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Updated August 25, 2020
SDSU Extension
There has been a renewed interest in taking samples of soybean leaves for nutrient analysis as a quality control
tool to ensure soil and the fertilizer programs are meeting the needs of the plant to eliminate nutrients as a
yield limiting factor. Most soybean fields are at the correct growth stage to take a leaf sample for nutrient
analysis as highlighted in the new SDSU Extension video, How to Take a Soybean Leaf Sample.
1. Collect leaf samples when soybeans are at the R1-R2 growth stage, but prior to R3 (See "Growth Stage
Identification" below).
2. Identify and collect only the uppermost fully-developed leaf (trifoliolate) which is usually the third leaf from
the top that is dark green, do not include the petiole (Figure 1).
3. Collect 30 trifoliolates without the petiole from different plants from the area of interest and put them in a
brown paper bag to mail to a soil and plant analysis lab of choice.
R2 – Full Bloom: Open flower present at one of the two uppermost main stem nodes with a fully developed leaf.
R3 – Beginning Pod: When pods are 3/16th of an inch on one of the 4 uppermost main stem nodes with a fully
developed leaf. Nutrients begin to redistribute within the plant to developing seeds in new pods, e.g., potassium
Most soil labs also can conduct plant nutrient analysis. Per sample, the lab cost is about $34 (not including
shipping) to run for all 13 plant nutrients or about $26 dollars if chloride and molybdenum are left out (the
more common option available). Once you choose a lab and mail your sample, it should take a week or two to
get your results back. Nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S),
chloride (Cl) will be reported in percent (%) and copper (Cu), iron (Fe), manganese (Mn), zinc (Zn), boron (B),
If any of your nutrient values are below the sufficiency range (Table 1), your next soybean crop may or may not
benefit from management changes (drainage, inoculation, fertilization, variety selection, etc.). Plant nutrient
concentrations are sensitive to the environment and vary by variety. Analysis results should be used cautiously
or not at all if plant samples were taken under extreme drought conditions, after flooding, or from damaged
plants, i.e., herbicide carryover. However, a properly collected trifoliolate-leaf sample for monitoring soybean
nutrition during the growing season is an additional tool besides regular soil testing efforts to assess soil
fertility. The information is mostly useful for making future changes to your fertility program and
management, not in-season. Most importantly, soil test are needed to generate fertilizer rate recommendations
Interpretive categories
to…
(P)
(K)
(Mg)
(Mn)
(Mo)
(Al)‡
RELATED TOPICS
Soil Health, Growing Soybeans
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