2. Incubate 3mins at 40±2℃.

Aflatoxin M1 Rapid Test for Milk 3.

4.
Insert the dipstick into the microwell after first incubation. Incubate another 4mins at 40±2℃.
Take out the dipstick from the microwell and remove the sample pad at the lower end and
Order Code: YRM1004-3 then interpret the result.
Introduction
This rapid test is used for detection of Aflatoxin M1 in milk based on the colloidal gold Test Interpretation
immunochromatography technology. It takes about 7mins for one test. Visual Interpretation
1. Check whether the top control line(C line) is present. If there is normal C line, compare the
Application color intensity of test line (T line) and C line and interpret the test based on following chart.
1. Raw milk, pasteurized milk and full cream milk powder.
Test Line
2. Milk from cow, buffalo, ewe, goat, mare. Result
VS Result Analysis
Interpretation
Control Line
Performance Information
Sensitivity: limits of detection(ng/ml-ppb) The sample contains no Aflatoxin M1 or
T>C NEGATIVE contains residue at lower level than the
Residue Name LOD detection limits

Aflatoxin M1 0.3-0.4 The sample contains Aflatoxin M1 close to the

T=C WEAK POSITIVE
detection limits

The milk sample contains Aflatoxin M1 above

Storage and Shelf Life T <C or NO T POSITIVE
the detection limits
Storage: Store at 2-8℃. Do not freeze. Keep away from direct sunlight, moisture and heat.
Shelf Life: 18 months.
2. If there is no visible C line, the test is judged as invalid.
Test Kit Components Interpretation by Reader
1. 12 test tubes, each containing 1 strip of 8 red reagent microwells and 8 dipsticks. 1. Please refer to the reader instruction manual.
2. 1PC pipette(200μL), 100PCs pipette tips. 2. Negative:R>1.1, Weak positive:0.9≤R≤1.1, Positive: R<0.9.
3. Positive standards and negative standards. Interpretation diagram
4. 1 instruction manual.

Materials Required but not provided(available from Bioeasy)

1. Incubator capable of maintaining a temperature at 40±2℃.
2. Reader(optional).
3. Plate holder, Timer(optional).

Test Preparation
1. Connect the incubator and wait until the temperature has stabilized at 40℃±2℃.
2. Get the kit from refrigerator and allow the test tube warm up to room temperature(15-30℃).
3. Take required number of microwells and dipsticks from test tube.
4. Mix milk sample well to be homogeneous before testing.
5. Milk powder should be diluted by water at a ratio of 1:9 (e.g. 10 g milk powder diluted with 90
mL distilled water, and mix it well before testing).

Test Procedure
1. Pipette 200μL milk sample into the reagent microwell and mix well by pipetting up and down
5-10 times.

Shenzhen Bioeasy Biotechnology Co., Ltd.

ADD: No. 2-1, 1st Liuxian Street, Xin'an Road, Baoan District, Shenzhen, Guangdong, China，518101 TEL: +86-4001111126 /+86-755-27948546 FAX:+86-755-27948417 Email: info@bioeasy.com Web: www.bioeasy.com
V17-09-11
Negative and Positive Standards Reconstitution
Negative standards: Add 200μL distilled water into the microwell and mix well to be homogeneous, Test Procedure
then the sample will be ready for testing.
Positive Standards: Add 200μL negative milk into the microwell and mix well to be homogeneous,
then the sample will be reconstituted in the concentration stated on the label. Customers may
further dilute the sample to desired concentration by negative milk or directly use it for testing.
Note: Both the negative and positive reconstituted standards should be transferred to the red
reagent microwell and follow the steps specified in test procedure thereafter for testing.

Precautions
1. It is advisable to use a clean table and wash hands thoroughly before testing to avoid any
contamination of the test which is very sensitive to antibacterial substances.
2. The milk sample must be homogeneous and in liquid without clot or sediment. Samples
should be mixed completely before detection.The ideal sample temperature is 20-25°C.
3. Do not mix use reagent microwells and dipsticks from different lots. Use the kit before it is 1. Pipette 200μL milk sample into the reagent 2. Put the microwell on the incubator and
expired. microwell and mix well by pipetting up and down incubate 3mins at 40±2℃.
4. The tube with microwells and dipsticks should always be well closed after reagents have been 5-10 times.
taken out. Empty one tube before opening another and try to finish one tube within a week.
5. Use a new pipette tip for every new sample.
6. Pipette the milk samples gently to avoid the milk samples rush into the pipette hole and
possible contamination of the pipette by positive samples.
7. Hold the dipstick from the upper side (Absorbing pad side). Do not touch the lower end
(Sample pad and Nitrocellulose membrane areas), which may affect the performance of the
dipsticks.
8. After the second incubation, read the result directly within 5mins. The results is invalid after
more than 5mins.
9. If the fat content in the sample is high, the dipstick chromatography speed will be lower and
reagents flow more slowly towards to upper end. It is recommended to extend the second
incubation by 60 seconds in this condition. 3. Insert the dipstick into the microwell after first 4.Take out the dipstick from the microwell and
10. When a positive result is identified, repeat testing for double confirmation. incubation. Incubate another 4mins at 40±2℃. remove the sample pad at the lower end and
11. If there is obvious breakpoint on the Test line, repeat the test. then interpret the result.
12. This product is only used for preliminary screening, and the final result shall be subject to the
official arbitration detection methods.

Shenzhen Bioeasy Biotechnology Co., Ltd.

ADD: No. 2-1, 1st Liuxian Street, Xin'an Road, Baoan District, Shenzhen, Guangdong, China，518101 TEL: +86-4001111126 /+86-755-27948546 FAX:+86-755-27948417 Email: info@bioeasy.com Web: www.bioeasy.com
V17-09-11