Number: TB.199648.

01 Technology Platform: Pathogens

Effective Date: April 24, 2014 Originating Location: St. Paul, MN
Supersedes: (New)

3MTM PetrifilmTM Salmonella Express System

Performance Summary - Inclusivity and Exclusivity
The 3M™ Petrifilm™ Salmonella Express System is used for the rapid qualitative detection and biochemical confirmation of
Salmonella in enriched food and food process environmental samples. The 3M Petrifilm Salmonella Express System consists
of the 3M™ Petrifilm™ Salmonella Express Plate, 3M™ Petrifilm™ Salmonella Express Confirmation Disk, 3M™ Salmonella
Enrichment Base, and the 3M™ Salmonella Enrichment Supplement. For high microbial bioburden samples only, a
secondary enrichment of Rappaport-Vassiliadis R10 (R-V R10) is used.

The 3M Petrifilm Salmonella Express Plate is a sample ready-to-use chromogenic culture medium system that contains a
cold-water-soluble gelling agent and is selective and differential for Salmonella, providing a presumptive result. The 3M
Petrifilm Salmonella Express Confirmation Disk contains a biochemical substrate that facilitates the biochemical confirmation
of Salmonella organisms. The 3M Salmonella Enrichment Broth is used with the 3M Salmonella Enrichment Supplement for
the selective enrichment of Salmonella species in foods and food process environmental samples in conjunction with the 3M
Petrifilm Salmonella Express Plate and 3M Petrifilm Salmonella Express Confirmation Disk.

Method Selectivity – Inclusivity and Exclusivity Testing

Method selectivity testing was performed by 3M Food Safety.

Study Design:

Pure bacterial cultures were derived from purchased lyophilized preparations or from frozen stock cultures.

There were two procedures performed for the Inclusivity and Exclusivity Study.

1. Procedure A –Low Microbial Bioburden Method:

To represent low microbial bioburden sample testing, Salmonella strains were diluted and inoculated into prepared 3M
Salmonella Enrichment Base with 3M Salmonella Enrichment Supplement, and incubated at 41.5°C for 18 hours.

After enrichment incubation, samples were individually streaked onto hydrated 3M Petrifilm Salmonella Express Plates.
Plates were incubated at 41.5°C for 22-26 hours.

After plate incubation, presumptive positive colonies were circled and 3M Petrifilm Salmonella Express Confirmation Disks
were applied. Disked plates were incubated at 41.5°C for 4-5 hours.

2. Procedure B – High Microbial Bioburden Method:

TB.199648.01 Page 1 of 6
3MTM PetrifilmTM Salmonella Express System – Performance Summary (cont.)
The Low Microbial Bioburden Method (Procedure A) was followed as above with the following exception: after the primary
enrichment, 0.1 mL of each primary enrichment sample was transferred into 10 mL of Rappaport-Vassiliadis R10 (R-V R10)
for secondary enrichment, and incubated at 41.5°C for 8 hours.

After secondary enrichment incubation, R-V R10 samples were individually streaked onto hydrated 3M Petrifilm Salmonella
Express Plates and plates were incubated at 41.5°C for 22-26 hours. After plate incubation, the presumptive positive colonies
were circled and 3M Petrifilm Salmonella Express Confirmation Disks were applied. Disked plates were incubated at 41.5°C
for 4-5 hours.

Results:

Inclusivity - false negative rate

Inclusivity is defined as the ability of a method to detect the target analyte from a wide range of strains. One hundred and five
(105) Salmonella isolates (Appendix 1) were tested. A false negative rate of < 1% (>99% inclusivity) was determined. Only
one strain of one serotype, Salmonella enterica enterica Gallinarum, was shown to be negative by the 3M method.

Exclusivity - false positive rate

Exclusivity is defined as the lack of interference from a relevant range of non- target strains. Thirty two (32) non - Salmonella
isolates (Appendix 2) were tested. A false positive rate of 0% (100% exclusivity) was determined.

The following two tables summarize the results of the Inclusivity and Exclusivity Studies as related to false negative and false
positive rates.

Table 1. Inclusivity - False Negative Rate Results Summary:

3M Petrifilm Salmonella 3M Petrifilm # False Negative Total # Strains False Negative Rate
Express System Method Salmonella Express Results Tested (%)
System components

Low Plate Only 1 105 <1

Low Plate + Disk 1 105 <1
High Plate Only 1 105 <1
High Plate + Disk 1 105 <1

Table 2. Exclusivity - False Positive Rate Results Summary:

3M Petrifilm Salmonella 3M Petrifilm # False Positive Total # Strains False Positive Rate
Express System Method Salmonella Express Results Tested (%)
System components

Low Plate Only 0 32 0

Low Plate + Disk 0 32 0
High Plate Only 0 32 0
High Plate + Disk 0 32 0

Food Safety
3M Center 3M is a trademark of 3M.
Building 275-5W-05 Used under license in Canada.
St. Paul, MN 55144-1000 Please recycle. Printed in U.S.A.
USA © 3M 2014. All rights reserved.
1-800-328-6553 70-2011-5063-1
www.3M.com/foodsafety
TB.199648.01 Page 2 of 6
3MTM PetrifilmTM Salmonella Express System – Performance Summary (cont.)

Appendix 1 – Salmonella inclusivity test strains:

Genus, species, subspecies Serotype / Group ID

Salmonella enterica enterica Abaetetuba TICC 2736
Salmonella enterica enterica Aberdeen TICC 2500
Salmonella enterica enterica Abony NCTC 6017
Salmonella enterica enterica Abortusequi ATCC 9842
Salmonella enterica enterica Adelaide TICC 2501
SGSC 2458, electrophoretic type Ag1;
Salmonella enterica enterica Agona
SARB1
Salmonella enterica enterica Amsterdam TICC 2660
Salmonella enterica enterica Anatum TICC 2668
Salmonella enterica enterica Augustenborg TICC 2670
Salmonella enterica enterica Bareilly ATCC 9115
Salmonella enterica enterica Berta ATCC 8392
Salmonella enterica enterica Birkenhead TICC 1273
Salmonella enterica enterica Blockley ATCC 51961
Salmonella enterica enterica Bovis-morbificans TICC 1293
Salmonella enterica enterica Braenderup TICC 2508
Salmonella enterica enterica Brandenburg TICC 2674
Salmonella enterica enterica Brazil NCTC 8446
Salmonella enterica enterica Bredeney TICC 2510
Salmonella enterica enterica Cerro TICC 2176
Salmonella enterica enterica Choleraesuis ATCC 1070
Salmonella enterica enterica Derby TICC 2519
Salmonella enterica enterica Dublin TICC 2757
Salmonella enterica enterica Emek TICC 1447
Salmonella enterica enterica Enteritidis ATCC 13076
Salmonella enterica enterica Gallinarum TICC 1320
Salmonella enterica enterica Gaminara ATCC 8324
Salmonella enterica enterica Give TICC 1282
Salmonella enterica enterica Hadar TICC 2528
Salmonella enterica enterica Havana TICC 443
Salmonella enterica enterica Heidelberg TICC 561
Salmonella enterica enterica Hessarek TICC 2655
Salmonella enterica enterica Indiana ATCC 51959
Salmonella enterica enterica Infantis TICC 2616
Salmonella enterica enterica Johannesburg TICC 2543
Salmonella enterica enterica Kahla ATCC 17980
Salmonella enterica enterica Kedougou TICC 4102
Salmonella enterica enterica Kentucky TICC 2620

Food Safety
3M Center 3M is a trademark of 3M.
Building 275-5W-05 Used under license in Canada.
St. Paul, MN 55144-1000 Please recycle. Printed in U.S.A.
USA © 3M 2014. All rights reserved.
1-800-328-6553 70-2011-5063-1
www.3M.com/foodsafety
TB.199648.01 Page 3 of 6
3MTM PetrifilmTM Salmonella Express System – Performance Summary (cont.)

Appendix 1 (cont.) – Salmonella inclusivity test strains:

Genus, species, subspecies Serotype / Group ID

Salmonella enterica enterica Kiambu TICC 2692
Salmonella enterica enterica Kimberley TICC 778
Salmonella enterica enterica Kirkee 3M 1485
Salmonella enterica enterica Kokomlemle TICC 2544
Salmonella enterica enterica Kottbus TICC 2621
Salmonella enterica enterica Lansing TICC 2622
Salmonella enterica enterica Lexington TICC 2624
Salmonella enterica enterica Lille TICC 2684
Salmonella enterica enterica Litchfield TICC 2547
Salmonella enterica enterica Livingstone TICC 2549
Salmonella enterica enterica Llandoff TICC 1291
Salmonella enterica enterica London TICC 2550
Salmonella enterica enterica Manhattan TICC 2551
Salmonella enterica enterica Mbandaka TICC 1289
Salmonella enterica enterica Miami SGSC 2485
Salmonella enterica enterica Montevideo TICC 763
SGSC 2490, electrophoretic type Mu2;
Salmonella enterica enterica Muenchen
SARB33
Salmonella enterica enterica Naestved SGSC 3612, 88/8
Salmonella enterica enterica Newport ATCC 6962
Salmonella enterica enterica Nottingham NCTC 7832
Salmonella enterica enterica Ohio TICC 444
Salmonella enterica enterica Oranienburg TICC 2560
Salmonella enterica enterica Orion TICC 720
SGSC 2496, electrophoretic type Pn1;
Salmonella enterica enterica Panama
SARB39
SGSC 2499, electrophoretic type Pa1;
Salmonella enterica enterica Paratyphi A
SARB42
Salmonella enterica enterica Paratyphi B TICC 2240
Salmonella enterica enterica Paratyphi C SGSC 3592, 4034-88
Salmonella enterica enterica Poona TICC 2472
Salmonella enterica enterica Reading SGSC 2510
Salmonella enterica enterica Rissen TICC 2644
Salmonella enterica enterica Rubislaw TICC 2645
Salmonella enterica enterica Saint Paul TICC 559
Salmonella enterica enterica Schwarzengrund TICC 4513
SGSC 2515, electrophoretic type Se1;
Salmonella enterica enterica Sendai
SARB58
Salmonella enterica enterica Senftenberg TICC 2178

Food Safety
3M Center 3M is a trademark of 3M.
Building 275-5W-05 Used under license in Canada.
St. Paul, MN 55144-1000 Please recycle. Printed in U.S.A.
USA © 3M 2014. All rights reserved.
1-800-328-6553 70-2011-5063-1
www.3M.com/foodsafety
TB.199648.01 Page 4 of 6
3MTM PetrifilmTM Salmonella Express System – Performance Summary (cont.)
Appendix 1 (cont.) – Salmonella inclusivity test strains:

Genus, species, subspecies Serotype / Group ID

Salmonella enterica enterica Singapore TICC 2652
SGSC 2517, electrophoretic type St1;
Salmonella enterica enterica Stanley
Biotype 26bei.; SARB60
Salmonella enterica enterica Tennessee TICC 2577
SGSC 2519, electrophoretic type Th1;
Salmonella enterica enterica Thompson
SARB62
Salmonella enterica enterica Typhi ATCC 9993
Salmonella enterica enterica Typhimurium ATCC 14028
Salmonella enterica enterica Typhimurium (nm) 3M 1447
Salmonella enterica enterica Typhimurium (nm) 3M 1448
Salmonella enterica enterica Typhimurium (nm) 3M 1449
Salmonella enterica enterica UNNAMED SGSC 3038
Salmonella enterica enterica Virchow TICC 552
Salmonella enterica enterica Virginia TICC 2659
Salmonella enterica enterica Wandsworth TICC 2661
Salmonella enterica enterica Waycross TICC 566
Salmonella enterica enterica Welikade TICC 2586
Salmonella enterica enterica Weltevreden TICC 2663
Salmonella enterica enterica Westhampton TICC 3053
SGSC 2528, electrophoretic type Wi1;
Salmonella enterica enterica Wien
SARB71
Salmonella enterica enterica Worthington TICC 2587
Salmonella enterica enterica Zanzibar TICC 2589
Salmonella enterica enterica Zehlendorf TICC 2395
Salmonella enterica houtenae (IV Argentina) SGSC 2555
Salmonella enterica houtenae (IV Wassenaar) SGSC 2576
Salmonella enterica houtenae UNNAMED SGSC 3084
Salmonella enterica houtenae UNNAMED NCTC 10401
Salmonella enterica indica (VI Flint) SGSC 2554
Salmonella enterica indica (VI Vrinkaban) SGSC 2582
Salmonella enterica indica UNNAMED SGSC 3116
Salmonella enterica indica (India) ATCC BAA-1578
Salmonella enterica salamae UNNAMED ATCC 6959
Salmonella enterica salamae (Betioky) ATCC 700152
Salmonella enterica salamae (Tranoroa) ATCC 700148
Salmonella enterica salamae (II Setubal) SGSC 2567

Culture Strain Source and Abbreviations: American Type Culture Collection (ATCC - USA); National Collection of Type Cultures (NCTC -
UK); 3M Tecra Culture Collection (TICC – Australia); Pennsylvania State University Department of Veterinary and Biomedical Sciences
(PSU – USA); and/or University of Calgary Salmonella Genetic Stock Center (SGSC – Canada)

Food Safety
3M Center 3M is a trademark of 3M.
Building 275-5W-05 Used under license in Canada.
St. Paul, MN 55144-1000 Please recycle. Printed in U.S.A.
USA © 3M 2014. All rights reserved.
1-800-328-6553 70-2011-5063-1
www.3M.com/foodsafety
TB.199648.01 Page 5 of 6
3MTM PetrifilmTM Salmonella Express System – Performance Summary (cont.)

Appendix 2 – Non-Salmonella exclusivity test strains:

Genus, species, subspecies Serotype / Group ID

Cedecea neteri ATCC 33855
Citrobacter brakii ATCC 29063
Citrobacter diversus TICC 1604
Citrobacter freundii ATCC 8090
Citrobacter koseri TICC 4469
Citrobacter sedlakii TICC 4088
Citrobacter youngae TICC 4255
Cronobacter sakazakii ATCC 29544
Edwardsiella tarda NCTC 10396
Enterobacter aerogenes ATCC 13048
Enterobacter amnigenus ATCC 51816
Enterococcus faecalis ATCC 14506
Escherichia coli ATCC 25922
Escherichia coli O157:H7 PSU 93.0134
Escherichia hermanii TICC 2489
Escherichia vulneris TICC 447
Hafnia alvei ATCC 51815
Klebsiella oxytoca ATCC 51817
Klebsiella pneumoniae ATCC 23357
Morganella morganii ATCC 25830
Pantoea spp TICC 4488
Proteus mirabilis ATCC 43071
Proteus vulgaris ATCC 13315
Providencia rettgeri TICC 4472
Serratia ficaria TICC 4475
Serratia liquefaciens ATCC 51814
Serratia marcesens ATCC 14041
Shigella sonnei ATCC 25931
Staphylococcus aureus ATCC 25923
Yersinia enterocolitica ATCC 23715
Yersinia kristensenii ATCC 33639
Yersinia ruckerii ATCC 29473

Culture Strain Source and Abbreviations: American Type Culture Collection (ATCC - USA); National Collection of Type Cultures (NCTC -
UK); 3M Tecra Culture Collection (TICC – Australia); Pennsylvania State University Department of Veterinary and Biomedical Sciences
(PSU – USA); and/or University of Calgary Salmonella Genetic Stock Center (SGSC – Canada)

Food Safety
3M Center 3M is a trademark of 3M.
Building 275-5W-05 Used under license in Canada.
St. Paul, MN 55144-1000 Please recycle. Printed in U.S.A.
USA © 3M 2014. All rights reserved.
1-800-328-6553 70-2011-5063-1
www.3M.com/foodsafety
TB.199648.01 Page 6 of 6