SnapShot: Extracellular Vesicles

Federico Cocozza,1,2,3 Eleonora Grisard,1,3 Lorena Martin-Jaular,1,3 Mathilde Mathieu,1,2,3 and Clotilde Théry1,3
1
Institut Curie, INSERM U932, PSL Université, 26 rue d’Ulm, Paris 75005, France
2
Université de Paris, 85 Bd St germain, Paris 75006, France
3
These authors contributed equally
1 EV subtypes 2 EV cargoes

Multivesicular Recycling Plasma membrane

body endosome protrusion Proteins

Lipids
AAA
AAA

Nucleic acids
AA
AA
AA

Exomeres Enveloped virus Exosomes Microvesicles/ectosomes/

(30–50 nm) (80–400 nm) (50–150 nm) Small EVs microparticles/oncosomes/
apoptotic bodies (50 nm–≥5 μm)

3 Budding mechanisms 5 Concentration and isolation methods

Lipids Key Precipitation (P) Filter concentration (FC)

flipping PEG
Large EV
Tetraspanins
P Medium EV
FC Small EV Filter
Ceramide Spin Spin
Exomere
Proteins/
SEC aggregates
ESCRT
Cytoskeleton
Size-exclusion chromatography (SEC) Differential ultracentrifugation (dUC)
DG

Recovery
dUC

262 Cell 182, July 09, 2020 ©2020 Elsevier Inc.  DOI https://dx.doi.org/10.1016/j.cell.2020.04.054
4 Functions
AF4
SECRETING
CELL Intracellular Spin Spin Spin
components IP
disposal SN SN

Circulation
into vessels
Specificity Increasing speeds

Density gradients (DG) Asymmetric flow field-flow fractionation (AF4) Immunoprecipitation (IP)
Interaction Density Bottom-up Top-down Flow
with through
extracellular Channel flow in Channel flow out
matrix
Latex or
Spin Spin magnetic
Cargo Spin or
beads and
transfer Interaction with
antibody
recipient cell via IP
extracellular matrix Parabolic Cross flow out
flow profile
Receptor-mediated
RECIPIENT CELL
signalling
Cell, 182 (2020) 262-262.e1. doi:10.1016/j.cell.2020.04.054