User Manual - 161150-1293 - A - en - Masterclave 10-20

®
MASTERCLAVE 10
®
MASTERCLAVE 20
User Manual - Original

Instructions
161150-1293 - A - en - 2019-07 - 161150-1293
Industry Use - EN
Table of Contents
Introduction to the System.....................................................................................1-1

Intended Use and Users.............................................................................................. 1-1
Benefits and Limitations of Use................................................................................... 1-1
Warning and Safety Messages.................................................................................... 1-1
Statement Types............................................................................................... 1-1
General Statements..................................................................................................... 1-2
Standard Symbols........................................................................................................1-5
Safety Information...................................................................................................2-1
System Compliance..................................................................................................... 2-1
FCC Compliance...............................................................................................2-1
IEC Compliance................................................................................................ 2-1
Industry Canada (IC) Compliance.....................................................................2-2
Instrument Labels........................................................................................................ 2-2
Safety Precautions.......................................................................................................2-3
System Description and Basic Operations...........................................................3-1

System Description...................................................................................................... 3-1
Front View (MASTERCLAVE® 10)....................................................................3-1
Front View (MASTERCLAVE® 20)....................................................................3-2
Control Unit....................................................................................................... 3-3
Context-Sensitive Help......................................................................................3-3
The Lid.............................................................................................................. 3-4
The Vessel (MASTERCLAVE® 10)................................................................... 3-5
The Vessel (MASTERCLAVE® 20)................................................................... 3-5
Rear View (MASTERCLAVE® 10).................................................................... 3-6
Rear View (MASTERCLAVE® 20).................................................................... 3-7
Left Side View................................................................................................... 3-8
Right Side View.................................................................................................3-9
Light Indicators................................................................................................3-10
Alarm Systems................................................................................................ 3-11
Reagents....................................................................................................................3-11
List of Accessories..................................................................................................... 3-11
List of Consumables.................................................................................................. 3-12
Technical Data and Specifications............................................................................. 3-12
Technical Data and Specifications - MASTERCLAVE 10.............................. 3-12
Technical Data and Specifications - MASTERCLAVE 20.............................. 3-13
System Basics........................................................................................................... 3-15
Overview of Operation Elements.................................................................... 3-15
System Installation and Configuration................................................................. 4-1

Unpacking the Instrument............................................................................................ 4-1
Installing the Instrument...............................................................................................4-2
Connecting the MASTERCLAVE® 10 to the Power Supply Network...........................4-3
Connecting the MASTERCLAVE® 10 to the Single-Phase Power Network..... 4-3
Connecting the MASTERCLAVE® 10 to the Three-Phase Power Network
without a Neutral Power.................................................................................... 4-4
Connecting the MASTERCLAVE® 20 to the Power Supply Network...........................4-6
Connecting the MASTERCLAVE® 20 to the Three-Phase + Neutral Power
Network............................................................................................................. 4-6
Connecting the MASTERCLAVE® 20 to the Three-Phase Power Network
with no Neutral Power....................................................................................... 4-7
161150-1293 - A i MASTERCLAVE® 10 MASTERCLAVE® 20

Table of Contents
Connecting the MASTERCLAVE® 20 to a Demineralized Water Source ................... 4-9

Connecting the MASTERCLAVE® 20 to a Demineralized Water Tank............. 4-9
Installing the Auto Water Filling for Tank for the MASTERCLAVE® 20.............4-9
Connecting the MASTERCLAVE® 20 to a Demineralized Water Supply
Network .......................................................................................................... 4-10
Installing the Auto Water Filling for Network for the MASTERCLAVE® 20..... 4-11
Starting the MASTERCLAVE®...................................................................................4-12
User Management..................................................................................................... 4-12
Activating/Deactivating the Users Option........................................................4-12
User Access Rights.........................................................................................4-13
Creating a User Account................................................................................. 4-15
Modifying a User Account............................................................................... 4-16
Deleting a User Account................................................................................. 4-16
Configuring the User Account Settings........................................................... 4-16
Changing a User Session............................................................................... 4-16
Managing My User Account............................................................................ 4-16
Configuring the Software Settings............................................................................. 4-17
Exporting the Configuration Settings for the Instrument................................. 4-17
Importing the Configuration Settings...............................................................4-17
Configuring the Localization Settings..............................................................4-18
Adjusting the Energy Settings for the Equipment............................................4-18
Configuring the Current Date, Time, and Time Zone...................................... 4-18
Enabling/Disabling Sounds............................................................................. 4-18
Updating the Firmware...............................................................................................4-19
Workflow and Instructional Procedures............................................................... 5-1

Starting the System .....................................................................................................5-1
Shutting Down the System ..........................................................................................5-1
Managing Programs.....................................................................................................5-1
Activating/Deactivating the Programs Management......................................... 5-1
Creating a Simple Program ..............................................................................5-1
Creating a Program for Supplement Addition at a High Temperature...............5-2
Creating a Program to Add Supplements at a Dispensing Temperature ......... 5-2
Deleting a Program........................................................................................... 5-3
Filling the Water Bath...................................................................................................5-3
Filling the Water Bath Manually ....................................................................... 5-3
Using the Automatic Water Bath Filling System (MASTERCLAVE® 20).......... 5-3
Performing Cycles .......................................................................................................5-3
Performing a Cycle (Without Program Management)....................................... 5-3
Performing a Cycle (With Program Management)............................................ 5-4
Performing a Delayed Start Cycle.....................................................................5-5
Pressurizing the Instrument......................................................................................... 5-6
Dispensing with an e-pump..........................................................................................5-7
Displaying and Exporting Traceability Information.......................................................5-7
Managing the Peripherals............................................................................................ 5-8
Activating/Deactivating the Embedded FTP Server.......................................... 5-8
Enabling/Disabling the i™ Volume.................................................................... 5-8
Connecting a USB Stick....................................................................................5-8
Configuring a Wifi Stick..................................................................................... 5-9
Configuring the Ethernet................................................................................... 5-9
Configuring a Remote FTP Server....................................................................5-9
Connecting a Physical Printer......................................................................... 5-10
Creating a Virtual Printer.................................................................................5-10
Changing a Label Model................................................................................. 5-10
Importing a Label Model..................................................................................5-11
Configuring a Barcode Reader........................................................................5-11
Configuring the Communication Settings for the LIMS.............................................. 5-11
Configuring E-Mail Alerts........................................................................................... 5-12
161150-1293 - A ii MASTERCLAVE® 10 MASTERCLAVE® 20

Table of Contents
Managing Messages..................................................................................................5-12
Reading a Message........................................................................................ 5-12
Deleting a Message........................................................................................ 5-12
Consulting the Instrument Information.................................................................6-1
User Maintenance................................................................................................... 7-1

Calibration and Adjustments........................................................................................ 7-1
Calibrating the Culture-Medium Vessel Sensor ............................................... 7-1
Adjusting the Culture-Medium Vessel Sensor ..................................................7-1
Calibrating the Demineralized Water Flow Meter..............................................7-1
Adjusting the Demineralized Water Flow Meter ............................................... 7-2
Printing a Certificate..........................................................................................7-2
Restore the Calibration and Adjustment Certificates........................................ 7-2
Defining the Maximum Permissible Error (MPE)...............................................7-3
Defining the Validity Period for Metrology Certificate........................................7-3
Cleaning Procedures................................................................................................... 7-3
Cleaning the Instrument ................................................................................... 7-3
Performing a Cleaning Cycle............................................................................ 7-3
Cleaning the Non-Return Valve........................................................................ 7-4
Cleaning the Automatic Filling Kit..................................................................... 7-4
Cleaning the Auto Water Filling for Tank (MASTERCLAVE® 20)......................7-4
Cleaning the Auto Water Filling for Network (MASTERCLAVE® 20)................ 7-5
Preventive Maintenance Operations............................................................................7-7
Replacing Seals and Filters.............................................................................. 7-7
Draining the Water Bath....................................................................................7-7
Replacing the Water Inlet and Outlet Hoses..................................................... 7-8
Checking Sensor State..................................................................................... 7-8
Fitting a Roll of Paper in the Printer............................................................................. 7-8
Managing the Maintenance Logs.................................................................................7-9
Authorizing Remote Control.........................................................................................7-9
Performing Functional Tests........................................................................................ 7-9
Appendix - Troubleshooting..................................................................................A-1
Error Messages and Recovery Procedures.................................................................A-1
Appendix - Third-Party Software...........................................................................B-1
Appendix - Glossary...............................................................................................C-1
Adjustment.................................................................................................................. C-1
Calibration................................................................................................................... C-1
F0................................................................................................................................ C-1
LIMS............................................................................................................................ C-1
Revision History
161150-1293 - A iii MASTERCLAVE® 10 MASTERCLAVE® 20

General Information
The content of this document is based on the Software release 1.0.

Please discard any previous copies of this document if relevant.
This document may contain information or references relating to certain bioMérieux products,
software or services which are not available in the country of release; this shall not mean that
bioMérieux intends to market such products, software or services in such country.
To request copies of publications or for any technical request / assistance, contact bioMérieux
or your local distributor (contact information available on www.biomerieux.com).
Note: The screens and figures shown are intended as illustrations only and must not be interpreted
as actual representations of data, results or equipment.
Screens and equipment are not shown to scale.
IMPORTANT: Please read this document carefully before using the system.
Limited Warranty
bioMérieux warrants the performance of the product for its stated intended use provided that
all procedures for usage, storage and handling, shelf life (when applicable), and precautions
are strictly followed as detailed in the instructions for use (IFU).
Except as expressly set forth above, bioMérieux hereby disclaims all warranties, including any
implied warranties of merchantability and fitness for a particular purpose or use, and disclaims
all liability, whether direct, indirect or consequential, for any use of the reagent, software,
instrument and disposables (the "System") other than as set forth in the IFU.
Customer acknowledges and agrees that use of the System for testing of sample types or for
indications other than those described in the IFU is done solely at the Customer’s own risk.
Customer acknowledges and agrees that it is Customer’s sole and exclusive responsibility to
validate the System for any such intended use, and to determine whether the System is
suitable for that intended use. The performance of any validation studies and the subsequent
use of the System based on Customer’s validation studies shall be the Customer’s sole risk
and responsibility.
Product warranty details can be obtained from bioMérieux or your local distributor (contact
information available on www.biomerieux.com).
Intellectual Property
BIOMERIEUX, the BIOMERIEUX logo, MASTERCLAVE, PMI and DILUMAT are used,
pending, and/or registered trademarks belonging to bioMérieux, or one of its subsidiaries, or
one of its companies.
Any other name or trademark is the property of its respective owner.
© 2019 bioMérieux SA
bioMérieux SA 673 620 399 RCS Lyon
1 Introduction to the System
Intended Use and Users
The MASTERCLAVE® is used for automated preparation of culture media, agars, or broths,
and is to be used for microbiological tests in the clinical, pharmaceutical, food processing, and
cosmetics industries.
This equipment is to be used by qualified, trained laboratory professionals in the
aforementioned industries.
Benefits and Limitations of Use
MASTERCLAVE® optimizes the workflow through lab automation. It enhances the following:
• Productivity by removing the preparatory stages such as medium stirring and bottle
autoclaving
• Reproducibility by preventing human error and ensures batch-to-batch reproducibility.
• Flexibility as it is suitable for both agar and broth preparation.
• Easy to use by adding powder and distilled water to implement a cycle. No supervision
required during the cycle.
The MASTERCLAVE® must be used only for the purposes specified in the instructions for
use.
Warning and Safety Messages
The user documentation uses several types of statements to alert you to important
information. Important information is labeled in text and identified using symbols.
Statement Types
The statement types are Warning, Caution, Important, and Note. The following examples
define each statement type. The general caution symbol is used in these examples, but other
symbols (see Standard Symbols) may be used instead.
The warning messages in this document mainly refer to:
WARNING
A Warning statement alerts the user to the possibility of injury, death, or
other serious adverse reactions associated with the use or misuse of the
device.
161150-1293 - A 1-1 MASTERCLAVE® 10 MASTERCLAVE® 20

General Statements Introduction to the System
CAUTION: A Caution statement alerts the user to the possibility of a problem

with the device associated with its use or misuse. Such problems include
device malfunction, device failure, damage to the device, or damage to other
property. Where applicable, a Caution statement may include a precaution
that should be taken to avoid the hazard.
IMPORTANT: An Important statement relates to content presented in the user documentation. It is

used to reinforce the user’s understanding of selected information.
Note: A Note statement supplies additional information about a topic.
General Statements
This section provides important statements that apply to all products. Equipment meets the
requirements and standards stated in the certificate supplied with it.
WARNING
Equipment is intended for professional use only.
Laboratory personnel should be qualified and adhere to the principles of
good laboratory practice.
All the user documents supplied must be read prior to use of the equipment.
Under no circumstance should the user dismantle equipment due to the risk
of touching dangerous parts, including parts that may be infectious or
connected to a source of electric power.
Do not obstruct the equipment and hardware ventilation apertures, and leave
sufficient clearance around the equipment for the circulation of air.
All biological materials should be considered as potentially infectious.
Suitable individual protective equipment is required when handling chemical
or biological substances.
bioMérieux is in no case liable for the harmful consequences of incorrect use
or improper handling of these substances.

WARNING
Electromagnetic Compatibility (EMC):
The EMC class of the equipment is indicated on the certificate supplied with
it.
If equipment is a class A product, it may cause radio interference in a
domestic environment, in which case the user will be required to correct the
interference at his own expense.
Do not use this device near strong sources of electromagnetic radiation (for
example, intentionally unprotected radio-electric sources), which could
interfere with the operation of the equipment.
It is recommended to evaluate the electromagnetic environment before
starting the device.
WARNING
In order to avoid computer viruses or abnormal functioning of your
equipment, never download any software other than those ensuring the
protection of your network and those provided or recommended by
bioMérieux.
It is your responsibility to secure your network and ensure this protection is
appropriate and maintained. It is recommended to use all appropriate means
(including antivirus software, security patches, firewall) to protect your
network from virus intrusion, unauthorized use, alteration, manipulation and
disclosure.
In an effort to reduce the risk of spreading a virus to bioMérieux equipment,
it is recommended that only bioMérieux supplied USB devices are used with
bioMérieux equipment. The use of personal USB devices is not
recommended. To avoid computer viruses and the potential loss of
functionality and/or results, use caution when transferring USB devices
between computers. Do not use USB devices intended for bioMérieux
equipment in other computers that do not have current antivirus software
installed and active.
All computer media (CD, DVD, USB key) supplied with this equipment should
be stored and stocked in a suitable location.
Only modify the software configuration parameters you are authorized to
modify and which are described in the user documentation.

WARNING
Decontamination of equipment at the end of its life cycle:
The following instructions must be followed by all users in countries where
local legislation imposes the treatment and recycling of equipment at the end
of its life cycle.
As a general rule, and as a precautionary measure, any part of the equipment
(including sub-assemblies, components and materials) considered to be
potentially infectious, must be decontaminated, whenever possible, or
removed if decontamination is impossible or presents a risk.
Any part considered to be potentially infectious, which is not
decontaminated, must be removed from the instrument before following the
normal channels for elimination of infectious products, in accordance with
local regulations.
The decontamination instructions in the user documentation correspond to
the parts of the equipment that are potentially infectious according to their
intended use. These operations must be performed before the equipment is
transferred to a third party.
However, bioMérieux cannot exclude that other parts of the equipment have
not been contaminated in other circumstances, in particular as the result of
spillage of infectious substances. In this case, the user is solely responsible
for decontaminating these parts or removing them before they follow the
normal channels for elimination of infectious products.
WARNING
This statement only applies to European countries with regard to the waste
electrical and electronic equipment European directive:
You can play an important role in contributing to reuse, recycling, and other
forms of recovery of waste electrical and electronic equipment. Sorting this
type of waste significantly reduces potential negative effects on the
environment and human health as a result of the presence of hazardous
substances in electrical and electronic equipment.
At the end of the life cycle of this product, do not dispose of the product as
unsorted municipal waste, even if it is decontaminated. It is imperative that
you contact bioMérieux to assure its appropriate disposal.
IMPORTANT: Electrical or other connections should only be made using the accessories supplied
with the equipment.
IMPORTANT: It is important to follow all the restrictions on use, particularly concerning

temperature, storage, installation and voltage, which are indicated on the product
label or in the user documentation.

Standard Symbols Introduction to the System
IMPORTANT: The accuracy of results obtained with this equipment depends on the maintenance
operations described in the user documentation (user maintenance and/or periodic
preventive maintenance performed by bioMérieux).
IMPORTANT: The user should be aware that if the maintenance operations are not performed, are
only partially performed, or are not performed as described in the user
documentation, bioMérieux is in no case liable for any false test results obtained.
IMPORTANT: It is recommended to keep the original packaging materials in case the equipment
needs to be moved. Any damage directly or indirectly resulting from the transport of
the equipment without adequate containers will not be covered by the warranty.
Standard Symbols
The following table defines symbols that may appear in the instructions for use or on the
instrument, package inserts, or packaging. When surrounded by a triangle on a yellow
background, the symbol highlights an immediate warning and is positioned on the instrument
itself.
CE-Marking of Conformity
Compliance with China RoHS

Regulation (Chinese Standard SJ/
T11364)
Eurasian Conformity
Batch code Catalogue number
Serial number Consult Instructions for Use
Use by date Manufacturer
Date of manufacture This way up
Caution, consult accompanying

Do not stack
documents
Contains sufficient for <n> tests Do not reuse
Keep dry Keep away from light

Fragile, handle with care Humidity limitation
Keep away from magnetic field Temperature limitations
Upper limit of temperature Lower limit of temperature
Sterile Positive control
Negative control Biological risk
Electric shock warning Radiation warning
Hot surface Potential pinch-point warning
Laser beam Shearing hazard
High temperature Hazardous magnetic field
Potential tip-over/crush hazard Acute toxicity
Corrosives Sodium azide

Irritant Recyclable
Environmentally friendly use period.

Separate collection for waste electrical Actual number of years may vary by
and electronic equipment product. This symbol is typically
orange in color.
Fuse Direct current
Alternating current Both direct and alternating current
Three-phase alternating current Earth (ground) terminal
Protective conductor terminal Frame or chassis terminal
Equipotentiality ON (supply)
ON (only for a component of the

OFF (supply)
system equipment)
Equipment protected throughout by

OFF (only for a component of the double insulation or reinforced
system equipment) insulation (Equivalent to Class II of IEC
536)
Ethernet port

2 Safety Information
It is essential that the warnings, cautions and safety requirements contained in this document
are read and understood by the user before operating the system.
Warning symbols have been placed on the system to draw your attention to areas of potential
hazards.
System Compliance
FCC Compliance
This device complies with Part 15 of the FCC Rules. Operation is subject to the following two
conditions:
1. This device may not cause harmful interference, and
2. This device must accept any interference received, including interference that may
cause undesired operation.
This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide
reasonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio frequency
energy and, if not installed and used in accordance with the instruction manual, may cause
harmful interference to radio communications. Operation of this equipment in a residential
area is likely to cause harmful interference in which case the user will be required to correct
the interference at his own expense.
Modifications: Any changes or modifications made to this equipment that are not approved by
BIOMERIEUX SA FRANCE may cause harmful interference and void the FCC authorization
to operate this equipment.
This equipment is compliant with the limits stipulated by the FCC regarding exposure to
radiation within an open access environment:
• This equipment must be installed and operated at a minimum distance of 20 cm from the
user and from any surrounding people.
• This emitter must not be placed next to or operated at the same time as another antenna
or emitter.
IEC Compliance
This instrument complies with the emissions and immunity requirements of IEC 61326.
This is a Class A product. The instrument was designed and tested against CISPR 11, class
A. In a domestic environment this product may cause radio interference, in which case the
user may be required to take adequate measures.
bioMérieux recommends the evaluation of the electromagnetic environment before operating
the instrument.
CAUTION: Use of this instrument in a dry environment, especially if synthetic

materials are present (for example synthetic clothing and carpets) may cause
damaging static discharges that may cause erroneous results.

Industry Canada (IC) Compliance Safety Information
Industry Canada (IC) Compliance
Industry Canada (IC) Compliance

This device complies with Industry Canada license-exempt RSS standard(s). Operation is
subject to the following two conditions:
1. This device may not cause interference, and
2. This device must accept any interference, including interference that may cause
undesired operation of the device.
CAN ICES-3(A) / NMB-3 (A)
Instrument Labels
Note: The picture of the rating plate is provided for information purposes only. The marks of
conformity printed on it depend on the current regulations in each country in which the
instrument is marketed.
MASTERCLAVE® 10
MASTERCLAVE® 20

Safety Precautions Safety Information
1 Danger - Steam release

2 Danger - Hot surface
3 Name Plate This device complies with
Part 15 of the FCC Rules.
Operation is subject to the
following two conditions:
1. this device may not
cause harmful
interference, and
2. this device must accept
any interference
received, including
interference that may
cause undesired
operation.
Safety Precautions
Pay particular attention to the following safety precautions. If these safety precautions are
ignored, injury to the operator or damage to the instrument may occur. Each individual
precaution is important.
WARNING
If the equipment is used in a manner not specified by the manufacturer, the
protection provided by the equipment may be impaired.

WARNING
All biological samples and quality control (QC) products incubated in this
system, as well as all waste in the waste containers, should be treated as
potentially biohazardous materials. All materials and mechanical
components associated with the waste systems should be handled
according to safe microbiological practices in compliance with the
installation site's biohazard procedures. Use the personal protective
equipment recommended by the facility when handling any of these
components, including gloves, safety glasses, and a lab coat.
The system must be decontaminated before its covers are removed by
service personnel. Ensure that appropriate decontamination is carried out if
hazardous materials are spilled on or into the equipment or surrounding
areas.
WARNING
Treat waste material, including consumable items, and any components
coming into contact with waste material as having the potential hazards of
the samples used.
All service personnel should be familiar with the Material Safety Data Sheet
(MSDS) for all materials used in the procedures relating to this instrument,
and the correct procedures for handling these materials.
WARNING
Even when power is removed from the instrument, the potential exists for
electricity to be generated if components, such as assemblies that are
mounted on belts, are moved too quickly. Components should be moved
slowly to prevent the buildup of electricity. Failure to comply may result in
injury to personnel or damage to the instrument.
WARNING
Do not replace detachable main supply cords by inadequately rated cords.
Only use main supply cords provided by the manufacturer.
Do not replace electric cables. If cables do not have the same technical
specificities, there is a risk of electrical shock.
WARNING
Electronic equipment can be the source of electrical shocks. Installation,
service, and repair should only be performed by authorized and qualified
bioMérieux personnel.

WARNING
All power switches should be off when connecting or disconnecting cables
to power outlets to reduce the risk of electrical shock.
WARNING
bioMérieux recommends connecting this instrument to a main power outlet
that is protected with a ground fault circuit interrupter to reduce the risk of
electrical shock.
WARNING
As with any mechanical system, certain precautions must be taken when
operating the instrument. The instrument has a protective cover intended to
prevent the operator from coming into contact with any moving parts and
aerosols. When servicing the instrument, take special care, as there are
moving parts that can cause injury.
WARNING
Cleaning and disinfecting solutions have corrosive properties. Always wear
protective (chemical resistant) gloves and safety glasses when handling
cleaning and disinfecting solutions.
WARNING
Hot surfaces can cause injury.
DANGER
Do not open the MASTERCLAVE® when the pressure gauge does not
indiquate 0. Wait for the vessel to cool down before opening. In case of
problem, contact bioMérieux or your local bioMérieux representative.
DANGER
Do not open the safety valve manually when the pressure gauge does not
indiquate 0. Wait for the vessel to cool down before opening. In case of
problem, contact bioMérieux or your local bioMérieux representative.

CAUTION: Any liquid spilled on the system may result in system

malfunctioning. If liquid is spilled on the system, wipe it up immediately
using decontamination wipes.
CAUTION: The computer and its operating system have been carefully
configured for optimal system performance. Altering the configuration may
severely hamper the usability of the instrument.
Note: Before performing electrical safety or other compliance testing on the instrument, contact
bioMérieux or your local distributor.
WARNING
The user must only perform the maintenance operations described in this
document and rigorously follow each of the steps.
The use of tools not specified by bioMérieux is forbidden.
Powder-less gloves, a lab coat and protective glasses or goggles must be
worn when performing maintenance operations.
Always use personal protective equipment, including gloves, a lab coat and
safety glasses or goggles when handling reagents.
CAUTION: Take extra care when moving the instrument to avoid injury.
Before moving the instrument, be careful to remove any mobile objects
(beakers, e-pump, etc.)

3 System Description and Basic Operations
System Description
Front View (MASTERCLAVE® 10)
1 Safety cover
2 Cover assistance system
3 Light indicator
4 Control unit
5 On/off switch
6 Pressure gauge
7 Cover lock
8 Lid

Front View (MASTERCLAVE® 20) System Description and Basic Operations
Front View (MASTERCLAVE® 20)
1 Safety cover
2 Cover assistance system
3 Light indicator
4 Control unit
5 On/off switch
6 Pressure gauge
7 Cover lock
8 Lid

Control Unit System Description and Basic Operations
Control Unit
1 Touch screen
2 Menu keys
Context-Sensitive Help
The context-sensitive help system included in the panel gives direct access to information on
the instrument features.
1. Press , no matter which screen you are working on.
2. Press on the part of the screen with which you need help.
3. Read the information.

The Lid System Description and Basic Operations
The Lid
1 Lid securing catch (x5)

2 Lid handles
3 Priming port cap
4 Port cap for supplement addition
5 Air inlet filter
6 Air filter non-return valve
7 Lid seal
8 Priming tube connector
9 Priming tube

The Vessel (MASTERCLAVE® 10) System Description and Basic Operations
10 Media temperature probe

11 Optional external temperature probe
The Vessel (MASTERCLAVE® 10)
1 Removable stirrer
2 Culture medium vessel handle
The Vessel (MASTERCLAVE® 20)
1 Temperature probe and priming tube holder

Rear View (MASTERCLAVE® 10) System Description and Basic Operations
2 Removable stirrer
3 Culture medium vessel handle
Rear View (MASTERCLAVE® 10)
1 Air inlet tube hole

2 Compressed air outlet
3 Safety valve
DANGER
Never activate the safety valve when the vessel is pressurized.
4 Water inlet
5 Water outlet
6 Power supply cable
7 Peripherals connection panel
8 Lid assistance system

Rear View (MASTERCLAVE® 20) System Description and Basic Operations
Rear View (MASTERCLAVE® 20)
1 Air inlet tube hole

2 Compressed air outlet
3 Safety valve
DANGER
Never activate the safety valve when the vessel is pressurized.
4 Demineralized water inlet

5 Water inlet
6 Water outlet
7 Power supply cable
8 Peripherals connection panel
9 Lid assistance system

Left Side View System Description and Basic Operations
Left Side View

MASTERCLAVE® 10
MASTERCLAVE® 20
1 Cover lock
2 Demineralized water supply connection
3 Water supply connection

Right Side View System Description and Basic Operations
Right Side View

MASTERCLAVE® 10
MASTERCLAVE® 20
1 Optional ticket printer

2 USB port

Light Indicators System Description and Basic Operations
Light Indicators
Different light indicators keep the user informed on the state of the equipment.
1 I light indicator
Indicator front view
I light Color Explanation

indicator
blue • equipment on
• equipment ready to operate
blue (rapid • cycle in progress

blinking)
blue (slow • waiting for deferred programed cycle start

blinking)
green (blinking) • operation completed
red • operation failed

• an error has occurred
orange • deferred start initialization

(blinking)
• waiting for user response (ex: message confirmation)

Alarm Systems System Description and Basic Operations
I light Color Explanation

indicator
colorless • equipment off
white (blinking) • equipment in sleep mode
pink (blinking) • response to dispensing device
Alarm Systems
Three types of alarms provide information on cycles in progress.
•
Visual alarms: The Light Indicator is located at the front of the instrument and displays
different colors for different alarms.

• Sound alarms: These alarms emit beeps of varying lengths according to the type of alarm.
• Software alarms: These alarms are messages displayed on the device screen.
Reagents
To get ordering information, contact bioMérieux or your local distributor (contact information
available on www.biomerieux.com).
List of Accessories
• M10 mobility kit (includes trolley and rapid coupler connectors)
• M20 mobility kit (includes trolley and rapid coupler connectors)
• Built-in ticket printer (includes thermal printer and 20 paper rolls)
• Masterclave dosing kit (includes e-pump + extension cord PMi Tubing set 3.2mm with
nozzles for M10-M20 and footswitch)
• Auto water filling for network (M20)
• Auto water filling for tank (M20)
• Adding port cap with septum
• Water gun
• 1D barcode reader (USB)
• 2D barcode reader (USB)
• WIFI adapter
• Power cord filling 230V EC or w/o plug (Only for countries with 220V-230V [one phase] -
Power supply: to be used in dispensing phase only)
• Rapid coupler connectors (2U)
• Footswitch
• E-pump
• IQ/OQ

List of Consumables System Description and Basic Operations
• Media temperature probe M10 V2 calibrated

• Media temperature probe M10 V2
• Media temperature probe M20 V2 calibrated
• Media temperature probe M20 V2
List of Consumables
• M10-M20 consumables package (includes priming port cap seals, adding port cap seals,
priming nozzle seals set, non-return valve seals, priming flexible tubing for M10 and M20,
decompression filters 0.22µm, stirrer axe black washers & black gaskets)
• PMi tubing set 3.2 mm for M10-M20
• Dilumat tubing set for M10-M20
• Dilumat tubing set dual mode M10-M20
• Masterclave priming nozzle
• Draining cap
• Air inlet non-return valve
• MASTERCLAVE® printer paper rolls
• Water inlet filter 12-20
• Stainless steel hose clamp
• Suction grid
• MASTERCLAVE® 10 Medium vessel
• MASTERCLAVE® 20 Medium vessel
• Stirrer
• Cleaning brush
• Septums (50U)
Technical Data and Specifications
Technical Data and Specifications - MASTERCLAVE 10
Environmental Maximum altitude up to 2,000 meters

conditions
Ambient temperature 10 to 40°C max.
Relative humidity (RH) 30 to 80% without condensation
Atmospheric pressure 700 hPa to 1100 hPa
Power supply voltage • 230V ~ ± 10% single phase, 16A 50 Hz
• 200-210V ~ ± 10% three-phase + with no
neutral, 16A 60 Hz
Power consumption • 230V power supply: 3,300 Watts ± 10%

• 200-210V power supply: 2,800 Watts ± 10%

Technical Data and Specifications - MASTERCLAVE 20 System Description and Basic Operations
Water supply Minimum flow rate 5L/min

Minimum temperature 5°C
Maximum temperature 20°C
Hardness 0.7 to 2 mmol/L (CaCO3) = 7 to 20°Fh (TH)
Minimum pressure 1 bar
Maximum pressure 4 bar
• If the pressure is above the maximum recommended threshold,install a

pressure regulator valve
• If the pressure is below the minimum recommended threshold, install a
pressure booster pump.
Vessel working pressure 0 to 1.6 bar

Vessel design pressure 1.7 bar
Production capacity 10 liters max. (medium + demineralized water +
additional elements)
Installation category 2 (according to European standard ECI664)
Overvoltage category Category II
Pollution degree Category 2
Sound level < 70 db
Dimensions Instrument alone 550 x 440 x 650 mm (cover closed)
(W x H x D) Instrument (packed) 1,200 x 720 x 800 mm
Weight Instrument alone 85 kg

Instrument (packed) 105 kg
Temperature accuracy ± 0.5°C in stabilized phase
TFT resistive touchscreen Size: 15.24 x 9.14 cm (7 inches)
Resolution: 800 x 480 px
Keypad 8 physical keys

Fuse 16A 10 x 38 mm T 500 VAC (for serial number
inferior or equal to 10089048 or serial numbers
starting with 5 et 6)
All-pole circuit breaker All-pole circuit breaker from serial number superior
to 10089048 and not starting with 5 or 6
Lithium battery • Panasonic CR2032 - Nominal charge: 3V
225 mAh - Continuous standard charge: 0.2 mA
• Renata CR2032 - Nominal charge: 3V 235 mAh
- Continuous standard charge: 0.2 mA
Technical Data and Specifications - MASTERCLAVE 20

Technical Data and Specifications - MASTERCLAVE 20 System Description and Basic Operations
Environmental Maximum altitude up to 2,000 meters

conditions
Ambient temperature 10 to 40°C max.
Relative humidity (RH) 30 to 80% without condensation
Atmospheric pressure 700 hPa to 1100 hPa
Power supply voltage • 230/400V ~ ± 10% three-phase + neutral,
16A 50 Hz
• 200-210V ~ ± 10% three-phase with no
neutral, 16A 60 Hz
Power consumption • 230/400V power supply: 6,300 Watts

± 10%
• 200-210V power supply: 5,300 Watts
± 10%
Water supply Minimum flow rate 5L/min

Minimum temperature 5°C
Maximum temperature 20°C
Hardness 0.7 to 2 mmol/L (CaCO3) = 7 to 20°Fh (TH)
Minimum pressure 1 bar
• If the pressure is above the maximum recommended threshold, a

pressure regulator valve must be installed.
• If the pressure is below the minimum recommended threshold, a
pressure booster pump must be installed.
Demineralized Minimum pressure 1 bar

water supply
Minimum flow 0.7L/min
Maximum flow 2.3L/min
Vessel working pressure 0 to 1.6 bar
Vessel design pressure 1.7 bar
Production capacity 20L max. (medium + demineralized water +
additional elements)
Installation category 2 (according to European standard ECI664)
Overvoltage category Category II
Pollution degree Category 2
Sound level < 70 db
Dimensions Instrument alone 590 x 700 x 650 mm (cover closed)
(W x H x D) Instrument (packed) 1,200 x 930 x 800 mm
Weight Instrument alone 90 kg

Instrument (packed) 110 kg
Temperature accuracy ± 0.5°C in stabilized phase

System Basics System Description and Basic Operations
TFT resistive touchscreen Size: 15.24 x 9.14 cm (7 inches)

Resolution: 800 x 480 px
Keypad 8 physical keys

Fuse 16A 10 x 38 mm T 500 VAC for serial
number inferior or equal to 10090046 or
serial numbers starting with 5 et 6)
All-pole circuit breaker All-pole circuit breaker from serial number
superior to 10090046 and not starting with 5
or 6
Lithium battery • Panasonic CR2032 - Nominal charge: 3V
225 mAh - Continuous standard charge:
0.2 mA
• Renata CR2032 - Nominal charge: 3V
235 mAh - Continuous standard charge:
0.2 mA
System Basics
Overview of Operation Elements
1 Media temperature probe

2 0.2 µm filter
3 Air compressor
4 Stirrer
5 Heating elements
6 Stirrer motor
Demineralized water
Media
Air

Overview of Operation Elements System Description and Basic Operations
MASTERCLAVE® 10 and MASTERCLAVE® 20 use the double-boiler principle. For

MASTERCLAVE® 10, the net capacity medium + demineralized water + additional elements is
1-to-10L. For MASTERCLAVE® 20 the net capacity medium + demineralized water +
additional elements is 1-to-20L.
Sterilization temperatures can vary between 25°C and 125°C, and dispensing temperature
can vary between 25°C and 78°C. Temperatures are controlled with heating and cooling
commands to deal with the various preparation cycles. The preparation is homogenized via a
removable magnetic stirrer, placed at the bottom of the vessel.

4 System Installation and Configuration
Unpacking the Instrument
The box is heavy. Exercise caution when moving lifting, and unpacking the instrument to
avoid injury. It is recommended to have at least three people to lift the instrument. Remember
to bend your knees to avoid back injury.
Note: Always keep the packaging (box, pallet, foam, and straps) for future transport.
1. Remove the screws from the base of the box.

2. Remove the box and packing foam.
3. Remove the straps that secure the instrument.
4. Check that the device has not been damaged during transport.
5. Confirm that the accessories listed below are present.
1 priming tube 1 non-return valve

1 set of seals for priming nozzle 1 priming nozzle
1 brush for vessel cleaning 4 meter hose
1 water inlet filter with clamps • 2 hose clamps (MASTERCLAVE® 10)
®
• 3 hose clamps (MASTERCLAVE 20)
2 draining caps

Installing the Instrument System Installation and Configuration
Installing the Instrument
DANGER
This procedure must be performed by a trained technician.
IMPORTANT: Never use the lid or cover handles to transport, move or lift the instrument.
1. For optimal operation, follow the steps below to install the instrument:
• Set the instrument on a clean, stable, and horizontal surface.
• Set the instrument near a filtered water supply (for example, a water discharge
outlet, water inlet, or main supply) and near a single-or-three-phase power socket.
CAUTION: A filter must be installed upstream of the instrument to

avoid obstruction of the water circuit with particles.
• For a MASTERCLAVE® 10, set at a height which allows the user to work in an
ergonomically comfortable position.
2. Make sure there is easy access to the rear and side connection ports and to the power
socket.
3. Make sure the installation complies with the altitude, temperature, and humidity limits
as specified in the Technical Data and Specifications section.
4. The instrument connection to the filtered water supply must comply with the following
criteria:
Water inlet Water outlet

Minimum flow rate: 5L/min 90°C resistant pipe
Minimum pressure: 1 bar Siphon installed
Maximum pressure: 4 bar Minimum pipe section: 40mm
Minimum temperature: 5°C No outlet pressure
Maximum temperature: 20°C
Water hardness: 0.7 to 2 mmol/L
(CaCO3) = 7 to 20°Fh (TH)

Connecting the MASTERCLAVE® 10 to the Power Supply Network System Installation and Configuration
5. If connecting a MASTERCLAVE® 20, connect the instrument to a demineralized water

supply.
• In the case of hard water, install a descaling cartridge upstream of the filter.
• If the instrument is liable to be moved frequently, install quick connectors on the water inlet
and outlet pipes.
Connecting the MASTERCLAVE® 10 to the Power Supply Network
DANGER
Connecting the MASTERCLAVE® 10 to the Single-Phase Power Network

Table 1: Technical specifications required for a single-phase power supply
Voltage 230V ~ ± 10 % single-phase

Current 16A
Frequency 50Hz
Power consumption 3,300W ± 10%

Connecting the MASTERCLAVE® 10 to the Three-Phase Power
Network without a Neutral Power System Installation and Configuration
1. If the cable is not equipped with the connector supplied by bioMérieux®, check that it
complies with local regulations and with the following specifications:
N Neutral
E Ground
1 Phase 1
2 Phase 1
3 Phase 1
2. Connect the power-supply cable to the instrument and tighten the connector locking
device.
3. Connect the instrument to the power supply.
IMPORTANT: Never touch exposed parts of the female connector when connecting the power
supply cable to the mains power supply.
Connecting the MASTERCLAVE® 10 to the Three-Phase Power Network without a

Neutral Power
Table 2: Technical specifications required for a three-phase power supply:
Voltage 200-210V ~ ± 10 % three-phase with no

neutral
Current 16A per phase
Frequency 60Hz
CAUTION: It is necessary to change the electrical configuration of the

instrument to adapt it to the mains power supply.
1. Disconnect the instrument.

2. Slide down to remove the right-hand panel.

Network without a Neutral Power System Installation and Configuration
3. Turn the switch clockwise.
4. Put the side panel back in place.

5. Check that the connector used to connect the instrument to the power supply complies
with the following specifications:

Connecting the MASTERCLAVE® 20 to the Power Supply Network System Installation and Configuration
N Not connected
E Ground
1 Phase 1
2 Phase 2
3 Phase 3
6. Connect the power-supply cable to the instrument.
7. Tighten the power-supply cable locking device.
8. Connect the instrument to the three-phase power socket.
IMPORTANT: Never touch the female connector when connecting the power supply cable to
the mains power supply.
Connecting the MASTERCLAVE® 20 to the Power Supply Network
DANGER
Connecting the MASTERCLAVE® 20 to the Three-Phase + Neutral Power Network

Table 3: Technical specifications required for Three-Phase + Neutral Power Network:
Voltage 230/400V ~ ± 10% three-phase + neutral

Current 16A
Frequency 50Hz
1. If the cable is not equipped with the connector supplied by bioMérieux®, check that it
complies with local regulations and with the following specifications:

Network with no Neutral Power System Installation and Configuration
N Neutral
E Ground
1 Phase 1
2 Phase 2
3 Phase 3
3. Tighten the connector locking device.
4. Connect the instrument to the power-supply.
IMPORTANT: Never touch exposed parts of the female connector when connecting the
power-supply cable to the mains power supply.
Connecting the MASTERCLAVE® 20 to the Three-Phase Power Network with no

Neutral Power
Table 4: Technical specifications required for Three-Phase Power Network with no
Neutral:
Voltage 200-210V ~ ± 10% three-phase with no

neutral
Current 16A per phase
Frequency 60Hz
CAUTION: It is necessary to change the electrical configuration of the

instrument to adapt it to the mains power supply.
1. Disconnect the instrument.

2. Slide down to remove the right-hand panel.

Network with no Neutral Power System Installation and Configuration
3. Turn the switch clockwise.
4. Put the side panel back in place.

5. Check that the connector used to connect the instrument to the power supply complies
with the following specifications:
N Not connected
E Ground
1 Phase 1
2 Phase 2
3 Phase 3
7. Tighten the power-supply cable locking device.
8. Connect the instrument to the three-phase power socket.
IMPORTANT: Never touch the female connector when connecting the power-supply cable to
the mains power supply.

Connecting the MASTERCLAVE® 20 to a Demineralized Water
Source System Installation and Configuration
Connecting the MASTERCLAVE® 20 to a Demineralized Water Source
The MASTERCLAVE® 20 can be connected to a demineralized water supply source to fill the
water bath.
The MASTERCLAVE® 20 can be connected to:
• a demineralized water line already available in the building.
• a demineralized water tank, using an e-pump.
Connecting the MASTERCLAVE® 20 to a Demineralized Water Tank

The MASTERCLAVE® 20 can be connected to a demineralized water container by using a e-
pump.
1. Connect the MASTERCLAVE® to a demineralized water source as specified below:
2. Press .
3. Press .
4. Press .
5. Press .
6. Press to select demineralized water supply via a pump.
Installing the Auto Water Filling for Tank for the MASTERCLAVE® 20
The Auto Water Filling kit is used to connect the MASTERCLAVE® 20 to a tank of
demineralized water.
1. Turn OFF the instrument before connecting the e-pump.

2. If necessary, install the water filter on the auto filling tube.

Connecting the MASTERCLAVE® 20 to a Demineralized Water
Supply Network System Installation and Configuration
Note: The use of the Sartorius 150 5231307H4-OO-B water filter is recommended. Contact
Sartorius or approved Sartorius distributors for more information about the filter or for
an equivalent reference.
a) Cut the auto filling tube:
b) Position the water filter.

c) If necessary, add hose clamp.
3. Connect the tube to the supplement addition port and to the demineralized water
connection on the left side of the instrument.
4. Connect the demineralized water tube to the water inlet on the rear side.
5. Drive out the air bubbles.
Connecting the MASTERCLAVE® 20 to a Demineralized Water Supply Network

The MASTERCLAVE® 20 can be connected to a demineralized water supply source for easier
water bath or culture-medium vessel filling.
1. Connect the instrument to the demineralized water supply adhering to the following
criteria:
demineralized water inlet

Minimum flow rate: 0.7L/min
Minimum pressure: 1 bar
Maximum flow rate: 2.3L/min
Maximum pressure: 2 bar
2. Press .
3. Press .
4. Press .
5. Press .
6. Press to select a demineralized water supply via a pressurized demineralized
water line.
Note: If the pressure exceeds 2 bar, a pressure reducer must be installed.

Installing the Auto Water Filling for Network for the MASTERCLAVE®
20 System Installation and Configuration
Installing the Auto Water Filling for Network for the MASTERCLAVE® 20
The Auto Water Filling kit is used to connect the MASTERCLAVE® 20 to a network of
demineralized water.
1. If necessary, install the water filter on the auto filling tube.
Note: The use of the Sartorius 150 5231307H4-OO-B water filter is recommended. Contact
Sartorius or approved Sartorius distributors for more information about the filter or for
an equivalent reference.

Starting the MASTERCLAVE® System Installation and Configuration
a) Cut the auto filling tube:
b) Position the water filter.

c) If necessary, add a hose clamp.
2. Connect the tube to the supplement addition port and to the demineralized water
connection on the left side of the instrument.
3. Check that the MASTERCLAVE®20 is connected to the demineralized water source.
4. Drive out the air bubbles.
Starting the MASTERCLAVE®
Prior starting the MASTERCLAVE®, ensure:

• You have connected the instrument to a power socket.
• The instrument is connected to the recommended water supply.
1. Lift the cover and the lid to remove the stirrer transport shims from the vessel.
2. Place the switch at the front of the instrument on "ON".
The MASTERCLAVE® is switched on.
3. Choose a country with localization settings nearest to your own country. Customize the
localization settings to adapt them to your own country.
4. Press to start the cycle.
5. Follow the instructions on the screen to perform a cycle.
User Management
It is possible to create, modify, or remove user accounts. Only Administrators are allowed to
manage user account security settings.
Activating/Deactivating the Users Option

It is possible to activate, or deactivate the User management option, which is used to create
user accounts for increased traceability.
1. Press .
The configuration screen is displayed.
2. Activate or deactivate the Users option.

User Access Rights System Installation and Configuration
When the Users option is activated, the user is automatically disconnected and needs
to open a new session to use the device.
User Access Rights

Table 5: Users
Actions Technician Manager Administrator

User account X
management
User account security X
(password policy)
My account X X X
modification
User creation X X
User modification X X
User deletion X X
Table 6: Account Parameter

Configure my user X X X
account
Update my password X X X
Update a user X X
password
Associate my account X X X
to my access card
Associate an account X X
to an access card
Table 7: Programs

Program management X
Programs creation, X X
modification, deletion
Table 8: Cycle History

Display and export X X X
cycle history
Modify cycle history X
settings

User Access Rights System Installation and Configuration
Table 9: System Configuration

Localization X
Power X
Sounds X
Date/time X
Peripherals X
Import/Export X
Table 10: Equipment Settings

Metrology Display X X X
certificates
Calibration X X X
Adjustment X X
Metrology X X
settings
configuration
LIMS X
Ingredient Barcode X X
Operation Operation X X
Traceability X X
I volume X
activation/
deactivation
Emails Message X
management
Recipient X X
SMTP server X
Test X X
Table 11: Support

Support menu connection X X X
Information X X X
Update (firmware) X

Creating a User Account System Installation and Configuration

Servicing Water bath X X X
draining
Water bath filling X X X
(M20)
Preventive X X
maintenance
Sensor X
management
Descaling X
Counters X
Remote control X X X
Tests X
Table 12: Program Selection

Program selection X X X
Table 13: Log In/Log Out

Log in/log out X X X
Table 14: Start a Cycle

Start a cycle X X X
Table 15: Enter Media Traceability Information

Enter media X X X
traceability information
Table 16: Help Function

Help function X X X
Creating a User Account

1. Press , then .
2. Press to access the Users screen.
3. Press .
4. Complete the fields.

Modifying a User Account System Installation and Configuration
Note: Assign a barcode to the user account in the Access Badge field.
5. Press to confirm.
Modifying a User Account

1. Press , then .
3. Select the account to modify.
4. Press .
5. Modify the fields.
Note: Assign a barcode to the user account in the Access Badge field.
Deleting a User Account

1. Press , then .
3. Select the account to delete.
4. Press .
Configuring the User Account Settings

Configure the security settings for the user accounts.
• Decide how to manage passwords.
• Decide whether to authorize anonymous sessions.
• Manage automatic disconnections.
1. Press .
3. Press .
4. Define the user account security settings and password management procedures.
Note: If passwords are used, assign a password to each new user account creates. Users
can change the password in their user account. See Modifying My User Account.
Changing a User Session

1. Press .
The current user session is selected by default.
2. Select another user session.
4. Enter a password, if required.
Managing My User Account

It is possible to modify your user account and end your session.

Ending My User Session System Installation and Configuration
Ending My User Session

End your session so that another user can access the device.
1. Press .
2. Press .
Your session has ended.
Modifying My User Account

Change your name and identifier, assign an access badge, or change your password. Only
Administrators and Managers may modify their access rights.
1. Press , then .
2. Select your account.
3. Press .
4. Modify your data.
Configuring the Software Settings
Only Administrators are allowed to configure system settings.
Exporting the Configuration Settings for the Instrument

Export the configuration settings for the instrument via the peripherals.
• It is recommended to export the configuration before a firmware update and after a setting
of the instrument.
• It can be used to duplicate the configuration on another instrument.
1. Press , then .
2. Press .
3. Press to access the IMPORT/EXPORT screen.
4. Press .
5. Select the chosen peripheral in the EXPORT TO screen.
6. Select the folder where you want to store the configuration settings file in the right-
hand column.
7. Select the folder where you want to store the configuration settings file.
Importing the Configuration Settings

Import the configuration settings for the equipment from the peripherals.
1. Press , then .
2. Press .
3. Press to access the IMPORT/EXPORT screen.
4. Press .
5. Select the chosen peripheral in the IMPORT FILE screen.

Configuring the Localization Settings System Installation and Configuration
6. Choose the configuration to import by selecting a folder, then a file in the right-hand
column.
The device will boot up again.
Configuring the Localization Settings

Configure the keyboard, date and time format, language, units, and numbers.
1. Press , then .
2. Press .
3. Press to access the LOCALIZATION screen.
Adjusting the Energy Settings for the Equipment

Adjust the energy settings for greater readability and configure the sleep feature to save
energy.
1. Press , then .
2. Press .
3.
Press to access the POWER screen.
4. Configure the settings.

Configuring the Current Date, Time, and Time Zone

Configure the current Date, Time, and Time Zone and enable or disable the automatic date/
time and Time Zone Synchronization feature.
1. Press , then .
2. Press .
3. Press to access the DATE & TIME screen.
5. Enter the date and select the time.
Enabling/Disabling Sounds
Enable or disable sounds emitted during certain events.
1. Press , then .
2. Press and enter the Administrator password.
3. Press then .
4. Enable or disable the sounds.

Updating the Firmware System Installation and Configuration
Updating the Firmware
Activate or deactivate the automatic update installation and search feature and update the
firmware.
• If the user management feature is activated, log on as Administrator.
• A backup of the instrument configuration is exported (see Exporting the Configuration
Settings for the Instrument).
• The firmware is available on a USB flash drive or via the embedded FTP and is connected
to the instrument.
1. Press , then .
2. Press then to access the Update screen.
3. Press on the Update screen.
4. In the Content panel, select the host.
The host name appears in the File Manager panel.
5. Select the firmware file (.amf) and confirm.
®
• The update begins and the MASTERCLAVE restarts.
• A message Firmware Update completed is displayed.
6. Press OK.
7. When the update is finished, log on as usual.
8. Press , then .
9. Press , then .
10. Check the firmware version name to confirm the firmware has been uptated.

5 Workflow and Instructional Procedures
Starting the System
Before starting the system and creating workflows and instructions, confirm the following:
• The instrument is connected to a power socket.
• The instrument is connected to the water supply as recommended.
1. Set the MASTERCLAVE® on/off switch to ON.
The MASTERCLAVE® starts.
2. If the Users option is activated, select a user account.
4. If the Password Management is activated, enter the password.
Shutting Down the System
1. Check that there is no operation in progress.

2. Set the MASTERCLAVE® on/off switch to OFF.
IMPORTANT: Do not get the power socket wet or leave it in water when disconnecting the
instrument.
Managing Programs
Activating/Deactivating the Programs Management

Program Management allows for creation of programs and use of predefined programs to
optimize certain operations. Only administrators can activate or deactivate the program
management.
1. Press .
The configuration screen is displayed.
2. Activate or deactivate the Programs option.
Creating a Simple Program

1. Press , then .
2. Press to access the Programs screen.
3. Press .
4. Under the Identity tab, enter the program name and complete the fields:
• FAST mode: quickest mode to produce media.

Creating a Program for Supplement Addition at a High Temperature Workflow and Instructional Procedures
• NORMAL mode: best mode to save resources and cycle duration. It is the
recommended mode for standard use.
• ECO mode: best mode to save resources but the cycle is longer.
• CUSTOM mode: only mode dedicated to specific application for complex media
(R&D application).
5. Under the Cycle tab, press each part of the graph to configure the settings.
F0: equivalent exposure time at 121.11°C than the actual exposure time at a variable
temperature calculated for an ideal microorganism with a temperature coefficient of
destruction of 10°C (Z).
6. Under the Options tab, choose checking mode F0, coefficient Z and the preparation
type.
• Preparation field:
◦ Select the medium for agar media preparation
◦ Select the diluent for broth preparation
7. Under the Formula tab, press to add the supplements.
8. Press each cell in the table to configure each supplement.
Creating a Program for Supplement Addition at a High Temperature

1. Press , then .
3. Press .
4. Under the Identity tab, enter the program name and complete the fields.
5. Under the Cycle tab, press to add a stage.
6. Press the second stage to configure the following for the new stage:
• temperature (<78°C)
• duration
• activation of the supplement addition
• compensation required during supplement addition stage (recommended if added
ingredient represents over 4% of total volume).
• the alarm to be triggered in case the temperature rises above the limit.
Creating a Program to Add Supplements at a Dispensing Temperature

1. Press , then .
3. Press .
4. Under the Identity tab, enter the program name and complete the fields.
5. Under the Cycle tab, press to add a stage.
6. Press the second stage and set the dispensing temperature.
7. Set stage duration to 2 minutes.
8. If required, adjust the following settings:
• stirring speed
• activation of the supplement addition
• compensation required during supplement addition stage (recommended if added
ingredient represents over 4% of total volume).
• the alarm to be triggered in case the temperature rises above the limit.

Deleting a Program Workflow and Instructional Procedures
Deleting a Program
1. Press , then .
3. Select the program to delete.
4. Press .
Filling the Water Bath
Filling the Water Bath Manually

• Only use demineralized water.
1. Remove the culture-medium vessel.
2. Check that the demineralized water level is between the minimum and maximum
markers.
®
• MASTERCLAVE 10: minimum volume 1.8 liters; maximum volume: 2.2 liters
• MASTERCLAVE® 20: minimum volume 2.5 liters; maximum volume: 4 liters
Using the Automatic Water Bath Filling System (MASTERCLAVE® 20)

• The water bath has already been drained.
• The instrument is connected to a demineralized water source.
1. Press , then .
2. Press .
3. Press .
4. Follow the instructions on the screen.
5. Select the type of filling process used:
• With the filling kit
• With the shower
While the water bath is filling, a disc shows the fill level.
Performing Cycles
Performing a Cycle (Without Program Management)

To use the automatic filling option:
• the instrument must be connected to a demineralized water source.
• the filling system must be connected to the demineralized water inlet on the left-hand side
of the instrument.
Note: Automatic filling should only be used for broth preparation. It is not recommended to use it
for agar preparation.
1. Press .
2. Adjust the cycle settings:

Performing a Cycle (With Program Management) Workflow and Instructional Procedures
• To add one or more stages, press .

• To configure the stages, click each stage and complete the data fields.
• To configure the heating, cooling, and dispensing phases, press on the
corresponding sections of the graph.
3. Press .
4. Finalize the cycle setting configuration:
• select medium for agar media preparation.
• select diluent for broth preparation.
• select for manual media vessel filling.
• select for automatic filling using the shower option.
• select for automatic filling using filling kit option.
Note: See Performing a Delayed Start Cycle for additional information to perform an
autostart cycle with the MASTERCLAVE® 20.
CAUTION: Priming the system is mandatory before each automatic

filling. Press to prime in an external container.
6. Follow the on-screen instructions and press to confirm each preparation stage.
The graph is displayed on-screen as the cycle progresses.
DANGER
• Never unplug the instrument while a cycle is in progress. To power down
the instrument, wait until the current cycle has ended, switch off the
instrument, then disconnect from the power supply.
• Avoid touching the metal part of the top panel when the lid is open.
• Never open the vessel lid when a cycle is in progress.
Performing a Cycle (With Program Management)

To use the automatic filling option:
• the instrument must be connected to a demineralized water source,
• the filling system must be connected to the demineralized water inlet on the left-hand side
of the instrument.
Note: Automatic filling should only be used for broth preparation. It is not recommended for agar
preparation.
1. Press .
2. Press to access the Active programs screen.
3. Select a program in the list.
5. If required, change the number of stages and their settings.

Performing a Delayed Start Cycle Workflow and Instructional Procedures
6. Press .
7. Adjust the cycle settings:
• Select medium for agar media preparation.
• Select diluent for broth preparation.
• Select for manual media vessel filling.
• Select for automatic filling using the shower option.
• Select for automatic filling using filling kit option.
Note: See Performing a Delayed Start Cycle for additional information to perform an
autostart cycle with the MASTERCLAVE® 20.

9. Follow the on-screen instructions and press to confirm each preparation stage.
The graph is displayed on the screen as the cycle progresses.
DANGER
• Never unplug the instrument while a cycle is in progress. To power down
the instrument, wait until the current cycle has ended, switch off the
instrument, then disconnect from the power supply.
• Avoid touching the metal part of the top panel when the lid is open.
• Never open the vessel lid when a cycle is in progress.
Performing a Delayed Start Cycle

To program a delayed start cycle, add a setting during the preparation phase.
To start a cycle:
• The Cycle data screen is displayed.
• For the automatic filling option, the instrument is connected to demineralized water source.
• The filling system is connected to the demineralized water inlet on the left-hand side of the
instrument.
Note: Automatic filling should only be used for broth preparation. It is not recommended for agar
preparation.
1. Switch ON the Delayed Start option.

2. Press and to select the required date and time.
3. Select .

Pressurizing the Instrument Workflow and Instructional Procedures

Note: It is recommended to stay by the MASTERCLAVE® during initialization until the Delayed
Start screen is displayed.
After a progress bar is displayed, the screen displays the delayed start details. A blue
indicator light will start blinking on the MASTERCLAVE®, and it will start the cycle as
programmed.
Pressurizing the Instrument
At this point, the cycle is Dispensing phase and the light indicator is blinking green.
At the end of the preparation cycle, pressurize the instrument to optimize the prepared
medium dispensing phase.
WARNING
In case of e-pump connection, turn off the instrument before connecting the
e-pump.
1.
< 78°C.
2. Install the tube set.

3. Check that the pump is locked correctly.
4. Press .
5. Enter the required pressure (0.3 bar recommended).
6. Press the Under pressure button.
A pop up window appears. It has to disappear before moving to the next step.
7. Wait for the pop up window to disappear before moving to the next step.
8. Start the dispensing phase.
9. Release the pressure at the end of the dispensing phase:
®
• If the MASTERCLAVE is connected to a water outlet, press the Release the
pressure button. Then press .
®
• If the MASTERCLAVE is not connected to a water outlet:
1. Check that the dispensing nozzle is inserted in a container.
2. Press the Release the pressure button. A pop up appears.
3. Open carefully the pump head to release manually the pressure in the
container.
4. Once the pressure is down to 0, press the Release the pressure button.
5. Press .

Dispensing with an e-pump Workflow and Instructional Procedures
Dispensing with an e-pump
At this point:
• The cycle is in dispensing phase and the light indicator is blinking green.
• Only a single e-pump can be connected to the instrument.
1. Switch OFF the instrument.
2. Connect the e-pump at the rear of the instrument.
3. Switch ON the instrument.
4. Install the priming tube set.
5. Press .
6. Press NO PUMP and select Active.
The pump is detected and the e-pump light indicator turns on.
7. Press >.
8. Enter the dispensing settings.
9. Press > twice to configure the advanced dispensing option settings and press > twice
to return to the dispensing settings.
10. Press Calibrate and complete the fields.
11. Press to prime the pump.
12. Press Start to dispense a dose in a graduated container.
13. Enter the volume of the dispensed dose.
14. Check the volume:
• If it is equal to the volume to be dispensed, press Validate.
• If it is unsatisfactory, repeat the calibration as often as necessary and press
Validate.
15. Press Start to initiate the dispensing process.
Displaying and Exporting Traceability Information
All the cycle data are collected and saved in the Cycle History menu.
Note: The cycle history data can be saved for 90 days or infinite: to configure, Press , then .
Then press .
1. Press , then .
2. Select .
3. Select the year and press .
4. Select the month and press .
5. Select the week and press .
6. Select the cycle and press .
• On each selection screen, allows to export CSV files of the year/month/week.
• appears only when a printer has been installed (physical or virtual). It allows to
print 2D barcodes with prepared media traceability. It can be scanned on a Dilumat
or a PMi using the 2D barcode reader.

Managing the Peripherals Workflow and Instructional Procedures
• appears only when a printer has been installed (physical or virtual). Allows to
print out cycle data with graph. Graph is only available with a built-in printer.
• allows to export cycle data and traceability in CSV format on peripheral devices.
• allows to export a PDF document based on the ISO 11133 recommendation
with Temperature graph, Cycle data, Traceability, Quality Control template step.
Managing the Peripherals
Access more features such as data management, updates, etc., by adding physical or virtual
peripherals sur as USB or WIFI stick, Ethernet, printer, virtual printer, number pad, barcode
reader, embedded FTP, remote FTP. Only Administrators may configure peripherals
connected to the instrument.
Activating/Deactivating the Embedded FTP Server

It is possible to activate/deactivate the embedded FTP server to store the data.
1. Press , then .
2.
Press , then .
3. Select the embedded FTP server in the list of peripherals.

4. Press the on/off button to activate/deactivate the embedded FTP server.
Note: It is possible to connect to the embedded FTP server from the computer by opening
an anonymous session using the Administrator password.
Enabling/Disabling the i™ Volume

The i™ Volume feature allows the various equipment in the bioMérieux Blue Line range to
interact with each other. When a MASTERCLAVE® and a DILUMAT® Expert or
a DILUMAT® Expert EVO are installed in the same network, the volume remaining in the
culture medium vessel of the MASTERCLAVE® on the DILUMAT® is displayed in real time.
The I volume feature prefills the diluent traceability information (name, expiry date, batch
number, volume) on the DILUMAT®, if completed before it is on the MASTERCLAVE®. In case
of multiple connection, each DILUMAT® displays the remaining volume in
the MASTERCLAVE® to which they are connected.
Enable the i™ Volume feature by selecting the available diluents marked in blue.
1. Press , then .
2. Press then .
™
3. Select the i Connect tab.
4. Enable/disable i™ Volume.
Connecting a USB Stick

It is possible to use a USB stick to store data or firmware updates.
Note: It is possible to connect the USB stick from any screen.

Configuring a Wifi Stick Workflow and Instructional Procedures
1. Press , then .
2.
Press , then .
3. Connect the USB stick at the rear of the instrument.

The USB stick is automatically detected and displayed in the list of peripherals.
IMPORTANT: Deactivate the USB stick before removing it to avoid loss of data.
Configuring a Wifi Stick

It is possible to use a WIFI stick to access a network and manage the data. It allows file
transfer (result, PDF, FTP connection), I™ volume, and e-mail management with a wireless
connection.
Note: It is recommended to contact the IT department to configure the WIFI stick.
Deactivate the Ethernet before activating the WIFI stick.

1. Press , then .
2.
Press , then .
3. Insert the Wifi stick at the rear of the instrument.

The Wifi stick is automatically detected and displayed in the list of peripherals.
4. Press the on/off button to enable the Wifi stick.
5. Press to configure the Wifi stick.
Configuring the Ethernet

It is possible to use an Ethernet connection to access a network and manage data.
Deactivate the Wifi stick before activating the Ethernet.
Use a category 5 shielded cable to connect to the Ethernet.
1. Press , then .
2.
Press , then .
3. Connect the Ethernet cable at the rear of the instrument.

The Ethernet cable is automatically detected and displayed in the list of peripherals.
4. Press the on/off button to activate the Ethernet.
The device will connect to the network.
5. Press to display the IP settings.
By default, the device is configured in DHCP mode.
Configuring a Remote FTP Server

It is possible to configure a remote FTP server to store the data.
1. Press , then .
2.
Press , then .

Connecting a Physical Printer Workflow and Instructional Procedures
3. Press .
4. Select remote FTP server, then press .
The remote FTP server is included in the list of peripherals.
Connecting a Physical Printer

It is possible to use a physical printer to print the labels.
1. Press , then .
2.
Press , then .
3. Connect the printer at the rear of the instrument.

The printer is automatically detected and displayed in the list of peripherals.
Note: The printer is configured by default.
4. Press to change the default configuration.
Creating a Virtual Printer

It is possible to use a virtual printer to store data on a USB stick, an embedded FTP server, or
a remote FTP server.
The virtual printer host is connected to the instrument.
1. Press , then .
2.
Press , then .
3. Press .
4. Select Virtual Printer.
The virtual printer is displayed in the list of peripherals.
Note: The virtual printer is configured by default.
8. Press to change the default configuration.
Changing a Label Model

It is possible to change the instrument default label models.
The printer is connected or a virtual printer have been created.
1. Press , then .
2.
Press , then .
3. Select a printer, then press .

4. Select the type of label to change.

Importing a Label Model Workflow and Instructional Procedures
5. Select a different label model.

Importing a Label Model

It is possible to customize label models by importing personal templates.
• The printer is connected or a virtual printer have been created.
• The label template have been stored on an external storage device (USB stick, embedded
FTP server, or remote FTP server).
1. Press , then .
2.
Press , then .
3. Select a printer, then press .

4. Select the type of label to customize.
5. Press .
6. Select and import the label template from the external storage device.
Configuring a Barcode Reader

It is possible to use a barcode reader to scan the labels.
1. Press , then .
2.
Press , then .
3. Connect the barcode reader at the rear of the instrument.

The barcode reader is automatically detected and in the list of peripherals.
4. Press to configure the barcode reader.
IMPORTANT: A barcode reader is configured according to the language of the country where
it is installed, like a keyboard. In France, for instance, keyboards are configured
in AZERTY format, while in English-speaking countries (UK, US, etc.) keyboards
are configured in QWERTY format. Please select the country code for the
localization settings needed for the barcode reader. Otherwise, the reader will
not be able to read the barcodes. Test the reader on a barcode with the content
displayed in the test zone to check if the reader configuration is correct.
5. Select the language for the localization settings you need for the barcode reader.
Configuring the Communication Settings for the LIMS
Configure the communication settings to allow connection with the LIMS.

To configure the LIMS, contact bioMérieux customer service.
1. Press , then .
2. Press .
3. Press .

Configuring E-Mail Alerts Workflow and Instructional Procedures

Configuring E-Mail Alerts
The instrument is connected to the network.
Note: It is recommended to contact the IT department to configure the e-mail parameters.
1. Press , then .
2. Press .
3. Select .
4. Configure the e-mails options.
Managing Messages
Reading a Message
A flashing envelope indicates that there is a new message, regardless of which screen is
open.
1. Press .
The Message screen is displayed.
2. Select the message to read, then press .
• The message is acknowledged.

• It will remain in the list of messages.
• The envelope no longer flashes.
Deleting a Message
After reading the messages, delete them from the message list.
1. Press , regardless of which screen is open.
The Messages screen is displayed.
2. Select the message to delete, then press .

6 Consulting the Instrument Information
To change the name of the instrument and see the instrument technical data and program
versions use the following steps:
1. Press , then .
2. Press .
3. Press .
4. If needed, change the instrument name and see the available information.

7 User Maintenance
For service information, contact bioMérieux or your local distributor (contact information
Calibration and Adjustments
Calibrating the Culture-Medium Vessel Sensor

An independent temperature sensor, which provides and instant reading, has been installed.
Note: It is recommended to calibrate the culture-medium vessel sensor once a year.
1. Press , then .
2.
Press then to access the Metrology screen.
3. Select media vessel sensor.

4. Press .
5. Enter the required temperature level and confirm it.
Adjusting the Culture-Medium Vessel Sensor

• The current adjustment certificate has been printed.
• An independent temperature sensor providing an instant reading has been installed.
1. Press , then .
2.
3. Select media vessel sensor.

4. Press .
Note: The system displays two adjustment levels.

• Adjustment needs to be performed at least on two temperature values.
• It is possible to add more values.
• Click in each column to enter the required values.
• It is possible to delete a value by clicking .
5. Enter the required temperature level.

6. Press .
Calibrating the Demineralized Water Flow Meter

• It is necessary to have at least a 2L container.
• The MASTERCLAVE® 20 is connected to a demineralized water supply.
Note: It is recommended to calibrate the demineralized water flow meter at least once a month.

Adjusting the Demineralized Water Flow Meter User Maintenance
1. Press , then .
2.
3. Select Demineralized Water Flow Meter.

4. Press .
5. Follow the instructions on the screen and confirm each stage.
Adjusting the Demineralized Water Flow Meter

It is necessary to have at least a 2L container.
1. Press , then .
2.
3. Select demineralized water flow meter.

4. Press .
Printing a Certificate
It is possible to print calibration and adjustment certificates with a physical or virtual printer.
The labels are printed in .png format and may be stored on a USB stick, FTP server, etc.
Check that all the peripherals required for printing have been configure. See Managing the
Peripherals.
1. Press , then .
2. Press .
3.
Press .
4. Select the sensor required.

5.
Press .
6. Select a certificate type.

7. Press .
The certificate will be printed as a label or saved in .png format on a USB stick or an
FTP server.
Restore the Calibration and Adjustment Certificates

It is possible to restore the default settings for the calibration and adjustment certificates.
1. Press , then .
2. Press .
3.
Press .

5.
Press .
6. Select a certificate type.

7. Press to restore the default settings.

Defining the Maximum Permissible Error (MPE) User Maintenance
Defining the Maximum Permissible Error (MPE)

It is possible to define the Maximum Permissible Error (MPE). Light Indicators illuminate if the
MPE is exceeded when implementing a calibration.
1. Press , then .
2. Press , then .
4. Press .
Defining the Validity Period for Metrology Certificate

It is necessary to define the validity of metrology certificates or deactivate the option.
1. Press , then .
2. Press , then .
4. Press .
Cleaning Procedures
Cleaning the Instrument
CAUTION:
• Never clean the instrument when in operation.
• Never spray cleaning product directly onto the instrument.
• Do not use aggressive chloride or acid-based products.
• Do not use abrasive sponges.
• Clean with rubbing alcohol 70° and/or hot water
1. Set the MASTERCLAVE® on/off switch to OFF.

2. Disconnect the instrument from the mains power socket.
3. Clean the following with a soft cloth, cleaning fluid or warm water:
• culture medium vessel
• stirrer
• inside of the lid
4. Prime the priming tube connector and the priming tube.
5. Rinse the priming tube connector and the priming tube in warm water.
Performing a Cleaning Cycle

IMPORTANT: Perform a cleaning cycle monthly.

Cleaning the Non-Return Valve User Maintenance
1. Fill the water bath with demineralized water to the maximum level.
2. Fill the culture medium vessel with 5L of demineralized water.
3. Start a cycle with the following settings:
• Sterilization at 121°C for 30 minutes
• Dispensing at 60°C
4. Drain the water bath.
Cleaning the Non-Return Valve

The non-return valve should be cleaned weekly.
1. Unscrew the air filter from the non-return valve.
2. Unscrew the non-return valve from the lid.
3. Use the brush provided with the MASTERCLAVE® to clean the valve with hot water or
use ultrasonic bath.
4. Wrap the seal and valve in aluminum and autoclave.
5. Be sure to replace the seal before fitting the valve back on the lid.
Cleaning the Automatic Filling Kit

The automatic filling kit should be cleaned every 3 months.
1. Unscrew the kit from the lid.
2. Disconnect the kit from the demineralized water inlet on the left side of the instrument.
3. Use a swab to clean the part screwed onto the lid.
4. Rinse with warm water.
Cleaning the Auto Water Filling for Tank (MASTERCLAVE® 20)

The e-pump is connected to the MASTERCLAVE®.
Decontaminate the auto water filling for tank tubes:
• when installing the system,
• when the demineralized water source has not been used for a long time,
• when after 1 month of using the demineralized water source,
• when changing the water source.
1. Install the demineralized water tubing in the e-pump (use the specific tubing part
between the two plastic connectors “pump part”).
2. Place the priming nozzle of the outlet tubing in the demineralized water tank.

Cleaning the Auto Water Filling for Network (MASTERCLAVE® 20) User Maintenance
3. Place the auto filling cap over a container.
4. Press and press .

5. Select
6. Press to prime the e-pump and rinse the tubes.
7. Remove the priming nozzle of the outlet tubing from the demineralized water tank.
8. Press to prime the e-pump to remove the demineralized water from the tubes.
9. Disconnect the kit tubes from the MASTERCLAVE®.
10. Place the kit in an autoclave bag.
11. Autoclave at 121°C for 15 minutes.
Note: For replacement frequency of the filter, contact the filter manufacturer.
Cleaning the Auto Water Filling for Network (MASTERCLAVE® 20)

Decontaminate the auto water filling for tank tubes:
• when installing the system,
• when the demineralized water source has not been used for a long time,
• when after 1 month of using the demineralized water source,
• when changing the water source.

Cleaning the Auto Water Filling for Network (MASTERCLAVE® 20) User Maintenance
1. Disconnect the auto-filling cap from the tubing.
a)Hold the metallic ring of the auto-filling cap quick connector.

b)Pull out the tube.
2. Place the auto filling cap tube into a drip tray.
3. Press and press .
4. Select .
5. Press to prime and rinse the tubes.
6. Disconnect the demineralized water inlet tube from the demineralized water line.
7. Press to prime to remove the demineralized water from the tubes.
8. Reconnect the auto filling cap to the tube.
9. Disconnect the auto filling tube from the MASTERCLAVE®.
10. Place the auto filling tube in an autoclave bag.
11. Autoclave at 121°C for 15 minutes.
Note: For replacement frequency of the filter, if installed, contact the filter manufacturer.

Preventive Maintenance Operations User Maintenance
Preventive Maintenance Operations
Replacing Seals and Filters
Name Frequency
Priming port cap seal 16x2 (1) Every 250 cycles
Priming nozzle seal set (2) Large seal: every 90 cycles
Small seal: every 40 cycles
Priming tube connector (3) Every 90 cycles

Port cap seal for supplement addition (4) Every 250 cycles
Non-return valve seal 11x2 (5) Every 90 cycles
Air intake filter (6) Every 250 cycles
Draining grid Clean every 3 months
Water inlet filter Clean every 12 months
Stirrer axe black washer 250 cycles
Stirrer black gasket 250 cycles
Draining the Water Bath

It is recommended to drain the water bath at least weekly.
1. Press , then .
2. Press .
3. Press .

Replacing the Water Inlet and Outlet Hoses User Maintenance
4. Press .
It is possible to choose two different draining methods:
• Automatic draining when the instrument is locked and under pressure.
• Manual draining to clean the water bath and activate the water flow manually.
Replacing the Water Inlet and Outlet Hoses

If it is necessary to replace the water inlet and outlet hoses, make sure the replacement
hoses have the same specifications as those provided:
• Inside diameter: 12.7mm ±0.6mm
• Thickness: 4mm
• Temperature resistance: 90°C minimum (outlet)
Checking Sensor State

It is necessary to check the state of the instrument sensors and to check that they are
operating correctly.
1. Press , then .
2. Press , then .
3. Press .
4. Follow the instructions given in the Help feature to check the state of the sensors.
If the system displays on, the sensor is working correctly.
Fitting a Roll of Paper in the Printer
1. Switch off the instrument.

2. Open the printer safety cover.
3. Remove the used roll and fit a new one.
The paper must be facing down.
4. Close the printer safety cover.
5. Switch on the instrument.
6. Press .
7. Press .
8. Press .
9. Select the ticket printer.
10. Press .
11. Press to test the printer.

Managing the Maintenance Logs User Maintenance
Managing the Maintenance Logs
It is necessary to display and reset the maintenance logs for the instrument and send
anonymous reports to optimize their use.
1. Press , then .
2. Press and enter the administrator password.
3. Press , then .
4. If needed:
• See all maintenance logs.
• Reset User maintenance logs.
• Press to activate/deactivate the regular sending of anonymous reports to
bioMérieux®.
Authorizing Remote Control
Authorize remote maintenance to allow customer service to take control of the instrument and
troubleshoot remotely.
It is necessary to contact customer service to authorize remote control.
1. Press , then .
2. Press , then .
3. Press .
4. Follow the instructions given by the customer service.
Performing Functional Tests
It is necessary to implement simple tests (light indicators, pumps, sound, etc.) and sequential
tests on the equipment to check for malfunction.
1. Press , then .
2. Press , then .

A Appendix - Troubleshooting
Error Messages and Recovery Procedures
Error Message / Problem Resolution

Dose volume decreases during dose • The non-return valve is clogged. See
dispensing. Cleaning the Non-Return Valve.
• The priming tube connector is clogged or
leaking. Replace it.
• The filter is clogged. Replace it.
• The dispensing tubing set is pinched.
Failed sealing test. • Check that all caps are tightly screwed on
and that seals are in good condition.
• The filter is clogged. Replace it.
• The non-return valve may be clogged.
See Cleaning the Non-Return Valve.
• If the problem persists, contact Customer
Service.
The cooling phase seems to be too long or • Insufficient water inlet flow rate.
an error message indicates missing cooling
• Water inlet filter is clogged or upstream
water.
inlet tap may not be sufficiently open.
• The cycle mode selected is Normal or
Slow. They are used to save water. The
cooling phase might be longer than the
Fast mode.
Abnormal temperature variations. • Stirrer missing or in wrong position.

• Media temperature probe is damaged.
Contact Customer Service.
Bubbles in the medium when dispensing. • Check the priming nozzle seal and the
state of the priming tube connector.
Printer malfunction. • Check that printer is enabled in the

Peripherals menu.
• Paper incorrectly positioned, stuck, or
missing.
A wrong volume is dispensed in the vessel, • Perform a calibration and, if necessary, an

using the automatic filling mode. adjustment in the Metrology menu.
161150-1293 - A A-1 MASTERCLAVE® 10 MASTERCLAVE® 20

B Appendix - Third-Party Software
Third party software and any parts thereof identified in the left-hand column are subject to the
terms and conditions under which the specific licenses identified in the right-hand column are
granted.
Third-party software and parts License
Qt 4.8 http://doc.qt.io/archives/qt-4.8/licensing.html
Third-Party Licenses Used in Qt 4.8 http://doc.qt.io/archives/qt-4.8/3rdparty.html
GCC 4.8 Licence GPL-3.0 https://gcc.gnu.org/

Quazip https://github.com/stachenov/quazip
License LGPL https://github.com/stachenov/quazip/
blob/master/COPYING
libsmbclient https://www.samba.org/samba/docs/current/man-
html/libsmbclient.7.html
License GPL http://www.gnu.org/copyleft/gpl.html
libqxt License BSD3 https://bitbucket.org/libqxt/libqxt/src/

master/COPYING
zint License GPL-3.0 https://zint.github.io/
Linux License GNU GPL
U-Boot License GPL-2.0
Buildroot License GPL-2.0
161150-1293 - A B-1 MASTERCLAVE® 10 MASTERCLAVE® 20

C Appendix - Glossary
Adjustment
The process of adjusting the equipment to reduce the deviation between the measurement
made and the reference or standard volume (benchmark).
Calibration
Calibration involves comparing a measurement made by the equipment with a reference or
standard volume (benchmark).
F0
Equivalent exposure time at 121.11°C than the actual exposure time at a variable temperature
calculated for an ideal microorganism with a temperature coefficient of destruction of
10°C (Z).
LIMS
LIMS (Laboratory Information Management System), is an integrated laboratory and
information management application.
161150-1293 - A C-1 MASTERCLAVE® 10 MASTERCLAVE® 20

Revision History
This section contains a summary of changes made to each released revision of this document
starting with part number:
Change type categories
N/A Not applicable (First publication)

Correction Correction of documentation anomalies
Technical change Addition, revision and/or removal of information related to the product
Administrative Implementation of non-technical changes noticeable to the user
Notes: • Minor typographical, grammar, and formatting changes are not

included in the revision history.
• Not all versions may be available in all languages.
Release Date Part Number Change Type Change Summary

2019-10 161150-1293 - A N/A First publication
bioMérieux SA
376 Chemin de l'Orme
69280 Marcy-l’Etoile - France
Tel. 33 (0)4 78 87 20 00 Fax 33 (0)4 78 87 20 90
www.biomerieux.com

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User Manual - Original

Introduction to the System.....................................................................................1-1

System Description and Basic Operations...........................................................3-1

System Installation and Configuration................................................................. 4-1

161150-1293 - A i MASTERCLAVE® 10 MASTERCLAVE® 20

Connecting the MASTERCLAVE® 20 to a Demineralized Water Source ................... 4-9

Workflow and Instructional Procedures............................................................... 5-1

161150-1293 - A ii MASTERCLAVE® 10 MASTERCLAVE® 20

Consulting the Instrument Information.................................................................6-1

User Maintenance................................................................................................... 7-1

Appendix - Third-Party Software...........................................................................B-1

161150-1293 - A iii MASTERCLAVE® 10 MASTERCLAVE® 20

The content of this document is based on the Software release 1.0.

Intended Use and Users

Benefits and Limitations of Use

Warning and Safety Messages

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CAUTION: A Caution statement alerts the user to the possibility of a problem

IMPORTANT: An Important statement relates to content presented in the user documentation. It is

Note: A Note statement supplies additional information about a topic.

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IMPORTANT: It is important to follow all the restrictions on use, particularly concerning

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Compliance with China RoHS

Batch code Catalogue number

Serial number Consult Instructions for Use

Use by date Manufacturer

Date of manufacture This way up

Caution, consult accompanying

Contains sufficient for <n> tests Do not reuse

Keep dry Keep away from light

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Fragile, handle with care Humidity limitation

Keep away from magnetic field Temperature limitations

Upper limit of temperature Lower limit of temperature

Sterile Positive control

Negative control Biological risk

Electric shock warning Radiation warning

Hot surface Potential pinch-point warning

Laser beam Shearing hazard

High temperature Hazardous magnetic field

Potential tip-over/crush hazard Acute toxicity

Corrosives Sodium azide

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Environmentally friendly use period.

Fuse Direct current

Alternating current Both direct and alternating current

Three-phase alternating current Earth (ground) terminal

Protective conductor terminal Frame or chassis terminal

ON (only for a component of the

Equipment protected throughout by

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CAUTION: Use of this instrument in a dry environment, especially if synthetic

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Industry Canada (IC) Compliance

Industry Canada (IC) Compliance

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1 Danger - Steam release

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CAUTION: Any liquid spilled on the system may result in system