Introduction

Research Identity/Academic Background

Course Outlines
Course Title
Course Code
Course outlines
Recommended Books

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 Dr. Huusain Ullah Lect-01
Ph.D Chemistry
Date: 17/10/022
❖ Ph.D Specialization: Inorganic/Analytical Chemistry
• Quaid-i-Azam University, Islamabad, Pakistan,
• Trinity College Dublin, Ireland
Research Identity: Organotin(IV) Chemistry of Hydrazones/Schiff’s Bases, MOF’s for CO2 adsorption, Water sampling and wastewater
treatment for the removal of Heavy metal using dithiocarbamate/thiocarbamate ligands and nanoparticles. Chemical and Biological Sensor,
Dye degradation and water splitting.
Research goal: To work n a creative and knowledgeable environment in the field of Inorganic/Analytical Chemistry and environmental
chemistry.
Research Projects Won: 1. Research work on an Atomic Absorption Spectrophotometer funded by the Higher Education Commission of
Pakistan, January 2011 and completed.
2. Synthesis and Characterization of Dithiocarbamate Ligands and its Environmental and Biological applications
Professional web links:
 https://www.researchgate.net/profile/Dr_Ullah2

 https://scholar.google.com.pk/citations?user=8xv_kcoAAAAJ&hl=en

 https://orcid.org/0000-0002-8349-1655

 https://www.linkedin.com/in/dr-hussain-ullah-ph-d-in-inorganic-analytical-chemistry-7725256b/?trk=public_profile_samename-
profile&originalSubdomain=pk
TITLE OF THE COURSE : ANALYTICAL CHEMISTRY PAPER: III

Code: CHEM 4012

Cr.Hrs : 03

ADVANCE SEPARATION TECHNIQUES

OBJECTIVES

Students will acquire sound knowledge regarding;

1. Advanced chromatographic techniques namely GLC, HPLC and capillary
electrophoresis
2. Applications in different fields such as food,
pharmaceuticals, petroleum, environmental and other industrial sectors.

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 ADVANCE SEPARATION TECHNIQUE
Separation techniques are those techniques that are used for the separation, purification,
identification and quantification of different compounds.
These techniques involved
1. Filtration
2. Evaporation
3. Crystallization
4. Distillation
5. Chromatography
6. Capillary electrophoresis

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 Course outlines

Introduction:
Classifications of chromatographic techniques, chromatographic processes, rate theory of
chromatography, Van-Deemter equation and its significance in evaluating column
efficiency
Gas Liquid Chromatography:
The general principle, sample preparation/derivatization, separation process, and
instrumental aspects and its applications.
 Course outlines Cont…

HPLC:
The general principle, sample preparation, separation process (normal phase and
reverse phase separation), instrumentation, method development and applications.
Capillary electrophoresis:
Theory and principle of CE, mobility, electro-osmotic flow separation by CE,
instrumentation, modes of operation, applications.

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 Recommended Books
1. Skoog, D. A., West, P. M., Holler, F. J. and Crouch, S. R., Fundamentals of Analytical
Chemistry, 9th ed., Cengage Learning, (2013).
2. Christian, G. D., Analytical Chemistry, 6th ed., John-Wiley & Sons, New York, (2004).
3. Kealey, D. and Haines, P. J., BIOS Instant Notes in Analytical Chemistry, 1 st ed., Taylor
& Francis, (2002).
4. Sharma, B.K. Instrumental Methods of Chemical Analysis, 24th ed., Goel Publishing
House, Meerut, India, (2005).
5. Grob, R. L., Eugene, F. Barry, Modern Practice of Gas Chromatography, 4th ed., John-
Wiley & Sons, USA, (2004).

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6. Kellner, R., Mermet, J-. M., Otto, M., Valcarcel, M. and Widmer, H. M., Analytical Chemistry: A
Modern Approach to Analytical Science, WileyVCH, (2004).
7. Meyer, V. R., Practical High-Performance Liquid Chromatography, 5th ed., John-Wiley & Sons,
Ltd., (201 0).
8. Lindsay, S., High Performance Liquid Chromatography, 2nd ed., JohnWiley & Sons, Ltd.,
(1992).
9. Braitwaite, A. and Smith, F. J., Chromatographic Methods, 5th ed., Kluwer Academic Publishers,
(1999).
10. Miller, J. M., Chromatography: Concepts and Contrasts, 2nd ed., JohnWiley & Sons, Inc.,
(2005).
11. Camilleri, P., Capillary Electrophoresis: Theory and Practice, 2nd ed., CRC Press, (1998)

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