A046 R1 WAD Cyanide ISO Dist

AutoAnalyzer 500
Method no. A-046-19 Rev. 1 www.seal-analytical.com
WAD Cyanide in Water (ISO distillation method)
1 SCOPE
This method covers the determination of WAD Cyanide in Water and Wastewater. WAD (= Weak Acid
Dissociable) Cyanide is the sum of free cyanide ions and weak cyanide complexes from cadmium,
zinc, silver and copper. Organically bound cyanides and thiocyanate are not included.
2 CALIBRATION RANGE
Low Range: 5 – 50 µg/L as CN High Range: 100 – 1000 µg/L as CN
3 TYPICAL PERFORMANCE DATA
Low range High Range

Sampling 25 samples/h, 3:1 25 samples/h, 3:1
Highest Calibrant 50 µg/L 1000 µg/L
Blank variation 0.03 µg/L -
(SD of 10 sequential blanks)
Detection limit 0.4 µg/L -
(DIN 32645)
Reporting limit 0.6 µg/L -
(DIN 32645)
These performance statistics were generated using genuine SEAL Analytical parts and consumables. Performance may vary
depending on system components and the number of channels selected.
Performance values are pooled from independent runs. Refer to section 16 for details.
4 METHOD PRINCIPLE
The hydrogen cyanide present at pH 3.8 is separated from by online distillation at 125°C. The
condensate is reacting with chloramine T at pH 5.2 to form cyanogen chloride which reacts with
1,3-Dimethylbarbituric acid and isonicotinic acid. The blue compound that is formed this way is
determined photometrically at 600 nm
5 REFERENCES
1. ISO EN DIN 14403-2:2012

2. ISO 17380
6 HARDWARE REQUIREMENTS
Chemistry Hardware: 2.0 mm glass, 2 mL distillation heating bath, 7 mL heating bath

Detector: LED photometer at 600 nm,10 mm flowcell
Pump tubes: 13 + 1 air (2s) + 1 air (4s) + sampler wash
AutoAnalyzer 500 Method no. A-046-19 Rev. 1 , page 2/23
7 LIST OF REQUIRED CHEMICALS
All chemicals must be of analytical grade quality (ACS grade, pro analysi, …). DI water refers to high
grade quality distilled or deionized water, free from organic contamination (e.g. ISO 3696 Grade 1 or
ASTM standard D 1193 Type I/II)
Persons using this method should be familiar with normal laboratory practice. This method does not
purport to address all safety risks, if any, associated with its use. It is the responsibility of the user to
establish safety and health practices and to ensure compliance with local regulatory conditions.
Compound CAS No. Safety classification

Brij-35, 22-30% solution 9002-92-0 --
Chloramine-T trihydrate 7080-50-4 GHS05, GHS07, GHS08
Citric acid monohydrate 5949-29-1 GHS07
1,3-Dimethylbabituric acid 769-42-6 GHS04, GHS07
Ethanol, C2H6O 64-17-5 GHS02
Hydrochloric acid, 36.5-38% 7647-01-1 GHS05, GHS07
Potassium cyanide 151-50-8 GHS06, GHS09
Potassium hexacyanoferrate(III) 13746-66-2 --
Potassium hydrogen phthalate 877-24-7 --
Potassium tetracyanozincate solution 14244-62-3 GHS06, GHS09
1000 mg/L as CN
Potassium thiocyanate 333-20-0 GHS07

Pyridine-4-carboxylic acid
55-22-1 GHS07
(isonicotinic acid)
Sodium hydroxide 1310-73-2 GHS05
Zinc sulfate heptahydrate 7446-20-0 GHS05, GHS07, GHS09
GHS01: Danger - Explosive; GHS02: Danger - Flammable; GHS03: Danger - Oxidizing; GHS04: Warning - Compressed
gas; GHS05: Warning/Danger - Corrosive; GHS06: Danger - Toxic; GHS07: Irritant; GHS08: Danger - Health hazard;
GHS09: Warning/Danger - Environmentally Damaging
8 REAGENT PREPARATION
To reach the stated performance values, standard, reagents and sampler wash must be free of solids
and dissolved air.
For best performance vacuum filter all reagents through a 45 µm filter or glass filter paper. If necessary,
vacuum filter all DI water used in the preparation of standards and for the sampler wash or degas the
water in another way.
The total volume of each reagent can be varied, if the concentrations of its ingredients remain the
same. It is recommended to only prepare the required amount of reagent, which can be calculated
from the flowrate of its corresponding reagent pump tube.
8.1 SAMPLER WASH SOLUTION – SODIUM HYDROXIDE 0.01 mol/L
Raw material Amount Reagent Label

Sodium hydroxide 0.4 g
DI water to 1000 mL
Storage: Plastic bottle at room temperature Warning
Stability: one month if pH is stable
Dissolve 0.4 g of sodium hydroxide in about 800 mL of DI water. Dilute to 1000 mL with DI water and
mix thoroughly.
Copyright 2019 by SEAL Analytical. Not to be reproduced without permission.

8.2 SYSTEM WASH SOLUTION

Brij-35, 22-30% solution 1 mL
DI water to 1000 mL
N/A
Storage: Glass or plastic bottle at room temperature
Stability: Infinite, replace if turbid or cloudy (recommended: weekly)
Add 1 ml Brij-35 solution to about 800 mL of DI water. Dilute to 1000 mL with DI water and mix
thoroughly.
8.3 SODIUM HYDROXIDE 0.4 mol/L

Sodium hydroxide 16 g
DI water to 1000 mL
Stability: Infinite, replace if turbid or cloudy
Dissolve 16 g of sodium hydroxide in about 700 mL of DI water. Dilute to 1000 mL with DI water and
mix thoroughly.
8.4 SODIUM HYDORXIDE 1 mol/L

Sodium hydroxide 40 g
DI water to 1000 mL
Dissolve 40 g of sodium hydroxide in about 700 mL of DI water. Cool to room temperature. Dilute to
1000 mL with DI water and mix thoroughly.
8.5 HYDROCHLORIC ACID 1 mol/L

Hydrochloric acid, 36.5 – 38% 85 mL
DI water to 1000 mL
Add 85 mL of hydrochloric acid to about 700 mL of DI water. Cool to room temperature. Dilute to
1000 mL with DI water and mix thoroughly.

8.6 DISTILLATION REAGENT

Citric acid monohydrate 20 g
Sodium hydroxide 0.4 mol/l 100 mL
Sodium hydroxide 1 mol/L as required
Hydrochloric acid 1 mol/l as required
N/A
Hydrochloric acid 1 mol/l 25 mL
DI water to 1000 mL
Storage: Plastic bottle at 4°C
Stability: one week, check pH daily
Dissolve 20 g of citric acid monohydrate in about 700 mL of DI water. Add 100 mL of sodium hydroxide
solution 0.4 mol/L. Mix thoroughly and adjust the pH to 3.8 with hydrochloric acid 1 mol/L and/or
sodium hydroxide 1mol/L. Add 25 mL of hydrochloric acid 1 mol/L, dilute to 1000 mL with DI water and
mix thoroughly.
Note: Due to the extra addition of hydrochloric acid the pH of this reagent will be 3.4. After mixing with
the sample a pH of 3.8 is achieved.
8.7 DILUTION WATER

Use DI water without surfactant.
8.8 ZINC SULFATE SOLUTION

Zinc sulfate heptahydrate 10 g
DI water to 1000 mL
N/A
Storage: Glass or plastic bottle at room temperature
Dissolve 10 g of zinc sulfate in about 700 mL of DI water. Dilute to 1000 mL with DI water and mix
thoroughly.
8.9 PHTHALATE BUFFER

Potassium hydrogen phthalate 20.5 g
Sodium hydroxide 1 mol/l as required
Hydrochloric acid as required
N/A
DI water to 1000 mL
Storage: Amber glass or plastic bottle at 4°C
Stability: one month
Dissolve 2.3 g of sodium hydroxide in about 500 mL of DI water. Add 20.5 g of potassium hydrogen
phthalate and dissolve completely. Dilute to 975 mL with DI water. And mix thoroughly. If necessary
adjust the pH to 5.2 with hydrochloric acid 1 mol/L and/or sodium hydroxide 1 mol/L. Dilute to 1000 mL
with DI water, add 1 mL of Brij-35 solution and mix thoroughly.

8.10 CHLORAMINE T

Chloramine T trihydrate 2.0 g
DI water to 1000 mL
N/A
Stability: one week
Dissolve 2.0 g of chloramine T in about 800 mL of DI water. Dilute to 1000 mL with DI water and mix
thoroughly.
8.11 COLOR REAGENT

1,3-dimethylbarbituric acid 16.8 g
Pyridien-4-carboxylic acid (isonicotinic acid) 13.6 g
Sodium hydroxide 1 mol/l as required
Hydrochloric acid 1 mol/l as required
DI water to 1000 mL Warning
Dissolve 7.0 g of sodium hydroxide in about 500 mL of DI water. Add 16.8 g of 1,3-dimethylbarbituric
acid and 13.6 g of isonicotinic acid. Dilute to 950 mL with DI water and mix thoroughly. f necessary
adjust the pH to 5.2 with hydrochloric acid 1 mol/L and/or sodium hydroxide 1 mol/L. Dilute to 1000 mL
with DI water and stirr for one hour at 30°C. Filter the solution, add 1 mL of Brij-35 solution and mix
thoroughly.

9 STANDARDS
These formulas assume that samples are preserved with the described preserving solution (see
section 10). If other preservation methods are used, the sample and standard matrix and the sampler
wash solution should be similar.
9.1 STOCK STANDARD A, 100 mg/L as CN

Potassium cyanide 250 mg
Sodium hydroxide 1 mol/l 10 mL
Di water to 1000 mL
Storage: amber glass or plastic bottle at 4°C Warning
Add 10 mL of sodium hydroxide 1 mol/L to about 600 mL of DI water and mix thoroughly. Add 250 mg
of potassium cyanide and dissolve completely. Dilute to 1000 mL with DI water and mix thoroughly.
NOTE: Potassium cyanide is highly toxic.
9.2 STOCK STANDARD B 5 mg/l as CN

Sodium hydroxide 1 mol/L 1 mL
Stock standard A, 100 mg/L as CN 5 mL
DI water to 100 mL
Storage: amber glass or plastic bottle at 4°C Warning
Stability: one week
Add 1 mL of sodium hydroxide solution 1 mol/L to about 40 mL of DI water and mix thoroughly. Add
50 mL of stock standard A and dilute to 100 mL with DI water. Mix thoroughly.
9.3 STOCK STANDARD C 50 mg/l as CN

Sodium hydroxide 1 mol/L 1 mL
Potassium tetracyanozincate solution
5 mL
1000 mg/L as CN
DI water to 100 mL
Warning
Storage: amber glass or plastic bottle at 4°C
Stability: one week
Add 1 mL of sodium hydroxide solution 1 mol/L to about 60 mL of DI water and mix thoroughly. Add
5 mL of potassium tetracyanozincate solution and dilute to 100 mL with DI water. Mix thoroughly.

9.4 WORKING STANDARDS

Prepare working standards fresh every day before use. For example:
mL of Stock C µg/L as CN mL of Stock C µg/L as CN

0.1 50 2 1000
0.075 37.5 1.5 750
0.05 25 1 500
0.025 12.5 0.5 250
0.01 5 0.2 100
Pipette each aliquot of Stock standard C into a 100 mL volumetric flask. Dilute to volume with 0.01N
sodium hydroxide solution and mix thoroughly.

10 SAMPLE PRESERVATION AND STORAGE
Immediately after sampling adjust the pH of water samples to 12 with sodium hydroxide solution.
Choose a suitable concentration for the sodium hydroxide solution, so that the dilution of the samples
is negligible.
If necessary remove larger particles (> 0.1 mm) by filtration or decantation at the laboratory. Test for
interferences and treat if necessary as described in section 11.
Samples should be analyzed as soon as possible but at least within three days.
Store samples in a cold dark place.
11 INTERFERENCES
According to ISO EN DIN 14403-2:2012:
Oxidizing agents such as chlorine decompose most of the cyanides. If the presence of oxidizing
agents cannot be excluded, treat the sample immediately after sampling. Test a drop of the sample
with potassium iodide starch test paper. A blue color indicates the need for treatment. Add sodium
thiosulfate (a few crystals at a time) until a drop of sample does not produce color on the indicator
paper anymore. If the samples are not measured within the next 24 hours add an additional portion of
0.6 g ascorbic acid for each 1000 mL of sample volume.
Interference of sulfide starts at 100 mg/L. Test a drop of the sample with lead acetate paper. If this
indicates the presents of sulfides treat a portion of the stabilized sample with powdered lead acetate.
Lead sulfide will precipitate if sulfide is present in the sample. Repeat this operation until the lead
acetate paper does not indicate the presence of sulfides anymore. Filter the sample through a dry filter
paper and use the filtrate for measuring. Avoid large excess of lead and long contact times to avoid
complexation or occlusion of cyanide to the precipitate.
Aldehydes and ketones can (under certain conditions) absorb cyanide by nucleophilic addition. To
avoid this interference ethylenediamine can be added to the sample.
Under the given distillation conditions aldehydes can transform cyanide to nitrite. Aldehydes can be
removed by adding silver nitrate to the sample. The addition can alter the ration between Total Cyanide
and WAD Cyanide. The user should evaluate this process.
Interference by nitrite above concentrations of 5 mg/L can be avoided by addition of sulfamic acid to
the distillation buffer.
Sulfite interferes above concentrations of 1 mg/L.
Salt concentrations higher than 10 g/L can cause blocking of the distillation coil. Dilute these samples
prior to measurement.
Thiocyanate can slightly interfere and lead to positive bias. Significant interferences can arise from
cyanide impurities in thiocyanate or from inappropriate distillation procedures.
12 START-UP PROCEDURE
1. Switch on all modules

2. Start the cooling water supply
3. Start a charting to heat up the distillation heating baths
4. When the temperature has reached at least 100°C, start pumping:
- DI water through the reagent lines for sampler wash, distillation reagent and dilution
water.
- System wash solution through the other reagent lines.
5. Wait for a stable bubble pattern
6. Switch the all reagent lines except for the zinc sulfate solution line from wash solution to their
corresponding reagent
7. When the distillation reagent reaches the distillation, switch the zinc sulfate line to its reagent.
8. Wait for the baseline to stabilize again.

13 SHUTDOWN PROCEDURE
1. Switch the reagent lines:

- DI water through the reagent lines for sampler wash, zinc sulfate and dilution water.
- Distillation reagent through its reagent line
2. Pump for at least 15 minutes
3. Switch the distillation reagent line to DI water.
4. Switch off the heating baths
5. Wait until the distillation heating bath has cooled down to less than 100°C.
6. Place all reagent lines in 1N NaOH for ten minutes.
water.
8. Pump for at least ten minutes
9. Place all reagent lines in 1N HCl for five minutes.
water.
11. Pump for at least 20 minutes
12. Aspirate air through the reagent lines for sampler wash, distillation reagent and dilution water
until the distillation heating bath is completely empty.
13. Release the pump platen
14. Switch of all modules.
14 SYSTEM CLEANING PROCEDURE
DAILY:
Follow the shutdown procedure (see section 13).
WEEKLY – SPECIAL CLEANING FOR THE DISTILLATION COIL:

1. Perform the shutdown procedure but stop after step 10.
2. Disconnect the resample tubing between distillation part and the wht/wht resample pump tube
3. Connect a plastic syringe filled with app. 20 mL 1N HCl to the transmission tubing
4. Disconnect the red/red distillation waste pump tube from its transmission line and place the line
in an empty beaker.
5. Flush the HCl from the syringe into the distillation coil so that the coil is completely filled.
6. Release the pump platen and let the solution stand in the coil overnight.
7. Flush out the HCL with at least 20 mL of DI water.
8. Empty the distillation coil.
9. Reinstall the tubing connections.

15 OPERATING NOTES
1. The distillation heating bath should have a temperature of 125 ± 1°C.
2. The determination of Total Cyanide requires a UV digestion step and is described in method A-
016. It is possible to measure WAD cyanide on a Manifold with method A-016, by switching of
the UV digestor and connecting the reagents as shown in the flowchart in section 19 of this
method. The flowrates on both methods are identical.
3. Due to the addition of hydrochloric acid the pH of the distillation reagent will be app. 3.4. After
mixing with the preserved sample, a pH of 3.8 will be achieved. The pH of the distillation reagent
is very important and has to be adjusted carefully. Incorrect pH values will decrease the recovery
rate for total cyanide. If the samples and standards contain more than 0.01 mol/L sodium
hydroxide, sufficient HCl must be added to the distillation reagent after pH adjustment to
neutralize the excess of alkali in the samples.
4. Do not store the color reagent at temperatures below 2°C, as this can cause precipitation of this
solution.
5. A condenser with high efficiency is needed to obtain a low noise signal in the low range. Cooling
water should have a temperature between 9 and 12°C and the cooling coils must be filled
completely with cooling water. A refrigerated circulation bath is recommended.
6. Glass to glass connections at the distillation step and the UV digestor should be made with
Acidflex tubing (P/N 116-0538-15).
7. The distillation heating bath has to be empty before heating up. Otherwise the evaporation of
the water can cause increased pressure inside the system that will make the system burst. .
8. The daily cleaning procedure (steps 4 – 9 of the shutdown procedure) can also be performed
before starting up the system. In this case do not heat up the distillation and empty the distillation
part again before continuing with the start-up procedure.
9. Waste solutions can contain low concentrations of cyanide ions. Therefore it is recommended
to place some 1N NaOH in the waste containers to prevent liberation of HCN gas. Some
phenolphtaneline solution should be added to control the alkaline conditions in the container.
10. To avoid salt precipitation in the distillation coil due to samples with high salt content or the zinc
sulfate, the dilution water for the distillation can be replaced with a 30% glycerol solution.
This changeover is not covered by ISO 14403-2:2012!

16 PERFORMANCE VALIDATION
16.1 TEST CONDITIONS
Sampler type XY3 sampler

Sample probe HiTech 0.75 mm
Sample tubing and length PE15, 120cm
Total sample flow rate 2.20 ml/min
(incl. by-pass and other channels)
Wash pot type Fixed
Wavelength 600 nm
Reference energy
Sample:ref ratio
Light power 98%
Room temperature 22 – 25°C
16.2 PERFORMANCE DATA – OVERVIEW
Low range High range

Highest Calibrant 50 µg/L 1000 µg/L
Sensitivity 0.045 – 0.055 AU 0.83 – 1.0 AU
(in specified flowcell)
Correlation Coefficient 0.999 0.999
(linear, six points)
Reagent absorbance 0.01 – 0.02 AU
Coefficient of Variation 0.10 µg/L, 0.4% 2.95 µg/L, 0.6%
Pooled SD 0.75 µg/L

Blank variation 0.028 µg/L
(SD of 10 sequential blanks)
(DIN, blank method)
(DIN, calibration method)
(DIN, blank method)
(DIN, calibration method)
These performance statistics were generated using genuine SEAL Analytical parts and consumables.
Performance may vary depending on system components and the number of channels selected.
Performance values are pooled from independent runs. The bold values are reported on the first page of this method
document.

16.3 CALIBRATION DATA
Low calibration range: 5 µg/L to 50 µg/L as CN. Absorbance of top standard: 0.046 AU
High calibration range: 100 µg/L to 1000 µg/L as CN. Absorbance of top standard: 0.92 AU

16.4 REPRODUCIBILITY – SAME CONCENTRATION
The reproducibility is checked by measuring 20 replicates of a 50% calibration range standard. Three
runs are performed on three different days. Baseline and sensitivity drift correction are applied.
The coefficient of variation is calculated by dividing the standard deviation of the replicates by the
mean and then multiplying with 100.
Peak no. Low Range High Range
µg/L as CN
Nominal 25 500
1 24.950 498.621
2 25.042 494.994
3 24.911 497.378
4 24.995 493.266
5 25.046 498.364
6 25.100 496.007
7 24.958 498.606
8 24.961 496.807
9 25.009 495.193
10 25.126 491.809
11 25.041 490.881
12 25.112 492.266
13 25.109 495.936
14 25.207 494.265
15 25.100 490.810
16 25.272 492.794
17 25.158 490.810
18 25.048 491.295
19 25.232 491.281
20 25.134 489.596
Mean 25.076 494.049
Std. deviation 0.098 2.948
Coefficient of variation 0.39% 0.60%

16.5 REPRODUCIBILITY – VARYING CONCENTRATION
Reproducibility is checked by running ten replicates of five different standards in pseudo-random order.
Three runs are performed on three different days. Baseline, sensitivity drift and carryover correction
are applied.
Low Range High Range
Nominal Nominal
Standard deviation Standard deviation
Group concentration concentration
µg/L as CN µg/L as CN
µg/L as CN µg/L as CN
1 50.0 1.299 1000 5.029
2 37.5 0.907 750 14.020
3 25.0 1.992 500 18.762
4 12.5 0.283 250 10.975
5 0 0.176 0 0.227
Pooled SD 0.753 Pooled SD 11.784
16.6 DETECTION AND REPORTING LIMIT DATA (DIN BLANK METHOD)
The DIN limits are determined from ten replicates of blanks. Three runs are performed on three
different days. Baseline and sensitivity drift are applied. The calculation is based on DIN 32645:2008.
Peak no. Low Range
Raw digits
1 3225
2 2343
3 3251
4 3251
5 3245
6 3266
7 3328
8 3281
9 3259
10 3254
Decision Limit 0.05 µg/L
Detection limit 0.09 µg/L
Reporting Limit 0.20 µg/L

16.7 DETECTION AND REPORTING LIMIT DATA (DIN CALIBRATION METHOD)
The DIN limits are determined from a 10 point calibration according to DIN 32645:2008. Three runs
are performed on three different days. Baseline and sensitivity drift are applied. A linearity Test
according to ISO 8466-1 is applied.
Nominal concentration
x = Raw digits
y in µg/L
0.5 3724
1.0 4199
1.5 4738
2.0 5197
2.5 5727
3.0 6362
3.5 6487
4.0 7188
4.5 7760
5.0 8233
y = 1006x + 3219
Linear correlation, r2 R2=0.9990
y = -7.2x² + 1046x + 3180
Quadratic correlation, r2 R2=0.9981
PG 1.567
F 13.745
→ The F-Test supports a linear calibration curve
Decision limit 0.20 µg/L
Detection limit 0.40 µgL
Reporting limit 0.66 µg/L
Calibration Curve from test run

16.8 DETECTION LIMIT DATA (EPA SPIKE METHOD)
The detection limit MDLs is determined from ten replicates of spikes. Three runs are performed on
three different days. Baseline and sensitivity drift are applied. The detection limit is calculated by
multiplying the standard deviation of the replicates by the student factor for 10 replicates (T = 2.821).
Peak no.
µg/L as CN
Nominal 0.500
1 0.447
2 0.439
3 0.406
4 0.375
5 0.357
6 0.325
7 0.323
8 0.286
9 0.278
10 0.266
Mean 0.350
Std. deviation 0.066
Detection Limit 0.185
16.9 DETECTION AND REPORTING LIMIT DATA (EPA BLANK METHOD)
The detection limit MDLb is determined from ten replicates of blanks. Three runs are performed on
three different days. Baseline and sensitivity drift are applied. The detection limit is calculated by
multiplying the standard deviation of the blanks by the student factor for 10 replicates (T = 2.821) and
adding the mean value, if it is positive.
Peak no.
µg/L as CN
1 0.0056
2 0.0235
3 0.0314
4 0.0314
5 0.0254
6 0.0463
7 0.1079
8 0.0612
9 0.0393
10 0.0344
Mean 0.041
Std. deviation 0.028
Detection Limit 0.119

16.10 RECOVERIES
The recoveries are checked by running three replicates of reference substance standards. Three runs
are performed on three different days. Baseline and sensitivity drift correction are applied.
Substance Recovery Rate

Potassiumhexacyanoferrate(III) <5% at 100 µg/L
Potassiumthiocyanate <1% at 1000 µg/L
16.11 OTHER METHOD DATA AND SETTINGS
Parameter Value Notes

Time between sample cup and
Lag time 15 minutes detector. Depends on number of
channels in use.
Carryover 0.4% See AACE manual for calculation.
See AACE manual for how reagent
Reagent absorbance 0.01 – 0.02 AU
absorbance is calculated.
See AACE manual for further
Smoothing none
information.

16.12 TYPICAL PEAK SHAPES
16.12.1 LOW RANGE
Low range: Spike replicates at 0.5 µg/L (expanded view)
Low range: Excerpt from a test run at 5 concentrations

16.12.2 HIGH RANGE
High range: Excerpt from a test run at 5 concentrations

17 REVISIONS
Revision Date Changes

0 March 2019 New method
Logo changed, introduction for section 8 updated, flowchart
1 December 2019
and parts list updated, minor text changes.

18 PARTS LIST
Only use genuine SEAL parts and consumables with the SEAL logo or the “SEAL Tec” stamp on the
package or the part itself. Performance cannot be guaranteed if parts from other sources are used and
warranty might be lost If repairs are carried out with non-genuine spare parts or by unauthorized
personnel.
18.1 CONSUMABLES KIT – 12 MONTHS
Description Legend Part Number Kit Content

ORN/GRN, 0.10 mL/min 116-0549-04 3 pkg / 12
ORN/YEL, 0.16 mL/min 116-0549-05 1 pkg / 12
ORN/WHT, 0.23 mL/min 116-0549-06 3 pkg / 12
BLK/BLK, 0.32 mL/min 116-0549-07 2 pkg / 12
ORN/ORN, 0.42 mL/min 116-0549-08 1 pkg / 12
WHT/WHT, 0.60 mL/min 116-0549-09 4 pkg / 12
RED/RED, 0.80 mL/min 116-0549-10 1 pkg / 12
Tubing airbar silicone Si543 116-0543-01 2.4 m
18.2 ADDITIONAL TUBINGS
Description Legend Part Number Sales Unit

Tubing polyethylene PE02 562-2002-01 1m
Tubing polyethylene PE15 562-2015-01 1m
Tubing Tygon T06 116-0536-06 1m
Tubing transmission T528 116-0528-01 1m
Tubing reagent big, red R02B 116+1100-02 1m
Tubing reagent small, clear R01S 116+1101-01 1m
Tubing reagent small, blue R03S 116+1101-03 1m
Tubing reagent small, green R04S 116+1101-04 1m
Tubing reagent small, purple R05S 116+1101-05 1m
Tubing Acidflex AC09 116-0538-09 1m
Tubing Acidflex AC15 116-0538-15 1m
18.3 SPARES KIT
Description Legend Part Number Kit Content

Injection fitting, 3 pt. a 116-0489-01 1 pc
Glass coil, 5 turns 5TM 163+G001-05 1 pc
Glass coil, 5 turns + Nipple 5TM + NE 163+G003-05 1 pc
Glass coil 20 turns 20TM 163+G001-20 1 pc
Debubbler C3 116-0202-03 1 pc
Glass tubing S ak 170-G023-01 1 pc
Glass tubing L ar 157-0185-01 1 pc
Nipple tz tz 822508 1 pc
Nipple N5 N5 116-0002-01 6 pcs
Nipple N8 N8 116-0003-01 6 pcs
Nipple R13 R13 166+B152-01 6 pcs

18.4 PHOTOMETER PARTS

Flowcell 10 mm 169+B045-10 1 pc
Optic Assy 10 mm 161+B650-10 1 pc
LED Assy, 600 nm 161+B661-60 1 pc
Coil 7 turns 161+G500-01 1 pc
18.5 OTHER SPECIAL PARTS

Cooling coil 5 turns co 163+G031-05 1 pc
Phase separator small cv 163+G032-02 1 pc
Coil assy 8 turns (2.2 mL), fix 163+B410-31 1 pc
Coil assy 12 turns (7 mL), fix 163+B410-12 1 pc
Heating bath, fix (2.2 mL) 163+B405-22 1 pc
Heating bath, fix (7 mL) 163+B404-12 1 pc

19 FLOWCHART
NOTES : Cut resample pump tube to 1cm on both sides. PE15 orn/orn PE15
Resample PE tubing as short as possible. waste N8 N8
PE02 N8 Si543 blk/blk air

N8
5TM+NE 5TM+NE 5TM
cl a N5
wht/wht resample 2 C3
PE15 PE15
N8 N8
colorimeter Heating bath in RB93 blk/blk pthalate buffer R04S
600nm 7 ml 37 °C R13 R13
f / c: 1.0 x 10 mm
20TM out R05S orn/wht chloramine-T R05S
PE15 R13 R13
waste PE15
cl R01S orn/wht color reagent R01S
R13 R13
PE15
1,2m 562-0005-01 AC15 PE15 30cm

cooling water out waste
tz
PE15 red/red liquid trap
1,2m 562-0005-01 AC15 waste N8 N5
cooling water in cv AC15
tz
ar co
AC15
co ak
out AC15 12cm PE02 no air valve wht/wht air
R13
in
Dist. Heating bath
2.2 ml 125 °C cl a N5
PE15 wht/wht resample 1 PE15
C3
N8 N8
5TM+NE RB93 wht/wht dilution water R03B

N8 N8
orn/grn R13
ZnSO4 R02S
R13
R02S
PT19
R13
orn/grn R13
dilution water R02S
AC15 10cm protective hose PE15
PE02 N8 Si543 orn/yel air // 4 sec.

N8
5TM
a N5 sample
PE15 wht/wht PE15
N8 N8
R01S orn/grn distillation buffer R01S

R13 R13
DRAWN B. Dettmer 25.03.2019 SYSTEM

AA 500 PARAMETER WAD Cyanide
CHANGED P. F. Schulz 19.12.2019 METHOD NO.
(1) A-046-18 MATRIX Water and Waste Water
PROPRIETARY NOTE
This drawing contains information proprietary to SEAL Analytical
lowest range : 5 - 50 µg/L as CN and must be kept confidential.Reprints and disclosures are not
RELEASED S. Dorst 19.12.2019 REMARK RANGE
highest range : 100 - 1000 µg/L as CN permitted without written consent of SEAL Analytical

A046 R1 WAD Cyanide ISO Dist

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A046 R1 WAD Cyanide ISO Dist

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Method no. A-046-19 Rev. 1 www.seal-analytical.com

WAD Cyanide in Water (ISO distillation method)

Low Range: 5 – 50 µg/L as CN High Range: 100 – 1000 µg/L as CN

3 TYPICAL PERFORMANCE DATA

Low range High Range

1. ISO EN DIN 14403-2:2012

Chemistry Hardware: 2.0 mm glass, 2 mL distillation heating bath, 7 mL heating bath

7 LIST OF REQUIRED CHEMICALS

Compound CAS No. Safety classification

Potassium thiocyanate 333-20-0 GHS07

8.1 SAMPLER WASH SOLUTION – SODIUM HYDROXIDE 0.01 mol/L

Raw material Amount Reagent Label

Copyright 2019 by SEAL Analytical. Not to be reproduced without permission.

8.2 SYSTEM WASH SOLUTION

Raw material Amount Reagent Label

8.3 SODIUM HYDROXIDE 0.4 mol/L

Raw material Amount Reagent Label

8.4 SODIUM HYDORXIDE 1 mol/L

Raw material Amount Reagent Label

8.5 HYDROCHLORIC ACID 1 mol/L

Raw material Amount Reagent Label

Copyright 2019 by SEAL Analytical. Not to be reproduced without permission.

8.6 DISTILLATION REAGENT

Raw material Amount Reagent Label

8.7 DILUTION WATER

8.8 ZINC SULFATE SOLUTION

Raw material Amount Reagent Label

8.9 PHTHALATE BUFFER

Raw material Amount Reagent Label

Copyright 2019 by SEAL Analytical. Not to be reproduced without permission.

Raw material Amount Reagent Label

8.11 COLOR REAGENT

Raw material Amount Reagent Label

Copyright 2019 by SEAL Analytical. Not to be reproduced without permission.

9.1 STOCK STANDARD A, 100 mg/L as CN

Raw material Amount Reagent Label

9.2 STOCK STANDARD B 5 mg/l as CN

Raw material Amount Reagent Label

9.3 STOCK STANDARD C 50 mg/l as CN

Raw material Amount Reagent Label

Copyright 2019 by SEAL Analytical. Not to be reproduced without permission.

9.4 WORKING STANDARDS

mL of Stock C µg/L as CN mL of Stock C µg/L as CN

Copyright 2019 by SEAL Analytical. Not to be reproduced without permission.

10 SAMPLE PRESERVATION AND STORAGE

According to ISO EN DIN 14403-2:2012:

1. Switch on all modules

Copyright 2019 by SEAL Analytical. Not to be reproduced without permission.

1. Switch the reagent lines:

14 SYSTEM CLEANING PROCEDURE

WEEKLY – SPECIAL CLEANING FOR THE DISTILLATION COIL:

Copyright 2019 by SEAL Analytical. Not to be reproduced without permission.

1. The distillation heating bath should have a temperature of 125 ± 1°C.

Copyright 2019 by SEAL Analytical. Not to be reproduced without permission.

16.1 TEST CONDITIONS

Sampler type XY3 sampler

16.2 PERFORMANCE DATA – OVERVIEW

Low range High range

Coefficient of Variation 0.10 µg/L, 0.4% 2.95 µg/L, 0.6%

Pooled SD 0.75 µg/L

Copyright 2019 by SEAL Analytical. Not to be reproduced without permission.

16.3 CALIBRATION DATA