray

Optical Fibre

Washing head

Figure 1-19

4.4.4.2 Adjustment of optical fiber

1. Adjust one end of the optical fiber copper cylinder to make the spot diameter about
2.5mm.
2. Each strand of optical fiber has a number. No. 1 is used for the detection channel of 340nm,
No. 2 for the detection channel of 405nm, and other strands of fiber can be used for any
detection channel of the remaining wavelength.
3. The M3*3 jackscrew is used to fix the optical fiber head, and the jackscrew cannot be
higher than the surface of the heating ring during fixation, otherwise it will affect the rotation
of the reaction plate.
4. Finally, the fiber is fixed together with a tie band to check whether the rotating shaft fiber
is worn by the rotating shaft disc (as shown in Figure 1-20).

Heating ring
Optical fiber

copper cylinder

crew Optical fibre

bundle

Optical fiber

sleeve
Figure 1-20
5. After installation of the optical fiber, the beam shall be located in the center of the reaction
cup. During debugging, a 5mm wide hard white cardboard strip can be used to observe the

19
light path. If the light spot is too high, the required test dose will be large. Too low light spot
may reach the bottom of the cup, resulting in inaccurate test results.
See Figure 1-21:

Figure 1-21

4.4.4.3 Sensor replacement

The reaction disc has two photocouplers, which are long photocoupler and short
photocoupler. The long photocoupler controls the reset of the reaction disc. If the long
photocoupler fails, the reaction disc cannot be reset. The short photocoupler is count
photocoupler that controls AD sampling. If the short photocoupler fails, the cleaning probe
will collide with the reaction cup during the cleaning of the reaction cup, and the absorbance
detected by the instrument will appear as "0.0000".
1. As for the positional relation between the long and short photocouplers and the coded disc,
the coded disc shall be located in the center of two photocouplers slits, without any friction
with the photocouplers during rotation.
2. For the installation of long and short photocouplers, their supports shall be parallel to the
tangent of the coded disk.
3. After the disc is reset, the two indicator lights of the long and short photocouplers of the
main control board must be on at the same time. If the two indicator lights are not on at the
same time, the detected reaction curve will be serrated, or the cleaning probe will collide
with the reaction disc, as shown in Figure 1-22 and Figure 1-23:

20
 Figure 1-22

Count
photocoupler

Reset
photocoupler

Four-core wire Reaction disc 3M

bipolar motor plug wide belt
Figure 1-23

4.4.4.4 Replacement of optical filters

Optical filters can go moldy in a long-term humid environment, and can also age after a long
period of working, leading to inaccurate absorbance test of the instrument.
1. Remove the damaged optical filter.
2. Install the new optical filter in the filter sleeve, mark the arrow outward, install the
photocell sleeve in the rear, and press the filter tightly to prevent it from loosening. Do not

21
touch the glass surface of the filter directly with tools or hands. Do not wipe the surface of
the filter with water or other organic solution.
3. Install the optical filter sleeve on the heating ring. The filter sleeve shall be level with
the inner surface of the heating ring, otherwise it will affect the rotation of the reaction disc
or damage the reaction cup. Use the hexagonal socket jackscrew to lock tightly, and the
jackscrew cannot be higher than the surface of the heating ring, otherwise it will affect the
rotation of the reaction disc.
As shown in Figure 1-24

Photocell cover Heating ring

Optical filter

Optical filter cover Jackscrew

Figure 1-24
DS261 Install the optical filter of 340 wavelength from the reactor disc 24, and the
optical filter of 405 wavelength from the reactor disc 27, and so on
DS301 Install the optical filter of 340 wavelength from the reactor disc 4, and the
optical filter of 405 wavelength from the reactor disc 7, and so on
DS401 Install the optical filter of 340 wavelength from the reactor disc 90, and the
optical filter of 405 wavelength from the reactor disc 87, analogizing clockwise.
The installation locations of individual instruments may vary, depending on the
specific instrument.

4.5 Sample loading system

4.5.1 Function

The main function of the sample loading system is to automatically add the reagent and
sample required for reaction in the process of reaction of the instrument.
It is composed of the reagent tray, sample plate, sample loading arm, adding sample probe,
diluter, mixing probe, pipeline and other parts.
DS261 The corresponding cup position of the reaction disc for each probe: the
reagent probe corresponding to the cup #1 of the reaction disc, and the mixing
probe also corresponding to the cup #1 of the reaction disc

22
DS301 The corresponding cup position of the reaction disc for each probe: the
reagent probe #1 corresponding to the cup #1 of the reaction disc, the reagent probe
#2 corresponding to the cup #76 of the reaction disc, and the mixing probe
corresponding to the cup #74 of the reaction disc
DS401 The corresponding cup position of the reaction disc for each probe: the
reagent probe #1 corresponding to the cup #1 of the reaction disc, the sample probe
corresponding to the cup #86 of the reaction disc, the reagent probe #2
corresponding to the cup #76 of the reaction disc, the mixing probe #1
corresponding to the cups #40 & #41 of the reaction disc, and the mixing probe #2
corresponding to the cups #30 & #31 of the reaction disc

4.5.2 Installation methods of reagent and sample trays

4.5.2.1 Replacement of motors

The motor is a stable component that is generally not damaged.

1. Loosen the four screws of the motor support to make the belt in a relaxed state and
remove the damaged motor from the motor support.
2. Install the belt on the synchronous belt pulley and fix the screws at the four corners of the
motor.
3. Tighten the belt and tighten the four screws of the motor support.
4. Adjust the synchronous belt pulley so that the belt has no friction with the coded disc and
the synchronous belt pulley, and tighten the jackscrew of the synchronous belt pulley.
See Figure 1-25：

Motor retaining screw Support retaining screw

Motor Synchronous belt pulley Belt

Figure 1-25
How to determine the motor fault or motor board fault: The good control board circuit can be
used to control a faulty circuit. If there is any abnormal phenomenon, it can be judged that

23
the motor is faulty; conversely, it can also be judged that the control board is faulty.

4.5.2.2 Belt adjustment and replacement

The belt will loosen and age after working for a long time. The tightness shall be adjusted or
a new belt shall be replaced.
1. Adjust the tightness of the belt, loosen the four screws of the motor support, then retighten
the belt, and fix the four screws of the motor support.
2. To replace the belt, loosen the four screws of the motor support, remove the photocoupler
of the coded disc, and remove the aging or damaged belt.
3. Replace into the new belt and tighten it, and fix the four screws of the motor support to
restore the photocoupler.
4. Check whether there is any belt friction with the coded disc or belt deviation.
See Figure 1-26：

Figure 1-26

4.5.2.3 Replacement of cooling sheets

1. Remove the damaged cooling sheet.

2. Uniformly coat the contact surface of the reagent tray, the surface of the cooling sheet and
the surface of the radiator with the heat-conducting silicone grease.
3. Attach the cooling surface of the cooling sheet to the reagent tray metal disc, and the
cooling plate to the heating surface, and finally install the radiator.
4. Electrify and cover the cooling sheet to test its cooling effect. After 10 minutes the
temperature of the cooling plate shall be obviously decreased by more than 10 ℃.
See Figure 1-26：
Method for distinguishing hot and cold surfaces of cooling sheets：
The black line is on the left, and the upper end of the cooling sheet is the cooling surface, or
it can be electrified briefly. Touch with your hands to separate hot and cold surfaces.

24
Water block

Cooling pad for pan

Metal disc cooling

Figure 1-27

4.6 Liquid road system

4.6.1The function of the liquid road system

The function of the liquid road system is to automatically clean the adding sample probe
during the sample loading process of the instrument and to automatically clean the reaction
cup after the reaction analysis of the instrument is completed.

4.6.2 The structure of the liquid road system

The liquid road system mainly consists of the feed pump, liquid waste pump, three-way
solenoid valve, two-way solenoid valve, cleaning tank and cleaning head as well as other
parts. See Figure 1-28 for the piping diagram.
In the process of pipeline connection, the pipeline shall be connected naturally without the
following mistakes:
1. The pipe is bent 90 degrees.
2. There are squashed pipelines.
3. The liquid waste pipe of the cleaning pool is U-shaped.
4. The excessively long pipeline is bent in the enclosure.
5. The pipeline and fittings are of different sizes.

25
 water
Drain the waste liquid
One way
Φ5 ID V1-6 Valve
IN Muti-Valve
B2

vacuum bottle
Water pump
B1
pump

Water Φ5
V8 NO Valve air
heating
washer Waste B
Φ5 ID
Air F3
Mixer
2 way value
NC
NO
M1 R1 R2
F 3 way value
Air bottle
6 way

A B C F1 F2

S1 S2
Waste
C
R1 R2
Water sensor Waster sensor
S1 S2 A water
B high contraction waste
A B C C low contraction waste
water waste waste
Description: the 300 speed instrument has 2 syringes R1&R2;
The 200-speed instrument has only one syringe R1

Figure 1-28

26
Chapter 5 Correction and Replacement of Vulnerable Parts

In order to ensure the normal and reliable operation of the instrument, timely and effective
maintenance of the instrument and correction & replacement of some parts are essential.

Note:
The following maintenance and correction & replacement of some parts can be completed by
users themselves only after having successfully passed the training by professional engineers.

5.1 Replacement of bulbs

After the bulb has been confirmed to be damaged or has been in use for two years, it needs to
be replaced. The steps for replacement are as follows：
 Power off the instrument and wait for 15 minutes for the bulb to cool；
 Open the back plate of the instrument；
 Loosen the bulb support nut
 Replace it with the new bulb and tighten the bulb support nut as shown in Figure
1-29.

Bulb socket Lamp holder

Figure 1-29

27
5.2 Replacement of probes
The method for replacing the adding sample probe is the same as that for replacing the
reagent probe, and the specific steps are as follows：
1. First, open the top cover of the probe and use pliers to cut the tie wire fixing the
probe；
2. Screw off the screw fixing the thread as well as the forcing screw fixing the probe；
3. Remove the adding sample probe and remove the hose that covers the adding sample
probe；
4. Replace into the new adding sample probe and put on the hose. The hose structure is
shown in Figure 1-30 and Figure 1-31 below：
5. Use the forcing screw to fix the probe and thread, and then use the tie wire to fix
them, making sure they are vertical and the spring can freely bounce, and finally cover them.

Figure 1-30

Probe signal Liquid level line interface P1 and P2

interface

Figure 1-31

28
5.3 Replacement of reaction cups

If the reaction cup is contaminated or appears to be worn, the blank absorbance value of the
reaction cup may be higher than 0.02a during the blank test. If the absorbance cannot be
improved after cleaning, it is recommended to replace the cup.
Note：
1. The front and back sides of the reaction cup are the test surfaces. Do not touch the test
surface when placing the reaction cup.
2. Ensure the upper surface of the reaction cup placed is flush, otherwise it will easily cause
residual water when cleaning the reaction cup, thus affecting the accuracy of the results.
3. Use the same batch of reaction cups as much as possible

5.4 Replacement of power fuses

The specific steps for replacing the power fuse are as follows：
1. Take out the fuses from the accessory bag of the instrument. 8A fuse is used in the main
machine of the instrument and 4A fuse is used in the water heating system. Do not use the
wrong fuse.
2. Turn off the instrument power and remove the power plug.
3. Unplug the power cord from the power outlet of the main machine and extract the fuse
holder from under the power outlet (tools can be used).
4. Remove the damaged fuse, put the new one into the fuse holder, and plug the fuse holder
back into the power socket.
5. Plug in the power plug.

5.5 Adjustment of the signal value GAIN and background value OFFSET

5.5.1 Adjustment of the background value OFFSET

In general, if the reaction curve is bad, the absorbance of the reagent is abnormal or very high,
above 2.2, or the repeatability is bad, the OFFSET value needs to be checked.
1. After the online instrument is reset, enter the "Cup blank detection " interface of the
software and select "Real-time detection". The interface is shown in Figure 1-32：
2. Take out the reaction cup at the position of each wavelength and put in the black light
blocking cup. See Figure 1-33：
3. Observe the real-time voltage value, and adjust the potentiometers in each channel in the

29
right column of the main board to reach the required value (20-50).

Figure 1-32

Figure 1-33

30
5.5.2 Adjustment of the signal value GAIN
Gain value is adjusted as follows：
1. After the instrument is reset, enter the "Cup blank detection " interface of the software and
select "Real-time detection".
2. Click water injection and wait for the completion of water injection.
3. Observe the real-time voltage, and adjust the potentiometers in each channel in the left
column of the main board (see Figure 1-1 for details), to make the voltage value reach about
54000.
Chapter 6 Common Faults and Troubleshooting
Fault phenomena Possible causes
1）Connection failure of power
1. No action after cord.
the power switch
2 ） The main program is not
started.
is turned on (the
3）Fuse breakage.
power indicator is 4 ） The AC socket has no
off) electricity.
1 ） The correct COM port
MAINCOM=0（COM1）is not
selected for the instrument .
2 ） The connection of RS232
data line or the internal data
line is poor.
2. The computer
3）Detection channel settings of
fails to connect the instrument are incorrect.
with the 4 ） The COM port of the
computer is damaged.
instrument or the
5 ） Whether the motor control
instrument does signal wire falls off.
not work (the 6 ） The main program is not
started or there is something
power indicator is
wrong with the motherboard.
on)
7 ） The hardware parameter
"Hardware" of the instrument is
lost.
8 ） The software runs two
windows.
9）Abnormal power board or 24
31
Troubleshooting steps
1）Check the power cord connection.
2 ） Restart the device five minutes after
shutdown.
3）Check the fuse.
4）Check whether the socket has electricity.
If the problem still exists, please contact
CLINDIAG's after-sales service department
or the designated local maintenance agency.
1）Reset the COM1 port of the instrument.
2）Check the data cable and re-plug it.
3）In "Biochemical analyzer running parameters \
equipment", recheck the setting of detection
channel, and select 12\8 channel. The motherboard
shall be checked in 12 channel.
4）First, use the "Serial assistant software tool" to
check whether the serial port is in normal
communication. The serial port 2 and 3 pins are
short connected. In the event of any abnormal
phenomenon, replace the COM port of the
computer.
5）Check the motor control line, and whether the 6-
core control line is loose.
6 ） When the software isn’t removed from the
memory, restart after shutdown for five minutes.
Keep offline to consider there is something wrong
with the motherboard.
7）Copy the backup file or use "System hardware
parameter recovery" to restore the password "888".
(Back up as hardwarebak upon installation)
 switching power supply 8）Close one of the windows.
9 ） Measure the output of power board and 24
switching power supply and replace it in case of
abnormality.

1) The pipe is damaged. Turn off the power, wipe dry the leaked liquid, and
check whether the joint falls off, whether the pipe
2) The joint falls off.
3. Liquid leaking is damaged, whether the pump membrane of waste
3) The diluter leaks. liquid pump is damaged, or whether the bubble box
from the
4) The three-way valve is leaks, and check the joints.
instrument
blocked.
5) The pump leaks.
6）The bubble release box leaks.
1）The adding sample probe is 1）Use an acupuncture needle to pierce through the
blocked. probe, and implement “Probe maintenance”.
2）The diluter does not move. 2 ） Check the diluter and motor, as well as the
3）The diluter leaks. diluter address code settings, and check the
4）The sample probe is inserted connection.
into the bottom of the cup. 3）Replace the diluter piston.
4. The sample 5 ） The sample probe has a 4）Adjust the sample probe height..
cannot be sucked problem in liquid level 5）Lower down the liquid level detection sensitivity
sensing, and it does not go and check the connection. See "5 Treatment
up
down. Methods for Liquid Level Problems " for details.
6 ） Pipe broken or joint falling 6）Check whether the joint falls off or whether the
off or bent. pipeline is broken or bent.
7）The corresponding solenoid 7 ） Test the solenoid valve with the action test
valve is broken or out of program. The measured voltage is 12V.
control.
1 ） The line of liquid level 1）Check the level sensor line. Reconnect the plug
5. The sample sensor is disconnected, or the for reinforcement, and check the connection
contact is bad. between the liquid level plate and the motor board;
probe is inserted
2 ） The level indicator light Check the liquid level anti-collision baffle to make
into the bottom of flashes in a static state, and the sure it is at the bottom of the photocoupler.
the cup or does sensitivity of the liquid level 2)
detection board is too high or 1.Check that the voltage between the ground wire
not go down
too low. Circumferential wire and the zero line of the instrument’s power supply
(liquid level
interference, and poor ground shall be equal to or less than 3V; pay special
problem) line. liquid level curve attention to the connection of the ground wires
[Interior wire with fluctuation is very big, using the regulated power supply and the UPS
exceeding 100, and water power supply (for unqualified ground wires, a
a serial port and
quality is bad, or a cleaning metal pile can be driven outside and connected to
serial port line are agent was misused. the bottom plate of the instrument)；
required] 3 ） Incorrect setting of probe 2. One of the holes of the liquid level detection
descending height parameters. board is the ground terminal, and the liquid level

32
4）The motor control board, or
liquid level plate is broken.
5 ） The sample cup cannot be
placed at the bottom of the
bracket due to its deformation.
6 ） There is reagent residue on
the outer surface of the reagent
bottle, and the reagent probe is
inserted into the bottom of the
bottle.
33
detection board also needs to be grounded；
3. It is required to check the reagent tray metal
locating pin by grounding and to connect the pot to
the bottom plate；
4. The flexible flat cable on the beam arm needs to
be fixed and to avoid abnormal signal interference,
the red and black lines of the probe are fixed; the
flexible flat cable shall also be fixed at the rear end
and connected reliably；
5. Comb the flexible flat cable, which shall be
sheathed with heat shrinkable tube, separated from
pump line, power cord, motor line and metal, and
shall not touch the metal base plate during
movement；
6. Sensitivity adjustment: Connect the liquid level
tool software, observe the static level curve, and
write the new liquid level value 25 (18-25). The
smaller the value is, the more sensitive the liquid
level will be. The default value is 18 and the liquid
level tool V1.0, as long as the sample 300UL is
detected. The 2-in-1 liquid level plate is generally
set with the sample 160 and test tube 200. If the
interference is large, a larger value (sample 200,
test tube 300) can be selected, with the liquid level
tool V1.1.0-V1.1.4. The sample liquid level value
of the 800 velocimeter and the reagent is 180 and
300 respectively, with the liquid level tool V1.1；
7. Low-sensitivity probes will be inserted into the
bottom of the reagent bottle. Reduce the liquid
level threshold value and improve the sensitivity；
8. Water may not enter the inside of the probe,
otherwise short circuit will be caused and
replacement must be made.
9. If the liquid level curve fluctuates more than 100,
do not add alkaline cleaning solution to the water,
measure the conductivity of pure water, or connect
SBD and NCL pipelines inversely.
If the reagent tray surface is not connected with the
instrument bottom plate, the screw hole can be
repaired or the bearing can be replaced to ensure
that the locating pin point is connected with the
instrument bottom plate.
3）Adjust the descending position of the probe. The
reagent probe shall be 3mm away from the bottom

1 ） Pipeline damage or falling
off, causing air leakage.
2）Reagent probe is blocked or
slightly blocked.
3）The corresponding solenoid
6. There is water valve is damaged or has
impurities.
hanging at the tip
4）The diluter leaks fluid.
of the probe after 5）The sample probe is inserted
cleaning into the bottom of the cup.
6 ） There are bubbles in the
pipeline or in the plunger pump.
1 ） After cleaning, the short
7. Dripping,
probe drips, and the one-way
splashing or no valve is not closed tightly.
water from the 2 ） After cleaning, the long
cleaning probe probe drips, the descending
depth of the probe is too deep,
(the cleaning
and the tip touches the bottom
block touches the of the cup.
34
of the reaction cup, and the sample probe shall be
3mm away from the bottom of the reaction cup and
the bottom of the serum cup. At the same time, the
depth shall be set to reach the rim of the cup for
volume calculation.
4) Test the motor board with the action test
program. If the level does not respond, replace or
exchange it.
5）Select qualified sample cups.
6）Wipe the outer surface of the reagent bottle and
the reagent tray to make sure there is no reagent
residue, or clean the reagent tray regularly.
7）If the ground wire is not good, the bottom plate
of the instrument can be connected to the ground
wire end of the wall socket.
8）V3.72 motor board, SV3.24 program. Only the
test tube can be used to write the liquid level value
with V1.1.4 tool, and 260 is recommended. The
serum cup to be added in the later period can be
used to write the liquid level value, and 160-200 is
recommended.
1）Inspect the piping and fittings.
2 ） Clean with “Probe maintenance” or use an
acupuncture needle to pierce through the probe, and
open the pump and valve with the motor program
to pump water that shall be flowed out in a straight
line。
3） Feed water to switch on/off the solenoid valve
for test with the action test program; open the
solenoid valve to clear the foreign body.
4）Replace the diluter piston or glass tube and add
grease.
5）Adjust the height of sample probe.
6）NCL cleaner can be used to clean the pipeline,
and the probe shall be cleaned 5 times. It is
recommended to add NCL cleaner into the water.
7）Unplug the pipeline, clean it first, then use the
syringe to exert reverse pressure to make the
one-way valve closed completely. Solenoid valves
cannot be tightly closed, which may be due to the
entry of foreign matter.
8 ） Adjust the descending depth so that the long
probe cleaning block just touches the bottom of the
 cup) 3 ） The washing head is long, cup.
the short probe drips, 7 long
9 ） Adjust the long probe to the same horizontal
probes are uneven in length at
line. The cleaning block shall be 1mm lower than
the bottom, and inserted into
the 7 long probes, and the maximum number of
the bottom of the cup, and
water absorption is not steps shall be lowered. The cleaning block shall
complete, resulting in back touch the bottom of the cup, and other probes may
leaking. not touch the bottom of the cup.
4）When the instrument is reset 10 ） The three-way solenoid valve might not be
or the sample probe is cleaned,
tightly closed. Open the solenoid valve to clean
the short probe of the cleaning
valves.
head drips.
5 ） No water flows from the 11）The plug of the solenoid valve is loose or bad,
short probe. or the valve driver plate bad, or the control end
6）Loose cleaning block. without voltage, or the wiring or motor plate faulty.
7）The location of the cleaning 12）Fix the cleaning block with glue.
block is not centered, touching
13）Adjust the cleaning block to make it vertically
the cup.
centered, or adjust the position of the cleaning head
8 ） The water of the cleaning
or bracket, so that the cleaning block is in the
agent probe of the cleaning
head splashes. center of the reaction cup. Also pay attention to the
belt tightness, and check whether the belt is
damaged.
14）Use the pump voltage adjusting plate to adjust
voltage to 20V or lower. The software can be set to
reset after upgrading for 2 times.
1）Dirty reaction cup. 1）Replace the reaction cup。
2）Light path spot is not located 2）Adjust the reaction disc to center the light spot,
in the center of the colorimetric and the distance between the spot and the bottom of
cup. the cup shall be about 1.5-2mm.
3 ） The reaction cup voltage is 3 ） Readjust the signal value 55000 and the
not in the normal range. background value 20.
4 ） Pipeline broken, bent and 4）Check the pipeline to avoid bending and leakage.
falling off. 5）Replace the glass tube or piston and add sealing
8.Inaccurate 5）Water leakage in the diluter. grease.
results 6 ） Improper adding sample 6 ） Adjust the position and height of the adding
probe location or height. sample probe. The probe may not be inserted into
7 ） The reagent or control the bottom of cup.
material is invalid. 7）Replace reagents and control materials.
8）The solenoid valve damaged. 8 ） Test solenoid valves with the action test
9）Liquid level detection failure. program.
10 ） The detection parameter 9）Adjust the sensitivity of the liquid level detection
board.
setting is wrong.
10）Check and reset the parameters.

35
11 ） Detection voltage and its 11 ） Check grounding and voltage stabilization;
Check whether the detection board is loose and
absorbance are unstable.
whether the optical filter is damp; check whether
12）The sample suction probe is the detection board is installed perpendicular to the
blocked. light path, whether there is light leakage, whether
the two ends of the optical fiber are fixed and
13）The temperature control of
tightened, and whether the light spot is between φ2
the reaction plate is not and φ2.5.
accurate.. 12 ） Clean with Probe maintenance or use an
14）RS232 data cable is loose. acupuncture needle to pierce through the probe.
15）There is water residue in the 13）Recheck or adjust the reaction disc temperature
reaction cup because the control.

reaction cup is not placed 14）Fix the data cable.

15）Adjust the position of the reaction cup and the
evenly or the cleaning head
height of the cleaning head to ensure they are
height is not suitable.
horizontal.
16）The coded disc rubs against
16）Readjust the coded disc to center it, and clean
the photocoupler. The belt is
the photocoupler with alcohol or replace it; test the
damaged.
reaction disk belt MXL250 for damage, and replace
17 ） Poor contact of test line.
it；
The motherboard is not reliably
17 ） Reconnect the test wire and check the
grounded.
grounding wire；
18）The unused test channel is
18）Use jumper blocks to short-circuit P2, 3 pins on
not short-circuited.
the motherboard.
19） The count photocoupler is
19）Reinforce the count photocoupler.
loose.
20）Increase the pump water feeding time, and the
20 ） Insufficient water volume
amount of water shall be 1/2-2/3 of the cup.
for cup cleaning.
21）Replace bulbs.
21）The light bulb aging
22）2*4 times of probe cleaning can be performed,
22） The bubble release box of
and 3*4 times of probe cleaning performed for the
the adding sample probe is
first installation before full flooded operation.
short of water, resulting in error
23）The post-pollution and pre-pollution items can
in sample suction.
be designed, or the optimization setting can be
23）Serious cross contamination
started, and the excessive amount of reagents won’t
24）Abnormal cup blank
be extracted. Also note the test sequencing between
items.
24）Make sure to pass the cup blank test, and the

36

1）The light bulb is damaged.
2）The bulb cable is loose.
3 ） There is something wrong
9. The voltage with the bulb power supply.
4 ） There's something wrong
value is 0 or very
with the signal cable.
low for blank 5 ） There's something wrong
detection with the motherboard.
6）The optical filter is damp.
7 ） There is something wrong
with the RS232 cable.
1）The sample suction probe is
blocked.
2）Water leakage in the diluter.
3）Liquid level detection failure.
10. Reagents and
4）The corresponding solenoid
water cannot be
valve is damaged.
absorbed and 5）Detached pipeline.
delivered 6）Pipeline breakage.
11. The set Hardware file properties are
mechanical read-only
parameters cannot
be saved
1 ） “blankVotage” in
Hardware file is not set.
12. All red prompt 2 ） The Cupblank file has an
during blank unusual character or is saved as blankVotageMax=62000
detection 0 blankVotageMin=30000“.
1）The red prompt indicates that 1）If the detection voltage is lower than 30000, the
13. Blue and red
water volume of the cup shall be up to 60%.
1）Replace the bulbs.
2）Check the bulb cable.
3）Check or replace the power supply.
4）Check the signal cable.
5）Replace the motherboard.
6）Replace the optical filter.
7） Check the reliability of the cable or replace it.
Real-time detection voltage can be used to check
whether the signal is echoed
1 ） Clean with Probe maintenance or use an
acupuncture needle to pierce through the probe.
2）Replace the glass tube or piston.
3）Adjust the sensitivity of the liquid level detection
board. (Others the same as above.)
4 ） Test the solenoid valve with the action test
program.
5）Check the pipeline.
6 ） Detect and handle broken pipelines under the
sample loading arm.
Remove Hardware Read Only Attribute.
Modification of this setting can also be avoided.
the 1 ） In the Hardware file, follow the settings as
shown below：
“blankODFilter=0.025
2）You can empty the contents of the Cupblank file
and retest it.
37
 the normal detection voltage reaction cup shall be replaced; if it is higher than
prompts during
range 30000—62000 is 62000, the detection voltage shall be adjusted.
exceeded. 2）Cups need to be re-cleaned or replaced with cups
blank detection
2 ） The blue prompt indicates with a small difference.
that the range of blank
absorbance is exceeded, with a
big difference between the
cups.
1）Wavelength setting error. 1）Reselect the correct wavelength.
14. Wrong 2）Wavelength installation error 2 ） Check the correct installation position of each
3 ） Cupblank has confusing wavelength.
absorbance characters that affect the results 3） Open this file to empty its contents, conduct a
of each wavelength. cup blank test again, and save.
4 ） Insufficient test dose and 4）Pipeline breakage
serum

1 ） The database has extra 1）Database compression and repair

characters 2）Install Southeast Asian languages
15. The software 2 ） Southeast Asian languages 3 ） Pay attention to database matching when
not installed upgrading software.
cannot work or 3）Database mismatch 4）Take care to check whether the executable file is
4）Executable file missing infected by the virus. If infected, remove the virus
reports an error
5）Windows 7 system reason by anti-virus software.
5）The software can be run as administrator；
1 ） The Hardware file is set 1）Check the hardware settings, and find the file.
16. Unable to
incorrectly, or lost.

enter hardware

parameter settings

or working

improperly
1) Photocoupler problem 1) Check the photocoupler connection line, clean
17. Abnormal 2) Abnormal motor plate up the photocoupler dust and slit dust, or
3) Collision avoidance replace. You can use a multimeter to test the
movement of the response yellow line, as well as 5V and 0.7V changes.
4) The reaction disc often 2) Check the motor connection, especially the
instrument parts,
rotates plug or replace the motor board. When the
injection, e.g. the 5) The cleaning arm isn’t motor board problem is determined, other
lifted before rotation motor boards can be used to interchangeably
reaction disc often 6) Bad connection of wire open and control the motor to determine
plug whether it is the motor board problem or the
rotating or
7) The reaction disk is in motor problem. Check the address code

38
 abnormal rotation, and 3)
abnormal sound,
cannot be rotated in place;
the buzzer control address 4)
the cleaning arm
is wrong
isn’t lifted before 8) The correction of the
serum plate cup level is
rotation, and the selected, but set to 0 5)
reagent tray and
sample disk are in
abnormal rotation.
1) The linear setting is not
accurate, and the result is
greater than the linear
value, resulting in dilution
2) Standard position
misplaced or uncorrected
3) The absorbance value of
the auxiliary detection
18. Repeated
point is greater than the
dilution by the linear value, resulting in
dilution；
instrument 4) The straightness cor of the
curve is less than the set
value 0.65, and the curve
fluctuates greatly；
5) Test to find that
absorbance is beyond the
multi-standard absorbance
range, resulting in dilution
The database table is open
19. The new table
but not closed
cannot be opened
39
Adjust the probe baffle and spring, and make
them drop to the bottom of the photocoupler.
Check the connection line of the photocoupler,
check the slot of the bus board, and check
whether the shrapnel is out of shape or
short-circuited.
The same as 4) above. In addition, water may
enter into the cleaning arm motor, resulting in
abnormal operation.
6) Check the motor and optocoupler connection
line, and separate the optocoupler connection
line
7) Setting the address of opening the buzzer to 0
will lead to the conflict with the reaction disc,
and the disc will stop when the buzzer is
started, leading to the second sample loading
in the cup.
8) The correction of the serum plate cup level is
selected, and it is not allowed to set to 0.
Generally, it is set to 5 (the numbers of 5-20
are ok), that is, for every 5 specimens added,
the disc is reset once.
1) Accurately set linearity
2) Take care to check the standard, and make
accurate positioning
3) You can expand the linear range, which won’t
result in dilution.
4) Check the stability of the instrument, such as
bulb, cup, sufficient test dose, clean cup, etc.,
to ensure smooth reaction curve.
5) Test whether the calibration curve is a positive
reaction, and the correction coefficient is set
as 1, not 0.5 or 0
1）Use the software dated June 27, 2016
 1) The computer is not 1) It is recommended to replace the computer or
20. Tip: This
configured properly reinstall the system
2) The database is too big 2) Database compression repair. You can also
memory cannot
back up the data first, then delete the data, and
be read-only re-compress repair
1) Very low reaction 1) Note the effectiveness of the reagent, and the
21. Reagent
absorbance reagent can be replaced
2) Abnormal parameters 2) Adjust parameters
problem
1) Hardware parameter 1) Find the parameters
22. MFC error;
setting exception 2) Empty the database, compress and repair it,
2) Illegal characters in and find the database
insufficient
database, and incomplete 3) Reinstall the system
parameters, database fields
3) System instability
expected to be 1,

2
1) English environment 1) Remove the contents of Maintain and redo the
23. Prompting
cannot recognize morning blank test
and afternoon
blank test again

within 2 hours

after cup blank

test
1) Database missing fields, 1) Replace the database
not being this version 2) Pay attention to the elimination of system
24. The database
2) Virus software files viruses
cannot be opened deleted

1) Hardware parameter 1) Find the parameters

25. Data type setting exception 2) Empty the database, compress and repair it,
2) Illegal characters in and find the database
mismatch in the
database, incomplete 3) Reinstall the system
standard database fields
3) System instability
expression
1) Cleaning agent probe 1) Use the pump to control the electric pressure
26. Cleaning head spraying water, big pump plate, and adjust the pressure to about 20V
pressure 2) Before each valve of the cleaning head, add a
probe spraying 2) Water injection probe thin pipe to release water pressure. This
spraying water pipeline can be connected to the cleaning cup
water, causing
or waste liquid container, as shown in the pipe

40
 drawing
splashing
1) When a computer is 1) Cancel the wireless network card, or fix the IP
27. The
connected to a wireless address
network card, the IP
authorized
address will change
software prompts

invalid
During sample loading, the 1） In hardware [device], the alarm will be
28. The software descending depth of the cancelled at armAlarm=0, but the location
probe at the reagent tray is error message is written to a strike.txt file
prompts "wrong less than the parameter 2） Liquid level is too sensitive or the
setting of "the highest anti-collision baffle is bounced
probe location "
designed liquid level"
The bipolar anticollision The anti-collision baffle is bounced.
29. Software
separation motor board Note that the probe shall slide freely up and down.
receives the anticollision The baffle is in the middle of the photocoupler.
prompts “ arm
signal
collision”'
1 ） N/A shown before the test 1）Wait for complete reaction
results are available 2 ） Check the liquid level and add the reagent or
2）Missing reagents or samples sample
by alarm 3 ） Check whether the reaction curve is linear;
30. Test results 3 ） Rate method. Linear cor=0.65 condition has been relatively loose.
difference in reaction 4 ） Check that all points on the multi-standard
display “N/A”
curve is less than cor=0.65 calibration curve are positive reactions, and the
in the hardware setting correction coefficient shall be set as 1, not 0 or 0.5
4 ） Abnormal multi-standard
calibration curve
1）The difference of absorbance Pay attention to the selection of standard and the
between two points is less than dilution ratio. Absorbance can't be too close to the
0.05 correction factor, and the default value shall be
2）The slope is less than 0.1 changed to 1
31. Multi-point 3 ） The first turning point is
premature
calibration 4）The absorbance of the point
behind the inflection point of
"calibration curve
the rising curve multiplied by
the "multi-standard correction
is not legal"
coefficient" is less than the
standard absorbance of the
minimum concentration
5）The absorbance of the point

41
 behind the inflection point of
the descending curve multiplied
by the multi-standard correction
coefficient is greater than the
standard absorbance of the
maximum concentration
6）The curve has several turning
points
1）Empty the ACTION_COMB Chinese characters are not generally recognized in
32. Combined
table in the database and add it English system. Therefore, pay attention to the
again deletion of characters.
action name in a
2）Arrow font in language pack
state of random 3 ） Delete the full name of the
item parameter in the database
code 4）Font exception. Copy all font
files under the Windows
Sample results
directory in c disk.
"prompt" random

code

The "full name"

of sample results

in a state of

random code

1 ） There is something wrong 1）Reinstall the computer system

with the computer system 2）Set the computer time format to year month day
2）The computer time format is without week
provided with a week 3 ） Use the antivirus software to kill viruses. For
3）The software is infected by a security, during installation and after the
virus completion of debugging, the software shall be
33. The software 4）Database exception backed up to the D disk in a compressed format,
5）Hardware missing and be avoided from being infected by the virus in
fails to work
use.
4 ） Databases shall be backed up, emptied,
compressed or repaired, or the backup database
shall be used.
5）Use the backup hardware, and try to avoid power
outage.
34. Prompting 1）maxAbs=2.5 1） The maximum absorbance of some reagents is

42
that the 2）maxFactor=15000 very high, and can be changed to 2.8
2）If the factor is too large, it should be considered
absorbance is out
that the reaction is too weak and the absorbance
of range, or the change is too small, which will cause the result to
calibration factor be unstable. The cause shall be detected.

is out of range
35. Filter=0 or None or causing the Versions V3.86.17.10.21 and V3.86.18.3.29 have
DI800
wavelength in the item already supported selecting 0 or None
absorbance error,
parameter to automatically shift
filter position forward by one
error
1 ） Installing electrolyte will 1）Check if the electrolyte installation is set
lead to normality 2 ） In the location of note & name entry a heavy
36. Reporting
2）Illegal or more than 50 space weight is placed on the keyboard, resulting in a
"electrolyte characters are available in the countless number of space entries. Open the
database database, find the record of reporting an error, test
pipeline cleaning" all patient information bars, and delete overlong
spaces

43
Appendix 1: Wiring Diagram of FA Series Instruments

1. Wiring Diagram of DS401 Motor Drive System

44
2. Wiring Diagram of DS301 Motor Drive System

3.Wiring Diagram of DS261 Motor Drive System

45
Appendix II. Maintenance Manual for Electrolytes

1. Fundamentals of the Electrolyte Analysis Module

The electrolyte analysis module is developed on the basis of the potential measurement of
ionic electrodes and reference electrodes. In an electrolyte, most salts are broken down into
ions. This electrical exchange reaction occurs mainly between the relevant electrode and the
ion, and thus forms an electrode potential difference between the ion selective electrode and
the reference electrode. This potential difference provides a continuous potential, and the
corresponding ion concentration can be measured by measuring the electrode potential
difference.

The potential difference of the electrode can be obtained from the theoretical formula of
Nernst:

2.303RT
E=E。+ Nf logaxfx

Wherein：

E：The potential of ion selective electrodes in the measurement solution

E。：The standard electrode potential of ion-selective electrodes

N: The charge number of measured ions

R: Gas constant

T: Absolute temperature

f: Faraday constant

aｘ：Measured ion activity

fｘ：The activity coefficient of measured ions

2. Maintenance of the Electrolyte Analysis Module

2.1 Daily maintenance

a) Check the calibration solution A and B to ensure that the amount of the calibration
solution A and B is sufficient.

46
2.2 Semiannual maintenance

a) Replace the pump pipe.

b) If the electrode is filled with less than 2/3 liquid, please replenish the liquid.

c) Check the electrode potential. Its voltage shall be more than 50 millivolts, otherwise

its shall be replaced.

3. Main Parts

3.1. Main control board

3.1.1 Function
The main control board can be divided into: power supply module, detection signal
processing module, serial port module, motor drive module, solenoid valve control module,
etc. by function.
3.1.2 Basic structure(see F2.1)

F2.1
3.2.1 Functions
The detection board mainly realizes data acquisition, amplification, transmission and other
functions. The signals generated by the electrode are collected and amplified by the detection
board to the 10th – 12th power times of 10 before being transmitted to the main control board
for data processing.

47
For the pipeline flow chart, see f2.3

F2.3
3.3 Assembly
3.3.1 Function
The assembly includes the main mechanical structure parts of the instrument: electrode
assembly part, detection board assembly, peristaltic pump part, solenoid valve and pipeline
part.
3.3.2 For the basic structure, see F2.4

48
 F2.4
3.3.3 For the interior wiring sequence of serial ports, refer to F2.5

49
 F2.5

4. Setting of Electrolyte Parameters

When we debug the electrolyte, we usually use the underlying hardware debugging tool of
the electrolyte for debugging. After the electrolyte is debugged by the debugging tool to be
up to grade, the biochemical software shall be opened for online debugging.
Open the electrolyte debugging tool interface as shown in F2.6 below:

F2.6
The steps for using the electrolyte debugging tool are as follows：

Click on to open the electrolyte debugging tool software. The user name is

50
Admin, and the password is also Admin login.
Click on the menu to switch to Chinese. Select the correct serial port in the serial port
setting, select the baud rate of 9600, and then click the button to open the serial port. At this
time, the working status scrolls through the instrument being ready.
Enter the Standard A concentration A, Standard B concentration, K correct value and d
correct value in the parameter setting area, and tick the K, Na, Cl, Ca and PH channels to
indicate that these channels are valid. The screenshot is shown as f2.7 below:

F2.7
1) Click the "Read Para" button to show all parameters. Enter the Standard A concentration
A, Standard B concentration, K correct value and d correct value in the parameter setting
area, and tick the K, Na, Cl, Ca and PH channels to indicate that these channels are valid.
2) Reagents (Standard A and Standard B) sampling volume setting: first set the Standard A
and Standard B, sample, reagent, air gap and initial empty value to 450, 450, 625, 650, 1200,
2000 respectively, and then click the sampling test behind the Standard A, observing whether
the sample cup has been filled with the liquid A. If not, the sampling volume of the Standard
A shall be increased; in case of excessive overflow, the sampling volume of the Standard A
shall be decreased. The sampling volume setting of the Standard B is the same as that of the
Standard A.
3) "Reagent" setting: after the sample cup is filled with the Standard A, click the sampling
test button of the "reagent", and then the electrode is put into the Standard A to ensure that
there is liquid on both sides of the electrode and the liquid level sensor lamp is always on (the
liquid stays at L2). The reserved length of liquid L1 in the liquid inlet end pipeline is about
4cm.
4) "Sample" setting: inject 200ul solution into the electrolyte cup by the sampling gun, and

51
then click “sampling test”, observing a slight margin at both ends of the electrode. Note: the
Standard A may not be taken as the standard for sample test because the amount of the
Standard A in the electrolytic cup is greater than the sample amount of 200ul; the sample
amount of 200ul can be modified in the electrolyte arm unit in the hardware.
5) Air isolation: after the sample cup is filled with the Standard A, click the sampling test
button of "Air Isolation" to ensure that Standard A is completely removed from the electrode.
6) "Empty" is generally set at around 2000. The judgment method is to check whether the
liquid in the electrode unit is completely discharged from the liquid waste pump, in order to
avoid the liquid residue causing crystallization in the pipeline. The screenshot is as shown in
F2.8：

Liquid level L2
Liquid level L1

F2.8

52
After these parameters are set, click the "Save Para" button so that when the machine is powered off or the
software is re-opened, click the "Read Para" button, and the parameters previously set can be read out,
otherwise they will be lost.
The function menu is located in the lower left corner of the debugging tool: see F2.9 below

F2.9
Click "One Point Calibration": the electrolyte instrument will draw the Standard B for One
Point Calibration. The data display area on the right shows the second and fifth columns. The
screenshot is as follows: f3.0

F 3.0
Click "Two Point Calibration": the electrolyte instrument will automatically perform Two
Point Calibration. If the Two Point Calibration is successfully performed, the column of
calibration results will be marked with a tick, and you will hear “beep” and “beep”. The
screenshot is as shown in f3.1 below

53
 F3.1
" Electrode Check": first fill the electrode with the liquid A or B on both ends, click the
"Electrode Check " button, and then the instrument will detect the electrode. The detected
results will be shown in the column of electrode check. The screenshot is as shown below:

The "Voltage Test" and "Electrode Check" buttons are similar. The screenshot is shown as
follows:

Note: after clicking the "Voltage Test" and "Electrode Check" buttons, click the "Quit Check"
button, otherwise the other buttons will not respond and meanwhile the instrument will drain
the liquid in the electrode.
The "Test Urine" and " Test Blood " buttons are used for testing samples. Click " Test Blood
", to manually fill the sample into the sample cup, and press the manual test button on the
panel to test the blood sample. The current test results will be displayed in the column of
analysis results. The screenshot is shown as follows:

54
Multiple blood sample tests can be performed, and the results are displayed in the result area
on the right. The results can be expanded for view, and the screenshot is shown as follows:

"Cleanout" : click this button to clean the pipeline.

" Check Valve " : click this button to check whether each valve is in normal condition, and
there will be a click when the normal valve is opened.
"Test Buzzer" : click this button to check whether the buzzer on the electrolyte motherboard
can make a sound of “beep” and “beep”.
"Version Information" : click this button to obtain the version of the underlying software.
After well setting the parameters, you can click the Cleanout button in the function menu to
check whether the parameters are set correctly and ensure the pipeline is clean. Then, click
the Two Point Calibration button to see whether the value and slope in the data display area
are in the normal range. When the column of calibration results is ticked and the sound of
“beep” and “beep” is heard, the Two Point Calibration is passed. If the calibration fails to
pass, click the Check Valve, Check Pole and Check Voltage buttons to check whether the
valve is working, whether the electrode is in normal condition, and whether the connection
between the electrolyte motherboard and each pump and valve is broken. Secondly, check the
pipeline to see whether their parameters are set reasonably. Moreover, users can also check
whether the electrode and each detection channel are in good contact.

5. Upper Computer Software Operation

Enter the biochemical operation parameter ——> device interface to check whether there is a
tick in the front of the Setup ISE items. In case of no tick, users shall go to hardware to

55
modify iseSetup=1. Set the correct electrolyte communication serial port in the
communication place. Turn off the software and machine and turn on them again, to make
ISE online as usual.
The interface of Setup ISE items can be seen in F3.2.

F3.2
Set the item name, IseConfig. Ini file in the software operation folder: "channelK=1" means
that the K channel is selected, and 0 means that the channel is not selected. The channel name
selected in the IseConfig. Ini file must be the same as in the Setup ISE items.
Set the position and concentration value of the Standard 1 and Standard 2 when the ISE items
are calibrated, which are generally set as Position 1 and Position 2. The correction function is
automatically generated after calibration.

The interface of Setup ISE parameters can be seen in F3.3.

56
 F3.3
In this interface users can also set the parameters of each sampling volume and save them to
the electrolyte lower computer. If the calibration under detection is ticked, one point
calibration will be carried out automatically after completing a ISE sample test.
The default each sample volume added by the diluter is 200UL, and the sample volume can
be set in the hardware:
[ElectrolyteArm]
serumVolume=200
If the serum volume of 200ul is to be modified, then the ISE sampling volume parameters
shall also be modified to ensure that both ends of the electrode are filled with the sample.

57
 Electrode

Add the standard and sample: click the Add button, select ise Calibration, and then click
Confirm, so that calibration tasks of all ise items can be added. You can also click the
"Unselected" button to select the ise items to be calibrated. Moreover, you can also add
biochemical calibration items, or add samples in the Add Sample interface for testing. Before
starting the test, it will be prompted whether two point calibration is required. Click Yes to
proceed to the third step, and then continue the test. Click No to enter the test directly. ISE
calibration results can be searched in the interface of calibration results.

6. Replacement of instrument parts

6.1 Replacement of internal liquid
When the calibration measurement is unstable, the electrolyte or electrode tip can be replaced.
The specific operation is as follows：
Unscrew the electrode tip and empty the electrolyte；
Add the new electrolyte with a syringe；
Install the new electrode tip and tighten and wipe it clean.
6.2. Replacement of pump pipes

After the pump pipe is used for half a year, or if it is confirmed that the pump pipe is

damaged or the pump liquid quantity is insufficient, the pump pipe needs to be replaced.

Please follow the following steps to replace the pump pipe:

1. Loosen the old pump pipes successively.

2. Remove the old pump pipes from the inside out and connect the new pump pipes

successively.

3. Bypass the barrel wheel with the new pump pipes successively.

4. Install the new pump pipes in sequence on the pump pipe support.

58
7. Common Faults and Troubleshooting

Fault Fault
Fault cause Troubleshooting
number phenomenon
a) ISE power is not turned a) Turn on the power；
on； b) Plug in the serial port
b) The serial port line line；
connection is not c) Check the software
ISE not normal. settings and modify
1．
online c) The software settings the correct serial port.
are incorrect d) The sequence of
d) The sequence of internal wires in the
internal wires in the serial port 2-2,3-3; 1-5
serial port is not correct
a) Check the reagent,
observe the liquid at
a) Lack of reagent or the two ends of the
unreasonable number electrode pipeline,
of sampling steps, no and check whether
liquid in the electrode, the pipeline is
or bubble in the pipe blocked. The
b) Operating parameters corresponding
of ISE are not set pipeline can be
Calibration correctly handled according to
2．
fails to pass c) Unstable electrode； the specific prompt.
d) The electrode elastic b) Check the parameters
contact is not in good c) Stabilize the electrode
contact； for 30 minutes
e) Bad electrode d) Reinstall
f) Reagent failure e) Replace the internal
liquid
f) Replace the expiring
reagent

59
 a) Dredge the sample
injector with a syringe
and rinse it；
b) Remove the electrode,
dredge it with a
The sample a) Blocked sample syringe and rinse the
cannot be injector； electrode for three
injected, and b) Blocked electrode； times; remove and
3.
there are c) Pump pipe aging； disassemble the
bubbles in d) Broken or loosened electrode. Clean the
the pipeline; pipeline； single electrode with a
blow ball；
c) Replace the pump
pipe
d) Replace or install the
pipeline；

a) a. Check the margin

of the Standard A and
ISE offline a) Calibration fails to pass Standard B, and carry
4. during b) I/O trigger line out two point
detection connection is abnormal calibration again.
b) b．Reconnect the I/O
trigger line.

60