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and WB1 and OB1 had the highest TVPBC which, however, was lower than the CT. All the treated
products recorded a progressive increase in bacterial counts along with the increased duration of
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storage. The counts for colititre ( MPN) revealed their absence irrespective of the storage days and
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TABLE 1: Formulation for pork patties enriched with Wheat Bran fiber
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TABLE 2: Formulation for pork patties enriched with Oat Bran fiber
Ingredients (g/kg) Control(CT) Low fat pork patties
OB1 OB2 OB3
Lean meat 695 598.42 535.13 468.84
Added fat 150 50 50 50
Added water 50 120 120 120
Refined flour 35 35 35 35
Condiments 30 30 30 30
Spice mix 15 15 15 15
Salt 20 20 20 20
Sugar 2.0 2.0 2.0 2.0
Sodium tri polyphosphate 3.0 3.0 3.0 3.0
Sodium nitrite 120ppm 120ppm 120ppm 120ppm
Oat bran* - 126.58 189.87 256.16
*The total dietary fiber content of oatt bran (Beggry’s India limited, New Delhi) is 15.8 percent. Hence the dietary fiber content
of OB1, OB2 and OB3 formulations are 2 , 3 and 4 percent respectively.
Vol. 33, No. 2, 2014 121
physico chemical and microbiological qualities at The TVPBC of pork patties prepared by
four different storage periods. incorporation of increasing levels of WB and OB
The pH of pork patties was determined as differed significantly ( P< 0.01). Besides, TVPBC
per the method of Pippen et.al. ( 1965) while the showed a significant (P< 0.01) increasing trend from
water activity ( aw) was determined by following the 1 to 15 day of storage. The relatively low bacterial
st th
method of Labuza et. al ( 1976). The total viable counts recorded for the treated products as compared
psychotropic bacterial count ( TVPBC) was to the CT ones might be due to the incorporation of
determined as per the procedure laid down by APHA decreasing levels of fat and increasing levels of
(1966) and the colititre values were recorded dietary fiber, resulting in reduced risk of microbial
following the multiple tube technique as described contamination. However, recording of increased
by Harrigan and Mc Cancy ( 1976). The data TVPBC during storage might be due to the growth
obtained were analysed as per the method by SAS and multiplication of contaminating bacteria during
Enterprise guide (2008). the products preparation , processing, handling and
packaging. The findings of the present study
RESULTS AND DISCUSSION corroborated well with reports of Modi et.al. ( 2009)
The pH values of pork patties showed a slight and Kumar and Sharma( 2004) who also carried
increasing trend in both the treated formulations while out TVPBC in low fat mutton kofta and ground pork
the CT product recorded lowest pH on day -1 . This patties respectively.
slow increase in the pH values of treated products
The coli titre values (MPN) recorded for both
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reported by Yilmaz ( 2005). A decrease in the pH storage even upto 15 day were found to be nil. The
th
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values of meat balls prepared by incorporation of findings of the present study is in agreement with
OB and other non meat ingredients was also the reports of Kumar and Sharma ( 2004) who also
recorded by Yilmaz and Daglioglu ( 2003). Studies reported absence of coliform in low fat ground pork
on the effect of storage periods revealed gradual but patties during the seven days storage period.
significant ( P< 0.01) rise in the pH values of meat The different sensory qualities of pork patties
patties from day-1 to day 15th. This might be related i.e. the colour, flavour, juiciness and tenderness
to the accumulation of various metabolites and other scores decreased significantly (P < 0.01) along with
end products resulting from bacterial growth and increase in the levels of WB and OB and also on
multiplication ( Deva and Narayanan , 1988) increased duration of storage in the treated
besides, degradation /oxidation of fat during the formulations as compared to the CT product. The
storage days. overall acceptability scores of revealed that fiber
The mean aw values decreased significantly enriched pork patties registered progressively lower
(P< 0.01) in treated formulations as compared to scores along with the increasing levels of fiber in the
the CT and it was lowest in WB3 and OB 3 formulated products and the periods of storage Amongst the
pork patties. This progressive decrease in aw of the product formulations; WB1 and OB1 registered
treated products mi ght be ascri bed to the highest overall acceptability scores(6.58 to 7.6 and
incorporation of increased levels of WB and OB in 6.49 to 7.85) and WB3 and OB3 recorded lowest
the treated formulations. Further, aw of pork patties acceptability scores (6.53 to 7.77 and 6.40 to 7.68)
reduced markedly (P< 0.01) from 1 to 15th day of . The results were in accordance with the ones
storage, which might be due to the utilisation of
obtained for meat balls with added cereal bran (
available water by the microbes for their growth and
multiplication as reported by Dharmaveer et.al. Yasarlar et. al, 2007)and for chicken patties with
( 2007).As in the present study, gradual decrease in added wheat and oat bran ( Talukdar and Sharma
aw of the meat products stored under refrigeration ,2010.). Further, the overall acceptability scores
was also observed by also observed viz. Rajkumar decreased significantly (P < 0.01) on increased days
and Berwal (2004) in chevon patties, Dharmaveer of storage i.e. from 1st to 15th. This might primarily
et.al. ( 2007) in smoked chevon sausages and be due to the reduction of flavour and colour scores
Andres et. al. (2006) in low fat chicken sausages. consequence to the development of some volatile
122 ASIAN JOURNAL OF DAIRY & FOOD RESEARCH
flavour component due to the oxidation of fat along with the increase in storage period and
besides, other associated factors i.e. decreased irrespective of the product formulations. The water
moisture content, increased pH values, production activity (aw ) values of the treated products were
of bacterial metabolites etc. Though significant significantly lower as compared to CT ones, during
differences in respect of sensory qualities were the storage period from 1st to 15th day. Amongst the
observed among different patty formulations, all were fiber enriched products, WB3 and OB3 revealed
within acceptable limit. Hence, the study further lowest while WB1 and OB1 recorded the highest
revealed that low fat pork patties with satisfactory TVPBC. Progressive increase in bacterial counts
eating qualities could be prepared by incorporation along with the increased storage periods was
observed in all the treated products. The colititre (
of WB and OB upto 4% level and can safely be
MPN) count was found to be zero, irrespective of
stored under refrigeration upto 15 days. the storage days both in CT and treated products.
CONCLUSION The study also revealed that low fat pork patties with
The pH of pork patties did not differ satisfactory eating qualities could be prepared by
significantly between the CT and treated products. incorporation of WB and OB upto 4% level and can
However, an increasing trend in pH was observed safely be stored under refrigeration upto 15 days.
REFERENCES
American Public Health Association. (1966). Standard Methods for Examining the Dairy Products. XI New York.
Andres, S. C., Garcia, M. E., Zaritzky, N. E. and Califano, A. N. (2006). Storage stability of low-fat chicken sausages.
Downloaded From IP - 202.141.75.49 on dated 20-Apr-2016
Dharmaveer, S., Rajkumar, V. and Mukesh, K. P. (2007). Quality and shelf life of smoked chevon sausages packed under
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