VIRUS NOT ISOLATED - ANOTHER CLUE FROM IMMUNO-TEST DESIGN - Database Italia

07/07/2021 VIRUS NOT ISOLATED?
ANOTHER CLUE FROM IMMUNO-TEST DESIGN - Database Italia
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VIRUS NOT ISOLATED? ANOTHER CLUE FROM

IMMUNO-TEST DESIGN
By Matteo Martini
Last updated Oct 31, 2020 CORONAVIRUS HEALTH
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07/07/2021 VIRUS NOT ISOLATED? ANOTHER CLUE FROM IMMUNO-TEST DESIGN - Database Italia
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The task of the investigative journalist is to investigate and bring out truths that are not otherwise
obvious or wisely hidden. The task of the science journalist is to disseminate specialist topics to
the general public. An investigative science journalist should highlight and make known to the
public any scientific fraud that otherwise the "esotericism" of the so-called hard sciences would
not be understood or evident to the general public.
As we know there are now doubts about the isolation of the alleged new SARS-CoV-2 virus, doubts
first raised by the infectious disease specialist Fabio Franchi, by dr. Stefano Scoglio, also by the
biologist Pieter Borger 1 . An extensive discussion of the lack of evidence of isolation, admitted by
the researchers themselves (questioned in a stringent manner by two scientific journalists 2 ) and
by some official bodies (CDC, European Commission), as well as the numerous scientific
anomalies, are reported documented in our book Operation Corona: global coup 3. We therefore
limit ourselves here to observing a specific and more circumscribed data, relating to how the
immunological tests for Covid-19 were designed, highlighting how this can corroborate our
hypothesis.
An immunoassay involves the recognition of the antigen-antibody bond between a protein of the
pathogen (virus or bacterium) and an antibody of the host organism. Obviously the specificity of
this bond and the selectivity of the antibody for the investigated "species" is always relative, and
must be determined each time, in the validation process, in particular with respect to the
specificity parameter. This validation has never occurred in the case in question, neither for
molecular tests (RT-PCR) for SARS-CoV-2, nor for immunological tests, by the same admission of
official bodies. For example, the US FDA, specifically, behind the push of the "emergency" has
renounced any specification of sensitivity and specificity, and has simply introduced the very vague
concepts of "positive agreement" and "negative" 4 . The tests still in June 2020 had been adopted
with Emergency Use Authorization, therefore in the almost total absence of validation procedures,
and only on the basis of the data declared by the manufacturer 5 .
One of the main limitations, especially given the lack of isolation of the virus, and its failure to use
for the necessary standardization of tests (both molecular and immunological), lies in the fact that
at the moment the only test bench to validate immunological test systems it is the convalescent
serum, from which the immunoglobulins are taken and whose specificity should then be proved.
The first methodological limitation is precisely the fact that to date the same molecular RT-PCR
tests (the so-called "swabs") have not themselves been validated. The limits of the reliability of
molecular tests for Covid-19 were observed right away. A publication by Chinese researchers 6,
almost immediately withdrawn (and this does not surprise us very little), estimated a false positive
rate of up to 80%. Today we know that the average of asymptomatic patients is about 95% and this
may also be due to this. The rate of false positives, also based on the prevalence in Italy, is in fact
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about 95%, as estimated by the validation body FIND 7. On the basis of these numbers it is really
impossible to determine whether the "sick" or "convalescent", identified on the basis of these RT-
PCRs (after all, the clinic or even the X-ray is superimposed on other pre-existing diseases, for
example, respectively the flu or idiopathic interstitial pneumonias), whether or not they have had
contact with the presumed virus and whether it has a causal relationship with the clinical state.
The non-negligible problem of negative patients should also be remembered: already in China, as
elsewhere, patients considered covid- like but perfectly negative on tests have been observed 8(or
where you had to insist on repeating the test up to ten times to get a positive). Therefore we take
into account that on these fleeting bases the plasma to be used to provide and design the
immunoassay was identified.
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When the "novel coronavirus" mentioned in the vulgate told us by the media was discovered, much
was already known about bat zoonotic coronaviruses. Even too much. Bat coronaviruses have
been studied extensively since 2003 starting with SARS and have been the subject of research,
including gain of function research.which fueled the narrative of the pandemic itself having
provided the theoretical foundation (albeit "conspiracy") to explain the supposed greater
aggressiveness of a virus that usually in the human species is responsible for flu-like syndromes
(in the rare cases in which it is pathogenic ). In the background, in fact, the suspicion has always
remained that he had "escaped" from a "maximum security" laboratory like the one in Wuhan.
Studies of SARS, then MERS, and then all the possible SARS-like viruses that went hunting in the
biological reservoirs supposedly in the bats of the Yunnan caves, provided the narrative for the
current history of the pandemic. Despite the so-called "unpreparedness" for the pandemic,
however, it seems that we know almost everything that molecular biologists seem to need to know
to make the description of the virus as scientifically convincing as possible. It is that we already
know how the virus would enter the cells, through a spike protein of the envelope that binds, as in
all SARS coronaviruses-like the ACE2-receptor protein that allows entry into the cell. The natural
history of the "new" virus is therefore already known and was immediately so. In reality, the spike
protein was immediately well characterized both as proteomics and as genomics of the same 9 10 .
The mechanisms of action, however, were borrowed from those hypothesized for SARS. So it was
immediately clear that the main candidate in the role of antigen was the spike protein. Therefore,
our immunological tests have been designed with this target in reference.
Let's see how the molecular tests were designed and developed, obviously based mostly on ELISA
technology for the detection of IgA, IgG and IgM. An interesting article published in July 2020 in
'Nature' by Chee Wah Tane colleagues, A SARS-CoV-2 surrogate virus neutralization test based on
antibody-mediated blockage of ACE2 – spike protein – protein interaction , gives us an account of
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the process by which we arrived at the main immunological test of neutralizing antibodies against
the new coronavirus. We discover that the initial antibody test (to then develop a test with a
surrogate, i.e. only the antibody-binding protein) was performed not using the native SARS-CoV-2
virus as might be expected .but with a pseudovirus that simulates SARS-CoV-2, an artifact
prepared through an in vitro technique. It was not a coronavirus but a VSV (vesicular stomatitis
virus), which are sometimes used as vectors for attenuated vaccines. A VSV was made to infect
human-derived cells previously transfected with a plasmid bearing the gene sequence encoding
the viral spike protein of the new coronavirus, obtained from the famous sequencing published on
the GISAID database as early as mid-January 2020 11 . In this way a viral artifact was obtained,
from cell culture: VSV virus (not a corona), carrier of the spike protein. And it was this pseudovirus
that was used to fine-tune the binding to human antibodies. The explanation for this, officially, is
that this would have made the job easier, requiring level 2 rather than 3 (BSL-3) biosecurity
procedures in the laboratory. On the other hand, it is also true that this study takes place only at the
beginning, to study the possibility of antigen-antibody interaction, while the actual test, marketed
and carried out in hospital laboratories, provides only a fragment of the RBD (binding domain the
receptor) of the viral antigen, and is much faster and safer. The motivation for the greater ease of
work therefore seems a bit forced. It is unclear why researchers would not have used SARS-CoV-2
if it were actually available,
In reality, the use of a VSV-based pseudovirus is not brand new, and has already been done in 2017
for HIV 12 as well . This is not without relevance: in fact, as many know, there is a controversy, albeit
hidden from the general public, as to whether or not the HIV virus was really isolated (and not just
“sequenced”). This controversy pitted the late Nobel laureate Kary Mullis against the biologist
Peter Duesberg 13 14 . Certainly the use of pseudoviruses reinforces the suspicion of a physical
non-availability of the native virus, supposedly isolated.
Directions for pseudovirus preparation and validation for SARS-COV-2 are provided by J. Nie et al.
in a scientific article published as early as March 2020 15 . The pseudoviral artifact is also titled
and quantified: another extremely significant point, because the famous SARS-CoV-2, on whose
isolation we raised doubts, has never been titled or quantified (which in reality would be a
tautology: to isolate a virus really means establishing from what percentage onwards it can be said
to be "isolated" and therefore in fact being able to quantify it). This is confirmed by official
documents of the American CDC which wrote in July:
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" Since no quantified virus isolates of the 2019-nCoV are currently available" 16
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Therefore the virus, which would have been isolated from Zhu 17 and colleagues, has never been
titled. It is not clear why, in reality, the researchers in the "parent article" who tried to isolate it
(reporting electron micrographs that were not exactly satisfactory 18), did not quantify it or provide
operational indications to replicate the "isolation". Instead, we saw that all of this was done with
the packaged pseudovirus (which is not a coronavirus but a modified VSV virus) expressing only
the spike protein. Yet it would have been important to have the isolate of the real virus (titrated) in
order to validate the tests, both molecular and immunological. In fact, in the study by Chee Wah
Tan and colleagues only the spike protein was tested, assuming that it was the main target of
neutralizing antibodies, however testing the native virus would have reproduced more realistic
conditions, also allowing any interactions not foreseen by the initial hypotheses. .
On the other hand, the limits on the specificity of this type of test would remain, given that the
reported study compares the interaction with respect to SARS and MERS specific antibodies. It
would have been essential to know the cross-reactivity with the most common human
coronaviruses currently known (HCoV-229E, HCoV-NL63, HCoV-OC43 , HCoV-HKU1 ) responsible
for colds, which are the most frequent and widespread in the population, and which they are likely
to be responsible for many positive serological test cases. From the beginning, the problem was
known and an article in The Lancetreported that the non-negligible homology between the spike
proteins of the same family, in particular with the cold coronaviruses (homology that could reach
50-60%) would have allowed a significant cross-reactivity, which would have caused a high number
of positives , nullifying the biological significance of immunoassays 19 .
Returning to the focus of the article: we are faced with a further clue that so far there is no viral
isolate and everything that has been done so far in terms of experimentation has not involved
interaction with the native SARS-CoV-2 virus but only with artifacts, replicas, single cloned
proteins. For example, everything that happened in terms of investigation could have taken place
solely by having a sequence decided a priori, similar to that of other known coronaviruses, and by
producing the spike protein with recombinant techniques.
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Physical isolation (which inevitably involves purification and quantification to be defined as such)
is essential 1) to have the demonstration of the material existence of the virus (otherwise
identifiable only by the theoretical design of its genome) 2) to ensure that the sequenced genome
derives actually from the virus only and not a patchwork of heterologous material 3) to provide the
gold standard for the validation of subsequent tests.
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At the moment no scientific article has so far provided evidence of complete isolation or given
operational indications to replicate it. This is very serious when compared with the alarm and the
social consequences that certain policies deriving from the alleged emergency have brought
about.
1 ? https://www.researchgate.net/post/Has_SARS-
CoV2_been_isolated_purified_and_demonstrated_to_be_the_cause_of_COVID19
2 ? T orsten Engelbrecht and Konstantin Demeter
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antibodytests/
5 ? https://www.fda.gov/medical-devices/coronavirus-disease-2019-covid-19-emergency-use-
authorizations-medical-devices/vitro-diagnostics-euas
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For a more complete examination see also the excellent article by Franchi:
https://medium.com/who-cvd/pandemia-di-covid-19-igliamento-critica-dottor-fabio-franchi-
4846c4231e0c
6 ? https://pubmed.ncbi.nlm.nih.gov/32133832/
7 ? https://www.databaseitalia.it/i-tamponi-covid-19-producono-fino-al-95-di-falsi-positiva-
confermato-dallistituto-sup Superiore-di-sanita- Articolo-del-dott-stefano- rock/
8 ? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191555/
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10 ? https://www.microbiologyresearch.org/docserver/fulltext/jgv/64/12/JV0640122577.pdf?
expires=1603828925&id=id&accname=guest&checksum=D96D4A476D23FE14ECFACE7AB5B41652
11 ? https://www.ncbi.nlm.nih.gov/nuccore/MN908947
12 ? https://pubmed.ncbi.nlm.nih.gov/28933644/
13 ? https://it.wikipedia.org/wiki/Peter_Duesberg
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14 ? See: P. Duesbeg, Aids il Virus Inventato, a popular text in which, in addition to the question of
non-isolation, the viral hypothesis of AIDS is contested. https://www.macrolibrarsi.it/libri/__aids-il-
virus-inventato-libro.php
15 ? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144318/?
fbclid=IwAR3U98pOv0rZwOJNtf_sQ53ZwdBOf7qo32Q5qdp00eBSED7DtoqBj1_pzak
16 ? https://www.fda.gov/media/134922/download
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18 ? See . Franchi, op.cit .
19 ? https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(20)30788-1/fulltext
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 immuno test immunological test Virus isolated
Matteo Martini
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Matteo Martini, is a doctor in Pharmaceutical Chemistry and Technology, and studied History of
Science and Philosophy of Science at the La Sapienza University of Rome. He deals with
scientific dissemination, counter-information and natural medicine. He was professor of
Functional Medicine and Orthomolecular Nutrition at the Giordano Bruno Popular University in
Rome. He also collaborated with the international magazine Nexus.
Telegram channel: https://t.me/ilVeritiero
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VIRUS NOT ISOLATED? ANOTHER CLUE FROM

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2 ? T orsten Engelbrecht and Konstantin Demeter

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