Experiment No.

2
Fats and Lipids

Objective:
 To identify the physical properties of fats and lipids

Materials:
Isopropyl alcohol, acetone, paint thinner, cooking oil, baby oil, glycerol (glycerine), muriatic acid (HCl),
small glasses, syringe/medicine dropper, oslo paper

Procedure:

A. Spotting Effect

1. On ¼ oslo paper, place on separate spots/corner a drop of the following: alcohol, cooking oil, baby oil,
paint thinner, acetone. Label each sample. Let the paper stand for 5 minutes and note which corner
leaves a translucent spot. Compare the results.

Observation
Alcohol

Cooking oil

Baby oil

Paint thinner

Acetone

B. Solubility

1. Prepare 4 small glasses and place in each 10 ml of water, paint thinner, muriatic acid, isopropyl
alcohol, and acetone. Add 3 drops of cooking oil in each small glasses. Stir. Record the results.

2. Repeat the procedure using glycerol.

Solvent Cooking oil Glycerol

Water

Paint thinner

Muriatic acid
 Isopropyl alcohol

Acetone

Questions:

1. What is the difference between a fat and oil, physically and chemically?

2. What are glycerides? Why is an acrolein test a general test for fats and oils?

3. Differentiate saturated fats from unsaturated fats?

4. Give the structure and properties of glycerol.

Conclusion: