PROCEDURE
1. Place the paper lining clean side out
on a table or any clean, flat surface
away from the client.
2. Spread the plastic lining over the
paper lining, clean side in.
3. Place the bag in the area away from
the client, tucking handles beneath
the bag.
4. Open the bag; take out soap in dish,
towel and apron.
5. Do thorough hand washing. Dry
hands, place soap/dish and towel
near the bag.
6. Put on apron, right side out.
7. Take out the kidney basin and place
3 Cotton balls with alcohol.
8. Take out 2 droppers, a test tube and
holder, and place these in the
kidney basin. Take out the alcohol
lamp, match .Benedict’s solution
and place them on the work area.
9. Place the waste receptacle at one
corner of the work area.
10. Light the lamp.
11. Place 6-8 drops of urine in the test
tube.
12. Add 3-5 ml. Of Benedict’s solution to
the urine
 Used droppers should be placed
near the waste receptacle.
13. Pass the test tube with mixture over
the flame, mouth of the TT should
be away from you and the client.
RATIONALE
 Paper lining reduce the risk of
splitting edges.
 To protect the materials from
contamination.
 To promote aseptic technique.
 To make a non-contaminated work
field or area.
 To prevent the risk of cross-
contamination.
 Tucking handles beneath the bag, so
as not to be a hindrance when
things are taken from the bag.
 To prepare for handwashing.
 To promote aseptic technique and
prevent the spread of
microorganism.
 To protect the nurses’ uniform.
Keeping the crease creates aesthetic
appearance.
 To make them readily accessible
 To save time and effort
 To make them readily accessible
 To save time and effort
 To promote proper waste disposal 
 To prepare for the heating in
Benedict’s test.
 To obtain accurate result
 To obtain accurate result
 Test tube must be pointed away
since heating liquid may shot out of
the test tube.
14. Remove from flame and stand for
one minute. Put off flame.
15. Interpret:
Clean blue to marked cloudy green
———– negative
Bluish green———————— +
Yellowish green———————++
Yellow——————————— +++
Orange——————————— ++++
16. Wash the TT, TT holder and
droppers with soap and water.
Drain, place them in the work area.
17. Disinfect the kidney basin, TT, TT
holder and droppers with 3 pieces
of CR with alcohol, Discard 2CB.
Place the articles inside the kidney
basin(Save the last CB with alcohol)
18. With one CB with alcohol, disinfect
the lamp and the plastic lining.
19. Discard the waste receptacle.
20. Do thorough hand washing. Dry
Hands with the towel.
21. Return all articles in their proper
places in the bag.
22. Remove apron away from the body,
folding contaminated part in. Place
over the flaps of the bag.
23. Fold the plastic lining contaminated
part in. Place over the flaps of the
bag.
24. Hold the bag with one hand, and
with the other hand fold the paper
lining , contaminated part in. Place
over the plastic lining.
25. Close the bag.
 To prevent burn injuries
 To prevent the spread of
microorganisms and promote
cleanliness.
 Prepares equipment for future use
 Reduces microorganism transfer
among co-workers;
 Ensures asepsis
 Proper disposal of used materials
reduces the risk for infection
transmission and contamination of
other items.
 Hand washing prevents the spread
of microorganism.
 Moisture in hands may cause
contamination of microorganisms.
 Prepares equipment for future use
 To prevent contamination and
infections.
 To ensure that the contaminated
part is contained.
 Ensure asepsis
 To protect the bag from getting
contaminated.
 To secure the materials inside the
bag.