CRYOPRECIPITATE PREPARATION

WRITTEN BY: Fayzah Alshammari

DATE: 04/02/2021

Cryoprecipitate AHF:
Cryoprecipitated Antihemophiliac Factor (CRYO) is created by thawing a frozen unit of Fresh
Frozen Plasma (FFP) at 1-6°C until a slushy precipitate forms. This precipitate is a rich source
of factors VIII and XII, vWF, and fibrinogen.

Preparing Cryoprecipitate:

 Determine whether FFP must be ≥200 g to prepare Raw Cryo.

 FFP thaw at 1 to 6 C by placing the bag in a 1 to 6 C circulating waterbath or in a
refrigerator. If thawed in a waterbath, use a plastic overwrap keep container
ports dry.
 Centrifuge the plasma at 1 to 6 C using a “heavy spin” to separate the plasma
from the cryoprecipitate.
 Use plasma expressor to allow the supernatant to flow slowly into a transfer bag.
 Leaving the cryoprecipitate adhering to the sides of the primary bag to prevent
the cryoprecipitate from dissolving
 10 to 15 mL of supernatant plasma may be left in the bag for resuspension of the
cryoprecipitate after thawing.
 Cryoprecipitate should be refrozen within 1 hour of preparation and store at -18
or cooler.
 Cryo expires 12 months from date the phlebotomy, not from the date the cryo
was prepared.
 Cryoprecipitated AHF should be rapidly thawed at 30 to 37 C and transfused
within 6 hours of thawing or within 4 hours of pooling
 Once cryoprecipitate is thawed, the FDA recommends storing at room
temperature until transfused.

Note:

For Pooling Cryoprecipitate all products should be the same ABO (Rh matching is not
necessary).
QC & Parameters (AABB):

make sure that 75% of tested unit:

 Factor VIII levels ≥ 80 IU

 Fibrinogen levels ≥ 150 mg

Indication of Cryoprecipitated AHF:

1. Treatment of factor VIII deficiency (Hemophilia A).

2. Von Willebrand’s.
3. Hypofibrinogenemia.
4. Treatment of uremic coagulopathy.

Dosage of cryoprecipitate AHF:

 The typical adult dose is 5-10 units pooled.

 Pediatric dose for CRYO is one to two single units/10 kg body weight.