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PDF Makalah Mata Kuliah Manajemen Laboratorium Laboratorium Kultur Jaringan DL
PDF Makalah Mata Kuliah Manajemen Laboratorium Laboratorium Kultur Jaringan DL
@CBCFCH
FCMN]C[N]OZ@
@C[C BZFOCH @CACGJ@JA FCMN]C[N]OZ@
(FCMN]C[N]OZ@ BZF[Z] GC]OAECA [Z@MZHCA)
[ Z@MZHCA)
KO\Z\ZA NFJH7
AO@ 7 H9<3?:9:
BJFC\ 7 YJAK.
YJAK. MONFNEO
MONFNEO L
BC[C YJAECA[C]
Yugo syubur scyc pcagctbca bjhckorct Cffch _cae @chc Jsc bcrjac kjaeca
rch`ct, bcruaoc sjrtc tcudob kca hokcych-ayc scyc kcpct `jayjfjscobca `cbcfch ycae
mjrgukuf ]calcaeca Kjach Fcmnrctnrou` kca @cacgj`jaayc oao kjaeca mcob.
[okcb fupc guec scyc `jaeulcpbca
`jaeulcpbca tjro`cbcsoh pckc
pckc Mcpcb Crocako, \.Yk., @.\o
sjfcbu knsja `ctc bufoch @cacgj`ja Fcmnrctnrou`, ycae tjfch `j`mjrbca tuecs oao
bjpckc scyc kca `j`mo`moae scyc kcfc` `jaejrgcbca `cbcfch oao. \cyc scaect
mjrhcrcp `cbcfch oao kcpct mjreuac mceo pj`mclc uatub `jac`mch pjaejtchuca.
\cyc guec `jayckcro sjpjauhayc mchwc kokcfc` `cbcfch oao tjrkcpct bjburcaeca kca
gcuh kcro bctc sj`purach. Nfjh sjmcm otu, scyc
sc yc mjrhcrcp ckcayc brotob kca usufca kj`o
pjrmcobca `cbcfch ycae tjfch scyc muct oao. \j`nec `cbcfch ycae sjkjrhcac oao kcpct
kopchc`o nfjh pcrc pj`mclc. Kca kcpct mjreuac mceo scyc sjakoro kca guec pcrc
pj`mclc. \jmjfu`ayc scyc `nhna `ccd cpcmofc tjrkcpct bctc-bctc ycae burcae
mjrbjacae.
Yjaufos
o
KCD[C] O\O
oo
MCM O
YJAKCHZFZCA
3
L. [uguca
3. Zatub `jaejtchuo ec`mcr ctcu strubtur kjach kca tctc fjtcb fcmnrctnrou`
buftur gcroaeca tu`muhca
=. Zatub `jaejtchuo pjrcfctca fcmnrctnrou` buftur gcroaeca tu`muhca
<. Zatub `jaejtchuo kcdtcr mchca fcmnrctnrou` buftur gcroaeca tu`muhca
;. Zatub `jaejtchuo tctc bjfnfc fcmnrctnrou`
:. Zatub `jaejtchuo cturca tctc tjrtom fcmnrctnrou`
4. Zatub `jaejtchuo stcakcr `utu fcmnrctnrou`
K. @cadcct
@cadcct pj`muctca `cbcfch oao ckcfch uatub kcpct koeuacbca sjmceco mchca
pjaecgcrca ko mokcae pjakokobca.
=
MCM OO
[OAGCZCA YZ\[CBC
<
;
:
]ucaeca oao mocscayc koeuacbca uatub tj`pct `jaecacfosos, `jaec`cto
4
`j`mjrsohbca cfct-cfct (cfct-cfct ejfcs sjpjrto pjtro, mntnf, kff), pjrsocpca kca
stjrofoscso `jkoc, kca pjayo`pcaca cfct-cfct ejfcs. \jmuch wjstcdjf uatub
`jalulo ycae
ycae kofjaebcpo kjaeca brca uatub
uatub cforca cor `jaecfor guec
guec
kopjrfubca uatub `j`mjrsohbca cfct-cfct mjrmchca ejfcs. \jfcoa otu
kopjrfubca `jgc ycae pjr`ubccayc kofcposo kjaeca mchca ycae `ukch
komjrsohbca. Korucaeca oao tjrkoro kcro7
- Nvja
- Cutnbfcd
- [j`pct lulo
- Bn`pnr ecs
5
]ucae trcasdjr/ pjacac`ca `jrupcbca rucae ycae
ycae koeuacbca
koeuacbca uatub
osnfcso, oanbufcso kca sumbuftur (pjagcrcaeca) pckc bnakoso stjrof. ]ucaeca oao
sjkcpct `uaeboa mjmcs kcro kjmu kca hjwca bjlof, pjaeeuacca CL scaect
kocagurbca kcfc` rucaeca oao. ]ucae trcasdjr kofjaebcpo kjaeca pjrcfctca
sjmceco mjrobut7
- Fc`oacr cor dfnw lcmoajt, mosc guec jabcs
- Cfct-cfct kosjbso poscu mjkch/slcpjf,poasjt,spctufc kca euatoae.
- Hcak sprcyjr ycae mjroso cflnhnf 59%
- Fc`pu Muasja
8
?
39
33
`cupua uatub euru. ]ucae pjayo`pcaca ctcu eukcae tjrutc`c koeuacbca uatub
`jayo`pca mchca-mchca pjrsjkocca (tjr`csub mchca bo`oc) kca cfct-cfct ycae
pjaeeuaccaayc tokcb sjtocp scct (gcrcae).
\jfcoa rucaeca-rucaeca tjrsjmut, `uaeboa guec sjmuch fcmnrctnrou`
`j`ofobo rucae ejfcp (kcrb rnn`), rucaeca spjso`ja, rucaeca bhusus uatub
pjayo`pcaca mchca-mchca bo`oc kca rucae ck`oaotrcso / stcd . Hcf oao kokcscrbca
ctcs pjrto`mcaeca bjc`caca mjrmceco pjrcfctca fcmnrctnrou` kca bjayc`caca
pcrc pjaeeuac fcmnrctnrou`. Yjayo`pcaca cfct-cfct ko kcfc` eukcae tokcb mnfjh
kosctubca kjaeca mchca bo`oc. Kj`oboca pufc pjayo`pcaca cfct-cfct ejfcs tokcb
3=
Zburca rucae utc`c fjmoh mjscr kcro pckc uburca rucae pjrsocpca kca rucae
pjayo`pcaca. Lnatnh cpcmofc fucs fcatco uatub sjmuch mcaeuaca fcmnrctnrou`
399`=, ;=
;= 59 “ 89 `= koeucacbca uatub
uatub rucae utc`c tj`pct prcbtobu`.
prcbtobu`. ]ucae
3<
3;
jbspfcaa csjpjrtomjruburca
`olrna buftur
buftur
prntnp
ntnpffc , buftur
buftur pnffja
kca fcoa-fcoa.
4 Fj`cro js Zatub jayo`pca stnb-
stnb ` koc buftur cecr
tokcb llj
jpct ruscb
3:
34
35
38
3?
fucr
`jaebnatc`oacso tokcb
bnakoso
mntnf.
=3 Yopjt Yfcstob
Yopjt Yfcstob Yopjt koeuacbca uatub
`j`oakchbca sjgu`fch
lcorca kcro wckch sctu bj
wckch ycae fcoa.
== Yjaeckub bclc
Yjaeckub Yjaeckub bclc koeuacbca
Bclc uatub `jaeckub fcrutca-
fcrutca ycae ckc pckc buftur
gcroaeca.
=9
muasja.
gcru` nsjZatub stjrofoscso
ctcu ycae fcoa,
mceoca cpo ycae pcfoae
lnlnb uatub
`j`ogcrbcaayc ckcfch
mceoca cpo ycae mjrwcrac
moru (pcfoae pcacs).
=: Ejfcs Zbur/Lyfoakjr
Ejfcs ubur koeuacbca uatub `jaeubur
vnfu`j fcrutca ycae
mjatubayc sjpjrto lnrnae
ctcupua ejfcs ycae
`j`puayco uburca vnfu`j
`ofofotjr mjrdcrocso.
=4 \jmceco tj`pct
Bjrcagcae pjayo`pcaca mntnf buftur
mntnf buftur ycae tjfch kostrjrofoscso.
=5 \jmceco tj`pct
Fj`cro fcrutca pjayo`pcaca mjrmceco
bo`oc `clc` fcrutca bo`oc.
=3
jbspfca, kocatcrcayc ckcfch kjtjreja, cfbnhnf, lfnrnx, cquckjst stjrof, kca sporotus
ycae kcpct koeuacbca uatub stjrofoscso pjr`ubcca FCD ctcu uatub lcorca kcfc`
muasja.
@jkoc @\ `jrupcbca `jkoc buftur gcroaeca ycae mcaycb koeuacbca uatub
`jaebufturbca mjrmceco gjaos tcac`ca, bcrjac `jkoc oao `jaecakuae uasur hcrc
`cbrn kca `obrn ycae fjmoh fjaebcp komcakoaebca pjaj`u-pjaj`u sjmjfu`ayc.
\jtjfch pjaj`uca `jkoc @\, mcaycb mjrbj`mcae `nkodobcso-`nkodobcso `jkoc
uatub tuguca tjrtjatu, lnatnh `jkoc Aotslh & Aotslh (3?4?) uatub buftur cathjr
kca `jkoc \H (\lhjab & Hokjmrcat) uatub buftur bcfus `nanbntof kca kobntof
@jkoc TU (Tcloa & Ujat) kca `jkoc nrecaob ycae koeuacbca uatub
pjrmcaycbca caeerjb, sjrtc `jkoc UY@ (Unnky Yfcat @jkoc) uatub tcac`ca
mjrbcyu, ctcu tcac`ca pjrku ctcu pnhna
pnhna mjrbcyu.
==
=<
3. Yjrjalcacca
Yjrjalcacca oao ko`cbsukbca uatub `jrjalcacbca bnasjp kcro suctu
fcmnrctnrou` otu sjakoro.Bcrjac sjmuch fcmnrctnrou` komcaeua uatub tuguca
tjrtjatu. Crtoayc sjmjfu` fcmnrctnou` otu komcaeua hcrus tchu kufu uatub
bjpjrfuca cpc kca uatub kopcbco socpc fcmnrctnrou` tjrsjmut.
Yjrjalcacca `jrupcbca sjmuch prnsjs pj`oborca ycae sostj`ctos, cacfotos,
fneos tjatcae bjeoctca ycae hcrus kofcbubca, fcaebch-fcaebch, `jtnkj, \K@,
tjacec kca kcac ycae komutuhbca uatub `jalcpco tuguca ycae tjfch kotjatubca
sjlcrc jdjbtod kca jdosoja.Yjrjalcacca oao ko`cbsukbca uatub `jrjalcbca
bnasjp kcro suctu fcmnrctnrou` otu sjakoro. sjmjfu` fcmnrctnou` otu komcaeua
hcrus tchu kufu uatub bjpjrfuca cpc kca uatub kopcbco socpc fcmnrctnrou`
tjrsjmut. @oscfayc fcmnrctnrou` ycae cbca koeuacbca uatub pj`mjfcgcrca
Monfneo ko \jbnfch @jajaech tjatuayc cbca `j`ofobo mjatub ycae mjrmjkc
kjaeca fcmnrctnrou` uatub pjajfotoca. Kj`oboca pufc, fcmnrctnrou` uatub
pjajfotoca ctcu pjrlnmcca dosonfneo tu`muhca cbca mjrmjkc kjaeca
kjaeca fcmnrctnrou`
uatub jbnfneo.Yckc u`u`ayc mjatub, uburca kca tctc rucae suctu fcmnrctnrou`
kokjscoa sjkj`oboca rupc sjhoaeec pj`cbco fcmnrctnrou` `ukch `jfcbubca
cbtovotcsayc
=. Yjactcca
[ctc fjtcb pjrcfctca ckcfch suctu mjatub uschc pjaecturca pjaj`pctca
pjrcfctca ko fcmnrctnrou`, sjhoaeec fcmnrctnrou` tjrsjmut mjrwuguk kca
`j`jauho pjrsycrctca uatub mjrnpjrcso.
[ctc fjtcb pjrcfctca ckcfch suctu mjatub uschc pjaecturca pjaj`pctca
pjrcfctca ko fcmnrctnrou`, sjhoaeec fcmnrctnrou` tjrsjmut mjrwuguk kca
`j`jauho pjrsycrctca uatub mjrnpjrcso. Bctc pjaecturca kcfc` bcfo`ct ko ctcs
`jaecakuae `cbac ycae scaect fucs, ycotu mchwc kcfc` `jwugukbca suctu
fcmnrctnrou` ycae fcycb npjrcso kopjrfubca pjaj`pctca pjrfctca ycae tjrsusua
=;
kcfc` fcmnrctnrou` ycae kohcrcpbca, mjeotu pufc kjaeca kcjrch bjrgc hcrus
`j`ofobo fucs ycae `j`uaeboabca pjaeeuac, pjbjrgc, npjrctnr kcpct mjrejrcb
mjmcs, c`ca kca ayc`ca, ko sc`poae fcfu foatcs mchca ycae cbca koeuacbca
=:
fcmnrctnrou`.
m) @csub bj kcfc` rucae fcmnrctnrou` hcrus mjrpcbcoca rcpo kca snpca.
l) [okcb kopjrbjacabca `cbca, `oau`, kca `jrnbnb ko kcfc` rucae
fcmnrctnrou`.
k) Kofcrcae `jruscb kca `jaentnro rucae kca pjrcfctca fcmnrctnrou`.
j) Kofcrcae mjrmuct eckuh kcfc` rucae fcmnrctnrou`.
d) [okcb kopjrbjacabca `jaec`mof kca `j`oakchbca cfct/mchca kcfc`
rucaeca fcmnrctnrou` tcapc ozoa pjtuecs fcmnrctnrou`.
e) [okcb kopjrbjacabca `j`mjrobca/`j`oagc`bca cfct/mchca `ofob
=4
h) Cpcmofc tjrgcko bjruscbca cfct, mcob bcrjac bjscfchca tctc bjrgc ctcu bcrjac
sjmcm-sjmcm fcoa, pjaeeuac dcsofotcs hcrus sjejrc `jfcpnrbca bjpckc
Bjpcfc Fcmnrctnrou` ctcu ycae mjrtcaeeuaegcwcm. Mocyc
Yjaeecatoca/pjrmcobca bcrjac bjscfchca pj`cbcoca sjpjauhayc
komjmcabca bjpckc pjaeeuac.
o) \jtocp bcfo sjfjsco `jaeeuacbca cfct, pjaeeuac kohcrusbca `jajfoto
bjfjaebcpca cfct kca clljssnrojs cfct tjrbcot, sjrtc `j`mjrsohbca kca
`jaej`mcfobcaayc bj tj`pct sj`ufc.
g) Yjaeeuac dcsofotcs tokcb kopjrbjacabca `j`mcwc `cbcaca, `oau`ca kca
=5
`) Yjaeeuac dcsofotcs tokcb kopjrbjacabca `jayjrtcbca nrcae fcoa ycae tokcb
ko`oatcbca ogoa uatub obut mjbjrgc ctcu
ct cu `jauaeeu ko rucae fcmnrctnaou`.
a) Yjaeeuac dcsofotcs kcpct `jaeeuacbca mchca bo`oc ko fcmnrctnrou` ctcs
ycae mjrscaebutca.
l) @chcsoswc cbca komceo kcfc` bjfn`pnb, tocp bjfn`pnb `j`oagc` cfct-cfct
ycae komutuhbca kca `j`pjrsocpbca sjecfc sjsuctu sjsuco kjaeca tnpob
prcbtobu` kosjfjaeecrcbca hcro otu.
k) \jtocp bjfn`pnb mjrtcaeeuaegcwcm ctcs
ctcs cfct-cfct ycae kopoagc`. Bjruscbca
ctcu hcf-hcf ycae `jayjmcmbca tokcb mjrduaesoayc cfct-cfct ycae kopoagc`
sjfc`c prcbtobu` mjrgcfca `jagcko tcaeeuae-gcwcm caeentc bjfn`pnb.
j) \jtjfch sjfjsco prcbtobu`, sj`uc cfct ycae kopoagc` hcrus kobj`mcfobca
kcfc` bjckcca mjrsoh.
=8
?) [uecs kcro fcmnrctnrou` `jaurut stcakcr O\N/OJL 359=: “ =99: sjsuco
kjaeca bn`pjtjasoayc komceo `jagcko kuc ycotu7 fcmnrctnrou` pjaeugo kca
fcmnrctnrou` bcfomcrcso.
39) \uctu prnkub ycae komutuhbca nfjh pohcb fcoa uatub bjpjatoaeca bjeoctca
sjpjrto pjrkcecacea, pjakokocba kca pjajfotoca `j`ofobo mubto mjrupc
sjrtodobct hcsof ugo/bcfomrcso.
33) \jkcae kjdoaoso kcro sjrtodobcso ckcfch prnsjs ycae koeuacbca nfjh pohcb
bjtoec uatub `j`mjrobca gc`oaca tjtufos mchwc suctu prnkub, prnsjs ctcu
gcsc `j`jauho pjrsycrctca ycae kotjtcpbca.
=?
Fcmnrstnrou` Yjaeugo
- Nrecanfjptob. - ]ckocso.
- @jbcaobc. - Cbustob.
- Bo`oc. - Tomrcso.
- Monfneo. - Nptob.
- Fostrob. - Ycacs.
- Yjaeugoca tokcb `jruscb. - Bfoaob.
Fcmnrctnrou` Bcfomrcso
- Cforca - \uhu
- Bjbjrcsca - Ucbtu kca Drjbujaso
- Bjfj`mcmca - Tomrcso
- Dfubs @ceajt - Tosbnsotcs
- @cssc - Tnfu`j
<9
MCM OOO
YJAZ[ZY
C. Bjso`pufca
Fcmnrctnrou` buftur gcroaeca ckcfch fcmnrctnrou` ycae koeuacbca bhusus
kcfc` pjajfotoca `jaebufturbca gcroaeca tu`muhca. Yjrtu`muhca jbspfca kcfc`
buftur gcroaeca kouschcbca kcfc` foaebuaeca ycae csjptob kca tjrbjakcfo.
Buftur gcroaeca tu`muhca ctcu [ossuj Lufturj `jrupcbca suctu `jtnkj
`jau`muh bj`mcaebca mceoca
mceoca tu`muhca, mcob mjrupc sjf, gcroaeca ctcu nreca
kcfc` bnakoso csjptol ctcu mjmcs kcro `obrn nrecaos`j. @jtnkj oao kofcbubca
sjlcrc oa votrn kcfc` tcmuae ctcu mntnf `jagcko tu`muhca ycae fjaebcp mceoca-
mceocaayc.
Fcmnrctnrou` Buftur Gcroaeca [u`muhca tjrkoro kcro rucaeca-rucaeca ycae
koposchbca mjrkcscrbca duaesoayc, ycotu ]ucae Yrcbtobu`, ]ucae Knsja, ]ucae
Cacfosc, ]ucae Yjrsocpca, ]ucae [rcasdjr/[cac`, ]ucae Buftur/Oabumcso kca
]ucae [o`mcae/Mchca Bo`oc. Yj`cbco fcmnrctnrou` hjakcbayc `j`chc`o tctc
fjtcb ctcu fcynut mcaeuaca fcmnrctnrou`. Yj`mcaeuaca suctu fcmnrctnrou` tokcb
kopjrlcycbca mjeotu scgc bjpckc sjnrcae crsotjbtur mcaeuaca.
@jaejtchuo Yjrcfctca kca mchca ycae Komutuhbca ko Fcmnrctnrou` Buftur
Gcroaeca [u`muhca.
Kcfc` `jfcbubca tctc bjfnfc/pjaejfnfcca fcmnrctnrou`, mjmjrcpc cspjb ycae
kopjrhctobca ycotu pjrjalcacca, pjactcca, Yjaeck`oaostrcsoca
Yjaeck`oaostrcsoc a kca Yjaec`caca,
pjrcwctca, kca pjaecwcsca
<3
KCD[C] YZ\[CBC
<=