363

ProStar 363 Fluorescence Detector

Increased Sensitivity for All Fluorescence Applications

• Continuous Xenon lamp maximizes

sensitivity
• Time-programmable wavelength
changes optimize sensitivity
• Fully integrated with Star
Workstation software

Advantages of Fluorescence Spectroscopy Total Control Over Wavelength Selection

Fluorescence spectroscopy enjoys two distinct benefits The dual monochromator design of the ProStar 363
over UV-Vis spectroscopy–increased sensitivity and ensures the ability to select wavelengths specific to the
enhanced selectivity. Sensitivity is dramatically increased compounds of interest. No cut-off filters are needed for
by looking for a fluorescence signal against a dark multiple sample analysis. Time programming wavelength
background. Selectivity is achieved by selecting both the changes assures that each peak’s intensity is maximized.
excitation and emission wavelengths to screen out
unwanted interferences. Varian’s ProStar 363 Fluorescence
Detector maximizes sensitivity and selectivity to give you
total control over your analyses.

Sensitivity is the Key

The ProStar 363 provides the highest sensitivity by using
a continuous Xenon lamp. Continuous lamps generate a
significantly more stable signal than flashlamps.
Consequently, drift and noise are dramatically reduced.
The unique optical design of the ProStar 363 permits
simultaneous measurement of both emission and Chromatogram of polyaromatic hydrocarbons using time
programmed wavelength changes to optimize the signal intensity
excitation signals, thus accurately correcting for lamp for each compound (red arrows indicate wavelength change).
fluctuations. The combination of the lamp and optics
provide the quietest signal possible, resulting in more Scan Both Excitation and Emission Spectra
reproducible fluorescent measurements. The ProStar 363 is fully capable of scanning both the
excitation and emission spectra. The peak excitation and
Xenon Lamp emission wavelength information can then be used for
Excitation Grating
time programming wavelength changes to ensure
Excitation Slit
Excitation Window maximum sensitivity for each compound.
Reference Diode

Transmission Window

Flowcell
Emission Window Easy System Control
Emission Slit
Program the detector either through the easy-to-use front
Photomultiplier panel or via Star Workstation software. Run progress can
Tube (PMT)
be viewed either on the front display or Star Workstation.
Emission Grating Built-in GLP functions include wavelength calibration
Optics Diagram of the ProStar 363 Detector and a lamp hour counter.
ProStar 363 Fluorescence Detector

Technical Data
Optical design: Dual monochromators
Sensitivity: S/N 350:1, Raman line of water
Wavelength range: 200-731 nm standard; 200-900 nm with optional red-sensitive PMT
Wavelength accuracy: ± 3 nm
Wavelength reproducibility: ± 0.5 nm
Spectral bandwidth: 15 nm for excitation, user selectable 15 or 30 nm for emission
Light source: 150W continuous Xenon lamp
Flow cell: Quartz, PTFE, 12 µL illuminated volume
Analog outputs: 2, use for output to fraction collector or data systems
Display: 2 x 24 character, high contrast LCD
Spectra storage: Up to 8 spectra, either excitation or emission can be added to or subtracted from each other
Dimensions: 29.2 cm (w) x 30.5 cm (h) x 47.0 cm (d)
11.5 in. (w) x 12.0 in. (h) x 18.5 in. (d)
Weight: 19 kg (42 lb)

Operation within specifications from 10 °C to 40 °C. Operates in relative humidities from 5% to 95%, non-condensing.

Ordering Information
393572801 ProStar 363 Fluorescence Detector
393572802 ProStar 363 Fluorescence Detector with optional ADC computer card
5618000200 Extended wavelength option (200-900 nm)

Varian, Inc. • www.varianinc.com

• North America: 800.926.3000, 925.939.2400
• Europe The Netherlands: 31.118.67.1000
• Asia Pacific Australia: 613.9560.7133
• Latin America Brazil: 55.11.3845.0444
• Other sales offices and dealers throughout the world

GC • GC/MS • HPLC • AAS • ICP • ICP-MS • UV-Vis-NIR • Fluorescence • Dissolution • NMR • Analytical Supplies

WCI-pdf-0004 10/01 U.S.A.