Course Curriculum (w.e.f.

Session 2020-21)
Doctor of Philosophy - Biotechnology/Microbiology & Immunology

PBS 1002: BIOINFORMATICS AND INSTRUMENTATION

OBJECTIVES: The course aims to provide an advanced understanding of the core principles
and topics of bioinformatics to increase the understanding of biological processes and
instrumentation helps researchers, technician, and students to identify diagnose the problem and
provide their solutions. Bioinformatics helps in developing and applying computationally
intensive techniques to enable the discovery of new biological insights as well as to create a
global perspective from which unifying principles in biology can be discerned. Instrumentation
includes all those measures which are used during the research to collect data. The quality of
research depends to a large extent on the quality of the research instrument. The more efficient
the research instrument, the more authentic the research becomes.

Credits: 04 Semester I L–T–P: 4–0–0

Module Teaching
Content
No. Hours
Principles of DNA and Protein sequencing, file formats for storage of
Sequence and Structural Data, Primary Sequence Databases of Nucleic
Acids and Proteins, Organism Specific Genome Databases, Structural
Databases.
I Specialized Sequence Databases of Expressed Sequence Tags, Gene 14
Expression, Single Nucleotide Polymorphism, OMIM, Unigene etc., Data
Retrieval with ENTERZ, SRG and DBGET, Secondary Databases (Pfam,
PROSITE, PRINT, BLOCK etc.)

Chromatography: Adsorption and partition, theory, preparation, procedure

and methods of detection, Column, Thin layer chromatography, Paper
chromatography.
Gas chromatography: Introduction, fundamentals, instrumentation, columns,
preparation and operation, detection, dramatization.
HPLC: Principals and instrumentation, solvent and columns used, detection
and applications.
Electrophoresis.
II Immunoassay Techniques. 14
UV-Visible spectroscopy, introduction, electromagnetic spectrum,
absorbance laws and limitation, instrumentation design and working
principle, chromophore concept, auxochromes, wood-fisher rules for
calculating absorption maximum application of UV-visible spectroscopy.
IR spectroscopy: Basic principles- Molecular vibrations, vibrational
frequency, factor influencing vibrational frequencies sampling techniques,
instrumentation interpretation of spectra, FT-IR, theory and applications.

Mass spectroscopy: Theory, ionization technique: electron impact ionization

III 14
chemical ionization field ionization fast atom bombardment plasma

DEPARTMENT OF BIOTECHNOLOGY, INSTITUTE OF APPLIED SCIENCE & HUMANITIES (IAH)

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 Course Curriculum (w.e.f. Session 2020-21)
Doctor of Philosophy - Biotechnology/Microbiology & Immunology

desorption fragmentation process type of fission resolution.

NMR: Theory, instrumentation, chemical shift, shielding and deshielding
effect, splitting of signals, spin-spin coupling, proton exchange reactions,
coupling constant (J), 13CNMR spectra and its applications.

TEXT BOOK:
 Instrumental Method of Chemical Analysis by B.K. Sharma.
 Organic spectroscopy by Y.R. Sharma

REFERENCE BOOKS:
 A Text book of Pharmaceutical Analysis by Kerrenth A. Connors.
 Vogel’s Text book of Quantitive Chemical Analysis by A.I. Vogel.
 Practical Pharmaceutical Chemistry by A.H. Beckett and J.B. Stenlake.
 Organic Chemistry by I.L. Finar Organic spectroscopy by William KempQuantitative.
 Analysis of drugs by D.C. Garraett.
 Quantitative analysis of Drugs in Pharmaceutical Formulations by P.D. Sethi.
 Spectrophotometric identification of Organic Compound by Silverstein

Focus: This course focuses on employability and skill development.

OUTCOME:
CO1: Understand the principles of methodology of different DNA and Protein sequencing
methods. (Remembering, Understand, Analyze)
CO2: Understand about different file formats for storage of Nucleic acid and protein Sequences
and Structural Data. (Remembering, Remembering, Apply, Analyze)
CO3: Understand about Primary Sequence Databases of Nucleic Acids and Proteins.
(Remembering, Understand, Analyze)
CO4. Understand about Organism Specific Genome Databases and Structural Databases.
(Remembering, Understand, Analyze)
CO5. Understand about Specialized Sequence Databases of Expressed Sequence Tags
(Remembering, Understand, Analyze)
CO6. Understand about Gene Expression, Single Nucleotide Polymorphism (Remembering,
Understand, Analyze)
CO7. Understand about OMIM, Unigene etc (Remembering, Understand, Analyze)
CO8. Understand about Data Retrieval with ENTERZ, SRG and DBGET (Remembering,
Understand, Analyze)
CO9. Understand about different chromatographic techniques like Column, Thin layer, Paper,
Gas and HPLC chromatography and it’s application. (Remembering, Understand, Apply and
Analyze)
CO10. Understand about different types of Electrophoresis and it’s applications. (Remembering,
Understand, Apply and Analyze)
CO11. Understand about different types of Spectroscopy (UV-Visible spectroscopy, IR
spectroscopy, Mass spectroscopy) and it’s applications. (Remembering, Understand, Apply and
Analyze)

DEPARTMENT OF BIOTECHNOLOGY, INSTITUTE OF APPLIED SCIENCE & HUMANITIES (IAH)

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 Course Curriculum (w.e.f. Session 2020-21)
Doctor of Philosophy - Biotechnology/Microbiology & Immunology

CO12. Understand about Nuclear magnetic resonance (NMR) spectroscopy and its applications.
(Remembering, Understand, Apply and Analyze)

PBS 1003: BIOLOGICAL CHEMISTRY

OBJECTIVES: The course aims to provide an advanced understanding of the core principles
and topics of Biochemistry, Advanced Immunology, Microbiology and Molecular Biology,

DEPARTMENT OF BIOTECHNOLOGY, INSTITUTE OF APPLIED SCIENCE & HUMANITIES (IAH)

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 Course Curriculum (w.e.f. Session 2020-21)
Doctor of Philosophy - Biotechnology/Microbiology & Immunology

Translational research on medicinal plants and their experimental basis, and to enable students to
acquire a specialized knowledge and understanding of selected aspects by means of lecture
series.

Credits: 04 Semester I L–T–P: 4–0–0

Module Teaching
Content
No. Hours
a. Chemistry of life
b. Mechanism in biological Chemistry
c. Porphyrin’s structure determination of Hemoglobin’s chlorophyll
d. Enzymes and their kinetics
I e. Purines & pyrimidines 14
f. Nucleosides, Nucleotides
g. DNA & RNA Molecules
h. Amino Acid & Proteins
a. Protein Biosynthesis
b. Nucleic acid synthesis
c. Carbohydrates & Glyco- proteins
II 14
d. Metabolism of Carbohydrates and proteins
e. Signaling molecules (Extra cellular & intra cellular)
f. Cytokines
a. Conventional and molecular based techniques for diagnosis of infectious
diseases.
b. Cytokines chemokines and growth factors for regulation of mechamjms of
immune responses. Prospects of cytokine therapy in treatment of cancer and
immunological disorders.
c. Mycobacterium infections distinguishing and other chasacterstics of
III differvent species of genus mycobacterium modern techniques for their 14
epidemiological studies.
d. Immunoassays.
e. Stem cells and stem cell thexapy.
f. Invitrofertilization and its significance in present scenario.
g. Traditional systems of medicine and need of scientific validation to
compete modem medicine.

TEXT BOOK:
 Harper’s illustrated Biochemitry Robert K. Murray; Mc. Graw Hill.
 Fundamental of Biochemistry; J.L. Jain & Sanjay Jain, S. Chand.

REFERENCE BOOKS:
 Biochemistry; U. Satyanarayan, Books and Allied (P) Ltd..
 Lehniger Principles of Biochemistry
 David L. Nelsan & Michael M. Cox Freeman.NMS: Biochemistry Davidson, Victor.
LB.I. Wavertey Pvt.
 Algae Biology & Biotechnology; O.P. Singh & Gurpeet Singh & Jis Khattar I.K.
International Publishing house.

DEPARTMENT OF BIOTECHNOLOGY, INSTITUTE OF APPLIED SCIENCE & HUMANITIES (IAH)

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 Course Curriculum (w.e.f. Session 2020-21)
Doctor of Philosophy - Biotechnology/Microbiology & Immunology

 Pharmacogonosy; C.K. Kokate & A.P. Purohit; Nirali Prakashan

 Pharmacogonosy; W.C. Evans; Elsevier.
 Bioinformatics Sequence and Gename Analysis David W. Mount; CBS Publisher.
 Bioinformatics concept, Skell & Applications S.C. Rastogi, Namita Mendisatta CBS
Publications.
 Bioinformatics; M.M. Ranga, Agrobios (India)
 Instrumental method of chemical analysis Gurdeep R. Chatwat & Sham K. Willand
Merrit Dea Himalaya Publishing house.
 Spectroscopy of organic compound P.S. Kalsi; New Age International Publisher.
 Analytical Instrumentation; Gilian Mcmohan; Wiley International

Focus: This course focuses on employability.

OUTCOME: The major outcomes of this course are:
CO1: Understand the degradative pathways (Glycolysis & TCA cycle) of carbohydrate
metabolism, various pathways for biosynthesis and degradation of amino acids. (Remembering,
Understand, Analyze)
CO2: Understand different types of fatty acid oxidation, energy yields and its regulation. (Apply,
Remembering, Analyze)
CO3: Understand De-novo and Salvage pathway of nucleotide biosynthesis with regulation and
to know about various steps of degradation of purine and pyrimidine nucleotides.
(Remembering, Understand, Apply, Analyze)
CO4. Understand complexity and organization of genome in different organism and their repair
mechanism. (Remembering, Understand, Analyze)
CO5. Analyze the genetic code and describe the Translation and post translation modification
process. (Understand and Analyse)
CO6. Illustrate various mechanisms that regulate immune responses and knowledge of
immunization. (Understand and Analyse)
CO7. Understand the extraction processes, medicinal values and scientific validation of herbal
plants and products. (Remembering, Understand, Analyze)

DEPARTMENT OF BIOTECHNOLOGY, INSTITUTE OF APPLIED SCIENCE & HUMANITIES (IAH)

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