Name ______________________ Date_____ / _____ / _________

DIHYBRID (TWO TRAITS) CROSSES

When two traits are considered the number of possible genotype in parents and offspring
increases. A cross involving two phenotypes I called a dihybrid cross. Suppose that black hair
(H) is dominant over blonde hair (h) and brown eyes (E) are dominant over blue eyes (e).
Consider the following crosses between two parents:
Consider the following crosses between two parents:

1. The father is heterozygous ate both loci and has black hair and brown eyes while the
mother has blonde hair and blue eyes.
Genotype of father-HhEe
Genotype of mother-hhee
In the Punnett square below, complete the lists of gametes from the parents. Then, fill
in the boxes.

HE He hE he
he HhEe Hhee hhEe hhee
he HhEe Hhee hhEe hhee
25 %
he HhEe Hhee hhEe hhee
he HhEe Hhee hhEe hhee

2. Both parents are heterozygous at both loci.

Genotype of father - ________
Genotype of Mother-________
Fill in the Punnett square below.

BE be bE be
BE HHEE HHEe HhEE HhEe
Be HHEe HHee HhEe Hhee
6.25 %
bE HhEE HhEe hhEE hhEe
be HhEe Hhee hhEe hhee

For each of the crosses above, indicate the expected frequencies of individuals with blonde
hair and blue eyes on the blanks shown.

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