Single use bioreactor series

For pluripotent stem cell stirred suspension culture

Made in Japan

100mL・30mL
EB formation
in
stirred suspension
culture

Cell expansion
Differentiation

PAT. pending

 Low share stress agitation

 Aggregates formation by gentle mixing
 Plastic, gamma radiation sterilized

6ch magnetic stirrer

For 100mL, 30mL

Very low heat diffusion

20-120rpm

This research is granted by the Japan Society for the Promotion of Science (JSPS) through the
"Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program)".
 Application
Viable cells Cell line ：253G1（human iPS cell）
Medium ：mTeSR1 (STEMCELL Technologies)
120
× 10000 cells/mL

100
Culture condition*
80
60 • Volume 100mL
• Agitation 40rpm
40
• Temp. 37℃
20 • pH 7.2
0 • DO 40% (Air saturation）
0 1 2 3 4 5 6
* with cell culture system “Bio Jr. 8” (ABLE)
Day

Reference
1: Matsuura K Fabrication of mouse embryonic stem cell-derived layered cardiac cell sheets using a
bioreactor culture system. PLoS One. 2012;7(12):e52176.
2: Matsuura K Creation of human cardiac cell sheets using pluripotent stem cells. Biochem Biophys Res
Commun. 2012 Aug 24;425(2):321-7.

Product information
Product name Cat. No.

Single use bioreactor for 100mL BWV-S10A

Single use bioreactor 30mL BWV-S03A

6ch magnetic stirrer BWS-S03N0S-6

Contact Us

日本語: http://www.able-biott.co.jp/
English: http://www.able-biott.co.jp/en/
Biott corporation Head office

6-10, Nishigoken-cho, Shinjuku-ku, Tokyo, 162-0812, Japan

Phone: +81-3-3260-0415
Fax : +81-3-3260-0407