St f live: argomivms ov enreynee Fron. argrrinms |e produce. procucis ome processes useful to WumOMs; Tasery Biotectopdegys: r : : Zn vitro Jertit ization. leacting toa Clent—tabe! baby, Spmrbeniat ogee ovnd using jt, Level sping @ ONA Vaccine. a correcting a. defective qeme, ove aL pot yy bioteshmology { ae | (The. bretegraction. Ot matural sgelomte, ovnd or Dine eetlat pairs Frere of ord Mmolecularr oma loguan for predicts and serviezs, PRINCIPLES OF O20 TEC UNOLOMY ., OGenstic Engl neering Tethvigues +e atter recs ob emetic material CONA amd RIVA) sto introduce. inde ot orgornionns evnd hun chagge. tue. pheno type ob the host jovndarn, - @ Biopress engineering maintenance. o. ile, Confer auf eortamination —frex) oymbience in Ehemical on paar Procereer +o enable. quer. ob one tie cerired rl croke | eukor yoHe cel in Longe quomtttien ter tus, momnufactute. of bloteehnoteg ical precluets, Like, omti bioHer — vaccine -€ pan ete, eyou probably appreciate ts. acvaregen ok sexual reproduction over Ovenil reprocluetion . Astcual’ reprecluction preservér tha emetic information, while sexttal Yepreduction pemmits varioction, “t soo { ) (r - Zoom: / ona. ‘ y \ baud DA. : onmid’) : _7 tnolation ob roe ip) benstic (Eto) — —P@ermntel_ Material Ploninidl emt ot I . ae nterert Ligant” (Moleutorr glse) Ppgems. ob Interent| [Recorabin | Bloomia i we zi erarrnerct) 4 Cstomler Cohen ord [erbert Rayeraccamplished tis Hn ez) cout a Geren 3 By Fgphi nuuriuny (7 Higa Cale qe iD brig Resistor Plomnld) [4st —Recombinanct Plasmid J TRecombinomt Ploamid] [rem ternation in B. cobi Brug Reactant gene NAL Pore fon mnt & calf ttt, rag You com henee. in med fying om. org ortiem — © saentl fication Vet DUA with denirable gemsns Introduction of the ident feel pa into the host UD mointenance. ofein troduced DIVA repintart] fer tat twere. are. -tyicen. tonic steps in genatioly é 12 hertamd transfer Of tux DNA +o its progeny, Oona erewalputeting, Enzymons L Fuck © Restriction Em; Crolgenlorm seirrorsy7 y ° Polumeraee. em mano [Uatd- type © rae hey | # Lg oveee( molecitom que) PNA polymerase) Meter “— ® vecturs (moleeidom vehicky 2 8 | era Ofer exgeenioyn) l Ty Li ¢ ibs Naot, ieee haoladiin Laem PhEspho- eater bond Ty meterophage,* The Hirst true” restriction emdopueteacss ion tolated 141990. by \Nctinaris! ormd) -Hemnl Hen Sinétel, (4rem = Hestmnepvitts ange naa) “dl te selemis were urd tea, Nol Pets somd Medicine in 4948) Yor tne. Leseove ers ot endonicteanes, GJ bactevlephage—virw.| @ : i . ! Bacteria) ! Theo, Qo view, H aes { | Se | OF eaeat (nie icterpleted Gms) iy . , | be ' é . sInethe year $063, tle Hiss bmzymers Terponsibie, tor rootnledin, tue g701 oF bactenlophage. im © Escherichia cou were holakh, OME of ttre aokled 16 “to. DNA ), while tee ieee cae ee Te tae ee seer fr * Reatrietion e raymen betong-to a tare ger clasn ok enaymen cotlad | *Weleaces. Thea. vre. a hod Virdee x3 : : Na Deron ctocas 9 LE ccommnccoh ,, Pmepprerten band “EP2s bohdy: | Gz | | @ Endonvetooee—> peeceepepoOcO ATP vi | stg [POR ceagrisation | summits : | 1 ay Qietvepletion- cut tee DNA ot | Bent tur NA | -Speeitio: site, Skandomby (PTS vortrietion endonuclease >Hind It (Type-s2) whose | tenctioni clepended on a specitie DNA yun ida. 7 Sequence | Wa inoladea (Hamilton Smith) omd characterteed five: years later, | TE wor found-taat Hind TL alvoays exct DNA molecules I act o TovEHeuLlore polret- bye tag a specitic sequgnte. ot 51k howe Paks. Ths, Specitic. worse Kor. 4 knvun Ove tua vecog nition aquane, por Hind ay | |7 wictin : f Gh ; AcY Ac 3! Teh &T (Ops iynbcine. Tor ¢) (POD oo 3 CA (hime. Aor & (opis dine Tore) TE, — st S'— ariaac—3! 8 —@aaltt o—s! (eat) 900 wwatviction jPBericten Hind sr, 2 tein pee oe awver 230 Strains Pd teat he been Pole cliffererrt ese tog o& “bacteria cach of whet eee Seqjttances, a | | Palindromic. : ge, uncer ax specitie Podindran, (Bach wextvietin endomucteante sccoquise? MWeleoticle. cequiencen iv—fuie. DNAY “MALAYALAM” pero bowe. palys Sequences. | ——* E9>[Ecors | 8'—— Glaatte — —a! s——ettaa | 3! (bare, pair Sequence) sl Nomencloctune, of Yeatriction enzymes leFist iether ob tus, name gent, witch, |* Second two letters — Species of tue prokaryotic. eeu frm 4 : cer were. holated #99 [EcoRT] comen cicoetees (37 BeoRZ, tae t letter (Rh oterived From tad il Romane members ~ order in Wohich “te, emaymes ‘were Nroloctect from. trod. peed Stain of bacteri, ES adem ob zanlation, ittine rab, Eo orter ob facta, Heme inbluenae, ye 8. edie lisiomti, ch sane ttn Spegey StromEnayr Mierdoial Origin [eam HI [Bacittine omploliquaaciens [peo RE Escheriehla coli hind Tr | Pst brovidercia #iuariiy | [az am sod A sol [sreptomyces al bun ens’ , | Si-oktae=G— ~A=e=at | tla “marvenkh, 3iac-A =o ii Smat [Senrds A nareoncens C7 A-b —c THO Is erence © fame a, 39 -G~ Drug Real we Selectn ble | Marker Vector rr AR Resteletion ste. 04 Hind —3r = Recombinant meena | SECT ARLE MARKER: | Eg Antiblotin eatstanee. gens, ' : Sheek gens or B—golactosidone. ergy me. (produces, colour in tee Preaenth. ob a ehvernagemic, sabatan CT substraste), _> Non Reepimbi nantt “trated hervanvavet . beacon 7 > Recombinant Trantor 2 Cloning silent palindlrsrnic Cequencen (reatviction sie q~g mucteotisy) Eee with yn selectable. "marker, > pry Renivtomt genes gicctabl. mon kore on at Lac. gen _o(int within, s:lectable. marker),‘Total ne.ob Restriation ¢iteare * Nob © Restriction gtertnt in PBR 222 sh i Stleeta ile More pow . &= Bolivenr # R=Rodeiquez ae Selection of recombinants ovnd transhormomt — bowed on Seleat Hc aan ers owlace gone. inactivation pyomiibiot te. VWketvadion | Sy Er selectable Marler a ud, Restart, geal Seat a Lee. 27% qone (meek sional Restriction, Stren ae ee ena ob ast LF RE+ Seqmont ob DNA+ Ligowe. ae tet ® Functional) 4 + \ # Apter Tnsevtiona) {x Teer [er oNA res Gene ot Ditevent (DNA) [Beata bho» Aniibiotic. Reabstant Genes j Boros) 5g eston Ls 3 moet wort) 1 > dees ie | Se e@T oO RECBame a “Te parle Low “big ere : ‘ [Peete " Nan Reccrnisinctit TPeemsbormannct; Recombinamt \ Teovstermavt: Tarleton oy Reena Qithaut Arti blot =o Non Pram formant] ® (HetR ayn? .e a63 Meee eT Sachse | Blan Clear Nata reaeo ramps aunt biotic eo nic foe Subspanee Plants Agrobacterium “untifaciens. a pathogen of several'dtcot Plants tp able todeliver a piece DNA: lena ONAP to! Jroneform normal plot: coe into aatwmor omd divert thsat : jurmnar colts fo proauca_ Tue chemical required by tus pathogen, oes temmane"rattscing TH) plead) 2b, Agrdbaclevinan, tesecens * hon now bec rod iTa info 2 Cloning vector whch tno move fatthegemic. to-tna plomts but iP eau “otble-to Use, tue ynechantam tr daliver qones pow our intexeat nto a wet CE arent Dh 0NA es igor (ein, | S Conia Oto al weligoteeL DA igo fPekin 1 DSRNAFe qrercation. “ow DNA by entation erdonucleaacn , Rentriction Enlonucionse) agen. ob Interent Dseporetior ek ovnd. 3p Section sak Ateaive WA ceopment by gpl clectrophoresh | yBoleerslare gene eee

b'-0H ) wa peimon > provi + psec] cee tAgjer Portion fem NRT Reach NCERT a