MLT151 REVIEW Exam 1 CH 1-6

Clinical Laboratory Chemistry, 2nd Ed, Sunheimer ISBN 9780134413327

Be familiar with the following:

OSHA Automation
Chemical Hygiene Plan and labeling Throughput
MSDS Aliquots
Exposure Control Plan Clot detectors
BBP Carryover
PPE Open or closed reagent
Universal Precautions Incubation-heating
Testing Phases
MATH Preanalytical
Solutions % and M Analytical
Dilutions Postanalytical
Statistics-Mean, SD calculation Quality Assurance
Quality Control material
Clinical grade Water and reagents Westgard Rules
Labware types and uses Confidence Interval
Centrifuge types Accuracy
fixed angle Precision
swinging buckets Random and Systematic Errors
ultrafuge Analytical Range/Reportable Range
Cytofuge Reference Intervals/Expected Range
Heating devices Sensitivity
Thermometer types Specificity
Calibration
METHODOLOGY
Spectrophotometry and all aspects IMMUNOASSAY
Fluorescence, Luminescence Antigens and Antibodies
Chemiluminescence Haptens
Refractometry Competitive Immunoassay
Osmometry Sandwich Immunoassay
Electrochemistry-Potentiometry, ISE MEIA
Electrophoresis Chemiluminescence-luminol, dioxetene,
Chromatography acridinium
Flow cytometry EMIT-enzymes
Biosensors HOOK Effect

MOLECULAR
Basic concepts
Gel electrophoresis
DNA sequencing
Amplification