Universidad ECCI

Inorganic impurity
removal from waste oil
and wash-down water by
Acinetobacter johnsonii
Sarith Darianna Cardenas
Luisa Fernanda Tavera Garcia
 Objectives
As a first objective we
have the conditions of As objective number 2 we
the cultures in the have to control the density
1 synthetic medium so
that they all adjust to 7.2
2 spectrophotometrically,
measuring it so that it
before the sterilization gives us 600mn
that is carried out

As third objective Study As fourth objective

the removal of inorganic Investigate the primary
3 impurities in used oil and 4 cleaning mechanism in
wash water by A. the oil phase.
Introduction
Biotreatment of inorganic
In oil production, a large quantity of waste oil is
generated, with a high recovery value, in the impurities has attracted much
form of discharged crude oil, oil marl and wash attention and has provided
water, and generally recovered by the promising methods of high
gravitational sedimentation of inorganic efficiency and low cost.
impurities at 353 K (in winter) or 363 K (in
summer)
 Methodology
Cell density was monitored spectrophotometrically by measuring optical
density at 600 nm. The inorganic impurity in the oil was determined by the
standard method of petroleum products and additives, determination of
mechanical impurities, gravimetric method (GB / T 511-2010). In the tests,
waste oil and wash water samples were taken from the recovery pool at
different periods. The impurity removal rate in the oil phase was calculated as
follows:
 results

Effects of different volumes of inoculum Effect of different aeration time intervals

on the elimination of inorganic impurities in A. johnsonii to eliminate inorganic
in the used oil and the wash water. impurities in used oil and wash water.
 Effect of different time intervals on spontaneous
sedimentation of inorganic impurity from oil–water
system.
As shown in Table 1, the good removal effect in Sample 1 occurred at 4 h
spontaneous sedimentation, with the turbidity of 66 NTU, while it took 6 h to obtain
the similar effect in Sample 2. Obviously, the transport of impurities from oil into
water signifi- cantly impeded the decrease ofturbidity in water. On the one hand, in
Sample 1 special sedimentation process of suspended solids coagulated by cells and
intermediate metabolites occurred tran-siently withoutthe disturbance of
wettability reversal ofimpurities in oil phase.
As shown in Table 2, inorganic impurities in all samples were efficiently removed from
waste oil and wash-down water, with the final absolute concentration in oil of 0.32%,
0.42% and 0.44%, respectively. The turbidity and sulfide in water were below 90 NTU
and 0.65 mg/L, respectively. Moreover, waste oils from different processes and
wash-down water from Recovery Pool were applied in the same experiments, and all of
them were efficiently cleansed with impurity, turbidity and sulfides below 0.5%, 100
NTU and 1 mg/L, respectively.
 Conclusions
Biotreatment of inorganic impurities that seriously hampered the recovery
of used oil was shown to be a promising platform for cleaning of used oil
and wash water. The process developed did not depend on aeration but on
biological effects, during which the inorganic impurities of the oil were
invested in an aqueous phase and the small particles coagulated in the
large porA aggregates. johnsonii, and then followed by gravity
sedimentation.