A PROJECT REPORT

ON

Production and Characterization of α Amylase Enzyme by

Bacillus subtilis ATCC 231

Submitted in partial fulfillment for the award of the degree

of
Bachelor of Science in Bioinformatics

By
Mr. Jadhav Anil Kishanrao

Under the guidance of

Ms. Asmita K. Kulkarni

Mahatma Gandhi Mission’s

College of Computer Science & IT,
Department of Biotechnology & Bioinformatics
MGM Campus, Nanded-431605

Affiliated to

Swami Ramanand Teerth Marathwada University,

Nanded-431606

2020-21
 Mahatma Gandhi Mission’s
College of Computer Science & IT,
MGM Campus, Nanded 431605

Certificate
This is to certify that the project entitled “Production and Characterization of α
Amylase Enzyme by Bacillus subtilis ATCC 231” by Mr. Jadhav Suresh
Kishanrao (Exam Seat No………….) submitted in partial fulfillment of the
requirements for award of degree of Bachelor of Science in Bioinformatics of
Swami Ramanand Teerth Marathwada University, Nanded 431606 during the
academic year 2020-21, is a bonafide record of work carried out under my
guidance and supervision.

Ms. Asmita K. Kulkarni

Guide

Dr. Makarand N. Cherekar Prof. Shirish L. Kotgire

Vice Principal & Head Principal

Date: / /
 Declaration

I hereby declare that the Project report entitled “ Title……….” has been
completed in Department of Biotechnology & Bioinformatics, MGM’s College of
Computer Science & IT, Nanded and Submitted to Swami Ramanand Teerth
Marathwada University Nanded, under the guidance of Ms. Asmita K. Kulkarni
for the award of degree of Bachelor of Science in Bioinformatics.
This report comprises only my original work and has not been submitted for any
other degree/ diploma to any university/ institute. Due acknowledgement has been
made in the text to all other material used

Mr. Jadhav Anil Kishanrao

Candidate

Date: / /
Place: Nanded
 Acknoweldgement

My heartfelt thanks to college Principal Dr. Shirish L. Kotgire whose valuable

support lead me to learn much more than the curriculum. I am elevated to avail
myself of this opportunity to express my deep sense of my gratitude and
indebtedness toward Dr. Makarand N. Cherekar Head of Department of
Biotechnology & Bioinformatics and my guide Ms. Asmita K. Kulkarni whose
constant encouragement and guidance has helped throughput the course of this
dissertation work.

I am also indebted to S.R.T.M University for keeping this dissertation as a part of

syllabus, which gave me great opportunity and knowledge.

My heartfelt thanks to Dr. Makarand N. Cherekar Head of Department og

Biotechnology & Bioinformatics and my guide Ms. Asmita K. Kulkarni whose
constant encouragement and advise with valuable suggestion. I have a special
thanks to all the faculty member of department for their constant encouragement
and support throughout this duration. I am also greateful to librarians, for their help
during my project work.

Mr. Jadhav Anil Kishanrao

Candidate

Date : / /
Place: Nanded