Ivan V.

Strekalovskiy
BIOTECHNOLOGIST, EMBRYOLOGIST

Work experience

08.2020 – currently Head of upstream process

ABOUT
development unit
ME
Date of Birth 04.05.1989
Bioprocess Development Laboratory, Biotech Division, Solopharm Marital status single
(Grotex LLC), Saint-Petersburg
Nationality Russian
- Heading all the research activities in the field of upstream development (cell
banks establishment and characterization, culture conditions optimization CONTACT
in 2L bioreactors) for all biosimilar projects in Grotex pipeline (5 monoclonals S
Email:
up to date) i.v.strekalovskiy@gmail.com
- Technology scale-up and transfer to pilot (50-200L) and production (1000L)
Phone: +7 950 005-84-99
scale at a GMP-compliant facility
Address: Energetikov 4 k.3,
- Carrying out proper information exchange with EU collaborators (ExcellGene
apt. 181, Saint Petersburg,
SA, Switzerland): interpreting meetings and conf-calls, facilitating written Russia
communication, data handling and representation etc.
- Development of technical documentation SKILLS
• Extensive experience in
mammalian cell culture
(upstream) process
09.2017 – 08.2020 Senior researcher development and
optimization;
Laboratory of cell therapy products development, BIOCAD, Saint-
• Human pluripotent stem
Petersburg
cells culture;
- Development of CAR-T cell therapy products • Human T-cell, NK-cell
- Lentiviral vector production using HEK293T expression platform, functional culture, immune cell-
LVp-titer assessment using model cell lines (SupT1, HEK293, HeLa) based cell therapy
- Cytotoxicity studies on CAR-positive T-cells development.
• Strong knowledge and
experience in general
lab workflow and
09.2016 – 09.2017 Researcher chemical analytical
methods and
Laboratory of cell line development, BIOCAD, Saint-Petersburg procedures (from pH-
- Development of stable producing cell lines for monoclonal antibody meters to more
production; sophisticated analyzers)
- Genetic engineering of stable producing CHO cell lines (co-expression of • Basic molecular biology
favourable allogenic regulatory genes, siRNA-mediated knock-down studies) (DNA, RNA);
• Basic ELISA and
proteomics;
• Extensive experience in
cell culture;
• Certain PC user
(Windows, Linux, Mac);
 11.2014 – 09.2016 Junior Researcher
SOFT SKILLS

Biotechnological processes development division, BIOCAD, Saint- • Problem solving

Petersburg • Adaptability
• Collaboration
- Scale-up studies for monoclonal antibody development using CHO cell
culture in benchtop bioreactors (0,5 and 2 L) with comprehensive DOE • Strong work ethic
approach using statistical analysis software (MODDE, SIMCA) • Time management
- Cell culture strategy optimization for upstream process, including screenings • Critical thinking
of different types of media and feedings, culture conditions and equipment;
- Biochemical analysis of conditional cell culture media using state-of-art LANGUAGES
equipment (NOVA Flex, Roche CedexBio). Russian
Native

English
05.2012 – 11.2014 Junior Researcher Fluent

Laboratory of General Toxicology and Hygienic Regulation, Research HOBBIES

Institute of Hygiene, Occupational Pathology and Human Ecology
(RIHOPHE), Saint-Petersburg
Sports
- Rodents (SPF rats and mice) colony maintaining, whole scope of a rodent
vivarium caretaking work; I have been playing football
- Standard toxicological in vivo studies on animals with intravenous, for amateur and corporate
intraperitoneal introduction, etc teams my whole life, also a
- Developing an integrated test-system for assessment of cyto-, geno- and vivid fitness addict and
embryotoxicity based on rat pre- and postimplantation embryo culture; hiker.
- In vitro testing of a wide range of toxic effects on rat and human blood
samples and cell lines;
- Obtaining, passaging and targeted differentiation of mouse embryonic stem Traveling
cells and other mammalian primary cell cultures;
- Micromanipulation and microsurgery of embryos, microinjection of stem I have visited more than 20
cells and soluble agents; countries.
- Wide range of analytical methods including microscopy, immunochemical,
histological, spectrophotometric methods and SDS-PAGE
Music

I play guitar, dig for rock

music concerts and have a
09.2009 – 05.2012 Lab Assistant
vinyl records collection.
Department of Embryology, Faculty of Biology and Soil Sciences, Saint-
Petersburg State University, Saint-Petersburg
- Caretraking of a Platynereis dumerilii culture (at Roman P. Kostyuchenko lab) Pets
and a cnidarians aquatic culture (Bougainvillia superciliaris, Rathkea
I keep an aquatic pet — a
octopunctata).
mexican axolotl — already
- Seasonal, on the premises of SPBSU White sea Marine Biological station:
for 11 years and he feels
gametes preparation, artificial fertilization for embryo culture of Alitta virens
absolutely fine!
and Testudinalia tesselata;
- Managing and caretaking of axolotl (Ambystoma mexicanum) collection (at
Yegor B. Malashichev lab), incl. breeding and intranuclear DNA
microinjection for transgenesis;
- Maintaining of a Danio rerio fishtank and caretaking of the culture;
 Education and scientific experience
09.2010 – 06.2012 Master’s Degree in Cell and Molecular Biology and
Biotechnology, GPA 4.7/5

09.2006 – 06.2010 Bachelor's Degree in Biology, GPA 4.7/5

Yegor B. Malashichev scientific group of experimental embryology of

vertebrates
Research project: “Influence of intermediate mesoderm on the development
of proximal elements of the limb girdles in domestic chick (Gallus domesticus)”

Department of Embryology, Faculty of Biology and Soil Sciences, Saint-

Petersburg State University, Saint-Petersburg

Additional courses

05.2013 Advanced postgraduate course “Cell therapy products —

main types, principles of action, manufacturing organization and
GMP”, 108 hours

Institute of Postgraduate Professional Education (A. I. Burnazyan Federal

Medical-Biological Centre, Moscow, Russia)

04.2018 Advanced postgraduate course “Flow Cytometry Technique

in the medical and biological research”; 72 hours

Saint-Petersburg State University Research Park

Publications:

1. Strekalovskiy IV, Shabasheva LV, Popov VB, Panfyorova YA Express

assessment of geno-, cyto- and embryotoxic effects of potential teratogens
using an integrated in vitro test-system based on rat whole embryo culture //
ALTEX Proceedings Vol. 3, No. 1 (2014), 9th World Congress on Alternatives
and Animal Use in the Life Sciences, Prague, 24–28 Aug 2014. – P. 137.

2. Strekalovskiy IV, Shabasheva LV, Krylova TA, Golubentseva YV, Popov VB

[Application of cell technologies for correction of toxic lesions in rodents] //
Technologies of living systems. – 2013. – № 8. – P. 90–92, in Russian.

3. Babakhanova RA, Smirnova NP, Kondakova EA, Strekalovskiy IV, Kostyuchenko

RP [The impact of U0126 МАРК activation inhibitor on the development of a
mollusk Testudinalia tesselata] // Proceedings of the 11th Scientific session
of the Saint Petersburg state university marine biological station, Saint
Petersburg, 02 Feb 2010. – P. 56–57, in Russian.