PAMANTASAN NG LUNGSOD NG MAYNILA

(University of the City of Manila)

COURSE SYLLABUS

A. University Vision and Mission

B.1. University Vision
Guided by the values of academic excellence, integrity, and social responsibility, PLM endeavors to be one of the leading
universities in the ASEAN.

B.2. University Mission

The PLM Board of Regents, Management, faculty, and staff are committed:
• To be recognized by Philippines and ASEAN academic accrediting agencies as a premier university for its quality
education, research and extension services.
• To ensure that PLM maintains a higher than national average performance on all professional licensure
examinations taken by its graduates.
• To continue to provide the students with an education that will give them a competitive advantage from
employment opportunities.

B. College of Science Vision and Mission

B.1. College Vision
To make PLM a premier university, a Center of Excellence and frontier of Knowledge in science through rigorous academic
training in teaching, research, and overall scholarship that remains relevant to the demand of the 21st century. By
responding to the challenges, needs, and on-going progress of the Filipino people, neighboring countries in Asia and the
world at large. PLM will proudly display its innovative brand of leadership both globally and technologically.

B.2. College Mission

As a Center of Excellence and as a National Center for Science Education, the PLM College of Science leads the academic
community for policy and decision – making that promotes intellectual excellence in undergraduate education. It aims
primarily to training bright Filipino high school students from Manila in overall scholarship, science education, and research.
The college encourages the faculty to be competitive in academic criteria, by adhering to international standards through its
multidisciplinary approach. This encompasses both teaching and research collaboration with foreign academicians in order
to enhance global competitiveness which will satisfy the demands of the 21st century.

C. Course Details

Course Name
Course Description
Number of units
Pre-requisite/s
Co-requisite/s
[CMB 3101L] CELL AND MOLECULAR BIOLOGY Laboratory
The course covers experimentations that would be possible to observe the cell and
its components with a special emphasis on the step-by-step process of each
methodology
2 units Contact Hour/s per week 6
CMB 3101

D. Course Outcome and Relationship to Program Outcome

Course Outcomes Program Outcomes

At the end of the course, the students should be able to: A B C D E F G H I J
Recognize novel molecular techniques used in various medical and scientific ✓ ✓ ✓ ✓
diagnostics
Apply fundamental principles of cell and molecular biology to analyze scientific ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓
journals of said subject matter.
Create scientifically-sound write-ups, theses and journal reviews which revolves ✓ ✓ ✓ ✓ ✓ ✓
on the field of molecular biology
Communicate to the layperson difficult topics and simplify it for easier ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓
understanding of the common people.
Design and construct functional projects and/or methodologies related to the ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓
topics covered
Develop desirable values such as patience, perseverance, self-discipline and ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓
honesty.

*Program Outcomes:
A. Practice appropriate skills in the analysis and organization of scientific data.
B. Demonstrate descriptive and experimental methods in interpreting technical problems.
C. Articulate and write biological concepts and information evidently and confidently with the scientific community.
D. Identify appropriate biology equipment and ably operate such tools in biology research.
E. Exhibit and uphold Filipino nationalism in the practice of profession.
F. Choose and illustrate suitable and applicable techniques in biology research.
G. Recognize and practice ethical and moral accountability.
H. Instill, appreciate and practice conservation measures to maintain a suitable environment.
I. Devise meaningful and relevant teaching styles to facilitate learning

6th Flr Gusaling Lacson, Intramuros, Manila; Tel / Fax No. 527-9075 [CMB 3101L] Cell and Molecular Biology Lab Page 1 of 3
 PAMANTASAN NG LUNGSOD NG MAYNILA
(University of the City of Manila)

COURSE SYLLABUS

E. Course Outline

Learning Outcomes Topics Learning Activities/ Assessment

Teaching Strategies
• To introduce the use of Introduction to Pipetting and • Video presentations/ Laboratory sheets
micropipettors, including Microvolume analysis Practical Exams
the careful use of such Dilutions • PowerPoint Recitations
equipment Presentations Presentations
• To learn how to accurately • Virtual Laboratories
dispense microvolumes of • Group Discussions
liquids
• To review basic serial
dilution techniques
• To recall the basic cellular Cellular Components and • Video presentations/ Laboratory sheets
processes and cell types the Hemocytometer analysis Practical Exams
• To reassess existing • PowerPoint Recitations
knowledge on the different Presentations Presentations
cell types and employ • Virtual Laboratories
techniques in viewing • Group Discussions
them
• To enrich the existing Lipid Solubility of • Video presentations/ Laboratory sheets
knowledge of the lipid Membranes analysis Practical Exams
component of the cell • PowerPoint Recitations
membrane Presentations Presentations
• To be able to relate the • Virtual Laboratories
effects of various chemicals • Group Discussions
onto the cell membrane
surface
• To be able to identify the Large Organelle Isolation • Video presentations/ Laboratory sheets
proper steps in the And Purification analysis Practical Exams
isolation and purification • PowerPoint Recitations
of large organelles such as Presentations Presentations
mitochondria and • Virtual Laboratories
chloroplasts • Group Discussions
• To recognize how
structure and size affects
the manner of isolation
from the cell suspension.
• To familiarize oneself on Bacterial Transformation • Video presentations/ Laboratory sheets
the complex process of analysis Practical Exams
bacterial transformation • PowerPoint Recitations
and its practical Presentations Presentations
applications on research. • Virtual Laboratories
• To identify the functions • Group Discussions
of each reagent within the
mixture used for bacterial
transformation
M I D T E R M E X A M I N A T I O N S
• To be able to design Restriction Digests, Primer • Video presentations/ Laboratory sheets
specific primers for a Design and the PCR analysis Practical Exams
specific DNA sequence • PowerPoint Recitations
• To identify the functions Presentations Presentations
of each reagent in the PCR • Virtual Laboratories
mix/ master mix during • Journal critique
PCR
• To differentiate the
phenomena happening on
each of the stages of PCR
• To familiarize oneself on Gel Electrophoresis • Video presentations/ Laboratory sheets
the use of electrophoresis analysis Practical Exams
on the visualization of • PowerPoint Recitations
target molecules Presentations Presentations
• To be able to know how to • Virtual Laboratories
troubleshoot various • Journal critique
problems arising during or
after a gel electrophoresis
run

6th Flr Gusaling Lacson, Intramuros, Manila; Tel / Fax No. 527-9075 [CMB 3101L] Cell and Molecular Biology Lab Page 2 of 3
 PAMANTASAN NG LUNGSOD NG MAYNILA
(University of the City of Manila)

COURSE SYLLABUS
• To familiarize oneself on DNA Quantitation • Video presentations/ Laboratory sheets
the use of a analysis Practical Exams
spectrophotometer in • PowerPoint Recitations
measuring the purity of a Presentations Presentations
DNA sample • Virtual Laboratories
• To be able to apply prior • Journal critique
knowledge on dilutions
onto a micro scale
• To be able to grasp the The Basics of ELISA • Video presentations/ Laboratory sheets
basics on performing analysis Practical Exams
ELISA and its applications • PowerPoint Recitations
• To be able to Presentations Presentations
troubleshoot various • Virtual Laboratories
errors arising from • Journal critique
improper ELISA
experimentations
F I N A L E X A M I N A T I O N S

F. Learning Resources
TEXTBOOKS AND REFERENCES:
Karp, Iwasa, Marshall (2015) Karp's Cell and Molecular Biology: Concepts and Experiments, 8th Edition
Alberts et al. (2002) Molecular Biology of the Cell
Brock et al. (2015) Biology of Microorganisms
Campbell et al. (2014) Campbell Biology
Karp et al. (2014) Essential Cell Biology

G. Computation of Grades

Class standing:
Lab sheets and Reports 20%
Practical Exams 30%
Modules, Essays, Asynchronous activities 10%

Major Examinations:
Midterm examination 20%
Final examination 20%

6th Flr Gusaling Lacson, Intramuros, Manila; Tel / Fax No. 527-9075 [CMB 3101L] Cell and Molecular Biology Lab Page 3 of 3