BIOTECHNOLOGY

BIOENGINEERING
Volume 114, Number 12, December, 2017

REVIEWS

Bioprocess Engineering and Supporting Technologies

2696 Biosimilars: Key Regulatory Considerations and Similarity Assessment

Tools
C.F. Kirchhoff, X.-Z.M. Wang, H.D. Conlon, S. Anderson, A.M. Ryan,
and A. Bose

page 2807

Engineering Science of Biological Systems

2706 Development of Aptamers Against Unpurified Proteins

S. Goto, K. Tsukakoshi, and K. Ikebukuro

Systems Biotechnology

2717 Quantitative Intracellular Flux Modeling and Applications in

Biotherapeutic Development and Production Using CHO Cell Cultures
Z. Huang, D.-Y. Lee, and S. Yoon

page 2762

ARTICLES

Biocatalysis, Protein Engineering and Nanobiotechnology

2729 Comparative Analysis of Plant-Produced, Recombinant Dimeric IgA

Against Cell Wall ␤-Glucan of Pathogenic Fungi
C. Capodicasa, M. Catellani, I. Moscetti, C. Bromuro, P. Chiani,
A. Torosantucci, and E. Benvenuto

2739 Recombinant RNA Polymerase From Geobacillus sp. GHH01 as Tool for
Rapid Generation of Metagenomic RNAs Using In Vitro Technologies
B.M. Kinfu, M. Jahnke, M. Janus, V. Besirlioglu, M. Roggenbuck, R. Meurer, B&B Video Highlights
L. Vojcic, M. Borchert, U. Schwaneberg, J. Chow, and W.R. Streit

2691
 Biofuels and Environmental Biotechnology

2753 Mechanisms of Nitrous Oxide (N2O) Formation and Reduction in

Denitrifying Biofilms
F. Sabba, C. Picioreanu, and R. Nerenberg

page 2931 2762 Kinetics Based Reaction Optimization of Enzyme Catalyzed Reduction
of Formaldehyde to Methanol With Synchronous Cofactor Regeneration
F. Marpani, Z. Sárossy, M. Pinelo, and A.S. Meyer

Bioprocess Engineering and Supporting Technologies

2771 Cascading Effect in Bioprocessing—The Impact of Mild Hypothermia on

CHO Cell Behavior and Host Cell Protein Composition
C.H. Goey, J.M.H. Tsang, D. Bell, and C. Kontoravdi

Cellular and Metabolic Engineering

2782 Metabolic Engineering of Isopropyl Alcohol-Producing Escherichia coli

Strains With 13C-Metabolic Flux Analysis
N. Okahashi, F. Matsuda, K. Yoshikawa, T. Shirai, Y. Matsumoto, M. Wada,
and H. Shimizu

2794 Combination of Traditional Mutation and Metabolic Engineering to

Enhance Ansamitocin P-3 Production in Actinosynnema pretiosum
Z.-Q. Du, Y. Zhang, Z.-G. Qian, H. Xiao, and J.-J. Zhong

2807 Recombinant Lactococcus lactis for Efficient Conversion of Cellodextrins

into L-Lactic Acid
C. Gandini, L. Tarraran, D. Kalemasi, E. Pessione, and R. Mazzoli

2818 Dynamic Monitoring of p53 Translocation to Mitochondria for

the Analysis of Specific Inhibitors Using Luciferase-Fragment
Complementation
N. Noda, R. Awais, R. Sutton, M. Awais, and T. Ozawa

2828 Escherichia coli “TatExpress” Strains Super-Secrete Human Growth

Hormone into the Bacterial Periplasm by the Tat Pathway
D.F. Browning, K.L. Richards, A.R. Peswani, J. Roobol, S.J.W. Busby, and
2931
C. Robinson

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2837 Formic Acid as a Secondary Substrate for Succinic Acid Production by
Metabolically Engineered Mannheimia succiniciproducens
J.H. Ahn, J. Bang, W.J. Kim, and S.Y. Lee

2848 Combinatorial Genome and Protein Engineering Yields Monoclonal

Antibodies With Hypergalactosylation From CHO cells
C.-y. Chung, Q. Wang, S. Yang, S.A. Ponce, B.J. Kirsch, H. Zhang, and
M.J. Betenbaugh

Engineering Science of Biological Systems page 2883

2857 NMR Investigation of Water Diffusion in Different Biofilm Structures

M.P. Herrling, J. Weisbrodt, C.M. Kirkland, N.H. Williamson, S. Lackner,
S.L. Codd, J.D. Seymour, G. Guthausen, and H. Horn

2868 Bidirectional Reporter Assay Using HAL Promoter and TOPFLASH

Improves Specificity in High-Throughput Screening of Wnt Inhibitors
K. Yamaguchi, C. Zhu, T. Ohsugi, Y. Yamaguchi, T. Ikenoue, and
Y. Furukawa

Synthetic Biology

2883 Controlling Localization of Escherichia coli Populations Using a

Two-Part Synthetic Motility Circuit: An Accelerator and Brake
R. McKay, P. Hauk, H.-C. Wu, A.E. Pottash, W. Shang, J. Terrell,
G.F. Payne, and W.E. Bentley

2896 CRISPRi Repression of Nonhomologous End-Joining for Enhanced

Genome Engineering via Homologous Recombination in
Yarrowia lipolytica
C. Schwartz, K. Frogue, A. Ramesh, J. Misa, and I. Wheeldon

Systems Biotechnology

2907 A Dynamic Metabolic Flux Analysis of ABE (Acetone-Butanolethanol)

Fermentation by Clostridium acetobutylicum ATCC 824, With
Riboflavin as a by-Product
X. Zhao, M. Kasbi, J. Chen, S. Peres, and M. Jolicoeur

page 2857

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 Tissue Engineering and Delivery Systems

2920 Alginate-Gelatin Encapsulation of Human Endothelial Cells Promoted

Angiogenesis in In Vivo and In Vitro Milieu
S. Nemati, A. Rezabakhsh, A.B. Khoshfetrat, A. Nourazarian, Ç.B. Avci,
B.G. Bagca, H.A. Sardroud, M. Khaksar, M. Ahmadi, A. Delkhosh,
E. Sokullu, and R. Rahbarghazi

2931 Codelivery of Anti-Cancer Agents via Double-Walled Polymeric

Microparticles/Injectable Hydrogel: A Promising Approach for
Treatment of Triple Negative Breast Cancer
P. Davoodi, W.C Ng, M.P. Srinivasan, and C.-H. Wang

COMMUNICATION TO THE EDITOR

Systems Biotechnology

2947 Impact of Growth Mode, Phase, and Rate on the Metabolic State of the
Extremely Thermophilic Archaeon Pyrococcus furiosus
P.A. Khatibi, C.-j. Chou, A.J. Loder, J.V. Zurawski, M.W.W. Adams, and
R.M. Kelly

page 2848 2955 Erratum

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Cover Legend
The cover image, by Zhuangrong Huang et al., is based on the Review Quantitative
intracellular flux modeling and applications in biotherapeutic development and production using
CHO cell cultures, DOI: 10.1002/bit.26384.
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