実体顕微鏡総合カタログ

Stereo Microscopes

Stereo Microscopes
100-8331 東京都千代田区丸の内3-2-3 富士ビル
NIKON CORPORATION
Fuji Bldg., 2-3, Marunouchi 3-chome, Chiyoda-ku,
Tokyo 100-8331, Japan
www.nikon.co.jp/ www.nikon.com/

NIKON CORPORATION
Fuji Bldg., 2-3, Marunouchi 3-chome, Chiyoda-ku,
100-8331 東京都千代田区有楽町1-12-1（新有楽町ビル） Tokyo 100-8331, Japan
www.nikon.co.jp/ www.nikon.com/

NIKON CORPORATION
Fuji Bldg., 2-3, Marunouchi 3-chome, Chiyoda-ku,
100-8331 東京都千代田区有楽町1-12-1（新有楽町ビル） Tokyo 100-8331, Japan
www.nikon.co.jp/ www.nikon.com/
 Index
Stereo Microscopes
• SMZ25, SMZ18‥ ‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 4
• SMZ1270/1270i, SMZ800N‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 8
• SMZ745/745T‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 12
• SMZ445/460, SMZ-2‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 13
Accessories (for SMZ1270/1270i, SMZ800N,
SMZ745/745T, SMZ445/460, SMZ-2)
• Objectives, Auxiliary Objectives‥‥‥‥‥‥‥‥‥‥‥‥‥ 18
• Nosepieces, Tubes, Eye-level Riser,
Intermediate Tubes‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 19
• Stages, Observation Attachments‥‥‥‥‥‥‥‥‥‥‥‥ 20

The Next Revolution in Microscopy

• Illumination Systems‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 21
Accessories (for SMZ25, SMZ18)
• Stands‥ ‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 22
• Base Unit, Focus Unit, Stand/Focus Mount‥ ‥‥‥‥‥‥ 14
• Universal Table Stands/Focusing Mounts‥ ‥‥‥‥‥‥‥ 23
• Objective, Tubes, Nosepiece/Focus Mount Adapter,
Stage‥ ‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 15 Specifications/System Diagrams

A Giant Step Forward in Stereo Microscopy • Controller, Darkfield Observation Accessory, Polarizing
Observation Accessory, Epi-fluorescence Set‥ ‥‥‥‥‥ 16
• Fiber Illuminator Set, Coaxial Illuminator,
• System Diagrams (SMZ25/18)‥ ‥‥‥‥‥‥‥‥‥‥‥‥ 24
• Specifications (SMZ25/18)‥ ‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 25
• System Diagrams
Ring LED Illuminator‥ ‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 17
(SMZ1270/1270i/800N, SMZ745/745T)‥ ‥‥‥‥‥‥‥‥ 26
• Specifications‥ ‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 28
Nikon offers a broad range of stereo microscopes and accessories, including a research stereo
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SMZ1270/ SMZ445/
SMZ25 SMZ18 SMZ800N SMZ745/SMZ745T SMZ-2
1270i SMZ460

Optical system Parallel-optics type Greenough type Optical system

NEW NEW

Zoom ratio 25:1 18:1 12.7:1 8:1 7.5:1 4.4:1 / 4.3:1 5:1 Zoom ratio

Zooming range 0.63-15.75× 0.75-13.5× 0.63-8× 1-8× 0.67-5× 0.8-3.5× / 0.7-3× 0.8-4× Zooming range

Total magnification*1 3.15-945× 3.75-810× 3.15-480× 5-480× 3.35-300× 4-70× (8-35X)/ 4-120× Total magnification*1
(with standard set*2) (6.3-157.5X) (7.5-135X) (6.3-80X) (10-80X) (6.7-50X) 3.5-60× (7-30X) (8-40X) (with standard set*2)

Working distance*3 60mm 60mm 70mm 78mm 115mm 100mm 77.5mm Working distance*3

Image capture ○ ○ ○ ○ ○ (SMZ745T) ― ― Image capture

System expandability ○ ○ ○ ○ ― ― ― System expandability

Embedded use ― ― ○ ○ ○ ○ ○ Embedded use

*1 Depends on the combination of eyepiece and objective lens *2 With a 10x eyepiece and a 1x objective
*3 With a 1x magnification without auxiliary objective
2 3
Parallel-optics type
Research Stereo Microscope

Remarkable resolution and the world’s widest zoom range

Dynamic zoom ratio of 25:1 SMZ25

Evolutionary stereo microscope 0.63x

2x
Nikon has developed an all-new stereo microscope that features a large zoom ratio of 25:1, high resolution and 5x
exceptional fluorescence transmission capability. The new stereo microscope meets the increasing needs for imaging
15.75x
systems that span spatial scales from single cells to whole organisms.

World’s widest zoom range and highest

resolution for a stereo microscope
• First stereo microscope to offer a 25:1 zoom range (SMZ25)
• Both eye paths boast numerical apertures (NA) of up to 0.156,
using the SHR Plan Apo 1x objective and SMZ25
Bright, high-contrast fluorescent images
• Fly-eye lens ensures uniform brightness over the entire field
of view even at the lowest magnifications
• Breakthroughs in optical design mean significantly improved
signal to noise ratio and crystal clear fluorescent images
An innovative optical system known as “Perfect Zoom Optics” offers
the world’s first zoom ratio of 25:1 (zoom range: 0.63x - 15.75x*;
*as of May 2013). The SMZ25 can seamlessly capture the entire
dish while simultaneously delivering microscopic details.

Automation and digital imaging Easy to use

Auto Link Zoom (ALZ) supports seamless viewing at different scales SMZ25

• Motorized focus and zoom operation (SMZ25) • User-friendly remote control (SMZ25)
• Imaging Software NIS-Elements enables the use of multiple • Easy-to-operate slim LED DIA base with OCC illumination ALZ automatically adjusts the zoom
imaging, processing and analysis modalities, including factor to maintain the same field of
• Wide range of illuminators and accessories that
z-stack capture, time-lapse imaging and EDF image view when switching objective lenses.
accommodate a variety of observation methods
generation This function enables seamless
switching between whole organism
imaging at low magnifications
and detailed imaging at high
magnifications.
SHR Plan Apo 0.5× SHR Plan Apo 2×
zoom 6× zoom 1.5×

Maintains FOV at total magnification of 3×

Motorized zoom model with the Manual zoom model providing advanced Superior resolution never before seen on a stereo microscope SMZ25 SMZ18
highest zoom ratio and resolution in optical performance and incredibly bright
the SMZ series fluorescence at an attractive price
Newly developed SHR (Super High Resolution) Plan Apo series Comparison of resolution and color aberration by
Model SMZ25 SMZ18 objective offers a resolution of 1100LP/mm (observed value, using resolution chart
Type Motorized zoom Manual zoom SHR Plan Apo 2x at maximum zoom). The 0.5x, 1x, or 1.6x lower SMZ25 Conventional model
magnification objectives deliver a bright field of view and brilliant
Observation Brightfield/Darkfield/Fluorescence/Simple polarizing
images with true-to-life colors.
Zoom ratio 25:1 18:1

0.75x - 13.5x
Magnification range 0.63x - 15.75x
(with 0.75/1/2/3/4/5/6/8/10/12/13.5x click stops)

Maximum magnification 315x*1 270×*1

Maximum FOV ø70 mm*2 ø59 mm*2

Maximum NA of 0.312*3 0.3*3

*1: Using SHR Plan Apo 2x/ C-W10xB *2: Using SHR Plan Apo 0.5x/ C-W10xB *3: Using SHR Plan Apo 2x

4 5
Parallel-optics type
Bright, high-contrast fluorescent images SMZ25 SMZ18 Improved observation efficiency
Enhanced brightness and uniform illumination in a low magnification range Easy-to-use OCC illumination SMZ25 SMZ18

The SMZ25 series is the first stereo microscope in the world to use a fly-eye lens on The new LED DIA Base with built-in OCC illuminator generates
an epi-fluorescence attachment. This ensures bright, uniform illumination even at low New epi-fluorescence attachment minimal heat, consumes little power and has a long life. The Comparison images (film)
magnifications across a large field of view. Lens Lens illuminator also enhances the contrast of uneven surfaces, such as
those of film.
Improved S/N ratio and crystal clear fluorescent images thanks to Light source
an improved optical system

Nikon has succeeded in improving the signal and reducing noise in fluorescent
images by using a short-wavelength, high-transmission Fluor lens. This enables Fly-eye lens uniformly
illuminates the entire field Conventional diascopic illumination OCC illumination
observations of cell division and samples with weak fluorescence, both of which are of view
difficult using conventional stereo microscopes.

What is OCC illumination?

Sample images
(Brightfield) (Fluorescence)
The OCC illuminator can be controlled using a slide lever.
Thanks to scales on the slide lever, the user can save and
reproduce desired illumination levels. In addition, an OCC
plate can be inserted into the illumination unit from the front
and rear sides, so images with different shadow direction
can be observed.
OCC stands for oblique coherent contrast, a form of oblique lighting method
developed by Nikon. Compared to conventional diascopic illumination that
illuminates directly from below, OCC illumination applies coherent light to
samples in a diagonal direction, adding contrast to colorless and transparent
sample structures.

Fertilized mouse egg 2 days old Transgenic Zebrafish embryo,

Image courtesy of Kazuo Yamagata, Ph.D., Center for
Genetic Analysis of Biological Responses, Research Institute
for Microbial Diseases, Osaka University
User-friendly remote control SMZ25
Board
Tg (isl1-GFP)
(using SHR Plan Apo 1x at zoom magnification of 6x with SMZ25)
Image courtesy of Hisaya Kakinuma, Ph.D., Laboratory for The all-new remote control provides easy access to zoom and focus controls and is designed
Developmental Gene Regulation, Developmental Brain Science for both right- and left-hand use. The remote control contains an LCD monitor with an
Group, RIKEN Brain Science Institute
adjustable backlight that provides at-a-glance information about zoom factor, objective lens,
filter cube and LED DIA brightness. The brightness of the LCD monitor and
LED indicators is adjustable

Automation and digital imaging SMZ25 SMZ18

A wide range of digital imaging capabilities with the Digital Sight series
and NIS-Elements imaging software LCD monitor with adjustable backlight provides
observation information.

Easily obtain the information required, such as Z drive position, zoom factor,
objective lens, filter cube and LED DIA brightness, by using the Digital Sight series
and NIS-Elements or Digital Sight series DS-L3 together with the microscope.

Detected observation condition/available control ◎：Detection and control of observation condition possible　◯：Detection of observation condition possible

SMZ25 SMZ18 On-axis imaging for digital images SMZ25 SMZ18

• Motorized focus unit • Manual focus unit

• Motorized epi-fluorescence set (control box A) • Manual epi-fluorescence set (relay box and control box B)
Easily switch between stereo position (stereoscopic view) and
DS-L3 NIS-Elements DS-L3 NIS-Elements mono position (on-axis view) when using the P2-RNI2 Intelligent Comparison of on-axis image and stereoscopic image
Zoom magnification ◯ ◎ ◯ ◯ Nosepiece by simply moving the objective lens.
Focusing ◯ ◎ ー ー

Objective (with nosepiece) ◯ ◯ ◯ ◯

Diascopic LED illumination stand

◯ ◎ ◯ ◎
(ON/OFF, light intensity control)

Fluorescence illuminator (light intensity control) ◯ ◎ ◯ ◎

Filter cube ◯ ◎ ◯ ◯ On-axis image Stereoscopic image

For other combinations, please confirm with Nikon.

* With NIS-Elements F (Free package), functions above are not available. Use NIS-Elemsnts D/Br/Ar.
6 7
Parallel-optics type
Stereo Microscope

Highest-in-class zoom ratio

Wide zoom range

The SMZ1270/1270i offers the highest-
in-class zoom ratio of 12.7x (0.63 – 8x).
It offers both low-magnification wide
viewfield observation of the whole of a
35 mm petri dish* during screening and
high-magnification observation of minute
cell structures
* with 1x objective at the lowest magnification.
The SMZ800N comes with a 1 – 8x zoom range, with higher
magnification than conventional models and enables high-
resolution observation of 640LP/mm (using ED Plan Apo 2x/WF at
maximum zoom).
Improved resolution of SMZ800N
SMZ1270/1270i enables
observation of the whole of a
35 mm petri dish.

Wide viewfield of SMZ1270/1270i

Ampulex

Incredible sharpness throughout a wide magnification range

0.63x zoom SMZ800N Conventional model
These versatile stereo microscopes provide both excellent optical performance, such as high-magnification, high-zoom 8x zoom

ratio and high-resolution images, and advanced operability. The expandability of parallel optics makes these models
suitable for a wide range of applications.
Newly developed objectives
Highest-in-class zoom ratio Easy to get results
The newly developed WF series objectives offer uniformly bright
• Highest-in-class zoom ratio of 12.7:1 (0.63 – 8x) with • Automatically detects magnification data in combination with images even at low magnification and wide viewfield observation
SMZ1270/1270i the digital camera control unit (SMZ1270i only) with SMZ1270/1270i. In addition, a 0.75x objective is now
•New WF series objectives optimized for wide viewfield • Nosepiece offers both widened magnification range and on- available, expanding the lineup of low magnification objectives.
observation at low magnification axis imaging
• Eyepiece tubes with various inclination angles and slim-type
stands minimize user fatigue during observation

High-quality images Expandable with a wide range of accessories

• High-level chromatic aberration correction provides sharp • A wide range of accessories are available, including eyepiece
images throughout the viewfield. tubes and stands that are equal to superior specification
stereo microscope models

High-quality images

Apochromat optics have been adopted for

the lenses in the SMZ1270/1270i zoom body
and semi-apochromat optics in the SMZ800N
to achieve high-level chromatic aberration
correction. They provide sharp images without
blur or color fringe.

Versatile stereo microscope with the The same as the SMZ1270 but equipped with Affordable model with improved
Apochromat optics Conventional optics
highest-in-class zoom ratio intelligent functions found in superior models operability and basic performance (captured with SMZ1270+Plan Apo 1x/WF)
8 (SMZ1270i with a trinocular tilting tube and nosepiece) 9
 Easy to get results Expandable with a wide range of accessories

Intelligent function for status readout SMZ1270i In addition to conventional

accessories, the level of accessories
In combination with the Camera Control Unit DS-L3 and imaging software NIS-Elements, the SMZ1270i can detect zoom magnification used with superior models is also
data. In addition, with the Intelligent Nosepiece P-RNI2 attached, data related to the objective in use is also detected. Calibration data is available for the SMZ1270/1270i and
automatically altered, following changes of magnification, to display the appropriate scale and measurement results on the images. SMZ800N. These include trinocular
tubes and slim-type LED diascopic
illumination stands. These allow
various microscope configurations Fibers Semiconductor Brain of adult drosophila excited with GFP
to suit numerous routine inspections Image courtesy of Hokto Kazama, Ph.D., Circuit
Mechanisms of Sensory Perception, Brain
and a range of research and Science Institute, RIKEN
DS series camera
development applications.

With the LED Diascopic

Stand-alone Control Unit DS-L3

OCC illumination Diascopic brightfield illumination Illumination Stand and
Fiber Diascopic Illumination
Stand, image contrast
under OCC illumination can
be easily adjusted.
• Detection of zoom
magnification and objective
information
•Calibration auto change

SMZ1270i + Intelligent Nosepiece

Imaging Software NIS-Elements OCC illumination boosts the contrast of transparent sample structures.
(with Microscope Camera DS-Ri2 / Hemicentrotus pulcherrimus in two-cell stage
PC-use Control Unit DS-U3)
Correct scale is always indicated

On-axis observation with the nosepiece SMZ1270 SMZ1270i SMZ800N

Optical system Parallel-optics type (zooming type)

Zoom ratio 12.7 : 1 8:1
The double nosepiece offers easy on-
axis imaging, enabling observation Without nosepiece On-axis imaging Zoom range 0.63 – 8x (0.63/1/2/3/4/6/8x stops) 1 – 8x (1/2/3/4/6/8x stops)
3.15 – 480x (depending on eyepiece and objectives) 5 – 480x (depending on eyepiece and objectives)
of the bottom of holes, accurate Total magnification
(with coaxial episcopic illuminator: 15 – 540x) (coaxial episcopic illuminator: 22.5 – 540x)
simple measurement and extended Tubes Eyepiece inclination: 20° (P-B Binocular Tube) / 15° (P-TL100 Trinocular Tube) / 0°-30° (P-TERG100 Trinocular Tilting Tube, P-TERG50 Trinocular Tilting Tube)
depth of focus (EDF) imaging without Eyepieces C-W10xB (F.N. 22), C-W15x (F.N. 16), C-W20x (F.N. 12.5), C-W30x (F.N. 7)
distortion. Plan Apo 0.5x/WF, Plan Apo 0.75x/WF, Plan Apo 1x/WF,
Objectives Plan Apo 0.5x/WF, Plan Apo 0.75x/WF, Plan Apo 1x/WF, ED Plan 1.5x/WF, ED Plan 2x/WF
ED Plan 1.5x/WF, ED Plan 2x/WF, Plan 1x, ED Plan 0.75x, Achro 0.5x

Working distance 70 mm (with Plan Apo 1x/WF) 78mm (with Plan 1x)
9.8 kg (with P-B Binocular Tube + P-DSL32 11.9 kg (with P-TERG100 Trinocular Tilting Tube +
Weight (approx.) 6.8 kg (with P-B Binocular Tube + C-PSN Plain Stand)
LED Diascopic Illumination Stand) P-DSL32 LED Diascopic Illumination Stand)
Please refer to the system diagram (P. 26-27) for accessory combinations.
On-axis view

Ergonomic design Dimensions

SMZ1270 set SMZ1270i set SMZ800N set

Eyepiece tubes with a range of
Observation posture SMZ1270 + P-B Binocular Tube + SMZ1270i + P-TERG100 Trinocular Tilting Tube + SMZ800N + P-B Binocular Tube +
inclination angles are available Plan Apo 1x/WF + P-PS32 Plain Stand Plan Apo 1x/WF + Intelligent Nosepiece P-RNI2 + C-PSN Plain Stand

for comfortable observation. SMZ1000 P-DSL32 LED Diascopic Illumination Stand

160 173 160
They offer the optimum eyelevel 140 Eyepoint level
(interpupillary
199 160 Eyepoint level
(interpupillary distance:
140 Eyepoint level
(interpupillary
199 130

200mm distance: 64 mm) distance:

to suit each user. In addition, 64 mm) 64 mm)

20°
slim-type plain stands and the Inclination
LED Diascopic Illumination angle 20°

146.5

133.5
Stand easily facilitate the 434mm

217.5

411
434

407

326
presentation and removal of

338
338

52
62

specimens. 244 Ø 60
160 Ø 60

136
78
70

70
244

27
35

35
With the LED Diascopic Illumination Stand and Fiber Diascopic 292 305
300 337 300 337
Illumination Stand, focus control during observation is possible
using the dial in front of the base.
10 11
Greenough type
Greenough Type Stereo Microscope Greenough Type Stereo Microscope

Designed for excellent cost

performance
• The SMZ445 has a 45° eyepiece tube inclination, and the SMZ460 has a 60° eyepiece tube
Superior 7.5x zoom and 115 mm working distance inclination, which is ideal for embedded use.
SMZ460 with C-FMBN
Focusing Mount BN
Trinocular optical head type is also available • Compact design with ease-of-use and high optical performance.
• ESD protection guards against electrostatic damage to samples.
SMZ445 with
• The SMZ745/745T boasts a 7.5x zoom that incorporates the Greenough C-PSN Plain Stand/CN
Specifications
optical system. The zoom range of 0.67x to 5x provides a broad
SMZ445 SMZ460
observation range.
Optical system Greenough type (zooming type)
• As well as high zoom ratio and magnification, the SMZ745/745T offers an Zooming ratio 4.4 : 1 4.3 : 1
unrivaled 115 mm working distance. Zooming range 0.8–3.5x 0.7–3x
• The SMZ745T incorporates an optical path switching lever that enables SMZ745 Total magnification 4-70× 3.5-60×
easy switchover between eyepiece and camera. A DS series digital camera Tube
Eyepiece inclination: 45° Interpupillary distance adjustment: 54–75 mm Eyepiece inclination: 60° Interpupillary distance adjustment: 54–75 mm
SMZ745T Eyepiece diopter adjustable for both eyes Eyepiece diopter adjustable for both eyes
can be attached. (with a DS series digital camera) Eyepieces SM 10xB (F.N. 21), SM 15xB (F.N. 14), SM 20xB (F.N. 12)
Auxiliary objectives (option) AL5 (0.5x), AL7 (0.7x)
Working distance 100 mm (standard)

Three “A” design (V) Weight (approx.) 1.0 kg (body) 1.1 kg (body)
2000
• Air-tight SMZ745
By making joints airtight, contamination from dust, oil, water and other Dimensions
contaminants is prevented.
1000 SMZ445 SMZ445 + C-PSCN Compact Stand/CN SMZ460 SMZ460 + C-FMBN Focusing Mount BN
Airtight construction: JIS Degrees of protection provided by enclosures IPX1
154 131 185 154 148
• Anti-mold SMZ745 SMZ745T 46 90 46

Anti-mold design developed exclusively by Nikon ensures peace of mind when E.P

the microscope is used in environments subject to high heat or humidity. 0 1 2

(sec)
• Anti-electrostaic SMZ745 SMZ745T
Static electricity built up within the microscope is discharged almost instantly, ensuring higher yields.

326
Antistatic function: 1000–10V, discharge within 0.2 sec.

325.5

330

25

53
73

26
φ52

101
159

100

35
φ52
φ76 φ15.8

100
26.5
Specifications
180 265 Unit: mm
SMZ745 SMZ745T

Optical system Greenough type (zooming type) Greenough type (zooming type), trinocular tube
Zoom ratio 7.5 : 1
Zoom range 0.67-5x (with 0.67/1/2/3/4/5x stops)
Greenough Type Stereo Microscope
Total magnification 3.35-300x (depending on eyepiece and auxiliary objective used)

Straight tube ー Built-in C-mount 0.55x magnification lens (F.N. 11), compatible with 2/3 in. or smaller CCD
Tubes Fixed type Eyepiece inclination: 45 ° Interpupillary distance adjustment: 52-75 mm

Eyepieces (with diopter adjustment) C-W 10xB (F.N. 22), C-W 15x (F.N. 16), C-W 20x (F.N. 12.5), C-W 30x (F.N. 7)

Auxiliary objectives G-AL 0.5x (W.D. 211 mm), G-AL 0.7x (W.D. 150 mm), G-AL 1.5x (W.D. 61 mm), G-AL 2x (W.D. 43.5 mm), G-AL ERG 0.77–1.06x (W.D. 102–48mm)
High-resolution optics ideal for inspection, assembly, and measurement
Working distance 115 mm (standard)
Airtight construction JIS Degrees of protection provided by enclosures IPX1 ー
• Compact design with horizontally positioned zooming ring (rotation: 90º)
SMZ-2 (Clemmer is optional)
Weight (approx.) 1.6 kg (body) 1.8 kg (body)
• Eyepiece inclination of 45° for comfortable observation
F.N.: Field Number

Dimensions Specifications Dimensions

SMZ-2
SMZ745 set SMZ745 + C-PSN Plain Stand/CN SMZ745T set SMZ745T + C-PSN Plain Stand/CN 120
Optical system Greenough type (zooming type)
164 150 164 140 45
Zooming ratio 5:1
Zooming range 0.8–4x
45°

Total magnification 4–120x (Depending on eyepiece and auxiliary objective used.)

Tube Eyepiece inclination: 45° Interpupillary distance adjustment: 56–75 mm

280
Eyepieces (with SM E10xA (F.N. 23, standard), SM E15xA (F.N. 14), SM 20xB (F.N. 12),
389

diopter adjustment) C-W30x (F.N. 7)

385

325

325

Auxiliary objectives AL5 (0.5x), AL7 (0.7x)

Ø60
Ø60 Working distance 77.5 mm (with standard configuration)
W.D.: 115
26 W.D.: 115

77.5
159 159
Weight (approx.) 1.6 kg (body), 1.9 kg (stand)
135

24.3
190×144
26

292 305 292 305 Unit: mm Unit: mm

12 13
Accessories SMZ25 SMZ18

Wide range of dedicated accessories for SMZ25/SMZ18 for SHR Plan Apo Objective Series

all types of observation The SHR Plan Apo series features higher NA, wider field of view and superior flatness and color aberration correction.
These objective lenses can be seamlessly switched because all magnifications have the same parfocal distance. The new bayonet mount
design allows lenses to be safely and easily removed.
SHR Plan SHR Plan SHR Plan SHR Plan
❸ ❹ Apo 0.5× Apo 1× Apo 1.6× Apo 2×
Base Unit, Focus Unit, Stand/Focus Mount
❷ SMZ25 0.078 0.156 0.25 0.321
❶ Maximum
NA
SMZ18 0.075 0.15 0.24 0.3
Base Unit
Working distance 71 mm 60 mm 30 mm 20 mm
Nikon has improved ease of use by moving the controls to the front of the base, including the brightness adjustment dial and the on/off switch. 3 mm water
Correction ring — — —
depth
Fiber DIA base Slim Bases Wavelength 380-700 nm
The Fiber DIA base features condenser lenses that can be switched The slimmer LED DIA Base and Plain Base help increase efficiency
❶ P2-SHR Plan Apo 0.5x　　　❷ P2-SHR Plan Apo 1x
between low and high magnifications. Furthermore, the OCC of sample manipulation by bringing the level of the sample closer to ❸ P2-SHR Plan Apo 1.6x　　　❹ P2-SHR Plan Apo 2x
illumination system allows high-contrast illumination. the table.

❶ Tubes

Conventional base
Choose from two types of tilting trinocular tube and one type of low
eyelevel trinocular tube. All tubes have a camera port for seamless
❷ P2-DBL LED Diascopic integration with the Digital Sight series. ❸
Illumination Base

❷
❸
❶ P2-DBF Fiber Diascopic Illumination Base ❶
Slim base
❸ P2-TL100 Trinocular Tube L (eyepiece: port 100:0 / 0:100)

❷ P2-TERG50 Trinocular Tilting Tube (eyepiece: port 100:0/50:50)

❸ P2-PB Plain Base
❶ P2-TERG100 Trinocular Tilting Tube (eyepiece: port 100:0 / 0:100)

Focus Unit Stand/Focus Mount SMZ18

The focus unit is combined with the base unit. Choose from either SMZ18 can be mounted on various compact stands using a focus Nosepiece/Focus Mount Adapter Stage
a manual or motorized focus unit. mount.
Both single and double nosepieces are available. The stage features an XY stroke of 6x4* inches (150 mm x
100 mm) and can be attached to any of the bases, making it
❷ effective for capturing large images when used in combination with
❶ imaging software NIS-Elements. A sliding stage and tilting stage are
❶
also available. *Limited Y travel with 32 mm column bases
❹
❷
❷

❹ P-DSF32 Fiber Diascopic

❶ Illumination Stand ❷ P2-FM Focus Mount Adapter
❶ P2-FMDN Focus Mount
❷ P-PS32 Plain Stand

❷ P2-FU Focus Unit

❸
❶ P2-RNI2 Intelligent Nosepiece

❶ P2-MFU Motorized Focus Unit ❸ P-DSL32 LED Diascopic Illumination Stand

P-SXY XY Stage

14 15
Accessories SMZ25 SMZ18

Remote Control Fiber Illuminator Set

Nikon offers a remote control unit that can be used to Flexible Double Arm Fiber Illumination Set Ring Fiber Illumination Set
operate the microscope and capture images by hand. A The direction and angle of illumination can be changed to suit the This ring fiber illumination set features an episcopic illumination
footswitch is also available, allowing the user to operate the ❶ ❷ sample by making adjustments with these double arms. The fiber unit that effectively captures images (can be used with 1x and 0.5x
microscope and capture images by foot, freeing the hands holder position can also be changed to obtain the optimal position objective lenses).
for sample manipulation. for illuminating samples. ❶ P2-FIR Ring Fiber Illumination Unit
❶ C-FDF Flexible Double Arm Fiber Illumination Unit ❷ C-FLED2 LED Light Source for Fiber Illuminator
❷ C-FIDH Fiber Holder
❸ C-FLED2 LED Light Source for Fiber Illuminator
❶ AZ-PCR Photo Release
❷ AZ-FSW Foot Switch
P2-RC Remote Controller

Darkfield Observation Accessory Polarizing Observation Accessory

Darkfield viewing is
possible simply by
attaching the darkfield unit
to the base.
❶ P-DF LED Dark Field Unit
❷ Shading cover
The analyzer is attached ❶ ❷
to the objective and the
❷ polarizer to the base or
stand to enable polarized ❶
❶ viewing. ❸
❶P
 2-POL Simple Polarizing ❶ ❷
Attachment

Combinations with SMZ18 Combinations with SMZ18

Epi-fluorescence Set
Coaxial Illuminator Ring LED Illuminator
Motorized Epi-fluorescence Set
The fluorescent turret can be operated using the ❼
❷ ❹ The coaxial light illuminator makes it possible to view light reflected Ring LED illuminator is equipped with high-intensity, long-life (20,000
remote control or imaging software NIS-Elements. from the surface of a sample. It is ideal for shooting shadow-less hours) LEDs. The illuminator’s dial adjusts the intensity of the white
❶ images of thick samples. LED.
❶ P2-EFLM Motorized Epi Fluorescence Attachment
❶ P2-CI Coaxial Epi Illuminator ❶ P2-FIRL LED Ring Illumination Unit
❷ Light shading Plate (comes with Fluorescence Attachment) ❺ ❷ C-FLED2 LED Light Source for Fiber Illuminator
❸ P2-EFL Filter Cube (GFP-B/GFP-L/RFP) ❸ ❸ P2-CIA QL1x/0.5x 1/4λ Plate
❹ P2-EFLBF Filter Cube (Bright Field)
❻
❺ P2-CTLA Control Box
❻ P2-RC Remote Controller
❼ P2-CIA QL1x/0.5x 1/4λ Plate

Combinations with SMZ25

Manual Epi-fluorescence Set

An easy-to-use manual model for Nikon’s newly ❹ ❻ ❶
developed high-performance epi-fluorescence ❷ ❷
attachment. ❶ ❶
❸
❶ P2-EFLI Epi Fluorescence Attachment ❸
❷ Light shading Plate (comes with Fluorescence Attachment) ❺
❸ P2-EFL Filter Cube (GFP-B/GFP-L/RFP)
❹ P2-EFLBF Filter Cube (Bright Field)
❺ P2-CTLB Control Box Combinations with SMZ18 Combinations with SMZ25
❻ P2-CIA QL1x/0.5x 1/4λ Plate

Combinations with SMZ18

16 17
Accessories
Nosepieces
Double nosepiece with two-objective switchover. Easy changeover P-RN2 Nosepiece SMZ1270/1270i SMZ800N
from stereo position (stereoscopic view) to mono position (on-axis
view) is possible by simply moving the objective lens to the right. Observations with wider zoom
ranges are possible by simply
A variety of accessories are available switching between two objectives.

for stereoscopic observations

P-RNI2 Intelligent Nosepiece SMZ1270i

Enables easy switchover between

two objectives. In combination
with the Digital Sight series digital
Plan Apo WF series camera, it automatically detects
the data of objective in use.
Objectives
立体視 view
Stereoscopic 垂直視
On-axis view

A wide selection with various magnifications and working distances is available, including high-NA, high-resolution and wide-viewfield Plan
Apo WF series objectives with superior image flatness and chromatic aberration correction.

SMZ1270/1270i SMZ800N Working Zoom Actual

Tubes/Eye-level Riser SMZ1270/1270i SMZ800N
Objectives NA
distance (mm) magnification FOV*1
Various ergonomic tubes with different inclination angles enable suitable eye levels to be selected for observation, even when an intermediate
0.63x 0.0095 69.8
0.5x/WF 82 tube or illuminator is attached. Trinocular tubes are also equipped with camera ports.
8x 0.0525 5.5

Plan
0.75x/WF 107
0.63x 0.0143 46.6 P-B Binocular Tube P-TERG100/P-TERG50 Trinocular Tilting Tube
Apo 8x 0.0788 3.7
0.63x 0.0190 34.9 20° inclination angle allows Allows continuous adjustment of the
1x/WF 70
observation without having to lean eyepiece inclination from 0° to 30°.
❶ ❷ ❸ ❹ ❺
8x 0.1050 2.8
forward and reduces fatigue during Optical path switching ratio of
0.63x 0.0285 23.3
❶ Plan Apo 0.5x/WF ❹ ED Plan 1.5x/WF
1.5x/WF 44 long-time operations. eyepiece:camera port is 100:0/0:100
ED 8x 0.1575 1.8
❷ Plan Apo 0.75x/WF ❺ ED Plan 2x/WF Plan with P-TERG100 and 100:0/50:50 with
0.63x 0.0380 17.5
❸ Plan Apo 1x/WF 2x/WF 35 P-TERG50. P-TERG100 Trinocular Tilting Tube
8x 0.2100 1.4

*1 With C-W10xB eyepiece

P-TL100 Trinocular Tube P-IER Eye-level Riser
SMZ800N Working Zoom Actual
Objectives NA
distance (mm) magnification FOV*1 15° of inclination angle allows observation Increases the eyepoint height 25 mm per riser for
1x 0.0145 44 with comfortable posture even when using a total of 50 mm.
Achro 0.5x 189
❶ Achro 0.5x 8x 0.0525 5.5 a thick stand or intermediate tube.
❷ ED Plan 0.75x Optical path switching ratio of
1x 0.0218 29.3
❸ Plan 1x ED Plan 0.75x 117 eyepiece:camera port is 100:0/0:100.
8x 0.0788 3.7
❶ ❷ ❸
1x 0.0290 22
Plan 1x 78
8x 0.1050 2.75

*1 With C-W10xB eyepiece

Intermediate Tubes SMZ1270/1270i SMZ800N

Auxiliary Objectives Various intermediate accessories are available that can be inserted between the microscope zooming body and tube.

P-IBSS2 Beam Splitter S2 P-THSS Teaching Head P-IDT Drawing Tube

Microscopes Auxiliary objectives Working distance (mm) Microscopes Auxiliary objectives Working distance (mm)

G-AL ERG 0.77–1.06x 102–48 AL5 (0.5x) 181

SMZ445/460 Using a beam splitter and camera adapter, Simultaneous observation of the same Drawing sample images is possible by
G-AL 0.5x 211 AL7 (0.7x) 127.5
a digital camera can be attached to the viewfield is possible between the eyepiece simply tracing observed images that are
SMZ745/745T G-AL 0.7x 150 AL5 (0.5x) 103 binocular eyepiece tube for imaging. lenses of both teaching head and overlaid on top of drawings within the
SMZ-2
G-AL 1.5x 61 AL7 (0.7x) 95 Optical path switching ratio of left microscope, making it ideal for educational viewfield. The drawings can be removed
G-AL 2x 43.5 eyepiece:right eyepiece:camera port is purposes. The pointer can indicate target from view by using the knob to block the
100:100:0/100:50:50. points in the viewfield during observation. light path.
18 19
Accessories
Stages Illumination Systems
Stages allow smooth sample movement in order to change viewfield during observation. Provides a cone of light from above the sample to the center, minimizing unwanted shadow. Suitable for
Ring Illuminator
observation of electronic substrates.
C-SSL Dia-sliding Stage Circular Floating Stage 2

Used for diascopic observation, Used for episcopic observation.

this sliding stage can be easily Loaded with a sample, the stage
moved in the desired direction can be easily moved in the desired
simply with a light push. Travel direction simply with a light push
range is within ø38mm. to its edges. Travel range is within
SMZ1270/1270i SMZ800N ø40mm. C-FIR Plastic Fiber-optics Ring LMS100 x 60-15W LED Ring Light SM-LW61Ji3 LED Ring Illuminator
SMZ745/745T SMZ445/460 SMZ1270/1270i SMZ800N Illuminator
Can be used with the SMZ25 and SMZ18 SMZ745/745T SMZ-2
SMZ445/460
Illuminator is located away from microscope. Color temperature is adjusted to 6500K Three types of covers are available (clear,
Can be used with the SMZ25 and SMZ18
It enables bright observation with high- ± 500K to provide stable illumination. diffuser and opaque white). Anti-electrostatic
intensity light without damaging sample with Two types of covers are available. Anti- type
C-TRS Tilting Stage P-SXY XY Stage its heat. electrostatic type SMZ1270/1270i SMZ800N SMZ745/745T
SMZ1270/1270i SMZ800N SMZ745/745T SMZ1270/1270i SMZ800N SMZ745/745T SMZ445/460 ※ SMZ-2 ※

SMZ445/460 ※ SMZ-2 ※ SMZ445/460 ※ SMZ-2 ※

This stage has a nonslip sheet The stage features an XY stroke
and can be tilted 30° from its of 150 mm x 65 mm. By attaching * G-OBA60 Adapter is required.

horizontal position. AZ100 stage adapters, it

SMZ1270/1270i SMZ800N can be used for various Arm Illuminator/Episcopic The direction and angle of the illumination can be changed with simple adjustments of the
SMZ745/745T SMZ445/460 applications. It can be Illuminator flexible arm.
SMZ-2 used with both diascopic
Can be used with the SMZ25 and SMZ18 and episcopic illuminators. Arms for LED Episcopic Illuminator

SMZ1270/1270i SMZ800N
SMZ745/745T SMZ445/460

Observation Attachments
Various observation accessories are available that utilize diascopic and episcopic illuminations. They can be used for samples that are difficult SMZ-U Episcopic Arm G-EIA Flexible Arm
to observe using standard illumination.
C-FID2 Double Arm Fiber C-FDF Flexible Double Arm C-LSL LED Episcopic Illuminator
P-EFL Epi-fluorescence Attachment P-DF LED Darkfield Unit Illuminator Fiber Illumination Unit

Up to four epi-fluorescence filter cubes can be mounted. The fly- Equipped with the while light LED as the light source. Simply It enables bright observation with It enables bright observation with In combination with C-PSN Plain Stand/CN and C-PSCN
eye lens provides bright illumination up to the viewfield periphery. placing the unit on the stage enables darkfield observation. high-intensity light without damaging high-intensity light without damaging Compact Stand/CN, illumination angle flexibility is possible
SMZ1270/1270i SMZ1270/1270i sample with its heat. The direction sample with its heat. The direction from the back of the microscope.
SMZ800N SMZ800N
and angle of illumination can be and angle of illumination can be By attaching arms, flexible change of direction and angle of
SMZ745/745T
changed using the flexible arms. changed using the fiber holder. illumination is possible.
SMZ1270/1270i SMZ800N SMZ1270/1270i SMZ800N SMZ1270/1270i SMZ800N SMZ745/745T SMZ445/460
SMZ745/745T SMZ445/460 SMZ745/745T SMZ445/460
SMZ-2

Suitable for brightfield observation for high-reflectance flat surface samples such as polished metals
Coaxial Illuminator
and wafers.
C-POL Polarizing Attachment
P-CI Coaxial Episcopic Illuminator G-ICIL LED Coaxial Episcopic Illuminator
Simple polarizing observation
Coaxial illuminator for parallel optics-type Coaxial illuminator for Greenough-type stereo
is possible by placing the
stereo microscopes. Provides high-intensity microscopes. Equipped with both coaxial
polarizer on the stage while the
illumination for the entire view field. episcopic and oblique illumination, which
analyzer is attached to the tip
*1/4λ plate is required illuminates from behind the microscope.
of the objective lens. SMZ1270/1270i SMZ800N SMZ745/745T
SMZ1270/1270i SMZ800N
SMZ745/745T

20 21
Accessories
Stands Universal Table Stands/Focusing Mounts

Universal Table Stands G-US1/G-US2 Universal Table Stand P

These stands are handy in microscopy with large samples not Not only can it be used for a large sample, but this extremely stable
loaded onto the standard stand. The microscope zooming body is stand also easily accommodates intermediate tubes.
mounted to the stand arm via a focusing mount. The G-US1 is a - Used in conjunction with the C-FMAN Focusing Mount AN on the SMZ1270/
table clamp type (table top thickness: 10 to 60 mm). 1270i/800N/745/745T/445/460.
- Used in conjunction with the C-FMBN Focusing Mount BN on the - Used in conjunction with the SM Focusing Mount on the SMZ-2.
SMZ1270/1270i/800N/SMZ745/745T/445/460.
- Used in conjunction with the SM Focusing Mount and the G-USA SM US Universal Stand P
C-PSN
C-PSCN
Adapter on the SMZ-2.
C-PSN Plain Stand/CN, C-PSCN P-PS32 Plain Stand C-LEDS Hybrid LED Stand - Cannot be used with the SMZ1270/1270i/800N when intermediate tube is
mounted on these models.
Compact Stand/CN
G-US1 Universal Table Stand 1
Offers a comfortable work area and Features a slim design with a ø180 Both episcopic and diascopic 413
allows easy handling of samples. mm stage plate and 160 mm width observations are possible and can
C-PSCN has a small base that saves between the pillar and optical axis to be conducted simultaneously. The

φ28
desk space. boost working efficiency. space-saving built-in illuminator can be

452
φ38
switched and adjusted with ease.

25
364
138-410 315
Type Episcopic Episcopic Episcopic/Diascopic 90-350 148 Can be installed on the top
or bottom edge.
Illumination φ28
method － － Epi-oblique*, brightfield

The image is a configuration sample with the SMZ745.

Built-in filter － － － 10-60
Unit: mm

126
Fine focus
knob － － － Specifications
Universal Table Stand
Observation
magnification With all objectives, at all zoom ranges With all objectives, at all zoom ranges With all objectives, at all zoom ranges
G-US2 Universal Table Stand 2 Model G-US1 G-US2 P

SMZ1270/1270i SMZ800N SMZ745/745T SMZ445/460 Vertical cross travel 245mm 229mm

Microscopes 413
P-PS32 can be used with the SMZ18. Horizontal cross travel 260mm 272mm
* The illumination area is limited by conditions of use. Weight (approx.) 4.4kg 23.0kg 30.5kg

φ28
C-FMAN Focusing Mount AN — ○

○ —

25
C-FMBN Focusing Mount BN

364
C-FMCN Focusing Mount CN — —

SM Focusing Mount ○* ○
○: Possible * G-USA Adapter is required
φ28
123-383 G-USA Adapter
35 φ24.5

75

φ15.8
The image is a configuration
230 sample with the SM
Focusing Mount.

117
95
Unit: mm The image is a configuration sample with the SMZ745.
C-DS Diascopic Stand S P-DSL32 LED Diascopic P-DSF32 Fiber Diascopic
Illumination Stand Illumination Stand
Focusing Mounts
Features a hand rest for comfortable The OCC illumination system allows Light source is located away
operation. Used in conjunction with colorless and transparent samples to be from microscope, enabling bright Various types of focusing mounts are available depending on use. They are used to incorporate stereo microscope bodies into IC bonders or
C-DSLU LED Unit for Dia Illumination observed in high relief. Compact slim- observation with high-intensity light other devices (SM Focusing Mount is for SMZ-2). These mounts can also be used when attaching microscopes to Universal Table Stands.
Stand. type base enhances operation efficiency. without damaging sample with its heat.
C-FMAN Focusing Mount AN C-FMBN Focusing Mount BN C-FMCN Focusing Mount CN
SM Focusing Mount
Type Diascopic Diascopic Diascopic 101 148 160

30 25

50
Illumination Brightfield Brightfield, OCC** Brightfield, OCC** 100
method 74

25

53
101
Not required φ24.5

35
φ76
Built-in filter － NCB11, ND4/16 φ32
(ø45 mm filter slot provided) ø76mm: C-FMAN Focusing Mount AN Ø15.8±0.03
ø62mm: SM Focusing Mount Unit: mm
Fine focus
knob － Included Included C-FMAN Focusing Mount AN C-FMBN Focusing Mount BN C-FMCN Focusing Mount CN SM Focusing Mount

Observation 0.5x objective is compatible with zoom 0.5x objective is compatible with zoom Focusing area 40mm 50mm 50mm 40mm
magnification With all objectives, at all zoom ranges
magnifications higher than 1.5x. magnifications higher than 1.5x.
Weight (approx.) 0.6kg 0.8kg 1.6kg 0.6kg
SMZ1270/1270i SMZ800N SMZ745/745T SMZ445/460
Microscopes Antistatic function 〇 〇 － －
P-DSL32 and P-DSF32 can be used with the SMZ18.　
Compatible microscopes SMZ1270/1270i/800N/745/745T/445/460 SMZ-2
** Conditions of use vary depending on objective in use.

22 23
System Diagrams (SMZ25/SMZ18) Specifications/Dimensions (SMZ25/SMZ18)
Specifications
DS-Ri2/Qi2
C-W10×B C-W15× C-W20× C-W30× 1/1.8” Camera 2/3” Camera C-mount CCD Camera SMZ25 SMZ18
Eyepiece Eyepiece Eyepiece Eyepiece
C-mount Adapter C-mount Adapter C-mount Zooming body
0.55× 0.7× TV Adapter A F-mount F-mount
Adapter Adapter
Optical system Parallel-optics type (zooming type), apochromatic optical system
2.5×
A LV-TV Zoom Motorized Manual
TV Adapter
Zoom ratio 25:1 18:1

AZ-PCR
Zoom range 0.63-15.75x 0.75-13.5x (with 0.75/1/2/3/4/5/6/8/10/12/13.5x click stops)
Photo Release
A A A
Aperture diaphragm Zooming body built-in
Objectives NA, WD (mm)
P2-TERG100 P2-TERG50 P2-TL100 P2-RC
Trinocular Tilting Tube Trinocular Tilting Tube Trinocular Tube L AZ-FSW
Remote P2-SHR Plan Apo 2x 0.312, 20 (with a correction ring for water 0 to 3 mm in depth) 0.3, 20 (with a correction ring for water 0 to 3 mm in depth)
Foot Switch
Controller
C-HGFI/C-HGFIE P2-SHR Plan Apo 1.6x 0.25, 30 0.24, 30
HG Precentered Fiber Illuminator
P2-EFLM P2-SHR Plan Apo 1x 0.156, 60 0.15, 60
Motorized Epi
Fluorescence Attachment P2-SHR Plan Apo 0.5x 0.078, 71 0.075, 71
X (with Light Shading Plate)
a P2-EFL Total Magnification
Filter Cube (GFP-B/GFP-L /RFP) 3.15-315x (depending on objective used) 3.75-270x (depending on objective used)
(using C-W10xB eyepieces)
P2-EFLI
P2-EFLC
Eyepieces (F.O.V. mm) • C-W10xB (22) • C-W 15x (16) • C-W 20x (12.5) • C-W 30x (7)
Epi Fluorescence
Filter Cube Y
Attachment • P2-TERG 100 Trinocular Tilting Tube (100/0 : 0/100)
X (with Light Shading Plate) • P2-TERG 50 Trinocular Tilting Tube (100/0 : 50/50) Inclination angle : 0-30 degree
P2-CIA QL a P2-EFLBF Tubes (eyepiece/port)
a 0.5x 1/4λ Plate Filter Cube • P2-TL100 Trinocular Tube L (100/0 : 0/100) Inclination angle : 15 degree
D (Bright Field) X P2-CTLA
P2-CI
Control Box Focusing Unit (stroke from • P2-MFU Motorized Focus Unit (up 96 mm/down 4 mm)
P2-CIA QL Coaxial Epi objective’s parfocal point) • P2-FU Focus Unit (up 97 mm/down 5 mm)
a
1x 1/4λ Plate Illuminator P2-MFU
• P2-FM Focus Mount Adapter
Motorized Focus Unit • P2-FM Focus Mount Adapter
Focus mount adapter/nosepiece • P2-RNI2 Intelligent Nosepiece (2 objectives can be attached)
P2-FM • P2-RNI2 Intelligent Nosepiece (2 objectives can be attached)
SU-AC AC Adapter • P2-FMDN Focus Mount (for P-PS32/P-DSL32/P-DSF32 stands)
Focus Mount Adapter
B • P2-PB Plain Base • P2-DBL LED Diascopic Illumination Base (OCC illuminator built-in) • P2-DBF Fiber Diascopic Illumination Base
Bases/stand • P-PS32 Plain Stand (only for SMZ18) • P-DSL32 LED Diascopic Illumination Stand (OCC illuminator built in) (only for SMZ18)
• P-DSF32 Fiber Diascopic Illumination Stand (only for SMZ18)
C Stages • P-SXY64 Stage • C-SSL DIA Sliding Stage • C-TRS Tilting Stage
X
X X P2-RLY
X P2-RNI2 Relay Box Bright Field, Epi Fluorescence, Simple Polarizing (with P2-POL Simple Polarizing Attachment), Dark Field (with P-DF LED Dark Field Unit),
SMZ18 SMZ25 Intelligent Nosepiece Y Observation methods
Zooming body Zooming body Oblique Lighting
4 filter cubes mountable, fly-eye lens built-in
P2-FU
Epi-fluorescence attachments
Focus Unit P2-CTLB • P2-EFLM Motorized Epi Fluorescence Attachment • P2-EFLI Epi Fluorescence Attachment
Control Box
Epi-fluorescence light sources • HG Precentered Fiber Illuminator Intensilight C-HGFIE HG/C-HGFI HG (130W)
B
C • P2-FIRL LED Ring Illumination Unit
P2-SHR Plan P2-SHR Plan P2-RLYC
Apo 0.5× Apo 1× P2-SHR Plan P2-SHR Plan Relay Cable
Episcopic illuminators Use with fiber light source
Objective Objective Apo 1.6× Apo 2× D • P2-CI Coaxial Epi Illuminator • P2-FIR Ring Fiber Illumination Unit • C-FDF Flexible Double Arm Fiber Illumination Unit
Objective Objective
Episcopic light source • C-FLED2 LED Light Source for fiber illuminator
32 kg 30 kg
(Motorized Epi Fluorescence Attachment configuration with (Epi Fluorescence Attachment configuration with Trinocular
P2-POL Weight (approx.)
P2-FIRL
Simple Polarizing
P2-FIR Trinocular Tilting Tube, Motorized Focus Unit, Intelligent Nosepiece, Tilting Tube, Focus Unit, Intelligent Nosepiece, LED DIA Base and
LED Ring Ring Fiber Illumination Unit
Illumination Attachment LED DIA Base and Objectives 1x and 0.5x) Objectives 1x and 0.5x)
Unit Z AZLED-PCU Power Code
J 30W
Z
10W
(Motorized Epi Fluorescence Attachment configuration with Trinocular
Power consumption (approx.) (Epi Fluorescence Attachment configuration with Trinocular Tilting
Power cable Tilting Tube, Motorized Focus Unit, Intelligent Nosepiece and LED
Tube, Focus Unit, Intelligent Nosepiece and LED DIA Base)
DIA Base)
P-DF AC Adapter 2
LED Dark Field Unit

Dimensions
C-FDF C-FLED2
Z Flexible Double Arm LED Light Source for Fiber Illuminator
Stage Adapters
for AZ Shading Fiber Illumination Unit
cover

C-SSL C-TRS (configured with motorized epi-fluorescence (configured with epi-fluorescence (configured with plain stand and
DIA Sliding Stage Tilting Stage attachment and LED DIA base) attachment and LED DIA base) focus mount)
C P2-FMDN
C-FIDH Focus Mount
Fiber Holder C-EP Extension Pillar
P-SXY64
XY Stage
H I G
B

298

298

300
H I J

H I H I 514 25 514 25 337 (44)

G 302
H I J 244
G G G
G
D

P2-PB P2-DBL LED Diascopic P2-DBF H I J

Plain Base Illumination Base Fiber Diascopic P-PS32
Illumination Base Plain Stand

569

569
G P-DSF32 Fiber Diascopic
Y AC Adapter 2

492
Illumination Stand

471

471

429
P-DSL32 LED Diascopic
Illumination Stand
AC Adapter 2

Power cable 444 444

24 25
System Diagrams (SMZ1270/1270i, SMZ800N, SMZ745/745T)

SMZ1270/1270i/800N C Illuminators
C-FID2 Double Arm Fiber Illuminator
A SMZ1270i
SMZ800N
Zooming Body Zooming Body
SMZ1270
Zooming Body
B B B C-LSL LED
Epi Illuminator
D D D
● ● ● ⑬ ④
P-TERG100 P-TERG50 P-TL100 P-B
Trinocular Trinocular Trinocular Tube L Binocular Tube G-EIA Flexible C-FDF Flexible Double Arm Fiber
P-RN2 Nosepiece Illumination Unit
Tilting Tube Tilting Tube P-RNI2 Supporting Arm
Intelligent Nosepiece SMZ-U
Articulated
Achro Arm AC Adapter 2
ED Plan Plan
0.5X 0.75X 1X
① ② ③ Power Cable ⑬
C-FLED2 LED
Plan Apo Plan Apo Plan Apo ED Plan ED Plan Light Source for
0.5X/WF 0.75X/WF Fiber Illuminator AZ-LED
1X/WF 1.5X/WF 2X/WF Power Codes
C-FIDH Fiber Holder
E E
Tubes with the ● symbol cannot be used in combination with stands with the ■ symbol (p. 27).
⑤

P-DF LED P-DF-LSP Light

C-POL Polarizing C-FIR Plastic Fiber-optics Dark Field Unit
SMZ745/745T Attachment
LMS100 x 60-15W
Ring Illuminator
⑫
Shielding Plate

LED Ring Light

A C-mount Camera F
SM-LW61Ji3 LED C-LP 1/4λ Plate
⑬
Ring Illuminator
⑩
G-ICIL LED Auxiliary objectives
SMZ745 SMZ745T Coaxial Episcopic
Zooming Body Zooming Body Illuminator
D D G-AL 0.29x G-AL 0.5x G-AL 0.7x G-AL 1.5x G-AL 2x
Stands/Stages D

Power cable C-ER Extension Ring Adapter

E for Achro 0.5x Objective

①
① ②
② ③
③
Eyepieces/Camera Adapters C-mount Camera
⑦⑩⑫
Rubber Rubber Horn-shaped ⑥⑧⑨ ④ ⑧
Eyeguard Eyeguard 25 Rubber Eyeguard Eyepieces C-0.55x DS C-0.7x Relay Lens C-mount
Relay Lens for DXM TV Adapter A
C-W10xB C-W15x C-W20x C-W30x ■ ■ ■
C-PSN Plain Stand/CN, C-LEDS Hybrid LED Stand C-DSLU LED Unit for
C-DS Diascopic
LV-TV Tube Adapter C-PSCN Compact Stand/CN Stand S ⑬ Dia Illumination Stand
Power Cable
①
C-FC Reticle C-FM Reticle A B ① ②
(Crosshairs) (Micrometer) C-FMAN Focusing
②
Mount AN ③ C-FMCN
Focusing Mount CN
C-FMBN
C-EP Extension Pillar
Focusing Mount BN (SMZ1270/1270i/800N: used with
auxiliary objectives 0.5x, 0.7x
SMZ745/745T: used with auxiliary
Intermediate Tubes B
objectives 0.5x, 0.7x)
HG Precentered Fiber Illuminator "Intensilight"

P-IER Eye-level Riser Filter cubes

P-IBSS2 P-EFL GFP-B

① ①
Beam Splitter S2 GFP-L Filter Cube ① ① ④
② ⑦⑨⑩⑪⑫ ④ ⑦⑩⑪⑫ ④
P-EFLC Filter Cube ⑦⑩⑪⑫ ⑤
P-EFL Epi ⑤ ⑤
P-EFLBF Filter Cube (Bright Field) F
Fluorescense ●
Attachment
P-CL QL1X 1/4λ Plate P-CL QL1.5X 1/4λ Plate G-US1 Universal G-US2 Universal
Universal Table Stand P Table Stand 1 Table Stand 2 P-PS32 Plain Stand P-DSL32 LED Diascopic
P-DSF32 Fiber Diascopic
P-THSS Teaching Head Illumination Stand
Illumination Stand
AC Adapter 2

P-IDT Drawing Tube
P-CI Coaxial Epi Illuminator
Iris Diaphragm
C P-CL QL1X 1/4λ Plate
Power Cable
C-ASP180 Opal & C-GSP180 Glass Stage Adapters for AZ
Y-THPL LED Pointer Unit for Teaching Head SM Opal & Black SMZ Black Acrylic Plate Plate 180 mm ⑥ ⑦
Acrylic Plate Glass Plate ESD Plate
F TH-AC2 AC Adapter for TH Pointer Circular Floating
Stage 2*
Power Cable
P-THS Teaching Head Stand
⑧ ⑦ P-SXY
C-TRS Tilting * Cannot be used C-SSL DIA XY Stage
Stage
⑧ ⑨ observation.
for diascopic
Stands with the ■ symbol cannot be used in combination with tubes and Sliding Stage
Epi fluorescense attachments with the ● symbol cannot be used in ⑩ ⑪
P-CL QL1.5X 1/4λ Plate
combination with stands with the ■ symbol (p. 27). epi fluorescense attachments with the ● symbol (p. 26).

26 27
Specifications

Parallel-optics type

Model SMZ25 SMZ18 SMZ1270 SMZ1270i SMZ800N

Optical system Parallel-optics type (zooming type) Parallel-optics type (zooming type)

Zoom ratio 25:1 18:1 12.7:1 8:1

Zoom range 0.63-15.75x 0.75-13.5x 0.63 – 8x 1 – 8x

Total magnification* (When coaxial 3.15 – 480x (depending on eyepiece and objectives) 5 – 480x (depending on eyepiece and objectives)
3.15-945x (12.5-472x) 3.75-810x (19-405x)
episcopic illuminator is attached) (with coaxial episcopic illuminator: 15 – 540x) (coaxial episcopic illuminator: 22.5 – 540x)

Tubes P2-TERG 100 Trinocular Tilting Tube, P2-TERG 50 Trinocular Tilting Tube, P2-TL100 Trinocular Tube L P-B Binocular Tube, P-TL100 Trinocular Tube, P-TERG 100 Trinocular Tilting Tube, P-TERG 50 Trinocular Tilting Tube

P-B: 20°
Eyepiece inclination P2-TERG 100/50: 0°-30°, P2-TL100: 15° P-TL100: 15°
P-TERG100/50: 0°-30°
P2-TERG 100/50: 50 mm or wider P-B: 48–75mm
Interpupillary distance
P2-TL100: 50–75mm P-TL100: 50–75mm
adjustment
P-TERG100/50: 50 mm or wider

C-W10xB (F.N. 22), C-W15x (F.N. 16), C-W20x (F.N. 12.5), C-W30x (F.N. 7) C-W10xB (F.N. 22), C-W15x (F.N. 16), C-W20x (F.N. 12.5), C-W30x (F.N. 7)
Eyepieces
(with diopter adjustment) (with diopter adjustment)

Plan Apo 0.5x/WF, Plan Apo 0.75x/WF, Plan Apo 1x/WF, Plan Apo 0.5x/WF, Plan Apo 0.75x/WF, Plan Apo 1x/WF,
Objectives P2-SHR Plan Apo 0.5x, P2-SHR Plan Apo 1x, P2-SHR Plan Apo 1.6x, P2-SHR Plan Apo 2x
ED Plan 1.5x/WF, ED Plan 2x/WF ED Plan 1.5x/WF, ED Plan 2x/WF, Plan 1x, ED Plan 0.75x, Achro 0.5x

Working distance (with standard

60 mm 70 mm 78 mm
configuration or 1x objective)

32 kg
9.8 kg (with Binocular Tube + LED 11.9 kg (with Trinocular Tilting Tube +
Weight (approx.) (motorized Epi Fluorescence Attachment configuration) 10 kg (with Plain Stand and Ring LED set) 6.8 kg (with Binocular Tube + Plain Stand)
Diascopic Illumination Stand) LED Diascopic Illumination Stand)

* Depending on eyepiece and objective used

Greenough type

Model SMZ745/745T SMZ445 SMZ460 SMZ-2

Greenough type (zooming type)

Optical system Greenough type (zooming type) Greenough type (zooming type)
Trinocular Tube (SMZ745T)

Zoom ratio 7.5 : 1 4.4 : 1 4.3 : 1 5:1

Zoom range 0.67–5x 0.8–3.5x 0.7–3x 0.8–4x

Total magnification* 3.35–300x 4–70x 3.5–60x 4–120x

Fixed (binocular tube: SMZ745,

Tubes Fixed Fixed
trinocular tube: SMZ745T)

Eyepiece inclination 45° 45° 60° 45°

Interpupillary distance
52–75mm 54–75mm 54–75mm 56–75mm
adjustment

C-W10xB (F.N. 22), C-W15x (F.N. 16), C-W20x (F.N. 12.5), C-W30x (F.N. 7) SM E10xA (F.N. 23, standard), SM E15xA (F.N. 14), SM 20xB (F.N. 12),
Eyepieces SM 10xB (F.N. 21), SM 15xB (F.N. 14), SM 20xB (F.N. 12) SM 10xB (F.N. 21), SM 15xB (F.N. 14), SM 20xB (F.N. 12)
(with diopter adjustment) C-W30x (F.N. 7)

Objectives — — — 0.8-4x

G-AL 0.5x (W.D. 211mm), 0.7x (W.D. 150mm), 1.5x (W.D. 61mm), AL5 (0.5x, W.D. 103mm), AL7 (0.7x, W.D. 95mm)
Auxiliary objectives SM-AL 0.5x, 0.7x SM-AL 0.5x (W.D. 181mm), 0.7x (W.D. 127.5mm)
2x (W.D. 43.5mm)
Working distance (with standard
115mm 100mm 100mm 77.5mm
configuration or 1x objective)

1.6kg (SMZ745 body)

Weight (approx.) 1.0kg (body) 1.1kg (body) 1.6kg (body), 1.9kg (Stand)
1.8kg (SMZ745T body)
* Depending on eyepiece and objective used

28 29
Related Products
Digital Cameras for Microscopes Digital Microscope

High-definition color camera head

Camera Head Easy imaging

Microscope Camera
5.0-megapixel Color High-resolution
NEW Step. 1 Turn on the power.
High-definition cooled color camera head
16.25-megapixel Color High-resolution ShuttlePix provides 20x Step. 2 Adjust magnifications
optical zoom. Its magnification and focusing while observing
Monochrome Microscope Camera 5.0-megapixel Color Cooled High-resolution the monitor.
information is also linked to
NEW
High-speed color camera head ShuttlePix's scale and simple Step. 3 Press the image
16.25-megapixel Monochrome Cooled measurement functions. capture button.
* DS-Ri2 and DS-Qi2 do not require DS-U3 and DS-L3. 2.0-megapixel Color

PC-use Control Unit Enables a wide range of advanced digital

imaging capabilities using a PC One touch EDF imaging
＋

EDF（Extended Depth of Focus） option: Multichannel (multicolor)

Captures multiple high-resolution images at different focal depths to create a Multiple fluorescent channels can be captured in conjunction with other imaging
single extended depth of focus image or quasi-3D image. methods, such as OCC or brightfield.

Begin imaging from a low sample Finish imaging at a high sample

area on the screen. area on the screen.

Others

Images captured Handy set Simple stand set

in single colors • A cordless body (built-in illuminator, compatible with • Simple reflection stand that requires no battery
Overlapping image with all colors
SD card, battery-powered) • Diascopic LED stand enables diascopic
Individual cells resolved in a live drosophila embryo expressing GFP
and mCherry
• Easy operation imaging
Select the in-focus area and produce one
all-in-focus image (Using SHR Plan Apo 2x at zoom magnification of 8x with SMZ25) • Automatically uploads images to a PC
Image courtesy of Max V. Staller, Ph.D., Clarissa Scholes, and Angela DePace, Ph.D.,
Harvard Medical School
Time lapse

NIS-Elements makes it easy to set up a 0 sec 38.38 sec 57.14 sec 71.43 sec Calcium-imaging: Time-lapse imaging of Multi-purpose Zoom Microscope
time-lapse imaging experiment. GCaMP expressing neurons inside a live
zebrafish shows individual neurons firing at
different times (arrowheads). The last time-
frame shows a whole cluster of neurons • Wide magnification range
(Using SHR Plan Apo 2x at zoom magnification firing (asterisk). • Various observation methods in the macro region are possible
of 9x with SMZ25 and camera head DS-Qi1)
Image courtesy of Joe Fetcho, Ph.D., Cornell depending on samples and purpose.
University

Standalone Control Unit Offers an easy-to-use high-definition, large-touch-panel monitor Wide magnification range Various observation methods
that can be used to quickly capture images without the use of a
PC or monitor. 0.5x, 1x, 2x, 4x and 5x objectives are available. Used in combination with The AZ series mono-zoom mechanism enables true on-axis image
the AZ-W10x eyepiece and a coaxial episcopic illuminator, the AZ100 capture in the macro region. The AZ series supports a wide array of
series covers the full range of 5x to 500x magnifications. observation methods, including epi-fluorescence, reflected/transmitted
Scene mode Various tools
light brightfield, simple POL and differential interference contrast
Optimal imaging parameters for each sample type and observation method can Simple measurements of acquired image are possible, allowing lines and
easily be set using the icons. comments to be added to image data. In addition, data storage and output
functions for a wide range of applications are available.
Measurement function
Scene mode (bioscience) Scene mode (industrial)
Darkfield/fluorescence Wafer/IC-chip
Distance between Circle (perimeter/
Distance Perpendicular circle centers Angle Area Pitch distance
Differential interference/phase contrast Metal/ceramic diameter)

Board Scale display/positioning functions Drawing functions

Brightfield
HE staining FPD
Free curved Episcopic DIC Epi-fluorescence Diascopic simple Epi-fluorescence
SCALE XSCALE GRID XY MEAS X HAIRS Count mark Text input
Enzyme labeled antibody method line
polarizing and diascopic DIC

30 31
Optical Systems Parallel-optics type Greenough type
Parallel-optics type
(zooming type)
This system has a parallel optical path Eyepieces
DS series
into which various intermediate tubes, digital camera Eyepieces
including a beam splitter, coaxial
episcopic illuminator, epi-fluorescence
attachment, teaching head, drawing tube Prisms
and eye-level riser, can be inserted.

Greenough type Prisms

(zooming type)
Allows a compact body that is suited for
incorporation into other devices.
Zooming mechanism

Objectives Objectives

Image used in page 2 composite image courtesy of Julie C. Canman, Ph.D., Columbia University

N.B. Export of the products* in this catalog is controlled under the Japanese Foreign Exchange and Foreign Trade Law. Appropriate export procedure
shall be required in case of export from Japan.
*Products: Hardware and its technical information (including software)

Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. September 2014.
©2007-14 NIKON CORPORATION

WARNING TO ENSURE CORRECT USAGE, READ THE CORRESPONDING MANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT.

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Printed in Japan (1409-06)T Code No. 2CE-TVVH-9 This brochure is printed on recycled paper made from 40% used material.
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