Course Handout

Name of the school/college / Chitkara College of Pharmacy/ Pharmaceutical Chemistry

department offering the
course
Title of the course Advanced Instrumentation Techniques
Academic Year 2023-2024 Semester – January to June
Course code BP 811ET Course Credits 04 + 04
Name of the course Dr. Anju Goyal
coordinator
Names of other members of N.A
the team who will be engaged
in teaching the course
Lecture/Tutorial (Per Week) 08

1. Any pre-requisites for the course:

2. Scope and objective of the course:

 To understand the advanced instruments used and its applications in drug analysis.
 To understand the chromatographic separation and analysis of drugs.
 To understand the calibration of various analytical instruments and know analysis of drugs
using various analytical instruments.

3. Course learning outcomes:

(i) The basic theoretical knowledge and structural interpretation of organic and natural compounds
by NMR and Mass Spectroscopy.
(ii) Theoretically understand the aspects thermal methods of analysis and X-ray diffraction.
(iii) To understand the calibration of various analytical instruments and know analysis of drugs using
various analytical instruments.
(iv) Practical skills for the analysis of drugs and excipients using various instrumentation techniques.
(v) To understand the extraction techniques and hyphenated techniques.
(vi) To learn general theoretical principles and applications of Radio Immunological Assays.
4. Mapping of Course Learning Outcomes (CLOs) with Program Outcomes (PO):
PO CLO CLO 1 CLO 2 CLO 3 CLO 4 CLO 5 CLO6

PO1
H M
PO2
H M
PO3
M M L
PO4
H M
PO5

PO6
H M L

L: Low, M: Medium and H: High

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5. List of text books:

S. No. Name of text books

1
[T1] A.I. Vogel, J. Mendham and R.C. Denney, Text book of Quantitative Chemical Analysis, 6th ed.
Longman Scientific & technical, New York, 2009.
2
[T2] B.K Sharma , Instrumental Methods of Chemical Analysis, Latest ed, Krishna Prakashan Media,
1981.
3
[T3] R. M. Silverstein, Spectrophotometric identification of Organic Compounds, 8th ed, Wley, 2014
4
[T4] A.H. Beckett and J.B. Stenlake , Practical Pharmaceutical Chemistry, 4 th ed, The Athlone Press,
University of London, Gower Street, London, 2001.
5
[T5] D.A. Skoog, F.J. Holler and S.R. Crouch, Principles of Instrumental Analysis, 7th ed, Cenage
Learning, 2016

6. List of reference books:

S. No. Name of reference books
Provide complete details of the book as per below format. Please make sure that, the title of the mentioned Text and reference books are
same as those mentioned in Academic Program guide
1
[R1] A.I. Vogel, J. Mendham and R.C. Denney, Text book of Quantitative Chemical Analysis, 6th ed.
Longman Scientific & technical, New York, 2009.
2
[R2] R. M. Silverstein, Spectrophotometric identification of Organic Compounds, 8th ed, Wley, 2014.
3
[R3] A.H. Beckett and J.B. Stenlake , Practical Pharmaceutical Chemistry, 4 th ed, The Athlone Press,
University of London, Gower Street, London, 2001.

7. Important website links which may be referred for relevant online course resources
S. No. Website links

1
www.pharmaguideline.com

2
https://cmjournal.biomedcentral.com

3
https://www.sciencedirect.com › topics › materials-science › thermal-analysis

4
https://en.wikipedia.org

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8. Complete course coverage plan:

Lect. No. Topics Recommended

(Hour) Book
07 Nuclear Magnetic Resonance spectroscopy: Principles of H-NMR and C-NMR, T3
chemical shift, factors affecting chemical shift, coupling constant, Spin - spin
coupling, relaxation, instrumentation and applications
09 Mass Spectrometry: Principles, Fragmentation, Ionization techniques –
T2
Electron impact, chemical-ionization, MALDI, FAB, Analyzers-Time of flight and
Quadrupole, instrumentation, applications.
08 Thermal Methods of Analysis: Principles, instrumentation and applications of
T5
ThermogravimetricAnalysis (TGA), Differential Thermal Analysis (DTA),
Differential Scanning Calorimetry (DSC).
X-Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, X- ray T5
07 Crystallography, rotating crystal technique, single crystal diffraction, powder
diffraction, structural elucidation and applications.
08 Calibration and validation-as per ICH and USFDA guidelines: Calibration of R3
following Instruments; Electronic balance, UV-Visible spectrophotometer, IR
spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC.
03 Radio immune assay: Importance, various components, Principle, different
R1
methods, Limitation and Applications of Radio immuno assay.
05 Extraction techniques: General principle and procedure involved in the solid
R3
phase extraction and liquid-liquid extraction.
Hyphenated techniques: LC-MS/MS, GC-MS/MS, HPTLC-MS. R3
07
*ST1 (Syllabus Covered till date or as decided by Course Coordinator with due approval
from Dean of the school)
Nuclear Magnetic Resonance spectroscopy: Principles of H-NMR and C- T1
1 NMR, chemical shift, factors affecting chemical shift, coupling constant,
Spin - spin coupling, relaxation, instrumentation and applications
Mass Spectrometry: Principles, Fragmentation, Ionization techniques –
2 T2
Electron impact, chemical-ionization, MALDI, FAB, Analyzers-Time of flight
and Quadrupole, instrumentation, applications.
Extraction techniques: General principle and procedure involved in the
3 R3
solid phase extraction and liquid-liquid extraction.
*ST2 (Syllabus Covered till date or as decided by Course Coordinator with due approval from
Dean of the school)
1. Thermal Methods of Analysis: Principles, instrumentation and applications of T5
ThermogravimetricAnalysis (TGA), Differential Thermal Analysis (DTA),
Differential Scanning Calorimetry (DSC).
2 X-Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, X- ray T5
Crystallography, rotating crystal technique, single crystal diffraction, powder
diffraction, structural elucidation and applications.
3 Calibration and validation-as per ICH and USFDA guidelines: Calibration of
R3
following Instruments; Electronic balance, UV-Visible spectrophotometer, IR
spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC.
4 Hyphenated techniques: LC-MS/MS, GC-MS/MS, HPTLC-MS. R3

*ST3 (Syllabus Covered till date or as decided by Course Coordinator with due approval from
Dean of the school)
9. Details of all evaluation components:
Evaluation Type of Component No. of Weightage of Mode of Assessment
Component Assessments Component

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Assignment 02 40% Offline

Component 1

Sessional Test 02 60% offline

Component 2

Quiz Test 02 - Offline

Component 3

Total 100%

10. Any other important details: Kindly mention the other important details necessary for smooth running of the
course in bullet points

11. Summary of the course topics to be covered in brief:

Contents Number of
contact hours
Unit 1 Nuclear Magnetic Resonance spectroscopy: Principles of H-NMR and C-NMR,
08
chemical shift, factors affecting chemical shift, coupling constant, Spin - spin coupling,
relaxation, instrumentation and applications

Unit 2 Mass Spectrometry: Principles, Fragmentation, Ionization techniques – Electron impact, 09

chemical-ionization, MALDI, FAB, Analyzers-Time of flight and Quadrupole, instrumentation,
applications.

Unit 3 Thermal Methods of Analysis: Principles, instrumentation and applications of 04

ThermogravimetricAnalysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning
Calorimetry (DSC).

Unit 4 X-Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, X- ray 07
Crystallography, rotating crystal technique, single crystal diffraction, powder diffraction,
structural elucidation and applications.

Unit 5 Calibration and validation-as per ICH and USFDA guidelines: Calibration of following
06
Instruments; Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer,
Fluorimeter, Flame Photometer, HPLC and GC.

Unit 6 Radio immune assay: Importance, various components, Principle, different methods, 04
Limitation and Applications of Radio immuno assay.
Unit 7 Extraction techniques: General principle and procedure involved in the solid phase 05
extraction and liquid-liquid extraction.
Unit 8 Hyphenated techniques: LC-MS/MS, GC-MS/MS, HPTLC-MS. 07
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12. Course execution plan:

Lect. No. Topics Due date of lecture Deviation from the plan, if any with
(Hour) Delivery remarks
07 Nuclear Magnetic Resonance
spectroscopy: Principles of H-NMR and
C-NMR, chemical shift, factors affecting
chemical shift, coupling constant, Spin -
spin coupling, relaxation,
instrumentation and applications
09 Mass Spectrometry: Principles,
Fragmentation, Ionization techniques –
Electron impact, chemical-ionization,
MALDI, FAB, Analyzers-Time of flight
and Quadrupole, instrumentation,
applications.
08 Thermal Methods of Analysis:
Principles, instrumentation and
applications of
ThermogravimetricAnalysis (TGA),
Differential Thermal Analysis (DTA),
Differential Scanning Calorimetry
(DSC).

07 X-Ray Diffraction Methods: Origin of

X-rays, basic aspects of crystals, X- ray
Crystallography, rotating crystal
technique, single crystal diffraction,
powder diffraction, structural
elucidation and applications.
08 Calibration and validation-as per ICH
and USFDA guidelines: Calibration of
following Instruments; Electronic
balance, UV-Visible spectrophotometer,
IR spectrophotometer, Fluorimeter,
Flame Photometer, HPLC and GC.
03 Radio immune assay: Importance,
various components, Principle,
different methods, Limitation and
Applications of Radio immuno assay.
05 Extraction techniques: General
principle and procedure involved in the
solid phase extraction and liquid-liquid
extraction.
Hyphenated techniques: LC-MS/MS,
07 GC-MS/MS, HPTLC-MS.

13. Academic Honesty policy:

Chitkara University ensures the implementation of the highest level of academic integrity in all the documents
being prepared / adopted by its Faculty members and students.
Any breach of the same will be tantamount to severe academic penalties.

14. This Document is approved by:

Designation Name Signature

Course Coordinator Dr. Anju Goyal

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Program In-charge Dr. Anju Goyal

Dean
DD/MM/YYYY

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