FACULTY OF INDUSTRIAL SCIENCES AND TECHNOLOGY

BSB2452 ENZYME TECHNOLOGY LABORATORY

SEMESTER II 2021/2022

PRACTICAL 6

IMMOBILIZATION OF ENZYME/PROTEIN ON SOLID SURFACE

SECTION 01

NAME MATRIC ID Part of contribution

NUR LIYANA BINTI ISMAIL SB20091 METHODOLOGY

RESULT

NUR AISYAH BINTI ZULKIFLI SB20093

MUHAMMAD HAMZAH BIN MUSTAFFA KAMAL SB20056

AIDA SYAFINI BINTI MOHD AKHIR SB20012

PERSON IN CHARGE:

Marks Distribution:

Bil. Lab report section Mark Marks obtained

distribution
1 Introduction 10
2 Objective(s) 5
3 Materials and Methods 10
4 Results and Answers to questions 20
5 Discussion 40
6 Conclusion 5
7 References 5
8 Appendix 5
TOTAL
1.0 INTRODUCTION

2.0 OBJECTIVES
3.0 METHODOLOGY

Chemical and reagents

1. Enzyme/protein
2. PBS
3. Polymer (Chitosan)
4. Glass slide
5. Cuvette
6. Pipette
7. Syringe

Working process
1. Enzyme or protein solution was prepared by adding 1mg of enzyme or proteins into a 10
ml Phosphate Buffer Saline (PBS pH 7.0)

2. Polymer solution was prepared by mixing 100g of chitosan in 10ml of water with 2% of
acetic acid.

3. For blank, PBS solution was placed into cuvette.

4. For control, the mixture of enzyme with PBS only was placed into the cuvette and was
let to rest for 24 hours.

5. As for the test, the mixture of enzyme with PBS and chitosan was placed into the
cuvette and was let to rest 24 hours.

6. The samples absorbance reading was taken by UV-vis Spectra under 406nm wavelength
and was recorded.
4.0 RESULT
Sample Control Test Blank
OD406nm (A) 0.000 0.127 0.592

Table 1: The absorbance values of cuvettes at 406nm spectrophotometer

Absorbance reading, A vs Samples

0.7
Absorbance Reading, A

0.6

0.5

0.4

0.3

0.2

0.1

0
Blank Control Test

Samples

Graph 1: Absorbance reading, A vs Samples.

5.0 DISCUSSION
6.0 CONCLUSION
7.0 REFERENCES
8.0 APPENDIX