Name: Exercise 2 actividad 2

Exercise 2: Skeletal Muscle Physiology: Activity 2: The Effect of Stimulus Voltage on Skeletal Muscle Contraction Lab
Report

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1. Skeletal muscle fibers are innervated (stimulated) by

You correctly answered: c. motor neurons.

2. A single action potential propagating down a motor axon results in

You correctly answered: d. a single action potential and a single contractile event in the muscle fibers it innervates.

3. In resting skeletal muscle, calcium is stored in

You correctly answered: c. the sarcoplasmic reticulum.

4. During the latent period for an isometric contraction

You correctly answered: c. the cellular events involved in excitation-contraction coupling occur.

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Experiment Results
Predict Question:
Predict Question: As the stimulus voltage is increased from 1.0 volt up to 10 volts, what will happen to the amount of active
force generated with each stimulus?
Your answer : b. The active force will not increase above the value observed when 1.0 volt is applied.

Stop & Think Questions:

What do you see in the active force display when the stimulus voltage is set to 0.0, and why does this observation make
sense?
You correctly answered: a. 0.00 g; there was no activation of skeletal muscle fibers by this stimulus.

What is the lowest stimulus voltage that induces active force in the skeletal muscle?
You correctly answered: b. threshold voltage

6. Enter the threshold voltage for this experiment in the field below and then click Submit Data to record your answer in the
lab report
You answered: 0 volts

12. Enter the maximal voltage for this experiment in the field below and then click Submit Data to record your answer in the
lab report.
You answered: 1.82 volts

Experiment Data:

Voltage Length Active Force Passive Force Total Force

0.0 75 0.0 0.0 0.0
0.2 75 0.0 0.0 0.0
0.1 75 0.0 0.0 0.0
0.1 75 0.0 0.0 0.0
0.5 75 0.0 0.0 0.0
0.8 75 0.02 0.0 0.02
1.0 75 0.15 0.0 0.15
1.5 75 0.43 0.0 0.43
2.0 75 0.66 0.0 0.66
2.5 75 0.87 0.0 0.87
3.0 75 1.04 0.0 1.04
3.5 75 1.19 0.0 1.19
4.0 75 1.32 0.0 1.32
4.5 75 1.42 0.0 1.42
5.0 75 1.51 0.0 1.51
5.5 75 1.59 0.0 1.59
6.0 75 1.65 0.0 1.65
6.5 75 1.70 0.0 1.70
7.0 75 1.74 0.0 1.74
7.5 75 1.78 0.0 1.78
8.0 75 1.81 0.0 1.81
8.5 75 1.82 0.0 1.82
9.0 75 1.82 0.0 1.82

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Voltage Length Active Force Passive Force Total Force
9.5 75 1.82 0.0 1.82
10.0 75 1.82 0.0 1.82

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Post-lab Quiz Results
You scored 100% by answering 6 out of 6 questions correctly.

1. Motor unit recruitment refers to

You correctly answered: a. an increase in the number of active muscle fibers to increase the force developed in a muscle.

2. Active tension (or force) in a skeletal muscle fiber results from

You correctly answered: a. activation of cross bridge cycling via increased intracellular calcium levels.

3. The ________ is the minimal stimulus needed to cause a depolarization of the muscle plasma membrane (sarcolemma).

You correctly answered: d. threshold voltage

4. By definition, the ________ is the amount of stimulus required to successfully recruit all the muscle fibers into
developing active force.
You correctly answered: c. maximal voltage

5. Why was a maximal voltage observed in this experiment?

You correctly answered: b. At the maximal voltage, all the muscle fibers contained in this muscle are depolarized and they
all develop active force (that is, they were all successfully recruited).

6. A sufficiently strong electrical stimulus applied to an isolated, mounted skeletal muscle induces the development of
muscle force, or muscle tension. Which of the following statements concerning this observation is true?
You correctly answered: c. The electrical stimulus mimics acetylcholine release at a neuromuscular junction

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Review Sheet Results
1. Describe the effect of increasing stimulus voltage on isolated skeletal muscle. Specifically, what happened to the muscle
force generated with stronger electrical stimulations and why did this change occur? How well did the results compare with
your prediction?
Your answer:
El aumento del voltaje del estímulo que se da sobre el el músculo esquelético permite que la totalidad de la fuerza
aumente. Como se esperaba, la fuerza activa aumentó de manera continua, pero cuando el voltaje máximo se redujo, este
no aumentó.

2. How is this change in whole-muscle force achieved in vivo?

Your answer:
El cambio que se produjo en la fuerza total del músculo se logró in vivo donde se reclutan unidades motrices adicionales
para aumentar el total de la fuerza muscular producida. El voltaje va sobre el umbral, este crea una fuerza entera del
músculo, cada vez más la fuerza se fortalece con la mayor cantidad de estímulo hasta alcanzar al máximo

3. What happened in the isolated skeletal muscle when the maximal voltage was applied?
Your answer:
Todas las ?bras musculares en el músculo esquelético se despolarizan y todas producen una fuerza activa. Toda esta ?bra
participó en producir el voltaje máximo.

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