Assy Carry out for the method for high performance liquid chromatography (0512) Chromatographic system and system suitability — Use ‘octadeeylsilane bonded silica gel es the stationary phase and mixture of acetonitrile nd 0.05 mol/L. potassium dihyelrogen phosphate (50 #50) (4 Sodium Dodecyl sulfate per 100 ml of stationary phosey adjust pH to 4.0. with phosphoric acid) as the mobile phase. As detector a spectrophotometer set ct 345 nm. The number of theoretical plates of column i€ not less than 5000. caleulnted with the reference to the peak of berberine hydrochloride. Reference solution hydrochloride CRS, solution containing 80 ax of berberine hydrochloride per mal ts the reference solution. Weigh accurately a quantity of berberine fissalve in methanol to produce a Test solution To 0.25 g of powder, sceurately weighed. in 4a stopper conical flasks add accurately 25 ml of mixture of hydrochloric acid and methanol (1 1009+ weighs beat tunder reflux at 85% for 40 minutes. Cool and weigh agains replenish the loss of stcigh with » mixture of hydrochloric scid and methanol (1 #100)+ mix wells centeifges filter the supernatant. use the suceessfal filtrate as the test solution. Procedure Inject accurately 5 yl of each of the reference solution and the test solution into the column, respectively taleulate the contents, It contains not less 2.0 mg of berberine bydrochloride (Cy Hy NO, * HCD por gram, referred to Phellodendet Chinensis Cortex Actions To clear heat snd dry dampness. Indications Bi disorders due to dampnesshest pouring downward, manifested as reddened. swollen and painfal feet and knees. heaviness ir the lower limbs» yellow and seanty Administration and Dosage For oral sdministeation, 6-9 per times twice oF three times dy. Precautions and Warnings Use with caution du 1 pregnancy: Storage Preserve in vihily elosed containers, Sangi Pian (th) Sapai Tablets Ingredients Notoginseag Radix et Rhizoma 100 g, Procedure Pulverize Notoginseng Radix ot Rhizoma 10 fine powder. add a quantity of exceipient. make geanules. compress into 1000 tabietsy or cout with fla hig tablets ‘or compress into 2000 tablets (small tabl Description Greyish-ysllow to brownish-yellow tsbletss or film coated tablets with greyist-yellow to lrownish-yellow tastes bitter snd slightly sweet Mentification C1) Microscopieals Fragments of resin canals containing yellow secretion « Notoginseng Radix et Rhizoma). (2) Macerate 0.5 of powders in 10 ml of methanol by hoking for 0 minutes. filters evaporate the filtrate to and dissolve the residue in 1 al of methanol as the test solution, Dissolve notoginsenoside Ry CRS+ ginsenoside Rb, CRS nd ginsenoside Ry CRS in methanol 10 produce a rmixture containing 0.5 mg of each per ml as the reference solution. Carry out the method for thin layer chrome dryness tography (0502), using silica gel G as the costing substance and a mixture of chloroform. methanol and water (7 = 3 0.5) as the mobile phase. Apply separately 5 pl of each of the tow0 solutions to the plate, After developing anil removal of the plate. dey in air, Spray with « 10% solfure acid in ethanol and Theat at 105°C to the spots clear, Examine in daylight the spots inthe chromatogram obtined with the fest solution correspond in postion and colour to the spots in the chromatogram obtained with the reference solution, Examine ander ultzaviolet light at 365 am, the fluorescent spors in the chromatogram obtained with the test solution correspond in position and colour to the spots in the chromatogram obtained with the reference solution, ‘Other requirements for tablets (0101). Assay Carry out the method for high performance liquid chromatography 0512). Comply with the general requirements Chromaiogra phic detadecylsilane bonded silica gel as the stationary phases acetonitrile as the mobile phase Ay water as the mobile phase B, elute in gradient as the following. system and syntem suitabitiry — Use Time Mobile phase A Mobile phase B Gminutes) (per eent V/V) (per cont V/V) paz 19 8 12-60 19-36 roa As detector a speetruphotometer set at 203 nm. The number of theoretical plates of the column ie not less thin 4000, caleulated with the reference to the pek of notogin senoside R, Reference solution Dissolve a quantity of gintenoside Rg, CRS. ginsenoside Rb, CRS. and notoginsenoside Ry CRS. weighed accurately. in methanol to produce a mixture solution containing 0.4 mg, 0.4 mg. and 0.1 mg per ml respectively as the reference solution Take 10 tablets» grind imo fine powder. weigh accurately 0.8 g in a stopper conical flasky avd 50-ml of methanol. weigh, allow to stand overnights heat unser reflux, fon a water hath at 80°C for 2 hours, cooly weigh again, Test solution replenish the loss of seeight with methanols shake sells fier tnd use the successive filtrate as the test solution, Procedure Inject aceurately 10 y of the reference solution tnd the test solution into the columns respectively. and caleulate the content, 1k contains ot less than 10,0 mg of yer small tablets or 20.0 my of por big tablet» of the total amount of ginsenoside Ray (Cy Hye Oy) ginsenoside Rb) (Cy Hyy On) and rotogin-senoside Ry (Cir Hy» Ou)+ refereed to Notoginseng, Radix e¢ Rhizome, Actions To dissipate stasis. swellings and relieve pain stop bleeding. disperse Indications Hemoptysis. hematemesiss epitaxis. bloody. stool, mensteual flooding and spotting traumatic bleeding abdoroinal pains swelling and pain due to traumatie injuries. Administration and Dosage For oral administrations « 12 pills or 2-5 large pills per timey three times a day. Precautions and Warnings Contisindiated during preguaney. Strength (1) 0.25 g of erude drug per small tablet (2) 0.8 g of erude drug per big tablet Storage Preserve in tightly closed containers