Activity #1

THE MICROSCOPE
Merielle Sanja D. Ang
Student’s Name _________________________ March 30, 2022
Date Performed _____________
BS PSYC 1st Year A70C
Course/Year/Section _____________________ March 30, 2022
Date Submitted _____________
Instructor’s Name ________________________
LIMBAUAN, JARYNA OBUYES

I. A. Label the parts of the microscope

Eyepiece

Revolving Eyepiece

Objective Lenses

Stage Clip

Stage

Mirror

MIrror Rack

Coarse Adjustment Knob

Fine Adjustment Knob

Compound Light Microscope

B. Magnification

Eyepiece X Objective = Total Magnification

Scanner 10x
_______ x 4x
________ = 40x
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LPO 10x
_______ x 10x
________ = 100x
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HPO 10x
_______ x 40x
________ = 400x
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Oil immersion 10x
_______ x 100x
________ = 1000x
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C. Draw letter "e"

1. Normal position right side- up

2. Under low-power objective upside-down & backwards,

covering a wider field.

3. Under high-power objective

increase in mag. of lens power means filed
of view gets smaller with the image getting larger.

D. The Ocular Micrometer

1. 1. Compute for the calibration constant of your ocular micrometer. Show

your solution.
100 stage div. x 10 mm = 1000 mm
Under LPO: 1 ocular div. x1000mm = 100 ocular div. x (?) mm
1000 mm/100x = 10 mm

10 stage div. x 10mm = 100 mm

Under HPO: 1 ocular div. x1000 mm = 100 ocular div. x (?) mm
1000 mm/400x = 2.5 mm

1.2. How do these compare with each other?

The calibration of HPO is higher than that of the LPO

2.1. Determine the width (in μm) of the Paramecium (same area). Show your
solution.

Using LPO: 1 div. = 10 mm

19 mm x 10 mm = 190 mm

Using HPO: 1 div. = 2.5 mm

76mm x 2.5 mm = 190 mm
IF. 77 mm x 2.5 mm = 192.5 mm

2. 2. How do these compare with each other?

Even in different magnification of the Paramercium, its width will be approximately be the same.
II. Guide Questions:

1. What is the correct procedure for carrying the microscope?

In order to avoid any mishaps or accidents, it is important to remember to hold the miscroscope with one hand around
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the arm or its handle while the other hand is under the base for support. It is the most correct and secure way to hold
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walking with said microscope. It is also important to avoid touching the lenses when doing so.
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2. Compare the image seen in the compound microscope and dissecting

microscope in terms of magnification, dimensions, inversion of image
and direction of movement. Tabulate your answers.

compund 40x, 60x, 400x 2. dimensional inverted lens could be rotated

microscope 1000x view

Dissecting between 5x & 50x 3. dimensional one lens is unmovable

Microscope not inverted
view and could be zoomed

3. How does one improve contrast when using a dissecting microscope?

One could adjust the field iris diaphragm to improve the contrast and even the resolution of a specimen. Placing suitable
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filters or even aperture within the optical path in the illuminating system may also improve its contrast (Sanderson, 2000)
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