BMS533 PRAC#5 : SOP for Biosafety Cabinet

Objective

To draft SOP for the use of Type II Biosafety Cabinets

Outcomes

I. Know - the various types of Biosafety Cabinets

II. Know - how to use a Biosafety Cabinet correctly
III. Do - prepare a set of guidelines for using Biosafety Cabinets
IV. Feel - confident in using Biosafety Cabinets to carry out work of hazadous nature

SOPs
An SOP is a set of instructions or steps someone follows to complete a job safely, with no
adverse impact on animals or the environment, and maximizes operational and production
requirements.

Tips for writing an SOP

In this exercise you will write a simple SOP. Follow these tips and principles :
Tip 1 : Begin each step with a single action verb e.g. "Open"
Tip 2 : Keep each step simple
Tip 3 : Identify the person who will perform the step
Tip 4 : Use quantitative information e.g. 5 ml
Tip 5 : List multiple objects
Tip 6 : Emphasize important information e.g. bold, italics or underline
Tip 7 : Most importantly, think like the USER
Tip8 : Use Behavorial Observation data (BODs) to monitor compliance

Exercise

1. You will taken on a demonstration tour of the lab to identify the various types of
Biosafety Cabinets
2. After the demo, discuss and write an SOP for the use of Type II Biosafety Cabinets.

Use the template below. Submit the guidelines as your report.

SOP for Use of Type II Biological Safety Cabinets

Facility : ..............

Person-in-charge : .....

Purpose : ........

Scope : .......

Materials : .......

Last Revision : ..........

This SOP is intended for personnel working with biological materials

1.0 Procedures

A. Preparation
1. Wear appropriate personal protective equipment like:
 glove [ BOD : Is the user wearing gloves? ]
 ......

2. ..............

B. Before you switch on the BSC

11. Check that the BSC is certified for use, and certification is not expired.
12. Check that the.......................

C. Turn on the BSC.....

D. Turn off the BSC.....
2.0 Biosafety

A. Personal Protective Equipment

General PPE e.g. lab coat, gloves, goggles and face mask if necessary.
Use heavy duty thick autoclave gloves when unloading hot material.

B. Clean up
 If there is water soillage on the floor, mop dry
 If there is material spillage in the autoclave, record in the logbook, inform the lab director
and carry out SOP # 1036
 If the control panel is wet, wipe dry with clean paper towel
 Periodically check and adjust the water level in the steam outlet container

If spill is large or unmanageable call Environmental Safety @ X-XXXX

3.0 Responsibility

3.1 Supervising personnel - to ensure that the laboratory personnel performing these tasks
are trained appropriately and that proper procedures are followed.
3.2 Laboratory personnel - to accurately record all required data and results.
3.3 Laboratory personnel - to document any deviation from these procedures, and consult
supervising personnel on the matter..

4.0 Reference

1. http://www.csbsju.edu/biology/chemical-inventory-and-safety/biosafety-plan/biosafety-
emergency-procedures
2. ......
3. ......
4. ......