Visium Document Resources

1 Experimental Design Visium Spatial Protocol Planner Imaging Guidelines

& Planning
Planner Technical Note
Before starting the CG000295 CG000241
experiment, refer to
these documents for Visium Spatial Slide Reset
protocol planning,
imaging guidelines and Demonstrated Protocol
slide reset instructions CG000332

2 Sample Preparation Tissue Preparation Guide

Tips and best
Demonstrated Protocol Watch
practices for preparing
CG000240 Video
a tissue block and
placing sections on
Visium slides.

3 Tissue Optimization Methanol Fixation Methanol Fixation

Determine tissue + H&E Staining + Immunofluorescence
Demonstrated Protocol Watch Demonstrated Protocol
compatibility
CG000160 Video CG000312
and identify the
optimal tissue Visium Spatial Tissue
permeabilization time Optimization
for each tissue type. User Guide Watch
CG000238 Video

4 Library Construction Methanol Fixation Methanol Fixation

After the optimal + H&E Staining + Immunofluorescence
Demonstrated Protocol Watch Demonstrated Protocol
permeabilization
CG000160 Video CG000312
time has been
identified, perform
Visium Spatial Gene Sequencing Metrics
library construction & Base Composition
Expression
and spatial gene User Guide Watch Technical Note
expression mapping. CG000239 Video CG000290

5 Targeting (optional)
Targeted Gene Expression
Target relevant genes - Spatial
in Visium Spatial Gene User Guide
Expression libraries CG000377
with Targeted Gene
Expression.

10xGenomics.com
Visium Document Resources