Pre-Laboratory Sheet

Proteins
1. R – L – Y – E – K – I – I – M – G – F – H – W

Arg-Leu-Tyr-Glu-Lys-Ile-Ile-Met-Gly-Phe-His-Trp

F–H–C–C–T–W–M–E–D–Y–F

Phe-His-Cys-Cys-Thr-Trp-Met-Glu-Asp-Typ-Phe

Write the corresponding 3-‐letter abbreviation of the amino acids in each polypeptide.

2.
Tes A B Test A B
t
Biuret + + Sakaguchi + -
Ninhydrin + + Lead Acetate - +
Xanthoproteic + + FeCl3 + +
Hopkins-‐Cole + +

3. Isoelectric point (pI), also referred to as isoelectric pH, is the pH at which a specific molecule has no net
migration taking place in an electric field. The overall charge of the molecule in the isoelectric point is
zero or a neutral charge. This principle is used in the preparation of cottage cheese (kesong puti).
During acid coagulation of milk, as it reaches a pH of 4.6, casein, the main protein in milk, would then
separate itself from the rest of the milk components (like water and milk fat) and would 'clump
together' with other casein protein, thus forming cottage cheese. That is because the pH level of 4.6 is
isoelectric point of casein protein.

4. In the hospital setting, denaturation through exposure to heat is often used in sterilizing medical
instruments and supplies from bacteria and viruses. The sterilization of medical equipment uses
the procedure known as moist heat sterilization to denature the protein of the bacteria and
viruses, thus destroying it. Another example of denaturation used in the hospital setting is for the
proper disposal of expired controlled drugs (CDs). This procedure is required to avoid expired
drugs to be ‘reuse or retrievable’, thus preventing the misuse or selling of expired controlled drugs.
Registered pharmacists from hospitals and medical practices are usually the one that does the
denatures and disposal procedure of expired CDs.
5.
Test Name of the Amino acid Positive visible result
group identified
Ninhydrin all containing α- All (except for color changes to a blue-violet
NH2 group proline) amino solution
(except for acids with nonpolar,
proline and polar, acidic, and
hydroxyproline) basic side chain
Xanthoproteic amino acid with Phenylalanine, yellow-orange precipitate in
benzene ring in Tyrosine, the solution
their side chain Tryptophan
(aromatic group)
Hopkins-‐Cole presence in Tryptophan presence of a violet ring
indole group
Sakaguchi amino acid with Arginine color changes to a red solution
guanido group
Lead Acetate presence in thiol Cysteine black colored precipitates in
group the soultion
FeCl3 amino acid with Tyrosine color changes to a violet
phenolic group solution