Iron, Ferrous, 8146

Iron, Ferrous DOC316.53.01049

1-10 Phenanthroline Method1 Method 8146

0.02 to 3.00 mg/L Powder Pillows or AccuVac® Ampuls
Scope and Application: For water, wastewater and seawater
1 Adapted from Standard Methods for the Examination of Water and Wastewater, 15th ed. 201 (1980)

Test preparation

How to use instrument-specific information

The Instrument-specific information table displays requirements that may vary between
instruments. To use this table, select an instrument then read across to find the corresponding
information required to perform this test.

Table 195 Instrument-specific information

Powder pillows AccuVac Ampuls

Instrument
Sample cell Cell orientation Sample cell Adapter

DR 6000 2495402 Fill line faces right 2427606 —

DR 5000 2495402 Fill line faces user 2427606 —

DR 3900 2495402 Fill line faces user 2427606 LZV846 (A)

DR 3800, DR 2800, DR 2700 2495402 Fill line faces right 2122800 LZV584 (C)

Before starting the test:

For more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using
deionized water instead of the sample. Subtract the reagent blank value from the final results or perform a reagent blank
adjust.

Analyze samples as soon as possible to prevent air oxidation of ferrous iron to ferric iron, which is not determined.

If ferrous iron is present, an orange color will form after adding the reagent.

Collect the following items:

Description Quantity

Powder Pillow Test:

Ferrous Iron Reagent Powder Pillows 1

Sample Cells (see Instrument-specific information) 2

AccuVac Test:

Ferrous Iron Reagent AccuVac® Ampuls 1

Beaker, 50 mL (AccuVac test) 1

Sample Cell (see Instrument-specific information) 1

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Collect the following items:

Description Quantity

See Consumables and replacement items for reorder information.

1-10 Phenanthroline method for powder pillows

Stored Programs

255 Iron, Ferrous

Start

1. Select the test.
Insert an adapter if
required (see Instrument-
specific information).
Refer to the user manual
for orientation.
2. Fill a clean graduated
mixing cylinder with 25 mL
of sample.
3. Prepared Sample: 4. Insert a stopper and
Add the contents of one invert to mix. Undissolved
Ferrous Iron Reagent powder does not affect
powder pillow to the accuracy.
cylinder.

5. Start the instrument 6. Blank Preparation: 7. Fill a second sample 8. When the timer
timer. Fill a sample cell with cell with the prepared expires, insert the blank
A three-minute reaction 10 mL of sample. sample from the mixing into the cell holder.
period will begin. cylinder in step 4.

Zero Read

9. ZERO the instrument. 10. Insert the prepared 11. READ the results in
The display will show: sample into the cell holder. mg/L Fe2+.
0.00 mg/L Fe2+

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1-10 Phenanthroline method for AccuVac® Ampuls

Stored Programs

257 Iron, Ferrous AV

Start

1. Select the test.
Insert an adapter if
required (see Instrument-
specific information).
Refer to the user manual
for orientation.
2. Blank Preparation:
Fill a round sample cell
with 10 mL of sample.
3. Prepared Sample: 4. Quickly invert the
Fill a Ferrous Iron Reagent Ampul several times
AccuVac® Ampul with to mix.
sample from the beaker.
Keep the tip immersed
while the Ampul fills
completely.

5. Start the instrument 6. Wipe the blank and 7. Wipe the Ampul and
timer. insert it into the cell holder. insert it into the cell holder.
A three-minute reaction ZERO the instrument. READ the results in
period will begin. The display will show: mg/L Fe2+.

0.00 mg/L Fe2+

Sample collection, preservation and storage

• Collect samples in plastic or glass bottles.
• Analyze samples as soon as possible after collection.

Accuracy check
Standard solution method
Note: Refer to the instrument user manual for specific software navigation instructions.

Required for accuracy check:

• Ferrous Ammonium Sulfate, hexahydrate, 0.7022 g
• 1 L Class A volumetric flask
• 100 mL Class A volumetric flask
• Deionized water

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• Analytical balance
• 2 mL Class A volumetric pipet and pipet filler

1. Prepare a 100 mg/L Fe2+ ferrous iron stock solution as follows:

a. Dissolve 0.7022 grams of Ferrous Ammonium Sulfate, hexahydrate, in deionized water.

b. Dilute to one liter in a Class A volumetric flask.
c. In a 100 mL Class A volumetric flask, dilute 2.00 mL of this solution to 100 mL with
deionized water to make a 2.0 mg/L standard solution. Prepare this solution immediately
before use.

2. Follow the 1-10 Phenanthroline method for powder pillows or the 1-10 Phenanthroline method
for AccuVac® Ampuls test procedure.

3. To adjust the calibration curve using the reading obtained with the 2.00-mg/L Standard
Solution, select Options>More>Standard Adjust from the instrument menu.

4. Turn on the Standard Adjust feature and accept the displayed concentration. If an alternate
concentration is used, enter the concentration and adjust the curve to that value.

Method performance

Precision Sensitivity
Program Instrument Standard 95% Confidence Limits of Concentration change
Distribution per 0.010 Abs change
255 DR 5000 2.00 mg/L Fe2+ 1.99–2.01 mg/L Fe2+ 0.021 mg/L Fe2+
257 DR 2800 2.00 mg/L Fe2+ 1.98–2.02 mg/L Fe2+ 0.023 mg/L Fe2+

Summary of method
The 1-10 phenanthroline indicator in the Ferrous Iron Reagent reacts with ferrous iron (Fe2+) in the
sample to form an orange color in proportion to the iron concentration. Ferric iron (Fe3+)does not
react. The ferric iron concentration can be determined by subtracting the ferrous iron concentration
from the results of a total iron test. Test results are measured at 510 nm.

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Consumables and replacement items

Required reagents
Description Quantity/Test Unit Catalog number
Ferrous Iron Reagent Powder Pillows 1 100/pkg 103769
OR
Ferrous Iron Reagent AccuVac® Ampuls 1 25/pkg 2514025

Required apparatus
Description Quantity/Test Unit Catalog number
Beaker, 50 mL 1 each 50041H
Sample cell, 10 mL round, 25 x 54 mm 1 each 2122800
Sample cell, 10 mL round, 25 x 60 mm 1 6/pkg 2427606
Sample cell, 10 mL square, matched pair 2 2/pkg 2495402

Recommended standards and apparatus

Description Unit Catalog number
Balance, analytical, 80 g x 0.1 mg 100–240 VAC each 2936701
Ferrous Ammonium Sulfate, hexahydrate, ACS 113 g 1125614
Flask, volumetric, 1000 mL each 1457453
Pipet filler, safety bulb each 1465100
Pipet, volumetric, 2.00 mL each 1451535
Water, deionized 4L 27256
Wipers, disposable 280/pkg 2097000

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