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PLANT

BIOTECH
NOLOGY
AZIZ
PURWANTORO
HERO

Plant
Breeding

Zero
Application of Biotechnology on Plant Breeding

• Limitation on conventional breeding


Background • Advance on Genetics

• Plant Tissue culture


Basic Tools • DNA’s knowledge

• Genetic transformation
Methods • Genome Editing
Conventional Modern
Aspects
Breeding Breeding
Source of gene : Narrow Broad

Time consume : Long Short

Labors cost : High Low

Methods : Simple Complicated

Precision : Bias Unbias

Reliability : Unfixed Fixed


PLANT TISSUE CULTURE
Scientific • Cell Teory
reason • “Totipotency”

• Mass
Purposes Propagation

• Sterile
Requirements • Handling with
care
Eksplan Medium PGR Purposes

• Leaf • MS • Auksin • Organogenesis


• Ovarium • NP • Sitokinin • Embryogenetis
• Anther • Gamborg • GA3 • Kalus

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