ANSWERS TO ASPECTS OF ANAL (A) (B) (C) (D) (E) (A) (B) «cy (D) (E) (A) (B) (C) (D) (E) (A) (B) (©) (D) (E) (A) «ey «c) @) (E) (A) (B) (Cc) Ww) LYTICAL BIOCHEMISTRY & SEPA RATION TECHNIQUES rue False. Mastication is the physical st materials. True True True aring, ar chewing of food True True False. Sephades gel is used for sep: column or gel chromatograph False. Polyacrylamide get is used for electrophoresis of biomolecules as well as gel filtration, ation of biomolecules using ‘True True False. The mitochondrial fraction sediments at a speed higher than 12,0002. oluble fractions are separated at speeds higher than 100,000; ‘The isoelectric points of proteins are determined by titration and not centrifugation. ‘True True ‘True Truc True Troe False. Sellwanol?’s reaction is used to Identify keto sugars True . 313. ‘Scanned with CamScannerMW. th 13. 1 wy iy ay wy ay 1E) Ww an «) wy (Hh (A) (By (cy wy () (My ay (cy i) (ED «Ay (By «) Ww) (E) Ww (By (cy (Dy (E) (A) (By (cy «w) (E) (A) Jase, A speetrophotometer is better achaptedt for Uris. “[hisdireetly propartional (concent rai? alse readings mpurities such True True Truc True True True False Tru ‘True ‘True ‘Truc ‘True. Some adsorption is invalved, altho . ugh the maj i ce emcees zh the major driving force ‘True ‘True ‘True a4 ‘Scanned with CamScanner16. 7. 18. 19. ae wy) wn the ay ay wy ) tE) WA) ay (C) wD) 1B) (hy «thy wh wD (BE) (A) iB) (cy wy i) (AD a (c) ay an mw (By «) w) ws) um) (hr « iw The rev se is true ambers are used to vols ize the components to he | Motiseh test is used to identify earlooly rates. 1 is used For detection of proteins tiow of MRNAS ‘Scanned with CamScanner2 27, 29, 0. (A) (By «cy wo (E) fA) (B) ra) AD) (E) fA) (B) (C) (D) (E) (A) (B) (Cc) (D) (E) (A) (B) © © (5) (A) (B) (c) (D) (E) (A) (B) (C) (D) (E) (ay (B) (c) () (E) (A) «ay « (Dy (EY “yb BLO ENS Ne tae ify carson proper ‘True . Large molecules elute first duc to t to permeate the gel beads, while the smaller molecules penetrate the beads and are therefore retarded. ‘ulse. Elution volume is indirectly retated to molecular weight. Recall that large molecules elute at less volumes than small molecules. ‘True. ‘True False True False True ‘True True False ‘True ‘True ~ True ‘True False. ‘True True False. ‘True False. It depends on the biological properties of biomolecules. ‘True ‘True ‘True False. Il is a covalent linkage ‘True rare Alathalie rove (MY 3UCHIS True True True ‘Truc True False. Tog, LRH attachment to the gel is via covalest forces True False. Attinity chromatography separation ls performed using gels. 316 ‘Scanned with CamScanner