44-74567man Series200arefractiveindexdetector

LIQUID CHROMATOGRAPHY
SERIES 200A
REFRACTIVE INDEX DETECTOR
User’s Guide
Release History
Part Number Release Publication Date
9936751 B Nov. 2005
Any comments about the documentation for this product should be addressed to:
User Assistance
PerkinElmer LAS
710 Bridgeport Avenue
Shelton, Connecticut 06484-4794
U.S.A.
Or emailed to: info@perkinelmer.com
Notices
The information contained in this document is subject to change without notice.
Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no
warranty of any kind with regard to this document, including, but not limited to, the
implied warranties of merchantability and fitness for a particular purpose.
PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in
connection with furnishing, performance or use of this material.
Copyright Information
This document contains proprietary information that is protected by copyright.
All rights are reserved. No part of this publication may be reproduced in any form whatsoever or
translated into any language without the prior, written permission of PerkinElmer, Inc.
Copyright © 2005 PerkinElmer, Inc.
Produced in the USA
Trademarks
Registered names, trademarks, etc. used in this document, even when not specifically marked as such,
are protected by law.
PerkinElmer is a registered trademark of PerkinElmer, Inc.

e-ssentials is a trademark of PerkinElmer, Inc.
SERIES 200a User’s Guide. 3
Table of Contents
Introduction ......................................................................................5
Note...................................................................................................... 7
Warranty ............................................................................................... 8
About This Manual.................................................................................. 9
Conventions Used in this Manual............................................................ 10
Notes, cautions and warnings.......................................................... 10
Warning Signs on the Instrument........................................................... 13
Safety Practices ..............................................................................15
Overview ............................................................................................. 17
Precautions .......................................................................................... 18
General Operating Conditions ................................................................ 20
Environmental Conditions ...................................................................... 21
Electrical Safety.................................................................................... 22
WEEE Instructions for PerkinElmer Products ........................................... 23
Features and Specifications ............................................................25
Features .............................................................................................. 27
Specifications ....................................................................................... 28
Detection Principle..........................................................................31
Optical System ..................................................................................... 33
Flowcell Connections............................................................................. 35
Electronics Circuitry .............................................................................. 36
Signal processing circuit.................................................................. 37
Instrument Functions and Touch Screens ......................................39
Front Panel .......................................................................................... 41
Back Panel ........................................................................................... 43
Side Panel............................................................................................ 45
Display ................................................................................................ 46
HOME Screen (Indicators and On-Screen Buttons) ............................ 46
PARAMETERS Screen (Operating Parameters)................................... 48
PARAMETERS Screen (Start Up Sequence) ....................................... 49
Installation......................................................................................51
Overview ............................................................................................. 53
Power Connection and Grounding .......................................................... 54
Signal Cable Connections ...................................................................... 55
To connect the wire ....................................................................... 56
To release the wire ........................................................................ 56
Tube Connections................................................................................. 57
Operating Procedures ........................................................................... 58
[ Unattended Start Up ] .................................................................. 58
[ Manual Start ] ............................................................................. 59
《 Error Messages 》 ..................................................................... 61
Accessories .......................................................................................... 63
Spares Parts Available........................................................................... 64
Maintenance....................................................................................65
Overview ............................................................................................. 67
Flowcell Cleaning ........................................................................... 68
Replacing the Fuse......................................................................... 68
Cleaning of the Detector Exterior..................................................... 69
Validation ........................................................................................71
Span Validation .............................................................................. 74
Troubleshooting ..............................................................................75
Overview ............................................................................................. 77
Troubleshooting Charts ......................................................................... 78
Appendix .........................................................................................81
Miscibility Chart of Solvents ................................................................... 83
Index................................................................................................... 85
Introduction
6 . Series 200a User’s Guide
Note
Users of the Series 200a Refractive Index Detector are requested to use it in strict
accordance with the instructions in this User’s Guide.
Nothing contained in this User’s Guide will be construed as giving any guarantee on Series
200a Refractive Index Detector or granting or implying any license or immunity under any
patent or other rights.
The Series 200a Refractive Index Detector must be used on the user's own responsibility and
in strict compliance with all applicable laws and regulations.
The information contained in this User’s Guide was obtained from sources which we believe
are reliable, but no warranty or representation is hereby given as to its accuracy or
completeness.
This manual does not expect the Series 200a Refractive Index Detector to be used for clinical
or medical purposes.
The content of this User’s Guide is subject to change without notice.

Warranty
Except for a written warranty signed by its duly authorized representative and issued
particularly, PerkinElmer makes no warranty, express or implied, written or oral, statutory or
otherwise, as to the quality, performance, workmanship, fitness for a particular purpose, or
merchantability of the Series 200a.
Introduction . 9
About This Manual

This user’s guide is divided into following chapters:
Chapter 1 Introduction
This chapter contains a brief introduction on the instrument, the conventions and warnings
used in the manual.
Chapter 2 Safety Practices
Important safety information is provided in this chapter.
Chapter 3 Features and Specifications
This chapter contains features and specification of the Series 200a.
Chapter 4 Detection Principle
This chapter discusses the principles of detection for the Series 200a.
Chapter 5 Instrument Functions and Touch Screens
This chapter contains information on the components of the instrument, how it works and
information on the touch screens.
Chapter 6 Installation
Information on installing and re-installing your instrument should you ever need to move
your system is provided.
Chapter 7 Maintenance
Maintenance and cleaning procedures for the various components of your instrument are
provided.
Chapter 8 Validation
This chapter provides validation information.
Chapter 9 Troubleshooting
Troubleshooting information, software and system error messages are provided for the
instrument.
Appendix
The miscibility chart of solvents is in this appendix.

Conventions Used in this Manual

Normal text is used to provide information and instructions.
Bold text refers to text that is displayed on the screen.
UPPERCASE text, for example ENTER or ALT, refers to keys on the PC keyboard. '+' is used
to show that you have to press two keys at the same time, for example, ALT+F.
All eight digit numbers are PerkinElmer part numbers unless stated otherwise.
Notes, cautions and warnings

Three terms, in the following standard formats, are also used to highlight special
circumstances and warnings.
NOTE: A note indicates additional, significant information that is provided with some procedures.
Introduction . 11
We use the term CAUTION to inform you about situations that could
CAUTION
result in serious damage to the instrument or other equipment.
Details about these circumstances are in a box like this one.
D Caution (Achtung)
Bedeutet, daß die genannte Anleitung genau befolgt werden muß, um
einen Geräteschaden zu vermeiden.
DK Caution (Bemærk)
Dette betyder, at den nævnte vejledning skal overholdes nøje for at
undgå en beskadigelse af apparatet.
E Caution (Advertencia)
Utilizamos el término CAUTION (ADVERTENCIA) para advertir sobre
situaciones que pueden provocar averías graves en este equipo o en
otros. En recuadros éste se proporciona información sobre este tipo de
circunstancias.
F Caution (Attention)
Nous utilisons le terme CAUTION (ATTENTION) pour signaler les
situations susceptibles de provoquer de graves détériorations de
l'instrument ou d'autre matériel. Les détails sur ces circonstances
figurent dans un encadré semblable à celui-ci.
I Caution (Attenzione)
Con il termine CAUTION (ATTENZIONE) vengono segnalate situazioni
che potrebbero arrecare gravi danni allo strumento o ad altra
apparecchiatura. Troverete informazioni su tali circostanze in un riquadro
come questo.
NL Caution (Opgelet)
Betekent dat de genoemde handleiding nauwkeurig moet worden
opgevolgd, om beschadiging van het instrument te voorkomen.
P Caution (Atenção)
Significa que a instrução referida tem de ser respeitada para evitar a
danificação do aparelho.
We use the term WARNING to inform you about situations that could
result in personal injury to yourself or other persons. Details about
these circumstances are in a box like this one.
WARNING
D Warning (Warnung)
Bedeutet, daß es bei Nichtbeachten der genannten Anweisung zu
einer Verletzung des Benutzers kommen kann.
DK Warning (Advarsel)
Betyder, at brugeren kan blive kvæstet, hvis anvisningen ikke
overholdes.
E Warning (Peligro)
Utilizamos el término WARNING (PELIGRO) para informarle sobre
situaciones que pueden provocar daños personales a usted o a
otras personas. En los recuadros como éste se proporciona
información sobre este tipo de circunstancias.
F Warning (Danger)
Nous utilisons la formule WARNING (DANGER) pour avertir des
situations pouvant occasionner des dommages corporels à
l'utilisateur ou à d'autres personnes. Les détails sur ces circonstances
sont données dans un encadré semblable à celui-ci.
I Warning (Pericolo)
Con il termine WARNING (PERICOLO) vengono segnalate situazioni
che potrebbero provocare incidenti alle persone. Troverete
informazioni su tali circostanze in un riquadro come questo.
NL Warning (Waarschuwing)
Betekent dat, wanneer de genoemde aanwijzing niet in acht wordt
genomen, dit kan leiden tot verwondingen van de gebruiker.
P Warning (Aviso)
Significa que a não observância da instrução referida poderá causar
um ferimento ao usuário.
Introduction . 13
Warning Signs on the Instrument
WARNING
DANGEROUS VOLTAGES EXPOSED

WHEN COVER IS REMOVED
UNPLUG POWER CORD
WARNING
FOR CONTINUED PROTECTION

REPLACE ONLY WITH SAME FUSE
TYPE AND RATING
Safety Practices
Safety Practices . 17
Overview
This chapter describes the general safety practices and precautions that must be observed
when operating the Series 200a.
This advice is intended to supplement, not supersede, the normal safety codes in the user's
country. It is also a supplement to the PerkinElmer standard Safety and Health Policy. The
information provided does not cover every safety procedure that should be practiced.
Ultimately, maintenance of a safe laboratory environment is the responsibility of the analyst
and the analyst's organization.
Please consult all manuals supplied with the Series 200a and accessories before you start
working with the instrument. Carefully read the safety information in this chapter and in the
other manuals supplied. When setting up the instrument or performing analyses or
maintenance procedures, strictly follow the instructions provided.
Precautions
We use the term WARNING to inform you about situations that

could result in personal injury to yourself or other persons. Be
sure that all instrument operators read and understand the
precautions listed below. It is advisable to post a copy of the
WARNING precautions near or on the instrument shelf.
The following precautions must be observed when using the Series 200a:
• Do not use the detector in places where combustible gas or any source of fire or of
spark exists or might exist.
• Prior to connection, make sure that the voltage of the power socket into which the
detector power cable is plugged is the same as the power supply voltage indicated
on the detector.
• The type of the power socket into which the detector power cable is plugged should
be of a 3 prong type with a grounding terminal. Other types of power socket should
not be used.
• The accessory power cable should be used to connect the detector to the power
socket. Other cables should not be used.
• When using an organic solvent, wear eye protection. It is recommended that a sink
or the equipment for washing eyes be installed nearby, in case the organic solvent in
use comes into contact with the eye(s) or skin.
• When any abnormality, such as liquid leakage, is observed, turn off the power
immediately. Do not use the detector with the cover open; always unplug the
detector from the main power source before opening the cover.
• If the detector is used in a manner not specified by this users guide, the protection
provided by the detector may be impaired.
• When using a flammable organic solvent as a mobile phase, be sure to make the
necessary arrangements to prevent an accidental ignition (firing) by static electricity.
• If the fuse keeps blowing after being replaced, power off the detector immediately
and disconnect the power cord. Please consult your PerkinElmer Service
Representative.
We use the term CAUTION to inform you about situations that could
CAUTION
result in serious damage to the instrument or other equipment.
Details about these circumstances are in a box like this one. Be sure
that all instrument operators read and understand the
precautions listed below.
• External input/output are for contact closure. Never apply voltage to the External
input/output.
• As the detector is readily affected by the ambient temperature, use it in places where
there is little wind or change in the ambient temperature. Do not use the detector
near any source of vibration, electrical noise, or in places where corrosive gas and a
lot of dust exist.
• Do not connect any tube other than the provided “OUT tube” to the outlet tubing of
the detector. Put the exit end of the “OUT tube” in the waste liquid bottle and do not
apply back pressure.
• When connecting this detector to other detectors in series, put it at the end.
• If eluent freezes inside the detector, the flow line might break and damage the
detector. If there is any chance of eluent freezing during stoppage or storage of the
detector, withdraw the eluent completely from the flow line of the detector.
• When using any eluent containing a salt of high concentration, make sure that the
flow line is washed with water thoroughly after use. Failure to do so may result in
the plugging of the line causing the detector to cease operation.
• If the detector is not to be used for more than one week, then, prior to storage,
wash the flow line with pure water or acetone, and dry the line by allowing nitrogen
gas to flow through.
• Do not use any eluent which might corrode the material, such as stainless steel, that
it comes in contact with. Use of such eluent might cause a baseline drift and damage
the detector.
• Use a dry cloth to wipe the detector. Do not use water or alcohol. Use of such liquids
may damage the detector or erase characters or color on the panel.
NOTE: Do not connect any cord to the output signal terminal other than the attached signal cord.
NOTE: Use a thoroughly degassed eluent. It is recommended that a degassing device be used to
degas the eluent as it is easy to use and permits continuous degassing.
NOTE: If an eluent is replaced with another eluent in which it is insoluble, after having withdrawn
the old eluent from the line, wash the line with a solvent which is soluble in both eluents,
and then fill the line with the new eluent. For example, if water is replaced with chloroform,
wash the line with acetone before filling the line with chloroform.
NOTE: When replacing an eluent containing salt with an eluent containing organic solvent, wash the
line with pure water and then acetone before filling the line with the organic- solvent-
containing eluent. In contrast when replacing an eluent containing organic solvent with an
eluent containing salt , wash the line with acetone first and then pure water before filling the
line with salt-containing-eluent.
General Operating Conditions

The Series 200a has been designed and tested in accordance with PerkinElmer specifications
and in accordance with the safety requirements of the International Electrotechnical
Commission (IEC). The Series 200a conforms to EN/IEC 61010-1 (Safety requirements for
electrical equipment for measurement, control and laboratory use) as it applies to IEC Class 1
(earthed) appliances and therefore meets the requirements of EC Directive 73/23/EEC.
Only use the Series 200a indoors and under the following conditions:
Temperature 10 oC to 35 oC
Relative Humidity 20% to 80% relative humidity (non-condensing)
If possible, avoid any adjustment, maintenance and repair of the opened, operating
instrument. If any adjustment, maintenance and repair of the opened instrument are
necessary, this must be done by a skilled person who is aware of the hazard involved.
Whenever it is likely that the Series 200a is unsafe, make it inoperative. The Series 200a may
be unsafe if it:
• shows visible damage
• has been subjected to prolonged storage in unfavourable conditions
• has been subjected to severe transport stresses.

Environmental Conditions
Normal Operating Conditions:
• Indoors use
• The location must be free of smoke, dust, corrosive fumes, direct
sunlight and excessive vibration.
• Ambient temperature: +10°C to +35°C (+50°F to +95°F).
• Relative humidity: 20% to 80%, without condensation.
• Altitude: in the range 0 m to 2,000 m (sea level to 6,562 feet).
• Mains fluctuations not exceeding +/- 10% of the nominal voltage.
Safe Operating Conditions:

• Indoor use
• Ambient temperature: of +5oC to +40oC (+41°F to +104°F).
• Altitude: 0 m to 2000 m
Storage Conditions:
• Ambient temperature: –20°C to +60°C (–4°F to +140°F).

• Altitude: in the range 0 m to 12,000 m (sea level to 39,370 feet).
If the equipment is used in a manner not specified herein, the

protection provided by the equipment may be impaired.
WARNING
Electrical Safety
The instrument has been designed to protect the operator from potential electrical hazards.
This section describes some recommended electrical safety practices.
Lethal voltages are present at certain areas within the

instrument. Installation and internal maintenance of the
instrument should only be performed by a PerkinElmer Service
Representative or similarly authorized and trained person.
WARNING When the instrument is connected to line power, opening the instrument
covers is likely to expose live parts. Even when the power switch is off,
high voltages can still be present. Capacitors inside the instrument may
still be charged even if the instrument has been disconnected from all
voltage sources.
The instrument must be correctly connected to a suitable electrical supply. The supply must
have a correctly installed protective conductor (earth ground) and must be installed or
checked by a qualified electrician before connecting the instrument.
Any interruption of the protective conductor (earth ground) inside or

outside the instrument or disconnection of the protective conductor
terminal is likely to make the instrument dangerous. Intentional
interruption is prohibited.
WARNING
When working with the instrument:
• Connect the instrument to a correctly installed line power outlet that has a protective
conductor connection (earth ground).
• Do not operate the instrument with any covers or internal parts removed.
• Do not attempt to make internal adjustments or replacements except as directed in the

manuals.
• Disconnect the instrument from all voltage sources before opening it for any
adjustment, replacement, maintenance, or repair. If afterwards, the opened instrument
must be operated for further adjustment, maintenance, or repair, this must only be
done by a PerkinElmer Service Representative.
• Whenever it is possible that the instrument is no longer electrically safe for use, make
the instrument inoperative and secure it against any unauthorized or unintentional
operation. The electrical safety of the instrument is likely to be impaired if, for example,
the instrument shows visible damage; has been subjected to prolonged storage under
unfavorable conditions; or has been subjected to severe stress during transportation.
WEEE Instructions for PerkinElmer Products
or
A label with a crossed-out wheeled bin symbol and a rectangular bar indicates that the
product is covered by the Waste Electrical and Electronic Equipment (WEEE) Directive and is
not to be disposed of as unsorted municipal waste. Any products marked with this symbol
must be collected separately, according to the regulatory guidelines in your area.
The objectives of this program are to preserve, protect and improve the quality of the
environment, protect human health, and utilize natural resources prudently and rationally.
Specific treatment of WEEE is indispensable in order to avoid the dispersion of pollutants into
the recycled material or waste stream. Such treatment is the most effective means of
protecting the customer’s environment.
Requirements for waste collection reuse, recycling, and recovery programs vary by regulatory
authority at your location. Contact your local responsible body (e.g., your laboratory
manager) or authorized representative for information regarding applicable disposal
regulations. Contact PerkinElmer at the web site listed below for information specific to
PerkinElmer products.
Web address: http://las.perkinelmer.com/OneSource/Environmental-directives.htm
Customer Care: 1-800-762-4000 (inside the USA)

(+1) 203-925-4602 (outside the USA)
0800 40 858 (Brussels)
0800 90 66 42 (Monza)
Products from other manufacturers may also form a part of your PerkinElmer system. These
other producers are directly responsible for the collection and processing of their own waste
products under the terms of the WEEE Directive. Please contact these producers directly
before discarding any of their products.
Consult the PerkinElmer web site (above) for producer names and web addresses.
Features and Specifications
Features and Specifications 27
Features
The Series 200a is Deflection Type Refractive Index Detector with the following features.
Ease of use
• Full color liquid crystal display enables you to conveniently monitor the status of
Series 200a including the baseline.
• The built-in sequence makes your start up procedure simple. The detector now
automatically carries out those cumbersome and time-consuming processes such as
solvent exchange or baseline calculation.
Stability
The most sophisticated way of temperature control allows for a very fast start up time and a
fairly stable baseline performance is now available.
Safety
The solvent leak sensor generates an output signal that could trigger the pump flow to stop
in case of a solvent leak with the detector.
Integration
The well-organized Input/Output signal terminals and RS232C communication port provide
easy setup with your HPLC system.
Specifications
● Construction Deflection type
● Refractive Index Range 1.00 ～ 1.75
● Range 1/4 ～ 512 μRIU
● Linearity 600 μRIU
● Noise 2.5 nRIU ( Response : 1.5 seconds )
● Response Time 0.1 , 0.25 , 0.5 , 1.0 , 1.5 , 2 , 3 , 6 sec.
● Polarity Positive / Negative
● Auto Zero Optical & Electrical Auto-Zero
● Auto Zero Range All Refractive Index Range
● Auto Zero Resolution 1 ( @8mV /μRIU ) / 4 ( @2mV /μRIU ) nRIU
● Offset Range 0 ～ 500mV ( same with Integrator output sensitivity )
● Offset Resolution 10mV ( same with Integrator output sensitivity )
● Integrator Output 0 ～ 1V / FS ( Sensitivity : 2mV / μRIU , 8mV / μRIU )
● Recorder Output 0 ～ 10mV / FS
● Event Marker Marker out : 10 % of FS
● External Signal Input Auto Zero , Marker , Purge On / Off
( Contact Closure ) ( Contact Capacity : DC24V, 0.1A max.)
● External Signal Output (1) Ready
( Contact Closure ) (2) Solvent Leak
(3) Error ( One of following errors occurred )
Overheating
Null Glass Home Position
Optical Balance Error
Lost Parameters
Low Light Intensity
(Contact Capacity: DC24V, 0.1A max. )
● Temperature Control OFF, 30 ～ 50 °C ( 1 °C increments ); 77 °C Temp. Fuse
● Operator Support Automatic Start Up ( Start Up Sequence )
Span / Validation Guide
Real Time Baseline Monitor
● External Communication RS-232C
● Cell Volume 8 μl
● Maximum Flow Rate 10 ml/min ( mobile phase: pure water )
● Pressure Rating 50 kPa ( 0.5 kgf/cm2 )
● Internal Volume Inlet Port / Flow Cell: approx. 60μl
Flow Cell / Outlet Port: approx. 520μl
Total: approx. 580 μl
● Wetted Material SS316, Teflon, Quartz Glass
● Power Requirement AC 100 - 240 V ±10％, 50 / 60 Hz ±1%
● Power Consumption 150 VA (maximum)
● Dimensions 260 mm (W) x 200 mm (H) x 400 mm (D)
● Weight 13 kg (27 lbs)
● EMC Standards : EN 61326-1

● Safety Standards : EN 61010-1
Features and Specifications 29
Conditions to secure safety (EN 61010-1):

・Indoor use
・Altitude up to 2000m
・Temperature 5°C to 40°C
・Maximum relative humidity 80％ for temperatures up to 31 °C
decreasing linearly to 50％ relative humidity at 40 °C
・Transient overvoltages according to installation category II
・Pollution Degree 2 in accordance with IEC 60664
Detection Principle 31
Detection Principle
Optical System
In a refractive index detector of the deflection type, the light beam undergoes a deflection in
proportion to a difference in refractive index between one liquid and the other as it passes
through the two compartments of the flow cell, each being filled with a different liquid. The
deflection of the light beam is taken as a displacement on the light-receiving element and is
displayed as a difference of the refractive index.
Figure 1 Principle of detection of the deflection style
A variety of devices are provided in the optical system to enhance its operational stability and
make the detector more compact. The light beam from the light source passes through the
flow cell through the condenser lens, slit No. 1 collimater and slit No. 2. The light is reflected
by the mirror which exists just behind the flow cell and it forms and image of slit No. 1 onto
the photo sensor through the flow cell, slit No. 2 collimater lens and null glass. The photo
sensor has two light receivers placed side y side.
Figure 2 Optical System

When a difference develops in the refractive index between the sample and the reference in
the flow cell, the image on the photo sensor moves horizontally as illustrated in the following
figure (moves from (a) to (b)). The electric signals emitted respectively from the photo
sensor change in proportion to the extent of movement of the image. Thus, a signal
corresponding to the difference in refractive index can be obtained from a difference
between the two signal outputs.
Figure 3 Movement of the image on the photo sensor

Flowcell Connections
As shown in the following figure, the flow line is so designed as to enable the replacement of
a reference liquid by only pushing a button.
Figure 4 Flowcell Connections
When the PURGE button (solenoid valve) is in the ON position, NC is open and NO is closed,
thereby allowing an eluent to flow out of the sample cell to the waste liquid receptacle by
way of the reference cell.
When the button is in the OFF position, NC is closed and NO is open, thereby allowing the
eluent to flow from the sample cell directly to the waste liquid receptacle without going
through the reference cell.
Electronics Circuitry
An electrical circuit of Series 200a consists of a pre-amplification circuit, a signal processing
circuit (display and keyboard), a temperature control circuit and a light intensity control
circuit.
The temperature control circuit and light intensity control circuit are controlled by the signal
processing circuit.
Figure 5 Electrical System
Two photodiodes generate a photocurrent proportional to the amount of light. These

currents are, then, converted to the voltage signal at the pre-amplification circuit.
These voltage signals will, then, be sent to the signal processing circuit where their balance
will be precisely translated to the digital signal corresponding to the refractive index.
This digital signal will go through (1) Sensitivity calculation, (2) Response time calculation,
(3) Auto Zero / Off Set calculation, (4) Range attenuation for recorder output and (5) Event
Marker processing in their order and will finally be converted back to analog signal for
integrator output terminal and recorder output terminal.
Signal processing circuit

Signal processing circuit does also for the following jobs.
1. Parameter settings via Display or Keyboard (Recorder Range, Integrator Sensitivity,

Light Intensity Control, Temperature Control, Response Time, Polarity, Off Set).
2. Commands requested via Keyboard or external device (Purge On / Off, Event Marker
and Auto Zero: Optical Auto Zero is carried out by Optical Null Glass being driven
and fine adjustment is made by calculation).
3. Displaying data and status information such as Optical Balance, Refractive Indices,
Purge On /Off (status), Auto Zero (its duration), Temperature and Baseline.
4. Signal outputs (displaying various error messages such as Temperature, Light

Intensity, Auto Zero and contact closure signal for those output terminal).
5. Start-Up Sequence (automated the processes from the solvent exchange through
baseline equilibration).
6. Span adjustment and validation.
7. Data export and external parameter setting via RS232C port.
Light Intensity Control circuit controls a load current to the light source to keep Intensity
signal (sum of two I/V converters’ output voltage) constant.
This feature shall prevent the unexpected reduction in detector sensitivity due to such as
dirty flow cell, decreased lamp intensity.
Temperature Control circuit adjust an applied voltage for the heater to keep optical block
temperature constant based on the data form temperature sensor.
Instrument Functions and Touch Screens 39
Instrument Functions and

Touch Screens
Front Panel
5 6 7 8 9 10 11
12
4
13
14
3
Figure 6 Front panel of SERIES 200a
No Name of parts Function

1 Power Switch Press this key once to turn the unit on or off.
Leak LED
2 Illuminated when a solvent leak is detected.
[Leak]
Power LED
3 Illuminated when the power is on.
[Power]
4 LCD Display Liquid Crystal Display
Press the S.Seq key once to call Start-Up Sequence. If the S.Seq
key is pressed, Dialog box request to confirm whether you really
want to do the sequence. Press the Enter key to start the
sequence.
5 Start Up Sequence Key
[S.Seq] S.SEQ sign shall come up on the status bar. Press the Cancel key
if you don’t want the sequence. Pressing the S.Seq or the Cancel
keyduring the sequence shall suspend the sequence.
(See “Start-Up Sequence – Exit”)
No. Name of parts Function

Press the Purge key to turn the purge valve on or off to change
the flow path.
When the valve is on, the PURGE sign is highlighted by a yellow
Purge Key
6 background and the solvent flows through reference side of flow
[Purge]
cell instead of the sample side. Press the Purge to turn off the
valve. The Purge key isn't functional during the Start-Up
Sequence.
Auto Zero Key Press the Zero key to command "Auto-Zero". The Zero key
7
[Zero] doesn't function during the sequence.
Marker Key Press the Marker key to generate an event marker signal.
8
[Marker] (10% of Full Scale)
Arrow Keys Press the arrow keys to move cursor or to edit values.
9
Unless remarked specifically, the cursor shall scroll circularly.
Cancel Key Press the Cancel key to scrap the edited value
10
[Cancel] (cursor returns to the tab) or to cancel the command.
Enter Key Press the Enter key to save the edited value
11
[Enter] (cursor returns to the tab) or to confirm the command.
Outlet Port
12 The solvent passing through the flow path is expelled.
[OUT]
Inlet Port
13 The tubing from the separation column outlet is connected.
[IN]
14 Tube Holder Inlet Tube and Outlet Tube holders.
Back Panel
Figure 7 Back panel of SERIES 200a

Marker in terminals When these two terminals are short-circuited, the same actions
15
[MARKER IN] occurs as those when the marker button (8) is pressed.
Zero in terminals When these two terminals are short-circuited just as when the zero
16
[ZERO IN] button (7) is pressed, the automatic zeroing is actuated.
When these two terminals are short-circuited, the flow line of the
Purge terminals
17 eluent is changed just as if the purge button (6) is pressed,
[PURGE IN]
and both sample and reference cells are filled with the same eluent.
A contact pulse is sent out through these terminals at the same time
Error out terminals
18 as some error occurs. (Overheating/Null Glass Home Position/Optical
[ERROR OUT]
Balance Error/Lost Parameters/Low Light Intensity)
Leak out terminals A contact pulse is sent out through these terminals at the same
19
[LEAK OUT] time as a eluent leaks.
Ready out terminals A contact pulse is sent out through these terminals at the same
20
[READY OUT] time as the sequence is completed.

Signals to the data processing unit are sent out through
Integrator terminals these terminals. The sensitivity of the output signals is 2
21
[INTEG. OUT] mV/μRIU or 8 mV/μRIU (integrator range ; 500μRIU/V or
125μRIU/V)
Ground terminal
The shield of the provided signal cable should be connected
22 for cable shield
to this terminal.
[FG]
Recorder terminals Signals to the recorder are sent out through these
23
[REC.OUT] terminals. The sensitivity of the output signals is 10 mV/FS.
Communication port
24 The RS232C Communication port.
[COM]
Ground terminal These are the terminals to ground for the main body of the
25
detector.
The provided power cable should be plugged into this

26 Power connector
connector.
Side Panel
Figure 8 Side panel of SERIES 200a
In case of an internal solvent leak, the solvent will be

expelled from this port.
27 Drain Port Connect the Teflon tubing (6mm OD, 4mm ID).
Always have one end of the tubing go to a safe waste
disposal container.
Display
HOME Screen (Indicators and On-Screen Buttons)
This indicates the current state of optical axis balance by the

position of a blue colored box. Normally, the position of blue box
Optical
28 should be in the center. If it isn’t, press Zero key to do "Auto-
Balance
Zero". If it doesn't return to the center still, please contact your
PerkinElmer Service Representative.
This shows refractive indexes on a real time basis. When OVER

sign comes on, the refractive indexes are out of the following RI
range shown below. Press Zero Key to do "Auto-Zero". If OVER
Refractive
29 sign doesn't go off still, please contact your PerkinElmer Service
Index
Representative.
(Integrator range) -600～+600μRIU (512μRIU/V)
-150～+150μRIU (125μRIU/V)
Shows the general conditions of a Detector S.SEQ sign comes on

30 Status Bar when the sequence starts. The READY sign comes on when the
sequence is completed satisfactory.
31 Purge The PURGE sign will be highlighted when purge valve is on.
32 Zero The ZERO sign will be highlighted during Auto-Zero.

This shows the temperature reading in a real time basis.

33 Temperature When the difference becomes greater than + or – 1 degree,
the background color changes from green to yellow.
34 Baseline Shows the baseline in a real time basis.
Choose the “X axis” icon by or key.
Then, press or key to set the maximum span between
35 X-axis 4 and 60 minutes.
“X axis” data for the last 60 minutes from present is always stored
for your review.
Choose the “Y axis Origin” icon by or key.
Then, press or key to set the origin.
36 Y-axis
Minimum and maximum the value to be within the Refractive
Index range.
Choose the “Y axis” icon by or key.
37 Y-axis
Then, press or key to set the maximum span.
When the rudders and origin of a chart is changed, the default
setting for the chart is cancelled. Move the cursor to Reset by
38 Reset
or key and press the Enter key to resume the default
setting.
Move the cursor by or key and press the Enter key to

go to the Help screen.
39 Help Each screen has ？ button. Choose ？, and press the Enter key
to jump to the help topics corresponding to current screen.
Keep pressing or key to view other topics.
Shows the available menus to choose from.

The following list, below, is a list of tabs that you can choose
from.
Choose the desired tab by or key.
Then, press or key to go to that screen.
40 Tab
¾ HOME
¾ PARAMETERS (Parameter Setting)
● OPERATING : Operating Condition
● S. SEQUENCE : Start Up Sequence
¾ VALIDATION
To exit from the HOME screen, press the Cancel key to move cursor back to the tab.
PARAMETERS Screen (Operating Parameters)
1 TYPE “Analytical” Analytical type: Flow Rate: Do not Exceed 10ml/min！
Recorder range setting. Choose the field by or key and

2 Recorder Range
edit by or key.
Integrator range setting. Choose the field by or key and

3 Integrator Range
edit by or key.
Temperature setting. Choose the field by or key and

4 Temperature
edit by or key.
Response time setting. Choose the field by or key and
5 Response Time
edit by or key.
Polarity selection. Choose the field by or key and
6 Polarity
edit by or key.
Baseline shifting. Choose the field by or key and
7 Baseline Shift
edit by or key.
Language selection (English or Japanese）

8 Language
Choose the field by or key and edit by or key.
9 ？ Help
To exit from this screen, press the Enter Key to save those changes that you have made.
Press the Cancel Key to cancel those changes. In both cases, the cursor will move back to
the tab.
PARAMETERS Screen (Start Up Sequence)
Choose one of built-in sequences (Fine, Standard, Coarse) from

the menu or make your own (Custom).
1 Mode
Move cursor by or key to “Mode”.
Press or key to choose the mode.
This will repeat on/off of purge solenoid valve to remove air bubbles
trapped in the flow cell. The term of Purge Cycle is used for the
2 Purge Cycle length of one cycle. The default setting is 30 seconds. With this
setting, the valve will be on for 15 seconds and off for another 15
seconds. Choose preferred setting (Custom) if necessary.
3 Number of Cycle This will define the number of purge cycles.

At the end of purge cycles, Auto-Zero should be made.
This defines the length of the pausing period between the end of the
4 Time to Auto Zero
purge cycle and Auto-Zero. The default setting is 240 seconds.
Choose a preferred setting (Custom) when necessary.
This is a period in which the detector is equilibrated for the
measurement of drift and noise performance.
5 Equilibration Time
Choose one from the menu (80, 60 and 40 minutes) or enter
Custom value between 15 and 300 minutes.
This is a period in which the drift and noise performance is measured
after Equilibration.
6 Measuring Time
Choose one from the menu (80, 60 and 40 minutes) or enter
Custom value between 15 and 300 minutes.
7 Drift Set the target drift value here.
8 Noise Set the target noise value here.
9 ？ Help
To exit from this screen, press the Enter key to save those changes that you have made.
Press the Cancel key to cancel those changes. In both case, the cursor will move back to
the tab.
NOTE: The following table shows you the built-in sequences and the option range that you may
choose as your custom sequence.
Standard
1 Mode Fine Coarse Custom
(Default)
2 Purge Cycle 30 sec. 10～990 sec.
3 Number of Cycle 3 3～9
4 Time to Auto Zero 240 sec. 60～990 sec.
5 Equilibration Time 80 min. 60 min. 40 min. 12、15～300 min.
6 Measuring Time 80 min. 60 min. 40 min. 12、15～300 min.
7 Drift 100 nRIU/h 500 nRIU/h 2500 nRIU/h 50～9990 nRIU/h
8 Noise 50 nRIU 3～998 nRIU

Installation 51
Installation
Installation 53
Overview
This chapter describes the installation and connection procedures for the Series 200a.
This chapter covers the following installation and connection procedures:
• Power Connection and Grounding
• Tube Connections
• Operating Procedures
• Shipping List
• Spares Parts List

Power Connection and Grounding

1. Connect the detector to the power source according to the following procedure :
2. Confirm that the type of the power socket into which the detector power cable is
plugged is of a 3 prong type with a grounding terminal.
3. Confirm that the voltage of the power socket into which the detector power cable is
plugged is the same as the voltage indicated on the rear panel of the detector.
4. Turn off the power switch 1 of the detector.
5. Connect the detector to the power source using the accessory power cable.
Prior to connection, make sure that the voltage of the power socket into
which the detector power cable is plugged is the same as the power supply
voltage indicated on the detector.
WARNING
The type of the power socket into which the detector power cable is
plugged must be of a 3 prong type with a grounding terminal. Other
types of power socket should not be used.
WARNING
The accessory power cable must be used to connect the detector to the
power socket. Other cables should not be used.
WARNING
Do not use the detector in places where combustible gas or any

source of fire or of spark exists or might exist.
WARNING
CAUTION As the detector is readily affected by the ambient temperature, use it in

places where there is little wind or change in the ambient temperature .Do
not use the detector near any source of vibration, electrical noise or in
places where corrosive gas and a lot of dust exist.
Installation 55
Signal Cable Connections

Make correct connections using the provided signal cables in accordance with the figures
below.
1. When connecting to the integrator terminals:
2. When connecting to the recorder terminals:
3. When connecting to other terminals:
For the following cable connection, please use duplex lead wire.
External Signal Input Auto Zero, Marker, and Purge On/Off
Signal out Ready, Solvent Leak, and Error
To connect the wire
1. Scrape the sleeve off for about 7 to 8 mm.
2. Insert the end of the lead wire while pressing the button with a small flat screwdriver.
3. Then, remove the (-) driver to lock the wire.
4. Make sure the wire is firmly grabbed.
To release the wire
• Press the button with a small flat screwdriver to release the lock while pulling the
wire out.
CAUTION External input/output (Auto Zero-In, Marker-In, Purge-In, Ready-Out,

Leak-Out, and Error-Out) are for contact closure. Never apply voltage
to these connections.
Installation 57
Tube Connections
When connecting this detector to a high performance liquid chromatograph through the
tubes, wash the chromatograph and columns thoroughly with the eluent in use and make
sure that the eluent is clean before connecting the tubes.
Connect the tubes in accordance with the following procedures.
1. Connect the eluent inlet tubing 13 to the outlet of the column to be used through the
provided IN TUBE. When fastening the stainless steel set screw of the IN TUBE, grip the
eluent inlet tubing 13 with a wrench.
2. Connect the supplied OUT TUBE to the eluent outlet tubing 12.
CAUTION
Do not connect any tube other than the provided OUT TUBE to the outlet
tubing 12 of the detector. Put the exit end of the OUT TUBE in a waste
liquid receptacle and do not apply back pressure.
CAUTION
When connecting this detector to other detectors in series, connect this
detector at the end.
Operating Procedures
Use the detector in accordance with the following procedures.
[ Unattended Start Up ]
1. Set the parameter on operating parameter-setting screen.
2. Set the parameter on start-up sequence parameter-setting screen.
3. Start pumping mobile phase solvent at flow rate 1 ml/min.

If you can pump only at lower flow rate, you may set longer Purge Cycle.
4. Press S.Seq key once to call Start-Up Sequence. If S.Seq key is pressed, Dialog box
request to confirm whether you really want to do the sequence. Press Enter key to start
the sequence. S.SEQ sign shall come up on the status bar.
Elapsed time from the sequence start and total sequence time are displayed on the
screen.
5. Before measurement results become available, Equilibrate and Measuring is displayed

at Equilibration Time and at Measuring Time column respectively. As they become
available, drift and noise value will be displayed real time basis together with their target
values.
6. If drift or noise does not meet the target values within a defined period (Equilibration
Time), the detector will hold the valve at the on position.
7. the dialog box requests your command. Choose either the Repeat or the Ignore
button by or key. Confirm your preferred option by Enter. Choose Repeat
▲
to redo this equilibration normally. Otherwise, you may disregard results and let the
detector goes onto measuring by selecting the Ignore button.
8. If drift or noise does not meet the target value within the defined period (Measuring Time),
the dialog box will request your command. Choose either the Repeat or the Ignore
button by or key and confirm your preferred option by the Enter Key.
▲
9. Choose Repeat to redo the measuring normally. Otherwise, choose Ignore to abort
the sequence. Though the READY sign doesn’t come up, the detector is functional for
analysis.
10. As soon as drift and noise meet the target values during the measuring time, the
sequence is completed and the READY sign comes up on the status bar.
SERIES 200a is ready for the analysis.
NOTE: If you want to stop the sequence for whatever reasons, press the S.Seq key to suspend
the Start-Up sequence. The Dialog box will request to confirm if you really want to abort the
sequence.
Press Enter to abort the sequence. Press Cancel to resume the sequence.
Installation 59
[ Manual Start ]
1. Set the parameter on operating parameter-setting screen.
2. Start pumping mobile phase solvent at flow rate 1 ml/min to reference cell (Purge On).
3. Press the Purge key in every 10 seconds to on/off the purge valve for few minutes.
4. Keep pumping mobile phase solvent to reference cell for about 20 minutes from the
above step 2).
5. Press the Purge key to turn off the valve. Mobile phase solvent flows to sample cell.
6. Wait until the baseline is stabilized.
7. Press the Zero key to do an Auto Zero.
When using organic solvents, wear eye protection. It is recommended that

a sink or the equipment for washing eyes be installed nearby in case the
organic solvent in use comes into contact with the eye(s) or skin.
WARNING
When any abnormality, such as liquid leakage, is observed, turn

off the power immediately.
Unplug the detector from the main power source before opening
the cover.
WARNING
When using a flammable organic solvent as a mobile phase, be sure to make

the necessary arrangements to prevent an accidental ignition (firing) by
static electricity.
WARNING
If any eluent freezes inside the detector, the flow line might break which
CAUTION will damage the detector. If there is any chance of the eluent freezing
during stoppage or storage of the detector, withdraw the eluent completely
from the flow line of the detector.
When using any eluent containing a salt of high concentration, make sure that
CAUTION the flow line is washed with water thoroughly after use. Failure to do so may
result in the plugging of the line causing the detector to cease operation.
If the detector is not to be used for more than one week, then, prior to storage,
CAUTION wash the flow line with pure water and acetone, and dry the line by allowing nitrogen
gas to flow through.
Do not use any eluent, including hydrochloric acid, which might corrode the material,
CAUTION such as stainless steel, that it comes into contact with. Use of such eluent might cause
a base line drift and damage the detector.
NOTE: Use a thoroughly degassed eluent. It is recommended that a degassing service be used to
degas the eluent as it is easy to use and permits continuous degassing.
NOTE: If an eluent is replaced with another eluent in which it is insoluble, after having withdrawn
the old eluent from the line, wash the line with a solvent which is soluble in both eluents,
then fill the line with the new eluent. For example, if water is replaced with chloroform, wash
the line with acetone before filling the line with chloroform.
NOTE: When replacing an eluent containing salt with an eluent containing organic solvent, wash the
line with pure water and then acetone before filling the line with the organic-solvent-
containing eluent. In contrast, when replacing an eluent containing organic solvent with an
eluent containing salt, wash the line with acetone first and then pure water before filling the
line with the salt-containing-eluent.
Installation 61
《 Error Messages 》
To alert you about the situation that is hazardous or may cause a deterioration of your
analysis, SERIES 200a flashes the following five different error messages.
When any abnormality, such as liquid leakage, is observed, turn

off the power immediately.
WARNING
Please double-check if there is any solvent leak. In case you cannot fix problems, please
contact your PerkinElmer Service Representative.
Please contact your PerkinElmer Service Representative.

If you are commanding Auto-Zero via external control devise, refer to the procedure for
Optical Balance Error.
If you have this error when you turn on the Series 200a, please contact your PerkinElmer
Service Representative.
This indicates that Auto-Zero isn’t successful to get the optical zero point.
It may be caused by insufficient solvent exchange of reference flow path. Try purging
procedure to fill reference flow path with fresh solvent.
If you cannot still solve the problem, please contact your PerkinElmer Service representative.
This indicates that the parameters are lost.
Please contact your PerkinElmer Service Representative.
This indicates inadequate light intensity.
There are several possible causes for this error.
1. Different state of solvents between sample flow cell and reference flow cell. Purge the
reference side flow path with fresh solvent.
2. Air bubbles are in the flow cells. Repeat the purge on and off with flow, to remove the air
bubbles.
3. Optical axis is off from the center. Press Zero key to do an “Auto-Zero”.
Installation 63
Accessories
Items Shipped with the Instrument
When unpacking, please check if all the following accessories are contained:
No. Name Specification Quantity
Power cable Voltage is 100 or 120 V AC

1 1
Power supply cable set (125V) is shipped. For other voltages
the appropriate cable is also included.
2 Signal cable 2-core shield cable (approx. 2m) 1
Signal cable
3 RS-232 cable (approx. 2m) 1
control
Stainless steel tube of 1.6 mm outer diameter × 0.25 mm

Inner diameter × 500 mm length and 50 mm length of 1.6 x
4 IN tube set 1
1.0 Inner diameter (includes connection fittings)
Teflon tube of 2.5 mm outer diameter × 1.5 mm Inner

diameter × 1500 mm length
5 Outlet tube set 1
Time-lag type 3.15 A (T3.15A/250V), intended nominal

6 Fuse (0999-1629) 2
voltages (100-240 VAC)
1 2 3
4 5 6
Spares Parts Available
Part No. Description
0271-2273 Tungsten lamp

Maintenance 65
Maintenance
66
Maintenance 67
Overview
This chapter describes the routine maintenance procedures required to keep your instrument
in proper working condition and to ensure the highest possible level of performance.
You should perform only the maintenance procedures described in this chapter. If additional
maintenance is required, contact a PerkinElmer Service Representative.
This chapter covers the following maintenance procedures:
• Flow Cell Cleaning
• Replacing the Fuse
• Cleaning the Detector Exterior

Flowcell Cleaning
Depending on the solvents that you use, this cleaning procedure can vary. The following
procedure is a typical cleaning procedure.
1. Inject cleaning solution (acetone) by syringe from the inlet port (5 ml).
2. Inject deionized water by syringe from the inlet port (5 ml).
3. Inject nitric acid solution (15%) by syringe from the inlet port (5 ml).
4. Completely expel the nitric acid solution by flowing deionized water.
5. Exchange deionized water with the mobile phase solvent.
If buffers or solutions of high salt content have been in use, the cells may be contaminated
by precipitated salt. Large amounts of distilled, deionized water, such as 1 ml/min, for up to
several hours, is the simplest clean-up procedure.
Replacing the Fuse
Only use the same type and rating of fuse when replacing the
fuse 3.15A (T3.15AL/250V, Part Number 0999-1629).
WARNING
To remove fuse holder
1. Turn off the power of detector.
2. Remove the power cord. Push the lever located just above fuse holder cover.
Maintenance 69
3. Pull the holder out as you hear clicking sound.
To re-install fuse holder
1. Push the fuse holder in until the clicking sound is heard.
2. Make sure the holder is locked.
If the fuse is keeps blowing after being replaced, power off the detector
immediately and disconnect the power cord. Please call your
PerkinElmer Service Representative.
WARNING
Cleaning of the Detector Exterior

When the exterior such as top cover of front panel were stained or got wet for whatever
reasons, please keep the detector clean and dry by wiping off it by soft cloths or tissue
paper. If stains are hard to remove, try soft cloths absorbed a weak water solution of
kitchen detergent.
CAUTION
Use a dry cloth to wipe the detector. Do not use water or alcohol. Use of
such liquids may damage the detector or erase characters or color on the
panel.
Validation 71
Validation
Validation 73
From time to time, you are recommended to validate your HPLC system in
order to maintain the accuracy and credibility of your analysis. Series 200a
has a very unique and user-friendly feature for this cumbersome procedure.
200a
This indicates the difference between temperature setting and actual temperature
Temperature in Celsius. When the difference becomes greater than “+ or -” 1 degree
background color of this will be changed from green to yellow.
This indicates an applied voltage to the lamp. As it exceeds 4.5V, the background
color changes from green to yellow. Purge the reference side flow path with fresh
Lamp Voltage solvent at once. (Applied voltage to the lamp is automatically raised as light
intensity from the flow cell drops. In many cases the reduction of intensity is due
to dirt in the reference flow cell.)
This indicates accumulated service hours of lamp. When it exceeds 20,000 hours,
Lamp Hour the background color of this will be changed from green to yellow. Lamp
replacement is recommended.
Move cursor to Span by or key. Press the Enter key to go on “SPAN

VALIDATION” screen. (For the details of span validation, please refer span
Span
validation on the next page.) Press Cancel key to return to “VALIDATION”
screen.
Move cursor to Drift & Noise by or key. Push the Enter key to call Start-
Up Sequence to validate the detector according to the current parameters. The
Drift & Noise dialog box will request if you really want to do this. Press the Enter or the
Cancel key to confirm your preferred option. As the sequence starts, drift and
noise are measured and shown on “VALIDATION” screen.
？ Help
To exit the “VALIDATION” screen, press the Cancel key. The cursor will return to the tab.
Span Validation
To do Span Validation, follow this procedure.

1. Prepare the standard sucrose solution:
Weigh out 350 mg sucrose and transfer quantitatively to a volumetric flask.
Dissolve in the 100 ml deionized, filtered, degassed water and dilute to flask’s mark.
NOTE: Always use freshly made sucrose solution.
2. Equilibrate SERIES 200a by pumping deionized water through both the reference and
sample cells. Use the same deionized water as used to prepare the sucrose standard
solution. Start pumping at flow rate to 1 ml/min and do the Start-Up Sequence.
3. Make sure that the baseline is stabilized and the drift is equal to or less than 500 nRIU/h.
4. Press the Zero key to do Auto Zero.
5. Move cursor to the Baseline and press the Enter key to memorize the original (0μ RIU)
baseline. The cursor will move to the Span Line automatically after storing the original
baseline information.
6. Disconnect and remove the tubing from the inlet port of SERIES 200a.
7. Make sure that the purge valve is off.
8. Fill the syringe with the standard sucrose solution and gently inject the solution from the
inlet port.
9. As the baseline is stabilized, press the Enter key to measure.
10. The Validation screen will appear with the measured result.
11. The result should be within 487 ～ 537 (512μ RIU ±5%).
Troubleshooting 75
Troubleshooting
76
Troubleshooting 77
Overview
Use the following chart to troubleshoot any problems with the instrument. Should the
problem persist even after taking the corrective actions below, contact your PerkinElmer
Service Representative.
Troubleshooting Charts
Problem Possible Cause Solution
No power
(Lamps and displays 1. Power cable is not 1. Connect power cable to power socket.
do not go on when connected. 2. Replace fuse. (If new fuse blows soon after
the power button is 2. Fuse is blown. replacement, contact agent for repair.)
turned on.)
Baseline becomes Bubbles are present in

jagged. flow cell. Pass thoroughly degassed eluent through at flow
rate of about 1 ml/min. while pushing the PURGE
button repeatedly into ON and OFF positions for
sufficient period of time (until the baseline
stabilizes).
If an aqueous eluent is in use and the baseline
does not stabilize even after passing the degassed
eluent for more than one hour, it will be effective
to perform one passage of methanol.
1. Pulsation by pump is
Periodic noises are 1. Install device, such as damper and resistance
large.
generated. pipe to eliminate pulsation, close to delivery
2. Existence of bubbles
side of pump.
in the flow cell
2. Pass thoroughly degassed eluent through at
enlarges the pulsation
flow rate of about 1 ml/min. while pushing the
by pump.
PURGE button repeatedly into ON and OFF
positions for sufficient period of time (until the
baseline stabilizes). If an aqueous eluent is in
use and the baseline does not stabilize even
after passing the degassed eluent for more
than one hour, it will be effective to perform
one passage of methanol.
No periodic noises are 1. Pass thoroughly degassed eluent.

1. Insufficient degassing
generated. 2. Pass a 15% nitric acid solution. Be sure to
2. Flow cell is
wash with pure water after the use of nitric
contaminated.
acid.
3. Salts separated in the
3. Set PURGE button on and pump sufficient
tubing or flow cell.
amount of eluent that dissolves the salts and
4. Column is
wash with water.
contaminated.
4. Wash the column or use a new column.
Troubleshooting 79
Problem Possible Cause Solution

1. Replace the solvent thoroughly.
Large baseline drift (See the Maintenance chapter,
1. Insufficient solvent
Flow Cell Cleaning procedure.)
replacement.
2. Use detector in places where
2. Room temperature fluctuates
changes in room temperature
greatly.
are small.
3. Bubbles are present in flow cell.
3. Pass thoroughly degassed eluent
4. Flow line is contaminated.
through to purge bubbles.
4. Wash flow line thoroughly.
1. Push PURGE button into the
Low light intensity alarm 1. Insufficient replacement of the ON position and pump a
sufficient amount of eluent to fill
eluent in the reference cell.
the reference cell.
2. Bubbles are present in flow cell.
2. Pass thoroughly degassed eluent
through to purge bubbles.
Baseline drift occurs in Reference cell solvent has aged and Flush reference cell with mobile
few hours of start. deteriorated. phase.
Baseline will not zero Sample and reference cells do not Flush sample and reference cells
contain identical solutions. with mobile phase.
Flush sample and reference cells

Reference cell contains air bubbles.
with mobile phase.
Clean flow cell. See the Maintenance
Flow cell(s) dirty. chapter for the Flow Cell Cleaning
procedure.
Check for liquid in drain tube
Flow cell(s) damaged.
indicating a broken cell.
Deteriorating lamp or lamp out of

Check validation screen.
adjustment.
Appendix 81
Appendix
Appendix 83
Miscibility Chart of Solvents

UV Flash Boiling
Polarity Viscosity Refractive Fire Point Vapor
o
Cut-off Point Point
E2(Al2O3) (cP20 C) Index Density Gravity
(nm) (o C) (o C) Lower Upper (o C)
Fluoroalkanes -0.25- 1.250
n-Pentane 0.00 0.23 1.358 210 <-40 308.9 1.5 07.8 2.5 036.1
Hexane 0.00 1.375 210 -21.7 233.9 1.2 07.5 3.0 068.9
Isooctane 0.01 1.404 210
Petroleum ether 0.01 0.30 210
n-Decane 0.04 0.92 1.412 046.1 207.8 0.8 05.4 4.9 173.9
Cyclohexane 0.04 1.00 1.427 210 -20.0 260.0 1.3 08.0 2.9 081.7
Cyclopentane 0.05 0.47 1.406 210
Diisobutylene 0.06 1.411 210
i-Pentene 0.08 1.371 -17.8 272.8 1.5 08.7 2.4 030.0
Carbon disulfide 0.15 0.37 1.626 380 -30.0 100.0 1.3 44.0 2.6 046.1
Carbon tetrachloride 0.18 0.97 1.466 265
Amyl chloride 0.26 0.43 1.413 225 012.8 343.3 1.6 08.6 3.7 106.1
Butyl chloride 0.26 1.436 220 0-9.4 460.0 1.8 10.1 3.2
o-17.2 463.9 1.0 06.0 144.4
Xylene 0.26 0.62 － 0.81 ～ 1.50 290 m-25 527.8 1.1 07.0 3.7 138.9
p-25 528.9 1.1 07.0 138.3
i-Propyl ether 0.28 0.37 1.368 220 -27.8 443.3 1.4 21.0 3.5 068.9
i-Propyl chloride 0.29 0.33 1.378 225 -32.2 593.3 2.8 10.7 2.7 035.0
Toluene 0.29 0.59 1.496 285 004.4 536.1 1.4 06.7 3.1 110.6
n-Propyl chloride 0.30 0.35 1.389 225 <-17.8 2.6 11.1 2.7 046.1
Chlorobenzene 0.30 0.80 1.525 032.2 637.8 1.3 07.1 3.9 132.2
Benzene 0.32 0.65 1.501 280 -11.1 562.2 1.4 07.1 2.8 080.0
Ethyl bromide 0.37 1.424 511.1 6.7 11.3 3.8 037.8
Ethyl ether 0.38 0.23 1.353 220 -45.0 180.0 1.9 48.0 2.6 035.0
Ethyl sulfide 0.38 0.45 1.442 290
Chloroform 0.40 0.57 1.443 245
Methylene chloride 0.42 0.44 1.424 245 -50.0 518.9 3.8 15.4 2.2 038.5
Methyl i-butyl ketone 0.43 1.394 330
Tetrahydrofuran 0.45 1.408 220 -14.4 321.1 2.0 11.8 2.5 066.1
Ethylene dichloride 0.49 0.79 1.445 230 013.3 412.3 6.2 16 3.4 083.9
Methyl ethyl ketone 0.51 1.381 330 0-6.1 515.6 1.8 10 2.5 080.0
i-Nitropropane 0.53 1.400 380 048.9 420.6 2.6 3.1 131.1
Acetone 0.56 0.32 1.359 220 -17.8 537.8 2.6 12.8 2.0 056.7
Dioxane 0.56 1.54 1.422 260 012.2 180.0 2.0 22 3.0 101.1
Ethyl acetate 0.58 0.45 1.370 260 004.4 460.0 1.8 8 3.5 090.0
Methyl acetate 0.60 0.37 1.362 210 -10.0 501.7 3.1 16 2.6 060.0
Amyl alcohol 0.61 4.10 1.410 032.8 300.0 1.2 10.0 3.0 137.8
Dimethyl sulfoxide 0.62 2.24
Aniline 0.62 4.40 1.586 070.0 617.2 1.3 3.2 184.4
Dimethyl amine 0.63 0.38 1.387 275 <-17.8 312.2 1.8 10.1 2.5 056.7
Nitromethane 0.64 0.67 1.394 380 035.0 418.3 7.3 2.1 101.1
Acetonitrile 0.65 0.37 1.344 210 005.6 1.4 081.7
Pyridine 0.71 0.94 1.510 305 020.0 1.8 12.4 2.7 115. 0
Butyl cellosolve 0.74 220
i-Propanol n- 0.82 2.30 1.380 210 011.7 398.9 2.0 12 2.1 082.8
Propanol
Ethanol 0.88 1.20 1.361 210 012.8 422.8 4.3 19 1.6 078.3
Methanol 0.95 0.60 1.329 210 011.1 463.9 7.3 36 1.1 063.9
Ethylene glycol 1.11 19.900 1.427 210 111.1 412.8 3.2 197.2
Acetic acid Large 1.26 1.372
Water Large 1.333
* L. R. Snyder. Dekker. (Principles of Adsorption Chromatography)
Those in should not be employed

Appendix 85
Index
A I
About This Manual.................................................9 Installation
Accessories...........................................................63 accessories....................................................... 63
Appendix error messages................................................. 61
Miscibility Chart of Solvents ...........................83 grounding ........................................................ 54
manual start ..................................................... 59
operating procedures ....................................... 58
B power connection ............................................ 54
Back Panel............................................................43 signal cable connections.................................. 55
signal cable connections, wire connection....... 56
signal cable connections, wire release............. 56
C spares parts...................................................... 64
Circuitry................................................................36 tube connections.............................................. 57
Circuits unattended start up .......................................... 58
signal processing..............................................37 Instrument
Cleaning back panel ....................................................... 43
flowcell ............................................................68 front panel ....................................................... 41
Cleaning the Exterior............................................69 screen displays ................................................ 46
Conventions side panel ........................................................ 45
Notes, cautions and warnings ..........................10 touch screens ................................................... 46
text ...................................................................10
L
D Labels
Detector Exterior warning signs .................................................. 13
cleaning............................................................69 WEEE Instructions.......................................... 23
E M
Electrical Safety....................................................22 Maintenance
Electronics Circuitry.............................................36 cleaning the exterior ........................................ 69
signal processing circuit...................................37 flowcell cleaning ............................................. 68
Environmental Conditions ....................................21 fuse holder re-installation................................ 69
Error Messages .....................................................61 fuse holder removal......................................... 68
fuse replacement.............................................. 68
Manual Start......................................................... 59
F Miscibility Chart of Solvents ............................... 83
Features of the Series 200A..................................27
Flowcell Cleaning.................................................68 O
Flowcell Connections ...........................................35
Front Panel ...........................................................41 Operating Conditions ........................................... 20
Fuse Holder Operating Parameters........................................... 48
re-installation ...................................................69 Operating Procedures........................................... 58
removal ............................................................68 error messages................................................. 61
Fuse Replacement.................................................68 manual start ..................................................... 59
unattended start up .......................................... 58
Optical Features ................................................... 33
G
General Operating Conditions ..............................20 P
Grounding.............................................................54
Parameters screen
start up sequence ............................................. 49
H Power Connection ................................................ 54
Precautions.......................................................... 18
HPLC System
validation .........................................................73
R
Re-installing the Fuse Holder............................... 69
Removing the Fuse Holder................................... 68
Replacing the Fuse ............................................... 68
S T
Safety Information .............................................. 18 Touch Screens ...................................................... 46
electrical safety................................................ 22 operating parameters........................................ 48
environmental conditions ................................ 21 parameters........................................................ 49
Screen Displays.................................................... 46 start up sequence.............................................. 49
Screens Troubleshooting Charts ........................................ 78
operating parameters ....................................... 48 Tube connections.................................................. 57
parameters ....................................................... 49
Side Panel ............................................................ 45
Signal Cable Connections .................................... 55
U
wire connection ............................................... 56 Unattended Start Up ............................................. 58
wire release ..................................................... 56
Signal Processing Circuit ..................................... 37
Solvents V
Miscibility Chart ............................................. 83 Validation ............................................................. 73
Span Validation.................................................... 74 Validation Span .................................................... 74
Spares Parts.......................................................... 64
Specifications of the Series 200a ......................... 28
Start Up Sequence................................................ 49 W
Warranty................................................................. 8

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LIQUID CHROMATOGRAPHY

Part Number Release Publication Date

9936751 B Nov. 2005

Or emailed to: info@perkinelmer.com

Copyright © 2005 PerkinElmer, Inc.

Produced in the USA

PerkinElmer is a registered trademark of PerkinElmer, Inc.

The content of this User’s Guide is subject to change without notice.

About This Manual

Chapter 2 Safety Practices

Important safety information is provided in this chapter.

Chapter 3 Features and Specifications

This chapter contains features and specification of the Series 200a.

Chapter 4 Detection Principle

Chapter 5 Instrument Functions and Touch Screens

This chapter provides validation information.

The miscibility chart of solvents is in this appendix.

Conventions Used in this Manual

Bold text refers to text that is displayed on the screen.

Notes, cautions and warnings

Warning Signs on the Instrument

DANGEROUS VOLTAGES EXPOSED

FOR CONTINUED PROTECTION

We use the term WARNING to inform you about situations that

General Operating Conditions

Relative Humidity 20% to 80% relative humidity (non-condensing)

• shows visible damage

• has been subjected to prolonged storage in unfavourable conditions

• has been subjected to severe transport stresses.

Safe Operating Conditions:

• Ambient temperature: –20°C to +60°C (–4°F to +140°F).

If the equipment is used in a manner not specified herein, the

Lethal voltages are present at certain areas within the

Any interruption of the protective conductor (earth ground) inside or

When working with the instrument:

• Do not attempt to make internal adjustments or replacements except as directed in the

WEEE Instructions for PerkinElmer Products

Web address: http://las.perkinelmer.com/OneSource/Environmental-directives.htm

Customer Care: 1-800-762-4000 (inside the USA)

● EMC Standards : EN 61326-1

Conditions to secure safety (EN 61010-1):

Figure 1 Principle of detection of the deflection style

Figure 2 Optical System

Figure 3 Movement of the image on the photo sensor

Figure 4 Flowcell Connections

Figure 5 Electrical System

Two photodiodes generate a photocurrent proportional to the amount of light. These

Signal processing circuit

1. Parameter settings via Display or Keyboard (Recorder Range, Integrator Sensitivity,

4. Signal outputs (displaying various error messages such as Temperature, Light

6. Span adjustment and validation.

7. Data export and external parameter setting via RS232C port.

Instrument Functions and

Figure 6 Front panel of SERIES 200a

No Name of parts Function

4 LCD Display Liquid Crystal Display

No. Name of parts Function

Figure 7 Back panel of SERIES 200a

No. Name of parts Function

No. Name of parts Function

The provided power cable should be plugged into this

Figure 8 Side panel of SERIES 200a

No. Name of parts Function