Understanding COVID-19 Vaccine and

Therapeutic Development Research

SARS-CoV-2 Mechanism for Infecting Hosts

Spike (S) Protein

Coronavirus uses its spike (S) protein to
attach onto ACE2 receptors on human M Protein
cells. Targeting this virus-host receptor
interaction can prevent infection.
Genomic RNA

Targeting Virus-Host Receptor Recognition

to Block Viral Entry
S protein

Inhibitor

ACE2

 Developing antibodies, FAB, and scFv  The selection and characterization of

molecules that bind to viral spike proteins lead candidates based on accurate target
are at the forefront at inhibiting viral entry. binding kinetics, affinities and inhibitory
Similarly, small-molecule and peptides that potency is vital in the discovery workflow.
target the spike protein are also evaluated
as alternate therapeutic strategies.

Strategies for COVID-19 Vaccine Development

Infection Weakened
virus
The scientific community has made
tremendous efforts to understand the Inactivated
COVID-19 virus
disease, and unparalleled efforts are infection
ongoing to develop vaccines and Replicating
virus vector with
treatments. Studying antibodies Immune response V viral proteins
machinery
ac

generated from COVID-19 infections

c in

Non replicating
and vaccines can provide invaluable virus vector
e str at e g

with viral
information towards therapeutic proteins (AAV)
Immune response
antibody development and engineering.
Spike (S)
es
i

Kinetics, affinity and epitope Anti-coronavirus

antibodies are protein
interrelationships are important factors generated
DNA vaccine
in vaccine and therapeutic design. (with S gene)

mRNA vaccine
(with S message)

Octet® Systems: All-In-One Platform for

Therapeutic Investigation and Bioproduction
Octet® systems perform critical measurements with the speed and reliability
needed for investigating therapeutic strategies and producing novel medicine.

Evaluate Binding Kinetics and Affinities in Real-Time

Pick lead Biosensor Association Dissociation

candidates with 10-7
Binding (nm)

the best kinetics 10-6 0.1 pM

koff

and affinity 10-5

1 pM
S protein
properties 10-4 10 pM

10 nm 1 nm 100 pM
104 105 106 107
Time (s) kon

Assess Epitope Diversity Perform Titer,

and Coverage for Potency and Stability
Greater Success Measurements
Epitope binning assays help identify antibodies
that block the same epitope on a target antigen Biosensor
Standard
and are crucial when it comes to identifying or
Binding Rate

Strain specific
Unknown lot
antibody or
engineering mAbs with favorable kinetics and specific
affinity profiles. capture agent
17

41 5 78
88 72
92

82
51
70
8 10 62
Concentration
60 69
11 68
57 30 33
35 64

50 36
54

Identify antibody epitope  Fast at-line testing – Quantitate a 96-well

bins and their relationships plate for AAV titers within minutes.
 Three epitope binning assay formats to  Robust assays for antibody and virus titer
choose between. (ex. Influenza, AAV), potency and stability
determination in upstream and
 Different Bio-Layer Interferometry (BLI) systems
to meet your throughput needs and budget. downstream bioprocess development.

How Do Octet® Systems Help You Get Data Fast?

 Simple Dip and Read workflows help you develop assays quickly and get results within minutes.
 Minimal sample preparation time with analysis possible directly from crude samples.
 Range of available biosensor chemistries makes assay development easy.

Learn more about how Octet® systems are used

in COVID-19 research at sartorius.com/covid19

Specifications subject to change without notice. Copyright Sartorius Lab Instruments GmbH & Co. KG. For Research Use Only. 03 | 08 | 2021