11/30/21, 5:40 PM PhysioEx Exercise 2 Activity 2

PhysioEx Lab Report

Exercise 2: Skeletal Muscle Physiology

Activity 2: The Effect of Stimulus Voltage on Skeletal Muscle Contraction

Name: ПАВЕЛ МИЛЕНКОВСКИ

Date: 30 November 2021

Session ID: session-e4c57de0-0af1-e5d7-54f6-b939dd62417a

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 Skeletal muscle fibers are innervated (stimulated) by

You correctly answered: motor neurons.

2 A single action potential propagating down a motor axon results in

You correctly answered: a single action potential and a single contractile event in the muscle
fibers it innervates.

3 In resting skeletal muscle, calcium is stored in

You correctly answered: the sarcoplasmic reticulum.

4 During the latent period for an isometric contraction

You correctly answered: the cellular events involved in excitation-contraction coupling occur.

Experiment Results
Predict Question
1 Predict Question: As the stimulus voltage is increased from 1.0 volt up to 10 volts, what will
happen to the amount of active force generated with each stimulus?

Your answer: The active force will continually increase.

Stop & Think Questions

1 What do you observe in the active force display when the stimulus voltage is set to 0.0, and
why does this observation make sense?

You correctly answered: 0.00; there was no activation of skeletal muscle fibers by this
stimulus.

2 What is the lowest stimulus voltage that induces active force in the skeletal muscle?

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You correctly answered: threshold voltage.

3 Enter the threshold voltage for this experiment.

You answered: 0.8 volts.

4 Enter the maximal voltage for this experiment.

You answered: 8.5 volts.

Experiment Data
Voltage (v) Length (mm) Active Force Passive Force Total Force
0.0 75 0.00 0.00 0.00
0.2 75 0.00 0.00 0.00
0.3 75 0.00 0.00 0.00
0.9 75 0.09 0.00 0.09
0.8 75 0.02 0.00 0.02
1.0 75 0.15 0.00 0.15
1.5 75 0.43 0.00 0.43
2.0 75 0.66 0.00 0.66
2.5 75 0.87 0.00 0.87
3.0 75 1.04 0.00 1.04
3.5 75 1.32 0.00 0.06
4.0 75 1.32 0.00 1.32
4.5 75 1.42 0.00 1.42
5.0 75 1.51 0.00 1.51
5.5 75 1.59 0.00 1.59
6.0 75 1.65 0.00 1.65
6.5 75 1.70 0.00 1.70
7.0 75 1.74 0.00 1.74
7.5 75 1.78 0.00 1.78
8.0 75 1.81 0.00 1.81
8.5 75 1.82 0.00 1.82
9.0 75 1.82 0.00 1.82
9.5 75 1.82 0.00 1.82
10.0 75 1.82 0.00 1.82

Voltage = 0.0 V

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Voltage = 0.2 V

Voltage = 0.3 V

Voltage = 0.9 V

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Voltage = 0.8 V

Voltage = 1.0 V

Voltage = 1.5 V

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Voltage = 2.0 V

Voltage = 2.5 V

Voltage = 3.0 V

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Voltage = 3.5 V

Voltage = 4.0 V

Voltage = 4.5 V

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Voltage = 5.0 V

Voltage = 5.5 V

Voltage = 6.0 V

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Voltage = 6.5 V

Voltage = 7.0 V

Voltage = 7.5 V

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Voltage = 8.0 V

Voltage = 8.5 V

Voltage = 9.0 V

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Voltage = 9.5 V

Voltage = 10.0 V

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Post-lab Quiz Results

You scored 83% by answering 5 out of 6 questions correctly.

1 Motor unit recruitment refers to

Your answer: a decrease in the number of active muscle fibers to increase the simplicity of
force development in a muscle.

Correct answer: an increase in the number of active muscle fibers to increase the force
developed in a muscle.

2 Active tension (or force) in a skeletal muscle fiber results from

You correctly answered: activation of cross bridge cycling via increased intracellular calcium
levels.

3 The is the minimal stimulus needed to cause a depolarization of the muscle plasma
membrane (sarcolemma).

You correctly answered: threshold voltage.

4 By definition, the is the amount of stimulus required to successfully recruit all the
muscle fibers into developing active force.

You correctly answered: maximal voltage.

5 Why was a maximal voltage observed in this experiment?

You correctly answered: At the maximal voltage, all the muscle fibers contained in this
muscle are depolarized and they all develop active force (that is, they were all successfully
recruited).
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6 A sufficiently strong electrical stimulus applied to an isolated, mounted skeletal muscle

induces the development of muscle force, or muscle tension. Which of the following
statements concerning this observation is true?

You correctly answered: The electrical stimulus mimics acetylcholine release at a

neuromuscular junction.

Review Sheet Results

1 Describe the effect of increasing stimulus voltage on isolated skeletal muscle. Specifically,
what happened to the muscle force generated with stronger electrical stimulations and why
did this change occur? How well did the results compare with your prediction?

Your answer:

Вкупната сила на мускулот се зголемува кога се зголемува напонот на дразбата. Моите

предвидувања беа точни со тоа што силата постојано ќе се зголемува. Активната сила
се зголеми на 8,5 волти.

2 How is this change in whole-muscle force achieved in vivo?

Your answer:

Силата на целиот мускул се постигнува in vivo бидејќи поради тоа што се користеа
дополнителни моторни единици како зголемување на силата произведена од
вкупниот мускул.

3 What happened in the isolated skeletal muscle when the maximal voltage was applied?

Your answer:

Влакната во изолираните скелетни мускули беа деполаризирани и се создаде активна

сила. Максималниот напон беше применет кога учествуваа сите влакна во скелетните
мускули.

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