Results and discussion:

1. Phytochemical study by GC – MS:

The chemical composition of essential oil obtained from Lavandula multifida L. are listed
in Table 1. I this study, 62 compounds were identified and the major components were
Camphor (15.68%), 1,8- cinole (14.15%) and Alpha-Pinene.)%13.82( Previous studies on
Lavandula multifida chemicals cultivated in Tunisia such as Msaada et al, 2012 showed that
major components are linalool (50.05%), Camphene (10.06%), and study of chograni et al
showed that the dominant compounds in L. multifida essential oil growing wildly in Tunisia
were Carvacroal (31.815), B-bisabolene (14.89%) and acrylic acidester (11.43%). Whereas,
Saadi et al, 2016 showed that the major components of L. multifida essential oil growing in
Algeria was carvacrol (61.73%) in inflorescence and (50.92%) in leaves, flowed by linalool
(5.69%) in inflorescence and auethole (17.37%) in leaves. Another study done by Zinine et al
(2016) on L. multifida essential oils in Morocco identified the Carvacroal (65.6%) was the
major compound followed by Spathulenol (8.6%) and Para-Gymen-8-ol.
These differences in essential oils composition may probably due to environmental
conditions such as soil, light, temperature and humidity etc.

Table 1: Essential oil composition (% w/w) of Lavandula multifida.

%
No COMPOUNDS
(w/w)
1 Tricyclene 0.11
2 ALPHA-PINENE 13.82
3 Camphene 2.51
4 1,3,5-Cycloheptatriene, 3,7,7-trimethyl 0.13
5 beta-Pinene 0.99
6 beta-Myrcene 0.40
7 DELTA 3-Carene 5.43
8 alpha -Terpinene 0.12
9 Cymene 0.58
10 1,8-Cineole 14.15
11 Limonene 1.20
12 trans-beta-Ocimene 0.09
13 1,3,7-Octatriene, 3,7-dimethyl- 0.14
14 gamma-Terpinene 0.10
15 cis-Linaloloxide 0.11
16 ALPHA-TERPINOLENE 0.13
17 LINALOOL L 9.00
18 Camphor 15.68
19 BORNEOL L 6.04
20 Lavandulol 0.17
21 4-Terpineol 0.31
22 ALPHA TERPINEOL 2.40
23 Hexyl butanoate 0.12
24 Linalyl anthranilate 6.08
25 l-Bornyl acetate 1.84
26 LAVANDULYL ACETATE 0.80
27 Camphene 0.60
28 NERYL ACETATE 0.22
29 alpha-Copaene 0.50
30 alpha-Gurjunene 0.77
 31 trans-Caryophyllene 2.28
32 alpha-Humulene 1.71
33 trans-Caryophyllene 0.18
34 γ-Muurolene 0.11
35 Germacrene D 0.07
36 (+)-Epi-bicyclosesquiphellandrene 0.15
37 alpha-selinene 0.07
38 AROMADENDRENE VI 0.09
39 γ-Cadinene 1.77
40 1S,CIS-CALAMENENE 0.42
41 delta-Cadinene 1.11
42 ALPHA-CALACORENE 0.19
43 CYCLOHEXANE, CYCLOPROPYLIDEN- 0.21
44 Caryophyllene oxide 0.27
45 alpha-Caryophyllene 0.14
46 o-Menth-8-ene 0.33
Naphthalene, 1,2,3,4,6,8a-hexahydro-1-isopropyl-
47 0.74
4,7-dimethyl
48 Dehydroxy-isocalamendiol 0.30
49 Humulane-1,6-dien-3-ol 0.46
50 alpha-Cadinol 2.27
51 Ledol 0.30
52 1-Methylbicyclo[3.2.1]octane 0.12
53 Benzonaphthofuran 0.36
54 alpha-Bisabolol 0.64
55 DELTA.1,1'-Biindan 0.17
56 7-Methoxy-1,4,6-triazaphenothiazine 0.09
57 Isothujol 0.09
58 Tridecane 0.07
59 HAHNFETT 0.08
60 Tritetracontane 0.07
61 Octadecane 0.07
62 Hexatriacontane 0.09
99.54

2. Microbiological activity study: