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ABSTRACT
3
Corresponding author. FAX: +61 8 9266 2958; EMAIL: v.jayasena@curtin.edu.au
PRACTICAL APPLICATIONS
INTRODUCTION
Sample Collection
Crimson Seedless table grapes (Vitis Vinifera L.) were collected from two
commercial table grape growers in Gingin West (31.19 S, 115.58 E) and
Henley Brook (31.80 S, 116.00 E) in Western Australia. Twenty kilogram
samples of grapes, consisting of a range of clones, were obtained at 1-week
intervals from February 10 to March 10, 2005 to get berries with a wide range
of acid and sugar contents. The samples were packed in 10-kg cartons with a
polythene liner. A UVASYS sulfur dioxide generator pad (Grapetek Pty Ltd.,
Epping, South Africa) was placed on top of the grapes in cartons to reduce
spoilage. Cartons were placed in a cold storage operating at 2C until the
sampling was complete.
Density Separation
At the end of sampling period, all of the samples were taken out of the
cold storage and placed at room temperature (25C) overnight. The samples
were pooled, and the bunches were trimmed into single berries with a short
length of pedicel still attached. Uniform berries were systematically floated in
a series of sugar solutions at 25C to sort them into groups that were one °Brix
apart. Solutions used included 21°, 20°, 19°, 18°, 17°, 16° and 15°Brix. Berries
were poured into the highest °Brix sugar solution, and the berries that floated
were removed and poured into the next solution, which was one °Brix lower in
concentration. The procedure was repeated for the remaining solutions.
Berries that sank to the bottom of each solution were removed and washed to
remove any remaining sugar. The washed berries were dried using paper
towels. A minimum of 120 berries was collected from each oBrix level.
CONSUMER ACCEPTABILITY OF TABLE GRAPES 739
Acidity
Titratable acid content was determined by using the method described by
Iland et al. (1993). A 10 mL sample of juice was titrated with 0.1 n sodium
hydroxide to an end point of pH 8.2 using TPS digital pH meter (TPS Pty. Ltd.,
Springwood, Qld., Australia). The titrated volume of 0.1 n NaOH was
recorded, and the acidity was calculated as tartaric acid content (g/100 mL of
juice) by using the following formula:
°Brix/acid Ratio
The °Brix to acid ratio for each sample was calculated by dividing the
°Brix value by % acidity.
Sensory Evaluation
The sensory panel consisted of 63 participants representing general grape
consumers. The tasting sessions were carried out in a lighting-control room
with specially designed lightning to mask the skin color of samples. The
sensory booths were individually separated from one another to prevent com-
munication between panelists. Panelists were asked to indicate their prefer-
ence on a 9-point Hedonic scale with degree of liking: 1 = dislike extremely,
2 = dislike very much, 3 = dislike moderately, 4 = dislike slightly, 5 = neither
like nor dislike, 6 = like slightly, 7 = like moderately, 8 = like very much and
9 = like extremely. In each session, five different samples were given to rate
the sweetness, sourness, crispness, flavor and overall acceptability of samples.
The percentage of consumer acceptance was calculated using the following
equation:
Statistical Analysis
Data were analyzed for analysis of variance using SPSS 13.0 (SPSS Inc.,
Chicago, IL), and means were compared by using least significant difference
test at 5% level. The correlations among instrumental measurements and
sensory parameters were studied by means of Pearson’s correlation matrix.
9
8 A
Degree of liking (1-9)
7
6 a a
5 b b
c
4
3
2
1
0
100
Consumer acceptance (% )
80
B
60
40
20
0
16.0-1 7.0 17.1-18 .0 18.1-19 .0 19.1-20 .0 20.1-21. 0
°Brix range
9
8 A
Degree of liking (1-9)
7
6
a
b b
5 c d e
4 ff
3
2
1
0
100
Consumer acceptance (%)
80
B
60
40
20
0
0 .5 0- 0 .5 6- 0 .6 1- 0 .6 6- 0 .7 1- 0 .7 6- >0.80
0 .5 5 0 .6 0 0 .6 5 0 .7 0 0 .7 5 0 .8 0
% Acidity range
acidity of the berries ranged from 0.51 to 0.88%. Consumers degree of liking
(based on “overall acceptability” scores) varied from “dislike slightly” for
berries with acidity >0.80% through “like slightly” for berries within the
acidity range from 0.61–0.80% to “like moderately” for berries within the
acidity range of 0.50–0.60% (Fig. 3a). Consumer acceptance of Crimson
Seedless table grapes increased from 33 to 90%, with the decrease in acidity
from 0.80 to 0.50% (Fig. 3b). There was a significant (P ⱕ 0.05) increase in
the degree of liking of berries with a decrease of 0.05% in acidity (Fig. 3a).
The acidity demonstrated a negative correlation with sensory attributes. It
demonstrated correlation coefficients of determination (r2) of 0.77, 0.77, 0.55
and 0.74 with sweetness, sourness, crispness and flavor attributes, respectively
(Table 1). The overall acceptability of Crimson Seedless table grapes exhibited
a negative correlation (r2 = 0.79) with berry acidity (Fig. 4), which is quite
higher than that of °Brix value (r2 = 0.58). Acidity also demonstrated better
TABLE 1.
MATRIX OF CORRELATION COEFFICIENTS OF DETERMINATION (R2) FOR SENSORY ATTRIBUTES AND INSTRUMENTAL
MEASUREMENTS OF CRIMSON SEEDLESS TABLE GRAPES
Acidity °Brix °Brix/acid ratio Sweetness Sourness Crispness Flavor Overall acceptability
9
8 A
Degree of liking (1-9) 7
6
a
b
5 c
4
d
e
3
2
1
0
100
Consumer acceptance (%)
B
80
60
40
20
0
<20 20.1-25.0 25.1-30.0 30.1 -35.0 3 5.1-40.0
°Brix/Acidity range
4
14 16 18 20 22
°Brix
°Brix/acid Ratio
Consumers degree of liking (based on “overall acceptability” scores)
varied from “dislike slightly” for grapes having °Brix/acid ratio of <20 through
“like slightly” for grapes within the °Brix/acid ratio range of 20.1–30.0 to “like
moderately” for grapes within the °Brix/acid ratio range of 30.1–40.0
(Fig. 5a). Consumer acceptance of Crimson Seedless table grapes increased
from 33 to 87% with the increase in °Brix/acid ratio from 20 to 40 (Fig. 5b).
There was a significant (P ⱕ 0.05) difference in the degree of liking of grapes
with a change of 5 in °Brix/acid ratio (Fig. 5a).
The sensory ratings showed that the °Brix/acid ratio is a better predictor
of sensory attributes as compared with °Brix or acidity alone. It demonstrated
high correlation coefficients of determination (r2) of 0.85, 0.83 and 0.81 for
sweetness, sourness and flavour attributes, respectively (Table 1). The overall
acceptability of Crimson Seedless table grapes can be well predicted using
°Brix/acid ratio, as it demonstrated the highest level of correlation of r2 = 0.85
(Fig. 6) compared with °Brix (r2 = 0.58) or acidity (r2 = 0.79) alone. The
overall acceptability (value ranges from 1–9 according to a 9-point Hedonic
scale) of Crimson Seedless table grapes can be predicted using the following
equation:
8
y = -6.68x + 10.53
Overall acceptability (1-9)
2
7 r = 0.79
4
0.4 0.6 0.8 1.0
Acidity (%)
8
y = 0.11x + 3.01
Overall acceptability (1-9)
2
7 r = 0.85
4
15 20 25 30 35 40
°Brix/acid ratio
correlation between sugar/acid ratio and flavor preferences, and suggested that
sugar/acid ratio should be used as basis for determining fruit ripeness. Simi-
larly, studies by Crisosto and Crisosto (2002) demonstrated that consumer
acceptance of “Redglobe” table grapes is more sensitive to °Brix/acid ratio
than °Brix alone. Compared with °Brix alone, °Brix/acid ratio also demon-
strates higher degree of association with sensory attributes of some other
CONSUMER ACCEPTABILITY OF TABLE GRAPES 747
fruits, such as apples (Harker et al. 2002a) and appears to be very useful as a
grapefruit maturity indicator (Fellers 1991). Consumer acceptance (%) and
degree of liking in peach and nectarine cultivars increased with the increase in
soluble solid concentration (°Brix), but it also depended on their acid contents
(Crisosto and Crisosto 2005).
In contrast to our findings, some of the researchers have shown that
°Brix/acid ratio is a poor predictor for sensory quality of grapes. Combrink
et al. (1974) found the sugar acid ratio to be less reliable than °Brix alone for
South African condition and recommended the combination of °Brix readings
and grower taste to determine the grapes’ quality. Somers (1975) stated that
°Brix, or acidity, or °Brix/acid ratios were inadequate for determining harvest
time of red wine grapes under Australian condition. °Brix/acid ratio was
proved to be ineffective in determining sensory quality in low-acid grape
varieties (Guelfat-Reich and Safran 1971). Ginsburg et al. (1973) argued that
in using the °Brix/acid ratio method, the maturity of grapes is expressed as a
ratio and therefore does not guarantee any standard. They quote the following
example to illustrate their point: grapes with an acid content of 1% and a
soluble solid contents (°Brix) of 18% will have a ratio of 18:1, while grapes
with a soluble solids contents of 9% and an acid content of 0.5% will also have
a ratio of 18:1. Organoleptically, these samples will differ widely, although the
ratios are identical. Although we agree with their argument, it does not change
our conclusion, since this method is only applicable for a given range of
soluble solids concentration and acidity and for the given cultivar of grapes.
Similarly, Crisosto and Crisosto (2002) found a high correlation between
consumer acceptance and sugar : acid ratio in the case of Redglobe table
grapes, but within a certain range of acidity or sugar contents.
BrimA is a scale that has recently been proposed as a more sensitive
predictor of consumer acceptability in different fruits (Jordan et al. 2001). It is
calculated using the formula: BrimA = °Brix - k ¥ total acid, where k is a
constant and its value reflects the tongue’s higher sensitivity to acid than to
sugar. The value of k may vary between fruit species/cultivars due to different
mixes of acids and sugars, but usually ranges from 2 to 10. The k value for
citrus and grapes was found to be around 5 (Jordan et al. 2001). In the present
study, BrimA did not improve the prediction of sensory attributes over that
obtained by °Brix/acid ratio. During determination of the value of k using our
data set, we found that correlation with overall acceptability improved pro-
gressively as k increased from 2 to 10, but it could not achieve the r2 value
obtained by °Brix/acid ratio. With k = 2, r2 value was 0.58, and it increased to
0.78 when k = 10, and this was far below the r2 value of 0.85 obtained using
°Brix/acid ratio (Fig. 6). BrimA has also not improved the prediction of
sensory attributes of apples over those obtained using °Brix/acid (Harker et al.
2002b). Hence °Brix/acid ratio method is useful for determining the best time
748 V. JAYASENA and I. CAMERON
to harvest once the grapes have developed a berry color typical for the variety
and have a soluble solids concentration of >16°Brix. Coombe et al. (1980)
advocates that the sugar/acid index is robust because it relates to natural
phenomenon, has a logical connection with quality and operates wherever the
fruit is grown.
CONCLUSIONS
°Brix and titratable acidity are simple objective tests and can be exploited
for determining quality of Crimson Seedless table grapes. °Brix or titratable
acidity alone or even BrimA are relatively poor indicators of consumer accept-
ability. However, the °Brix/acid ratio is highly correlated with consumer
acceptability and can be used as an efficient instrumental method to determine
consumer acceptability of Crimson Seedless table grapes. This will enable the
Crimson Seedless table grape growers to decide when to begin the harvesting
of the fruit, by choosing a representative sample and determining the °Brix/
acid ratio. The best time to harvest Crimson Seedless table grape will be when
most of the berries attain °Brix/acid ratio of 35–40.
ACKNOWLEDGMENTS
This projected was funded by the Australian Table Grape Association and
Horticulture Australia Limited. The study was a joint project between the
Department of Agriculture and Food Western Australia and Curtin University
of Technology.
REFERENCES
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KOSTO, I. 2002. Factors affecting taste scores of early season seedless
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WINKLER, A.J. 1958. The relation of leaf area and climate to vine perfor-
mance and grape quality. Am. J. Enol. Vitic. 9(3), 10–23.