Seat No.: ________ Enrolment No.

___________

GUJARAT TECHNOLOGICAL UNIVERSITY

BE - SEMESTER–VII (NEW) EXAMINATION – WINTER 2021
Subject Code:3170414 Date:29/12/2021
Subject Name:Tissue culture
Time:10:30 AM TO 01:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
4. Simple and non-programmable scientific calculators are allowed.

MARKS
Q.1 (a) Rajabhai is using DMEM with 10% BSA for growth of MCF7 cell 03
lines. He found out that the growth of these cells is slower than
usual, and many cultures were undergoing cell death.
(b) If you do not use a carbon dioxide incubator, what media 04
component can you add to the animal cell culture medium so that
you can compensate for the absence of the CO2 incubator?
(c) Write a note on immature embryo culture. 07

Q.2 (a) What is the difference between somatic and gametic 03

embryogenesis.
(b) Write notes on Auxins, Cytokinins, Ethylene, Gibberellic acid. 04
(c) Ellaborate on the technologies that you can use for introducing 07
foreign genes into animal cells.
OR
(c) Ellaborate on the method used for development of primary culture, 07
and converting the same to a suspension culture.
Q.3 (a) Write a note on pH of medium and light requirement in plant 03
tissue culture. Which media components are filter sterilized and
why?
(b) “You may use any explant for regenerating a plant, but you cannot 04
make any organ from a primary animal cell culture”. Explain this
statement.
(c) Explain the use of HAT selection medium for production of 07
immunoglobulins.
OR
Q.3 (a) Write a note on the hayflick limit. 03
(b) Draw a diagram of a plant tissue culture laboratory ( well planned, 04
labeled, with measurements.)
(c) Bhauji Ranade has a 5 acre banana plantation. He recently had a 07
huge loss in yield (banana decreased, but plants are alive) due to a
virus outbreak. Now he wants to replant the same banana variety
in his farm. Ellaborate on the method he can use to obtain the same
Q.4 (a) Discuss methods that need to be followed to avoid cross 03
contamination in an animal cell culture laboratory.
(b) Differentiate between the plan of an animal tissue culture lab and 04
a plant tissue culture lab.
(c) What are the different methods you can use to introduce a 07
transgene (foreign gene) into a plant cell?
OR
Q.4 (a) Explain wet heat sterilization? Where are HEPA filters used? 03
1
 (b) Explain the reasons behind somaclonal variations and androclonal 04
variations.
(c) How can you use bioreactors for large scale production of 07
secondary metabolites? Ellaborate on the different types of plant
material that can be grown in such bioreactors.
Q.5 (a) Explain the principle of UV sterilization and Gamma sterilization. 03
(b) Explain the need for surface modification for growth of animal 04
cell culture, with examples.
(c) Elaborate about edible vaccines or plantibodies, why are they 07
lucrative compared to conventional vaccines and methods
involved in its production.
OR
Q.5 (a) What are the different methods used for sterilizing nutrient 03
media and glassware?
(b) Draw a diagram explaining the process of cryopreservation of 04
animal cell cultures.
(c) Explain various methods that can be used for creating virus 07
resistant plants.

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