Republic of the Philippines

WESTERN MINDANAO STATE UNIVERSITY

College of Engineering
Zamboanga City

Engineering Graduate School

Master of Science in Environmental Engineering Program

Assignment 1
EnE 215 – Biochemical Engineering
First Semester SY 2022-23

Name: _______________________ Date: ________________

Course & Year: _________________ Score: _______________

Assignment Guidelines

1. Must be done by word processing or on computer.

2. Formatting:
 Font and font size. Arial 10.
 All paragraphs must be justified.
3. Proof-read your assignment before you submit for evaluation.

Topic 1

 None applicable

Topic 2

1. What is GMP and how does it relate to the regulatory process for pharmaceuticals?
2. When the FDA approves a process, it requires validation of the process. Explain what validation
means in the FDA context.
3. Why does the FDA approve the process and product together?

Topic 3

1. You wish to produce a high-value protein using recombinant DNA technology. Would you try to
develop a chemical defined medium or a complex medium? Why?
2. Contrast DNA and RNA. Cite at least four differences.
3. Contrast the advantages and disadvantages of chemically defined and complex media.

Topic 4

1. Consider the following reaction sequence:

k1 k3
S+ E ⇌ (ES)1 ⇌ (ES)2 k 5 P+ E
→
k2 k4

Develop a suitable rate expression for production formation [v = k 5( ES)2 ] by using

(a) the equilibrium approach, and
(b) the quasi-steady-state approach.