DIFFERENTIAL CELL COUNT
HEMATOLOGY
BS Medical Laboratory Science | SEM 1 2022
Stained blood film is scanned
microscopically to:
● estimate WBC count
● identify the morphologic RBC
abnormalities
● estimate the platelet number
● classify WBC into group types
Segmenters/ Neutrophils/ PMN
- 2-5 lobes with fine purple
granules all over cytoplasm
- produces proteolytic enzyme
serve as a phagocyte
- normal value: 50-70%
Monocyte
- largest cell in circulation
- bean shaped/ kidney shaped
nucleus with convolutions and
lace-like chromatin pattern
- serve as phagocyte and produces
proteolytic enzyme
- normal value: 3-8%
Lymphocyte
- has large round compact nucleus
with thin rim skyblue cytoplasm
or robin-egg blue cytoplasm
- normal value: 25-40%
Differential counter
Relative Count
- % of routine total leukocyte ct.
represented by a specific cell
type
Formula:
WBC type
RC= 100 WBC x 100
Normal Value: [Relative ct]
● Neutrophils- 47.0-79.5%
● Eosinophils- 0.0-7.5%
● Basophils- 0.0-2.0%
● Leukocytes- 12.5-40.0%
● Monocytes- 2.0-11.0%
Absolute Count
- actual number of a cell type per
liter of blood
Formula:
AC= relative ct. x total WBC ct.
Normal Value:
● Neutrophils- 1.8-7.7 (x10^9/L)
● Eosinophils- 0.0-0.45
● Leukocytes- 1.0-4.8
● Monocytes- 0.0-0.8
When 100 wbc have been identified and
recorded, it may be classified either:
a. Schilling’s classification
b. Filament and non-filament
classification
c.
Schilling’s Classification
● one of the first to notice that
many diseases is caused by an
increase in the percentage of
immature neutrophils of the
granulocytic series
DIFFERENTIAL CELL COUNT
HEMATOLOGY
BS Medical Laboratory Science | SEM 1 2022
cell percent
Example:
Neutrophilic: lymphocyte 20-35
Myelocyte: 0%
monocyte 2-6
Metamyelocyte: 0%
Band Cells: 2-6% eosinophilic 1-3
Segmented Cells: 55-75% segmented cells

basophilic 0-1
note: immature cells on the left side of segmented cells
the chart, if the percentage of these
immature cells become increased,
Filament and Non-Filament
schilling called it as “shift to the left”;
Classification
if accompanied by a low wbc count, it is
called “degenerative shift to the left”: ● reports the percentage of each

seen in cases of typhoid fever due to cell with the exception of the

depression of cell factories in the bone neutrophils of the granulocytic

marrow; if accompanied by a high wbc series

ct, it is called “regenerative shift to - which is now the main

the left”: found in infectious diseases difference between the

due to stimulus of the cell factories in Schilling’s classification

the bone marrow ● neutrophils of the granulocytic

series are reported:
mature neutrophilic
cell percent
- neutrophilic segmented cells
neutrophilic 0 [makes up the filament ct]
myelocyte immature neutrophilic
- neutrophilic myelocytes
neutrophilic 0
[non-filament ct]
metamyelocyte
- neutrophilic metamyelocytes
neutrophilic band 2-6 [non-filament ct]
cell - neutrophilic band cells
neutrophilic 55-75 [non-filament ct]
segmented cells