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Aspartate Aminotransferase Assay Reagent Kit AST) User's Manual V1.1e
Aspartate Aminotransferase Assay Reagent Kit AST) User's Manual V1.1e
Sample 0.2mL
Aspartate Aminotransferase Assay Test Method for Single Reagent:
Blank QC Sample
Reagent Kit (Aspartate Method) Working Reagent (mL) 3.0 3.0 3.0
Incubate to 37℃
[Product Name] Water (mL) 0.2
Aspartate Aminotransferase Assay Reagent Kit (Aspartate Method) QC Serum (mL) 0.2
Package Sizes]
Sample (mL) 0.2
Type Specification
Mix well, incubate at 37℃ for 60 seconds, and read absorbance A1. Read
R1: 25ml×4, R2: 25ml×2;
Common Types absorbance A2 after the next 60 seconds.
R1: 60ml×3, R2: 30ml×3. Test Method for Dual Reagent:
R1: 40ml×3, R2: 18ml×3;
Blank QC Sample
R1: 40ml×3, R2: 20ml×3;
Special-purposed Types R1 (mL) 2.0 2.0 2.0
R1: 50ml×3, R2: 25ml×3;
Incubate to 37℃
R1: 30ml×3, R2: 15ml×3. Water (mL) 0.2
[Intended Use]
QC Serum (mL) 0.2
In vitro test for the quantitative determination of AST concentration in Sample (mL) 0.20
serum and plasma. Incubate at 37℃ for 60-300 sec.
[Principle] R2 (mL) 1.0 1.0 1.0
Based on the method recommended by the Aspartate, the reaction principle Mix well, incubate at 37℃ for 60 seconds, and read absorbance A1. Read
is: absorbance A2 after the next 60 seconds. ΔA/min =(A1 - A2)/min.
L - Aspartate + a - Oxoglutarate ¾¾
AST
¾® Oxaloacetate + L - glutamate Calculation:
Oxaloacetate + NADH + H ¾¾¾
+ MDH
® L - malate + NAD + + H 2O
Interference by intrinsic pyruvate in the sample is eliminated in the presence
of lactic dehydrogenase.
TV = Total Volume of Reaction (mL);
Pyruvat + NADH + H + ¾¾¾ ¾¾¾
Lactic dehydrogenase
® L - lactate+ NAD + + H 2O SV = Sample Volume (mL);
[Reagents composition] 6.22 = Mmol extinction coefficient of NADH at 340nm;
Reagent 1 (R1) and Reagent 2 (R2). P = Optical Path (cm);
R1: Tris (concentration: 100mmol/L), NADH (concentration: 0.26mmol/L), Theoretical Factor K=2572.
EDTA (concentration: 3.5mmol/L), lactic dehydrogenase (concentration: [Reference Values]
3.0KU/L); R2: Tris (concentration: 100mmol/L), L-aspartic acid 37℃ ≤40U/L.
(concentration: 240mmol/L), α-oxoglutarate (concentration: 12mmol/L), Due to the normal and reasonable difference between regions and
MDH (concentration: 1.0KU/L). individuals as well as the different test methods adopted, the values of AST
Note:Do not exchange or mix the components of reagents of different lot concentration may vary. It is suggested that each laboratory establish its own
numbers. reference values for the population that it serves.
[Storage and stability] [Interpretation of Test Results]
Keep in dark place at 2-8℃. Do not freeze the reagents! Unopened reagents Aspartate aminotransferase (AST) level is high in myocardial cells. In the
are stable for 12 months. Opened reagents are stable for 1 month. case of myocardial infarction, the AST activity in serum increases, and
[Instruments] increases significantly 6~12h after attack, reaches the peak after 48h, and
This Reagent is mainly applicable to the following types of semi-automated returns to normal after about 3~5 days. AST in serum can also be originated
or automated biochemical analyzers. It is suggested that you verify test from liver cells. The various hepatic diseases may cause increase of AST in
results according to the actual situation of the laboratory when using this serum, sometimes up to 1200U or higher in the case of toxic hepatitis.
Reagent. Relative parameters available for professional user on request. Hepatitis, pleurisy, nephritis, pneumonia, etc. may also lead to slight
The list of instruments is as follows: increase of AST in serum.
Chemray 120, Chemray 160, Chemray 180, Chemray 200, Chemray 240, [Limitation of the Procedure]
Chemray 260, Chemray 280, Chemray 310, Chemray 420, Chemray 400, Sample hemolysis should be avoided. No interference was observed by
Chemray 380, Chemray 360, Chemray 320, Chemray 300, Chemray 800, bilirubin no more than 256.4µmol/L, triglyceride no more than 8mmol/L
Chemray 820, Chemray 850, Chemray 880, RT-1904C, RT-9000, RT-9100, and hemoglobin no more than 1g/L in the sample. When the AST activity in
RT-9200, RT-9300, RT-9500, RT-9600, RT-9800, RT-9900. the sample is greater than 550U/L, dilute with physiological saline. Multiply
[Specimen] the result by the dilution ratio.
Serum, heparin or EDTA plasma, Avoid hemolysis! AST is stable for 7 days [Performance Characteristics]
at 4℃. 1. Linear Range: 5U/L~550U/L(37℃). The correlation coefficient (r)
[Test Procedure] should be ≥ 0.990.
The reagents are ready for use. R1 and R2 can be mixed at the ratio of 2:1 2. Within-run Precision: CV ≤ 5%.
into a single reagent for use. The mixed single reagent is stable for 14 days 3. Between-run Precision: ≤ 10%.
at 2-8℃. 4. Accuracy: Relative Deviation ≤ ±10%.
Test Conditions: [Notes]
Temperature 37℃ Optical Path 1.0cm 1. The product is only for in vitro diagnosis and operated by medical
professionals or trained workers.
Wavelength 340nm Delay Time 60s
2. The dosage of reagents and samples may be changed proportionally
Reagent 3.0mL Test Time 60-180s
V1.1e
Rayto Life and Analytical Sciences Co., Ltd.
Temperature limit
Biological risks
Manufacturer
CE Mark
Use-by date
Caution!
Catalogue number
R017MMAY2017V110e
V1.1e