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Case Studies in Chemical and Environmental Engineering 5 (2022) 100186

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Case Studies in Chemical and Environmental Engineering


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and-environmental-engineering

Biotechnological advancements and challenges in textile effluents


management for a sustainable bioeconomy: Indian case studies
Anita Srivastava a, *, Sheetal Bandhu b
a
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India
b
Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India

A R T I C L E I N F O A B S T R A C T

Keywords: The textile industries role on imminent hazardous environmental impact has global attention. The remediation of
Textile waste effluent textile effluent is often challenging because of the presence of a wide variety of pollutants like dyes, acids, bases,
Biological processes metal salts, and microorganisms. The use of biological recycling methods are contemplated to mitigate the
Recycling
hazardous pollutants from textile effluent to reduce environmental pollution, threatening the sustainability of
Case studies
Legislations
living organisms. This review aims to furnish an insight into the recent advancements and challenges in
biotechnological processes focusing on economic and cutting-edge technologies in the treatment of textile ef­
fluents. Recent trends using integrated biotechnological approaches and bioprocess engineering represent stra­
tegic opportunities for economic textile effluent management. Studies on advanced in-silico and multiomics
(proteomics, genomics, metabolomics) approaches have been highlighted. This review also identifies the gaps
and issues in effluent treatment operations. Several case studies on waste effluent treatment and recycling
practices in India are also reported. Important legislation and laws for regulating and controlling textile waste
effluent are also described.

1. Introduction carcinogenic effect on public health [8]. In India, the majority of textile
industries are located in Tirupur and Karur in Tamil Nadu, Kanpur in
Sustainable development is the vital element of the modern economy Uttar Pradesh, Ludhiana in Punjab, and Surat in Gujarat [9]. Technical
as it switches from linear economy to circular bio-based economy. improvisation is the need of the hour for the pre-processing steps, to
Globally, exploration and application of the latest trends in biotech­ reduce the use of chemicals and cost of treatment or disposal. This article
nology for waste treatment represent a sustainable environmental focuses on the advancements in the treatment of textile effluent using
ecology and circular bioeconomy [1]. India is contributing to a target of biological, enzymatic, microbial, genetically modified microorganisms
$100 billion bioeconomy by 2025 with annual growth of 20% [2]. nanotechnology, and membrane bioreactors (Fig. 1). Microbial assisted
Among all crucial contributors in the Indian economy textile industry bioremediation using advanced multiomics tools (genomics, tran­
possess a pivotal share and is the second largest employment generator scriptomics, proteomics, and metabolomics) and in silico approaches
after agriculture. Indian textile industry is estimated to reach US$209 have also been discussed. Recent studies on Bioinformatics and Molec­
billion by the end of 2021 [3]. Industrial textile processing from fiber to ular docking tool are also shown [10,11]. The in silico methods are being
fabric comprises pretreatment (sizing, scouring, and desizing), dyeing, applied in many fields for toxicity evaluation and biodegradation of
printing, and finishing operations requiring toxic chemicals, stabilizers, environmental pollutants. This review provides insight on the effluent
finishing agents during different stages [4,5]. These production pro­ generation during different textile processing steps, highlighting various
cesses use ample amounts of water and a wide range of different syn­ advanced biological treatment technologies, their advantages, and
thetic dyes (azo, directive, acidic, and basic) discharged as effluent, limitations for the better management of textile industries (Table 1).
adding to environmental hazards with large consumption of energy and Few case studies with the recent biotechnology-assisted treatment of
water [6,7]. Microbial communities and water bodies are tremendously textile waste effluent in Indian textile industries are discussed. It also
affected by the textile effluent due to the reduced availability of sunlight presents the key issues and challenges in Indian policies and regulations
and dissolved oxygen and its toxic, mutagenic, cytotoxic and during textile processing for waste management.

* Corresponding author.
E-mail address: anitasri27@gmail.com (A. Srivastava).

https://doi.org/10.1016/j.cscee.2022.100186
Received 30 September 2021; Received in revised form 18 January 2022; Accepted 30 January 2022
Available online 1 February 2022
2666-0164/© 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license
(http://creativecommons.org/licenses/by-nc-nd/4.0/).
A. Srivastava and S. Bandhu Case Studies in Chemical and Environmental Engineering 5 (2022) 100186

2. Technological status: biotechnological tools in effluent another medium. Despite several advantages, the process has certain
treatment and recycling in India limitations like being restricted to biodegradable compounds only, more
time consuming. Future research requires the development of advanced
According to the US EPA, an average of a minimum of 40 L of bioremediation technologies for non biodegradable dyes and complex
freshwater is required for coloring 1 kg of cloth, which may vary ac­ pollutants treatment.
cording to the dyeing process of textile material. The wastewater Microbial fuel cells technology employing different microorganisms
released consists of different suspended mineral salts and oils, metals may be proven as a promising technology that converts chemical energy
and their complexes and dyes, some non-biodegradable products if un­ from the degradation of pollutants into electrical current, using some
treated will pollute the rivers and sea after discharge. Annually, ~830 electrodes for the effective treatment of textile wastewater. This tech­
million m3 of groundwater is used, and ~640 million m3 of wastewater nology is being globally studied as a multidisciplinary discipline that
is discharged [12,13]. There are significant opportunities in effluent integrates microbiology, electrochemistry, material science, and other
recycling and waste acquired bioeconomy, the country has the potential fields to address environmental and fuel-related issues. The bio­
to transform it into a sustainable business opportunity. Biological pro­ electrochemical mechanism of electroactive bacteria is being researched
cesses such as bioremediation and its integration with advanced as an effective strategy in wastewater treatment, bioelectricity produc­
computational tools provide a distinct edge to address the present tion, and resource recovery using this technology. This method has
environmental challenges and are considered to be unostentatious and several limitations, including low electricity production, high cost, un­
phenomenally effective in eradicating various biohazardous from textile steady kinetics and, environmental toxicity [20]. Another effective
effluents [14]. approach offering a potential treatment is using genetically modified
organisms. Researchers have developed different transgenic strains for
2.1. Bioremediation effluent treatment.

Bioremediation-assisted textile effluent treatment has been devel­ 2.2. In silico and multiomics approaches
oped for relatively cleaner processing. These methods are robust due to
their versatility and molecular diversity, the most effective alternatives In addition, an emerging alternative computational method, in silico
as compared to physical and chemical methods with economical oper­ approaches is being applied for the bioremediation of complex pollut­
ating costs and production of non-toxic and harmless products. The ants from the textile effluent. This bioremediation approach relies on
treatment processes involve a wide array of microorganisms or their diverse areas of science including bioinformatics, computational
enzymatic machinery [15–17]. Recently research has been developed biology, molecular modeling, genomics, proteomics, molecular dy­
using microbial consortia for effective textile effluent management. In a namics simulation, and algorithm for the prediction of pathways [21].
microbial consortium, an individual strain may attack dyes molecules at Recent studies also show the application of multiomics in designing the
different positions. Moreover, the degradation products, which strategy for microorganism assisted remediation to rehabilitate the
appeared due to the metabolic activity of one strain, may be utilized as pollutant contaminated environment and water [10,22]. The in silico
substrate by another [18]. The microorganisms such as bacteria, fungi, method, docking process astute to study the biodegradation of pollut­
yeast, and algae have been widely used by various researchers in textile ants by microorganisms, in addition, provides an insight to understand
wastewater treatment [Fig. 2]. These microorganisms explore different the biochemical and molecular reactions taking place inside the
biochemical pathways and biosorption processes to degrade, decolorize, microorganism during the bioremediation process. Metabolic models
detoxify and mineralize a cavalcade of wastewater pollutants by using and genome-sequencing integrate all features of an organism by
their different metabolic pathways and biosorption processes [19]. The combining genome explanation and biochemical understanding to
ambient parameters can be changed to stimulate microbial development create a mathematical model [23]. Bioinformatics and molecular
and hence increase the microorganisms’ degrading efficiency for an docking are widely used tools for predicting susceptibility of dye
effective bioremediation procedure. Furthermore, the procedure is degradation using oxidoreductive enzymes. Dye and enzyme reaction
eco-friendly, simple, labor-intensive, and sustainable. It also ensures the analysis provides the specificity of substrates and predicts the mecha­
eradication of toxins from the environment without being transmitted to nism of degradation [24]. Membrane technologies may also be a robust

Fig. 1. Textile effluent treatment and recycling using integrated biotechnological approaches.

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A. Srivastava and S. Bandhu Case Studies in Chemical and Environmental Engineering 5 (2022) 100186

method for effluent management. Several emerging membranes such as for wool fabrics. The treated fabrics showed redder and yellower regions
ultrafiltration, microfiltration, nanofiltration, and reverse osmosis are of color irrespective of the variable temperatures with ultraviolet pro­
widely used in the purification and desalination of dyes in wastewater tection and resistance property against Escherichia coli and Staphylo­
[25]. These membranes are sturdy in the removal of color, suspended coccus aureus [29].
solids, and organic salts from textile wastewater. The membrane’s Nirena and Jigishab (2011) investigated the treatment of red dye,
filtration function separates particles from liquid, making the process containing synthetic textile wastewater using an aerobically run
more efficient. Gunes and Gonder evaluated the efficiency of the hybrid continuous flow mode submerged membrane bioreactor (SMBR), with
system (electrocoagulation-nanofiltration-reverse osmosis) for biologi­ different hydraulic retention times and an increase in transmembrane
cally treated textile effluent. The membrane performance and fouling pressure from 0.29 to 0.58 bar during each run. The removal rate for
behaviors of the hybrid treatment processes were evaluated [26]. BOD, COD, and color was 93.69% 92.33%, and 91.36%, respectively. A
Membrane bioreactors have various advantages, including high effluent stable flux and fouling were prevented by covering the membrane with a
quality, flexibility, low sludge production, enzyme washout prevention, wire mesh cage and routine cleaning was performed. The findings
and low environmental impact. Different kinds of bioreactors like airlift revealed that SMBR is efficacious for treating synthetic textile waste­
bioreactors, fluidized bed bioreactors, fixed bed or packed bed bio­ water [30]. Kadam et al. (2018) reported the effective use of a floating
reactors are also used as attractive approaches for wastewater treat­ photo bed system based on the plant consortium of Chrysopogon ziza­
ment. These bioreactors are highly advantageous because of their simple nioides and Typha angustifolia for the removal of Scarlet RR Dye and
design, low cost, continuous operations, uniform mixing, and better treatment of textile effluent by using the plant consortia linked to mi­
temperature control [27]. crobial fuel cells resulting in a reduction of color, biological oxygen
demand, chemical oxygen demand total suspended solids and total
3. Textile effluent treatment in India: case studies dissolved solids [31].
Two non-adapted bacteria, Aeromonas hydrophila MTCC 1739 and
Globally, a tremendous amount of research is being carried on the Lysinibacillus sphaericus MTCC 9523 were investigated for a textile dye
remediation of textile effluent for reuse. A group of researchers inves­ decolorization (azo dye Drimaren Red CL-5B). An advanced Biosolve IT
tigated the impact of treated textile wastewater for irrigation, the co­ software was adopted to calculate docking score, identification of active
conut fiber was used as a natural biomaterial for mineralization of site residues, and molecular interactions between dye and bacterial
methyl orange containing wastewater in an integrated biofilm biore­ laccase and azoreductase. It was suggested that both bacterial strains
actor, Pseudomonas putida showed a strong ability to decolorize methyl had the potential for decolorization and biodegradation of azo dye
orange under aerobic conditions at ambient temperature and pH, under containing textile wastewater. Thus, an advanced approach was vali­
static state. The treated water showed better results as compared to dated, for an in silico preliminary screening and bacterial dye degra­
textile water used for irrigation in terms of nutritional profile [28]. dation, for an economical, eco-friendly, and time-efficient
Another study suggests the utilization of chickpea outer husk exuber­ bioremediation for reprocessing of treated water [32]. The researchers
antly plentiful agro-industrial processing remnant in India for the devised a synergistic plant-microbe system, to evaluate the complete
extraction of textile grade dye to transmit color to cotton, wool fabric, degeneration of a methyl red azo dye, operated in an anaerobic-aerobic
silk without any auxiliaries. Good dye uptake was seen in the chickpea sequential mode. The breakdown products of methyl red (N, N-dime­
husk extract-treated cotton, silk, and wool fabrics. The highest color thyl-p-phenylenediamine, and 2-aminobenzoic acid) were analyzed. The
depth (K/S) of 60.4 and a total color value of 3.4 was produced at 90 ◦ C intensified dye degradation was observed by the vetiver-microbe

Table 1
Trending integrated biotechnological approaches and their outcomes in textile effluent treatment.
Emerging trends Advantages Challenges Future scope References

Bioremediation Potential to degrade various synthetic dyes Limited knowledge about Potential to be more effective with [7,8,40,
and heavy metals, low energy consumption metabolic and degradation interdisciplinary approaches 43]
as compared to incineration and landfilling, pathways
cost-effective, and easily accepted by
regulatory authorities
In silico approaches A modelling tool, provides an understanding Availability of limited The integration of advanced biological, [11,24,41,
(bioinformatics, molecular of biochemical and molecular pathways resources, limited database toxicological and microbial database may be 42]
docking), multiomics during the bioremediation process restricted to few compounds integrated as a single tool to enhance the
(genomics, proteomics, computational techniques for improved
metabolomics) results
Microbial fuel cell technology Production of bioenergy, electricity, reduced High operation cost, less Advanced research may lead to low-cost [31]
CO2 emission, mineralization of pollutants efficient, low production rates power generation and renewable energy
production
Enzymes and whole cell Degradation of complex dyes and utilization Limitations in terms of Technological advancements in synthetic [24,37,40]
biocatalysts of byproducts substrate and acceptor biology and metabolic engineering, efficient
tolerances, stability, low immobilized biocatalysts in this area
catalytic turnover, and end
product inhibition
Metagenomics and gene editing Offer a unique untapped opportunity for the Limited knowledge of factors The combined approach of DNA, RNA, and [45]
bioremediation, identification of the controlling growth, pathways, protein for analyses of a microbial
potential gene, encoding for enzymes and microbial metabolism. community may develop an understanding of
functions in reducing the toxicity of wastes Safety concerns, ethical a relationship with the environment and their
generated. Identifies and targets several acceptance functions. Identifying novel pathways and
specific sites within the genome using role of protein engineering for better tool
CRISPR, ZFN etc. development in bioremediation
Membrane bioreactors Highly efficient by using combined microbial Fouling of membrane, high Technological developments in mitigation of [33,38]
and membrane filtration processes plays a operation cost fouling of the membrane
major role in the degradation of color
compounds and mineralization of textile
wastewater

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A. Srivastava and S. Bandhu Case Studies in Chemical and Environmental Engineering 5 (2022) 100186

Fig. 2. Overview of generation of textile effluent and treatment using bioremediation technologies.

system. The plant-microbe system was most efficient for the removal of procedure resulted in 90% efficient laccase immobilization with more
aromatic amine which affected the phytotoxicity during seed germina­ than 70% stability after 5 months at 4 ◦ C. The results led to effective
tion and teratogenicity in zebrafish development in applied treated immobilization and dye decolorization [40]. Researchers also reported
water [33]. Another study was performed on Pseudomanas aeruginosa the use of in silico studies using Pseudomonas stutzeri TS44 arsenite ox­
SVM16 mediated decolorization of azo anthraquinone dyes, utilizing the idase, a bacterial enzyme, to understand bioremediation for the elimi­
dyes as substrates and supplemented with jack fruit seed powder as nation of toxic arsenic, leading to an economical and eco-friendly
effective co-substrate for bio-decolorization [34]. Mishra et al. (2020) approach. Following sequence analysis, models were generated using a
also reported an advanced approach, the use of microbial fuel cells variety of homology modeling methods. Molecular docking was studied
(MFCs) for hybrid (mixed azo orange 16 (RO16) and red 21 (RR21) for the binding interaction of arsenite oxidase with arsenite [41].
dyes) wastewater treatment along with energy recovery using Pseudo­ Another group of researchers worked on six different dyes as potential
monas aeruginosa 23N1 using dye as substrate and resulting in targets for degradation by laccase and azoreductase using Aeromonas
bio-energy by using the organic compounds formed as products. The hydrophila with Lysinibacillus sphaericus respectively, implementing in
bio-energy formed may vary depending upon the concentration of silico docking tool (BioSolveIT-FlexX). Azoreductase used a ping-pong
inorganic or organic compounds, physicochemical composition mechanism to break azo bonds, whereas laccase used a non-specific
including wastewater [35]. free radical method to decolorize dyes [24]. Chakdar et al. (2021)
The researchers reported the degradation of a sulfonated azo dye highlighted the potential of cyanobacteria for heavy metal bioremedi­
(Acid Fast Yellow MR) by a newly identified Aeromonas hydrophila SK16 ation and outlooked a critical insight on their genomic and proteomic
bacterial strain from textile effluent polluted soil. The oxidoreductase regulation of multiple tolerance mechanisms [42] These approaches
was involved for the dye decolorization revealed by Fourier Transform may add new dimensions for implementing computational tool, in silico
Infrared Spectroscopy showing the preference of amine and sulfur di­ approaches for improving bioremediation method for eradication of
oxide bonds in the dye breakdown [36]. Another study on decoloriza­ pollutants from textile effluents.
tion of the dye was reported by Prabhakar et al., in 2019, using an
isolated bacterial Nesterenkonia sp., the study was executed in simulated 4. Challenges in textile effluent treatment
and real textile wastewaters using starch as a carbon supplement. The
microbe efficiently biodegraded the dye as well bioremediated the toxic Textile industry effluent management is demanding careful consid­
metal Cr (VI), moderated the pH from wastewater during treatment eration due to its serious environmental consequences. The biochemical
[37]. The author devised an anaerobic biological reactor merged with an properties of textile waste effluent like higher chemical oxygen demand,
activated carbon filter and ultra-filtration unit termed as AN-ACF-UF heat, pH, color, non-degradable dyes, and metal ions are imposing dif­
process, containing a microbial consortium for the decolorization of ficulties in treatment. Textile industries are confronting serious chal­
textile effluent at an on-site industry. The process involved the treatment lenges due to the lack of advanced processing and effluent treatment
of industrial effluent containing dye and pre-treatment range (PTR) in a technologies at pilot and industrial scales. Various traditional physico­
specific ratio using the patented method. The system was efficient in chemical or biological as well as emerging advanced methods are
recovering salt with a high concentration of sodium with a zero liquid available but, currently, there are no proven efficient treatment methods
discharge system [38]. Raj et al. (2021) suggested the two-step process that could overcome drawbacks like high energy consumption, oper­
for remediation of textile effluent for water reuse using Escherichia fer­ ating cost, long treatment time, and production of hazardous
gusonii, followed by activated charcoal treatment. The bacterium byproducts.
potentially reduced the color, chemical oxygen demand, total dissolved Bioremediation is widely applied in the textile industry yet, the
solids, bicarbonates, sulphates, and chlorides in a significant amount resulting products of azo dyes are carcinogenic and mutagenic. Total dye
[39]. Chhabra et al. (2015) demonstrated Cythus bulleri immobilized degradation is still lacking due to the unavailability of robust technology
laccase mediated azo dye acid red 27 decolorization using batch for effluent treatment [43]. Microbial fuel cell technology have also
(90–95%) and continuous packed bed column (nearly 90%). The emerged as a promising bioremediation solution; however, efforts are

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A. Srivastava and S. Bandhu Case Studies in Chemical and Environmental Engineering 5 (2022) 100186

needed to overcome their limitations, such as limited electricity pro­ techniques are capable of revolutionizing the biological treatment of
duction and high cost. Genomics and proteomics dependent bioinfor­ contaminated environments by allowing highly sensitive characteriza­
matics holds great assurance as a tool to address enduring queries tion and functioning of the yet-uncultured microorganisms. To gain an
regarding the molecular mechanisms about biodegradation pathways understanding of intricate in situ bioremediation processes, monitoring
and has shown its novel potential in bioremediation processes. However, techniques, enzyme probes, genomics, transcriptomics, proteomics, and
the scarcity of the resources required for the bioremediation processes metabolomics provide unique insights into the important microbial re­
makes it less effective. Despite certain advantages, the membrane-based actions [10]. The implementation of the in silico approach reduces time
treatment technologies used for separation also deserve special research. and basic laboratory experiments and is important in the prediction of
The membrane bioreactor performance has improved dramatically in degradation pathways. Bioinformatic tools and software aid in a time
recent years as a result of many improvements, but membrane fouling is and cost-effective screening of the targets for bioremediation compared
a serious issue to be addressed for enhancing the efficiency, efficacy, and to other methods. The biological processes researched for this area seem
cost-effectiveness of the process. Membrane development with good to be quite effective and meet compliance with current legislation.
rejection rates, antifouling properties, and high throughput should be Emerging technologies described by various researchers for the degra­
focussed. Airlift bioreactors, fluidized bed bioreactors, and fixed bed or dation and detoxification of effluent are promising and have the po­
packed bed bioreactors have also certain limitations of mass transfer, tential for economic and environmental sustainability. It is suggested to
erosion, and large reactor volume which are to be overcome. Moreover, evaluate on a pilot scale, economical assessment of different technolo­
the technologies developed so far are still processed at lab-scale and gies as well as cost analysis of the real application for further studies.
significant efforts are required to scale up for full-fledged industrial Current trends in effluent treatment consider the investigation of the
operations. The technologies should be applied at pilot or industrial mixed textile effluent from the plants without giving a prior consider­
scale. Textile effluent treatment plants are considered as an attainable ation to the treatment of the effluent produced at different units of the
solution for effective effluent treatment. Since treatment plants have industry. Since the effluent consists of a variety of chemical components
lack of technical knowledge and are least updated with technological and auxiliaries, the separation of different streams according to biode­
advancements. A collaboration with academia should be encouraged to gradability should be performed. On-site wastewater treatment and
select an adequate process for a clean and green and sustainable water recycling are highly recommended.
environment.
7. Conclusions
5. Legislations and regulations for textile effluent management
in India Textile wastewater recycling has significant potential for a sustain­
able bioeconomy. A staggering amount of interdisciplinary advanced
The Indian government took initiatives and started various schemes research is required to address the challenges, especially focusing on
for Research and Development for the textile industry under the 12th membrane engineering, genetic engineering, multiomics, in silico ap­
five plan (2014–2015 to 2018–2019) with a financial budget of Rs. 149 proaches, and synthetic biology to customize the microbial metabolism
crore. The Ministry of Textileshas been vigorously supporting Textile for bioremediation as an effective tool in textile effluent management.
Research Associations, covering the entire spectrum of the sector tar­ Furthermore, strong measures are required to prevent the release of
geting green technologies and circular economy. pollutants into the aquatic environment, development of water man­
Various committees have been constituted in India since 1974 (water agement policies and strategies should be stringently implemented.
act) to impose judicial conciliation to curb water pollution. However, Noteworthy, steps should be taken up by the government, scientific
there are no specific environmental laws for the textile sector alone. fraternity, and industries to work on the potential opportunities in this
There are industry-specific standards, to be followed by the textile in­ area. Business opportunities in textile effluent treatment can also be
dustries while operating an industrial unit. The regulatory authorities created in collaboration with the government, providing financial aids
for textile waste management are monitored by the Ministry of Envi­ under the ‘Swachh Bharat’ program in India. Start-up companies have
ronment and Forests (MoEF), the State Pollution Control Board (CPCB), tremendous scope and opportunities in waste recycling and manage­
and the Central Pollution Control Board (CPCB). In 2014, national law ment. An independent authority is demanded proper regulation of
and policy began to move towards the circular economy. At the end of textile wastewater, which may lead to innovation in recycling processes.
2015, the Ministry of Environment, Forest and Climate Change Waste management companies should be promoted financially from the
(MoEFCC) issued a Draft Notification for Amendment Rules on Stan­ tax derived from the polluters.
dards for Effluent from the Textile Industry [44]. As a result, the
installation of wastewater treatment plants and the improvement of
resources became obligatory for the pollution drivers. Despite environ­ Declaration of competing interest
mental legislation, pollution control is limited due to substandard
monitoring, enforcement of policies and regulations by the management The authors declare that they have no known competing financial
bodies, slow response of courts in enforcing actions, the financial interests or personal relationships that could have appeared to influence
constraint of the Boards, and low penalties for non-compliance. the work reported in this paper.

6. Prospects and recommendations Acknowledgments

Innovative waste prevention strategy should be focussed to prevail The authors devote this paper as the contribution in research for
over these challenges. Various emerging technologies like phytor­ biotechnological textile effluent recycling for sustainable bioeconomy
emediation and constructed wetlands could be adapted as eco-friendly and this paper is not a part of any work, financially aided from any
tools for bioremediation. Membrane technology and membrane bio­ funding bodies.
reactors could be substantially useful for achieving good quality of
treated water for recycling and irrigation purposes. The combination of References
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