PhysioEx Lab Report

Exercise 12: Serological Testing

Activity 1: Using Direct Fluorescent Antibody Technique to Test for Chlamydia
Name: Manivannan Sharumathi
Date: 19 November 2021
Session ID: session-eda5a9eb-7b7e-4f5a-f2df-0badefb4ef1d

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 Which of the following has an epitope?

You correctly answered: an antigen.

2 Chlamydia trachomatis is

You correctly answered: a bacterium that reproduces inside its host cell.

3 The infectious form of Chlamydia that is spread from person to person is the

You correctly answered: elementary body that stains green in this activity.

4 The goal of the direct fluorescent antibody test is to test for the presence of

You correctly answered: antigen.

Experiment Results
Predict Questions
No Predict Questions
Stop & Think Questions

1 The source of the patient samples is

You correctly answered: epithelial scrapings.

2 What is the name for the specific part of the antigen that the antibody binds to?
:
 You correctly answered: epitope.

3 Drag the first slide (patient A) to the fluorescent microscope.

Count the number of elementary bodies you see through the microscope (recall
that elementary bodies stain green), enter the number of elementary bodies.

You answered: 0 elementary bodies.

4 Drag the next slide (patient B) to the fluorescent microscope.

Count the number of elementary bodies you see through the microscope (recall
that elementary bodies stain green), enter the number of elementary bodies.

You answered: 16 elementary bodies.

5 Drag the next slide (patient C) to the fluorescent microscope.

Count the number of elementary bodies you see through the microscope (recall
that elementary bodies stain green), enter the number of elementary bodies.

You answered: 1 elementary bodies.

6 Drag the next slide (positive control) to the fluorescent microscope.

Count the number of elementary bodies you see through the microscope (recall
that elementary bodies stain green), enter the number of elementary bodies.

You answered: 18 elementary bodies.

7 Drag the last slide (negative control) to the fluorescent microscope.

Count the number of elementary bodies you see through the microscope (recall
that elementary bodies stain green), enter the number of elementary bodies.

You answered: 0 elementary bodies.

Experiment Data
:
 Sample Number of Elementary Chlamydia Result
Bodies
Patient A 0 +
Patient B 16 -
Patient C 1 +
Positive Control 18 +
Negative Control 0 -

Post-lab Quiz Results

You scored 100% by answering 5 out of 5 questions correctly.

1 In the serology test just performed, the fluorescence is found on the

You correctly answered: antibody.

2 What would most likely happen if you forgot to fix the samples to the slide?

You correctly answered: They would all test negative because the washing steps
would remove the samples.

3 Which sample showed some residual nonspecific binding after the washing steps?

You correctly answered: patient C.

4 Which patient samples contained the Chlamydia-specific antigen?

You correctly answered: patient B.

5 How is chlamydia disease transmission prevented?

You correctly answered: abstinence or faithful, mutual monogamy.

Review Sheet Results

1 Describe the importance of the washing steps in the direct antibody fluorescence
test.

Your answer:
:
 The purpose of the washing steps to remove any non specific binding between
antigen and antibodies. The washing steps are to remove any non specific antigen
and antibodies that might occur.

2 Explain where the epitope (antigenic determinant) is located.

Your answer:

The epitope is a sub part of the antigen. Antigen are large. The epitome is small part
of the antigen where the antibody specifically binds.

3 Describe how a positive result is detected in this serological test.

Your answer:

A positive result is detected when fluorescently labeled antibodies bind to the

epitope regions found on the elementary bodies when they are present.

4 How would the results be affected if a negative control gave a positive result?

Your answer:

It would invalidate all of the results , could be a result of the enough washing.
: